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The map label for this gene is mtlF [H]

Identifier: 125622905

GI number: 125622905

Start: 30358

End: 30789

Strand: Direct

Name: mtlF [H]

Synonym: llmg_0024

Alternate gene names: 125622905

Gene position: 30358-30789 (Clockwise)

Preceding gene: 125622904

Following gene: 125622906

Centisome position: 1.2

GC content: 34.03

Gene sequence:

>432_bases
ATGGAAATCCAAAAAGAGCTTATTAAAATCAATCAAAATTTTTCAAATAAGGAAGAAGCAATTAATTATTGTGGAAAATT
GTTGGTTGATGGAGGATACGTCAACTCAAATTATGTTTCAGCGATGCTTGATAGAGATAAAGAACTATCGGTTTATATGG
GCAATTTTATCGCGATTCCTCATGGGAAGGATTCAGCAAAAAAAGAAGTTTTAAAAACAGGAGTCGTAATTGTCCAAGTT
CCTCGAGGAGTAAATTTTGGAACAGAAGAAAATCCGAAACTAGCAACGATGCTTTTTGGAATCGCAGGAATAGAAGATGA
GCATCTTGATCTCATTCAAAAAATCTCAATTTTTTGTGCTGACGTTGACAATGTTGTAAAGCTGGCTGATGCTCAAACAG
TTGAGGAGATTGCAAAATACTTTGAAAATTAA

Upstream 100 bases:

>100_bases
AGTGAGTTGCTAAGGGAAATAATCATAGAAGCTGTTAAACAGTATGGTGATTAGGTTTACGGCGTGTGTAAACCTAGTAA
ACCAAGAAAGGTGAAGAAAA

Downstream 100 bases:

>100_bases
TCAATAAAATCAAAAAAATGGCACAACTTTCGGTGCCATTTTTTATATTTACCACCACAATGTAAAAGCTTACAATTGCT
TATGTAAAGGGATTTTAGAA

Product: PTS system, mannitol-specific IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Mtl; EIII-Mtl; PTS system mannitol-specific EIIA component [H]

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MEIQKELIKINQNFSNKEEAINYCGKLLVDGGYVNSNYVSAMLDRDKELSVYMGNFIAIPHGKDSAKKEVLKTGVVIVQV
PRGVNFGTEENPKLATMLFGIAGIEDEHLDLIQKISIFCADVDNVVKLADAQTVEEIAKYFEN

Sequences:

>Translated_143_residues
MEIQKELIKINQNFSNKEEAINYCGKLLVDGGYVNSNYVSAMLDRDKELSVYMGNFIAIPHGKDSAKKEVLKTGVVIVQV
PRGVNFGTEENPKLATMLFGIAGIEDEHLDLIQKISIFCADVDNVVKLADAQTVEEIAKYFEN
>Mature_143_residues
MEIQKELIKINQNFSNKEEAINYCGKLLVDGGYVNSNYVSAMLDRDKELSVYMGNFIAIPHGKDSAKKEVLKTGVVIVQV
PRGVNFGTEENPKLATMLFGIAGIEDEHLDLIQKISIFCADVDNVVKLADAQTVEEIAKYFEN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790027, Length=132, Percent_Identity=43.9393939393939, Blast_Score=109, Evalue=8e-26,
Organism=Escherichia coli, GI1788494, Length=130, Percent_Identity=38.4615384615385, Blast_Score=94, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 15914; Mature: 15914

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIQKELIKINQNFSNKEEAINYCGKLLVDGGYVNSNYVSAMLDRDKELSVYMGNFIAIP
CCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEECCEEEEC
HGKDSAKKEVLKTGVVIVQVPRGVNFGTEENPKLATMLFGIAGIEDEHLDLIQKISIFCA
CCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
DVDNVVKLADAQTVEEIAKYFEN
CHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEIQKELIKINQNFSNKEEAINYCGKLLVDGGYVNSNYVSAMLDRDKELSVYMGNFIAIP
CCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEECCEEEEC
HGKDSAKKEVLKTGVVIVQVPRGVNFGTEENPKLATMLFGIAGIEDEHLDLIQKISIFCA
CCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
DVDNVVKLADAQTVEEIAKYFEN
CHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1322373; 12397186 [H]