Click here to switch to the map view.

The map label for this gene is ldhB [H]

Identifier: 125623261

GI number: 125623261

Start: 384757

End: 385701

Strand: Reverse

Name: ldhB [H]

Synonym: llmg_0392

Alternate gene names: 125623261

Gene position: 385701-384757 (Counterclockwise)

Preceding gene: 125623262

Following gene: 125623259

Centisome position: 15.25

GC content: 35.03

Gene sequence:

>945_bases
ATGAAAATTACAAGTAGAAAAGTAGTCGTGATTGGAACAGGATTTGTCGGAACAAGTATTGCTTATTCAATGATTAATCA
AGGGCTTGTCAACGAGTTAGTTTTGATAGATGTCAATCAAGATAAAGCTGAGGGTGAGGCTTTAGATTTATTGGATGGCA
TATCTTGGGCTCAAGAAAATGTTATTGTAAGAGCTGGAAATTACAAGGATTGTGAAAATGCAGATATTGTCGTGATTACA
GCTGGTGTGAACCAAAAACCCGGACAATCACGTTTAGATTTGGTCAATACAAATGCTAAAATTATGCGCTCAATCGTTAC
TCAAGTTATGGATTCAGGTTTTGATGGTATTTTTGTAATTGCTTCTAATCCTGTGGATATCCTTACCTATGTCGCTTGGG
AAACTTCTGGTCTTGACCAATCTAGAATTGTCGGAACTGGTACAACTTTGGATACCACACGTTTTAGAAAAGAATTGGCC
ACAAAACTAGAAATTGACCCTCGGAGTGTTCACGGATATATTATTGGTGAGCATGGCGATTCTGAAGTTGCAGTTTGGTC
ACACACTACGATAGGTGGAAAACCTATTCTTGAATTTATTGTTAAAAATAAAAAAATTGGTCTTGAAGATTTGTCTAATT
TGTCAAATAAGGTTAAAAATGCTGCTTATGAAATTATTGATAAAAAACAAGCCACTTATTACGGAATTGGAATGAGCACA
GCTAGAATTGTCAAAGCCATATTAAATAATGAACAAGTCATTCTACCAGTTTCTGCCTATTTGCGTGGAGAATATGGTCA
AGAAGGGGTCTTTACTGGTGTTCCATCTGTCGTAAATCAAAATGGTGTCAGAGAAATTATTGAACTCAATATTGATGCTT
ATGAAATGAAACAATTTGAAAAGTCTGTCAGTCAACTTAAAGAAGTCATAGAATCTATTAAATAA

Upstream 100 bases:

>100_bases
TTTATGCACTATGATTAGTTATCTATGGATTTTTAACAGATAGGTTTTATAAAAATATAGAAAAAATATTGATTAATAAA
AAATCGAAAAGGAGATAAAA

Downstream 100 bases:

>100_bases
TCTAGTTAGGTTTTACAATAGTAAAGGAAAATAAAATGATCGCTCTACTCTTTTTAGTCATTGCTTCACTTTGGGGATTA
ATCTGGTTTAACATTAGTGA

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 2 [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKITSRKVVVIGTGFVGTSIAYSMINQGLVNELVLIDVNQDKAEGEALDLLDGISWAQENVIVRAGNYKDCENADIVVIT
AGVNQKPGQSRLDLVNTNAKIMRSIVTQVMDSGFDGIFVIASNPVDILTYVAWETSGLDQSRIVGTGTTLDTTRFRKELA
TKLEIDPRSVHGYIIGEHGDSEVAVWSHTTIGGKPILEFIVKNKKIGLEDLSNLSNKVKNAAYEIIDKKQATYYGIGMST
ARIVKAILNNEQVILPVSAYLRGEYGQEGVFTGVPSVVNQNGVREIIELNIDAYEMKQFEKSVSQLKEVIESIK

Sequences:

>Translated_314_residues
MKITSRKVVVIGTGFVGTSIAYSMINQGLVNELVLIDVNQDKAEGEALDLLDGISWAQENVIVRAGNYKDCENADIVVIT
AGVNQKPGQSRLDLVNTNAKIMRSIVTQVMDSGFDGIFVIASNPVDILTYVAWETSGLDQSRIVGTGTTLDTTRFRKELA
TKLEIDPRSVHGYIIGEHGDSEVAVWSHTTIGGKPILEFIVKNKKIGLEDLSNLSNKVKNAAYEIIDKKQATYYGIGMST
ARIVKAILNNEQVILPVSAYLRGEYGQEGVFTGVPSVVNQNGVREIIELNIDAYEMKQFEKSVSQLKEVIESIK
>Mature_314_residues
MKITSRKVVVIGTGFVGTSIAYSMINQGLVNELVLIDVNQDKAEGEALDLLDGISWAQENVIVRAGNYKDCENADIVVIT
AGVNQKPGQSRLDLVNTNAKIMRSIVTQVMDSGFDGIFVIASNPVDILTYVAWETSGLDQSRIVGTGTTLDTTRFRKELA
TKLEIDPRSVHGYIIGEHGDSEVAVWSHTTIGGKPILEFIVKNKKIGLEDLSNLSNKVKNAAYEIIDKKQATYYGIGMST
ARIVKAILNNEQVILPVSAYLRGEYGQEGVFTGVPSVVNQNGVREIIELNIDAYEMKQFEKSVSQLKEVIESIK

