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The map label for this gene is rlrD [H]

Identifier: 125623262

GI number: 125623262

Start: 386016

End: 386837

Strand: Reverse

Name: rlrD [H]

Synonym: llmg_0393

Alternate gene names: 125623262

Gene position: 386837-386016 (Counterclockwise)

Preceding gene: 125623267

Following gene: 125623261

Centisome position: 15.29

GC content: 30.9

Gene sequence:

>822_bases
ATGTTTTCATTATTTGAGACTTTTATCGTTGTTTATGAAACTAAAAGTTTTACCTTAGCCGGAAAATATTTATTTATTTC
ACAACCTACTGTAACCGTGAGAATCAAAAAACTTGAAGAAGAATTAAAATCAACATTATTTCTAAGGGGAAAACACCAAG
AAATCATTCCGACTGAAGCCGCAATATTATTTTATCCCAAAGCGATTGCCTATCTAAAAAAATGGGAGGAAGACCAAGCG
GAGGTACAAAAGAAATCTCTTGCAAAACATCCTTTCAAAATCGGAGTTTCACATAGTGCTGCTTTAAGTATTATGCCAAG
AATTTTTAAAGTATTTGAAGATGAGTTGGAACAATTGGATGTAGAAATTAATATGTATGACTCAGAAAAAGTATTTGAAT
TAGTTGCAAATCATGATTTGCATTTTGGAATCATTGAACGATTACTCATTAGTGATCAAACCGAAACCTTTCCTTTGTTT
TTAGATGAGTTGGTCCTTGCTGGAAATACAGATTCAGAAACTTTCTTTACCAGAGAATCTGGTTCAGGAATTGGATATTA
TATCAAAAGATATCTCAAATCTGCTCCTTCTTCTAGAAGAAACATTGTTCGTATGAATAGTAATGAGATGATTATTTCTC
ATATCAAAGCTGGTTTAGGTTCTTCTTTGATTTCTAAATCCTTTTTATCTGATGATATTCCTTATCAAGAACTTGGAAGT
AATTATCATAGAGAGTTTTTAGGAGTTTCTTTTGATGAAGAAAAAGACCCCGTGATTCAAAAATTAATTAATAAAATAAA
AAAAGAAACCGAAATTCGTTAG

Upstream 100 bases:

>100_bases
ATAATATATAATAGTTGAATGAATTTGTAAAATAAATTATTATAATCATATGAATTAAAAAAAATAATTTGTTAATGCCA
TGTTTTAAGGAGAAAATCAG

Downstream 100 bases:

>100_bases
AAATAATAAAAATAAGAAACAGAAGCAAGCTGCTTCTGCTTTTTTGTTTTTATTATTTTTTTTAATCATAATGATAAAAT
AAATTCATTTTACACATTGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MFSLFETFIVVYETKSFTLAGKYLFISQPTVTVRIKKLEEELKSTLFLRGKHQEIIPTEAAILFYPKAIAYLKKWEEDQA
EVQKKSLAKHPFKIGVSHSAALSIMPRIFKVFEDELEQLDVEINMYDSEKVFELVANHDLHFGIIERLLISDQTETFPLF
LDELVLAGNTDSETFFTRESGSGIGYYIKRYLKSAPSSRRNIVRMNSNEMIISHIKAGLGSSLISKSFLSDDIPYQELGS
NYHREFLGVSFDEEKDPVIQKLINKIKKETEIR

Sequences:

>Translated_273_residues
MFSLFETFIVVYETKSFTLAGKYLFISQPTVTVRIKKLEEELKSTLFLRGKHQEIIPTEAAILFYPKAIAYLKKWEEDQA
EVQKKSLAKHPFKIGVSHSAALSIMPRIFKVFEDELEQLDVEINMYDSEKVFELVANHDLHFGIIERLLISDQTETFPLF
LDELVLAGNTDSETFFTRESGSGIGYYIKRYLKSAPSSRRNIVRMNSNEMIISHIKAGLGSSLISKSFLSDDIPYQELGS
NYHREFLGVSFDEEKDPVIQKLINKIKKETEIR
>Mature_273_residues
MFSLFETFIVVYETKSFTLAGKYLFISQPTVTVRIKKLEEELKSTLFLRGKHQEIIPTEAAILFYPKAIAYLKKWEEDQA
EVQKKSLAKHPFKIGVSHSAALSIMPRIFKVFEDELEQLDVEINMYDSEKVFELVANHDLHFGIIERLLISDQTETFPLF
LDELVLAGNTDSETFFTRESGSGIGYYIKRYLKSAPSSRRNIVRMNSNEMIISHIKAGLGSSLISKSFLSDDIPYQELGS
NYHREFLGVSFDEEKDPVIQKLINKIKKETEIR

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=244, Percent_Identity=25.8196721311475, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31450; Mature: 31450

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSLFETFIVVYETKSFTLAGKYLFISQPTVTVRIKKLEEELKSTLFLRGKHQEIIPTEA
CHHHHEEEEEEEECCCEEEEEEEEEEECCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCH
AILFYPKAIAYLKKWEEDQAEVQKKSLAKHPFKIGVSHSAALSIMPRIFKVFEDELEQLD
HEEEHHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHC
VEINMYDSEKVFELVANHDLHFGIIERLLISDQTETFPLFLDELVLAGNTDSETFFTRES
EEEEEECHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECC
GSGIGYYIKRYLKSAPSSRRNIVRMNSNEMIISHIKAGLGSSLISKSFLSDDIPYQELGS
CCCHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCHHHHHHHHHCCCCCHHHHCC
NYHREFLGVSFDEEKDPVIQKLINKIKKETEIR
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFSLFETFIVVYETKSFTLAGKYLFISQPTVTVRIKKLEEELKSTLFLRGKHQEIIPTEA
CHHHHEEEEEEEECCCEEEEEEEEEEECCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCH
AILFYPKAIAYLKKWEEDQAEVQKKSLAKHPFKIGVSHSAALSIMPRIFKVFEDELEQLD
HEEEHHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHC
VEINMYDSEKVFELVANHDLHFGIIERLLISDQTETFPLFLDELVLAGNTDSETFFTRES
EEEEEECHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECC
GSGIGYYIKRYLKSAPSSRRNIVRMNSNEMIISHIKAGLGSSLISKSFLSDDIPYQELGS
CCCHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCHHHHHHHHHCCCCCHHHHCC
NYHREFLGVSFDEEKDPVIQKLINKIKKETEIR
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]