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The map label for this gene is rlrG [H]

Identifier: 125623259

GI number: 125623259

Start: 382562

End: 383380

Strand: Reverse

Name: rlrG [H]

Synonym: llmg_0390

Alternate gene names: 125623259

Gene position: 383380-382562 (Counterclockwise)

Preceding gene: 125623261

Following gene: 125623258

Centisome position: 15.16

GC content: 33.82

Gene sequence:

>819_bases
ATGTTCGCTTTACTCGAAACTTTTATTGCTGTGTTTGAAACCAAAAGCTTTACCAGAGCTGCTAGTCAATGTTTTATTTC
ACAACCAACCGCAACTGTTAGAATTAAAAAATTGGAGGAAGAACTAAAGGTTCAACACTTTTCAAGAGGGCAACACCAAG
AAGTAATTCCCACAGAAAGTGCCCATCTTCTTTATCCTAAAGCTTTAAAAACGCTCAATGATTGGAATGAATTACAATTT
TCCTTAAAAAATCAGACGCCTAGTCGTAAACCTTTTAAAATTGCTGCTTCTCATAGTTCAGCAACTACTGTTCTCCCTCT
GATTTTCAAAAATTTAATTCCTGAACTGGAACAACTTAATATTGAATTGATGATGCTTGATTCTCAAGAGGTTTTTGATT
TAATTCGTAATCATGAAATTCATTTTGGAATTATTGAAAAACCGATGATGGATGAAACCATCGAAACTTTTCCACTCTTT
AAAGACGAGCTCGTTCTTGCTGGCAAGCCTGAAAATGATATTTTTTTTATTCGTGAAGCGGGGTCCGGGGTGGCTCACTA
TACGCATAATTATCTTAAAGAAAATAATCTTAATCCGAAAAACTTAGTCAGCGTCAATAATAATGATATGATTGTCAGCC
AACTCAATGCTGGAATTGGTGTGTCATTAATTTCCAAACGATTTATTAATGAAAACATTGCTTTTCAAAGTCTTGACGAA
CGTTATCACCGCATTTTTTATGGTGTTTCTTTCTTGGCCGAACAAGATAAATTGCTCAAAACTGTGATTAAACAAATTAA
AAATTTAAGTGAATTCTAA

Upstream 100 bases:

>100_bases
TTAAATTGTCTAGCATCAATTAGTTTACACAATCTCCTTTATTTTGTAAAATAAATTATTATCATTTTTAAAATTACTTT
TATTAATTAAAGGAAGATTT

Downstream 100 bases:

>100_bases
AAAATTTACTGACAGATTTTTTTATTGCCACTCATCATTTTATAAATTTCTGTCAGTAATTTTGTAAGTATTTTTTAATG
ACAGCAAAAAAATCAGTTTA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MFALLETFIAVFETKSFTRAASQCFISQPTATVRIKKLEEELKVQHFSRGQHQEVIPTESAHLLYPKALKTLNDWNELQF
SLKNQTPSRKPFKIAASHSSATTVLPLIFKNLIPELEQLNIELMMLDSQEVFDLIRNHEIHFGIIEKPMMDETIETFPLF
KDELVLAGKPENDIFFIREAGSGVAHYTHNYLKENNLNPKNLVSVNNNDMIVSQLNAGIGVSLISKRFINENIAFQSLDE
RYHRIFYGVSFLAEQDKLLKTVIKQIKNLSEF

Sequences:

>Translated_272_residues
MFALLETFIAVFETKSFTRAASQCFISQPTATVRIKKLEEELKVQHFSRGQHQEVIPTESAHLLYPKALKTLNDWNELQF
SLKNQTPSRKPFKIAASHSSATTVLPLIFKNLIPELEQLNIELMMLDSQEVFDLIRNHEIHFGIIEKPMMDETIETFPLF
KDELVLAGKPENDIFFIREAGSGVAHYTHNYLKENNLNPKNLVSVNNNDMIVSQLNAGIGVSLISKRFINENIAFQSLDE
RYHRIFYGVSFLAEQDKLLKTVIKQIKNLSEF
>Mature_272_residues
MFALLETFIAVFETKSFTRAASQCFISQPTATVRIKKLEEELKVQHFSRGQHQEVIPTESAHLLYPKALKTLNDWNELQF
SLKNQTPSRKPFKIAASHSSATTVLPLIFKNLIPELEQLNIELMMLDSQEVFDLIRNHEIHFGIIEKPMMDETIETFPLF
KDELVLAGKPENDIFFIREAGSGVAHYTHNYLKENNLNPKNLVSVNNNDMIVSQLNAGIGVSLISKRFINENIAFQSLDE
RYHRIFYGVSFLAEQDKLLKTVIKQIKNLSEF

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=252, Percent_Identity=27.7777777777778, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31256; Mature: 31256

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFALLETFIAVFETKSFTRAASQCFISQPTATVRIKKLEEELKVQHFSRGQHQEVIPTES
CHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
AHLLYPKALKTLNDWNELQFSLKNQTPSRKPFKIAASHSSATTVLPLIFKNLIPELEQLN
CEEECHHHHHHHCCHHHHEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHCC
IELMMLDSQEVFDLIRNHEIHFGIIEKPMMDETIETFPLFKDELVLAGKPENDIFFIREA
EEEEEECCHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEC
GSGVAHYTHNYLKENNLNPKNLVSVNNNDMIVSQLNAGIGVSLISKRFINENIAFQSLDE
CCCHHHHHHHHHHCCCCCHHHEEEECCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHH
RYHRIFYGVSFLAEQDKLLKTVIKQIKNLSEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFALLETFIAVFETKSFTRAASQCFISQPTATVRIKKLEEELKVQHFSRGQHQEVIPTES
CHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
AHLLYPKALKTLNDWNELQFSLKNQTPSRKPFKIAASHSSATTVLPLIFKNLIPELEQLN
CEEECHHHHHHHCCHHHHEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHCC
IELMMLDSQEVFDLIRNHEIHFGIIEKPMMDETIETFPLFKDELVLAGKPENDIFFIREA
EEEEEECCHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEC
GSGVAHYTHNYLKENNLNPKNLVSVNNNDMIVSQLNAGIGVSLISKRFINENIAFQSLDE
CCCHHHHHHHHHHCCCCCHHHEEEECCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHH
RYHRIFYGVSFLAEQDKLLKTVIKQIKNLSEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]