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI291575128, Length=317, Percent_Identity=39.4321766561514, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI4557032, Length=317, Percent_Identity=39.4321766561514, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI15082234, Length=313, Percent_Identity=39.6166134185303, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=38.6885245901639, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=38.6885245901639, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI47059044, Length=317, Percent_Identity=37.8548895899054, Blast_Score=224, Evalue=5e-59,
Organism=Homo sapiens, GI221136809, Length=317, Percent_Identity=37.8548895899054, Blast_Score=224, Evalue=5e-59,
Organism=Homo sapiens, GI5031857, Length=305, Percent_Identity=36.7213114754098, Blast_Score=215, Evalue=3e-56,
Organism=Homo sapiens, GI260099723, Length=305, Percent_Identity=36.7213114754098, Blast_Score=215, Evalue=4e-56,
Organism=Homo sapiens, GI260099727, Length=187, Percent_Identity=42.7807486631016, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI260099725, Length=187, Percent_Identity=42.7807486631016, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI207028494, Length=208, Percent_Identity=34.6153846153846, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI103472011, Length=288, Percent_Identity=26.7361111111111, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI103472015, Length=184, Percent_Identity=27.1739130434783, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI21735621, Length=319, Percent_Identity=24.7648902821317, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1789632, Length=325, Percent_Identity=29.8461538461538, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535107, Length=294, Percent_Identity=36.734693877551, Blast_Score=207, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI17554310, Length=332, Percent_Identity=28.9156626506024, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17136226, Length=316, Percent_Identity=38.6075949367089, Blast_Score=232, Evalue=3e-61,
Organism=Drosophila melanogaster, GI45550422, Length=313, Percent_Identity=31.629392971246, Blast_Score=165, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24647881, Length=320, Percent_Identity=28.125, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24663599, Length=314, Percent_Identity=24.203821656051, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24663595, Length=305, Percent_Identity=24.9180327868852, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 34399; Mature: 34399

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITSRKVVVIGTGFVGTSIAYSMINQGLVNELVLIDVNQDKAEGEALDLLDGISWAQEN
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHHCCHHCCC
VIVRAGNYKDCENADIVVITAGVNQKPGQSRLDLVNTNAKIMRSIVTQVMDSGFDGIFVI
EEEEECCCCCCCCCCEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEE
ASNPVDILTYVAWETSGLDQSRIVGTGTTLDTTRFRKELATKLEIDPRSVHGYIIGEHGD
ECCCHHEEEEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCC
SEVAVWSHTTIGGKPILEFIVKNKKIGLEDLSNLSNKVKNAAYEIIDKKQATYYGIGMST
CEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHH
ARIVKAILNNEQVILPVSAYLRGEYGQEGVFTGVPSVVNQNGVREIIELNIDAYEMKQFE
HHHHHHHHCCCEEEEEEHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHCCCCHHHHHHHH
KSVSQLKEVIESIK
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKITSRKVVVIGTGFVGTSIAYSMINQGLVNELVLIDVNQDKAEGEALDLLDGISWAQEN
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHHCCHHCCC
VIVRAGNYKDCENADIVVITAGVNQKPGQSRLDLVNTNAKIMRSIVTQVMDSGFDGIFVI
EEEEECCCCCCCCCCEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEE
ASNPVDILTYVAWETSGLDQSRIVGTGTTLDTTRFRKELATKLEIDPRSVHGYIIGEHGD
ECCCHHEEEEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCC
SEVAVWSHTTIGGKPILEFIVKNKKIGLEDLSNLSNKVKNAAYEIIDKKQATYYGIGMST
CEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHH
ARIVKAILNNEQVILPVSAYLRGEYGQEGVFTGVPSVVNQNGVREIIELNIDAYEMKQFE
HHHHHHHHCCCEEEEEEHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHCCCCHHHHHHHH
KSVSQLKEVIESIK
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]