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The map label for this gene is hyfC [H]

Identifier: 124486435

GI number: 124486435

Start: 1649213

End: 1650082

Strand: Reverse

Name: hyfC [H]

Synonym: Mlab_1623

Alternate gene names: 124486435

Gene position: 1650082-1649213 (Counterclockwise)

Preceding gene: 124486436

Following gene: 124486434

Centisome position: 91.42

GC content: 52.18

Gene sequence:

>870_bases
ATGGATATAATGTTTCTTGTAGGGGCGGTGGCCTTTCTGATACTTGCCCCGATCTTCGGCGGCCTTCTTACCGGATGCGA
CCGGGTACTGACTGCCCGTTTCCAGTCAAGGGTCGGCCCGCCGTTCCTTCAGCCGTTCTACGATGTGGCCAAACTCTGGC
ATAAGGAGAAGGCGTTCTCCAATCCTGTCGAGATCATCTTCACGACGGCATACTTACTCCTGATCGTATTTACCGGAATG
ATGTACGCGACCGGGGGCAGTTTCCTGTTTGTCGTCTTCTCGCTCGCACTCGCCCACACCTTCCTGATCCTTGCCGCGTA
TGCCGCGAACGCTCCGTTCTCCAACATAGGGGCGGAACGTGAACTTCTCGGCGTTATGATCGCCGAACCGATCCTGATCC
TGCTTGCCGTAGGATTCTATATGGTCAGCGGAAGTTTCGAGACATTCCATATGCTGACTCTGGATGTACCAGGAATCGTT
TATCTTCCCGGTGTGTTCATTGCTCTGATTGCGGTCCTGACGCTCAAACTGAGAAAATCGCCGTTCGATATCTCGACCTC
GCACCATGCCCATCAGGAACTGGTGAAAGGCGTGACCACCTCGCTTTCGGGCAGGAGCCTTGCCAAAGTCGAGATCGCCC
ACTGGTACGAGATGATGATCTTCCTTTCCATGATTCTCATCTTCTTTGCCAATTATCCGGGACTTTCCGCAATTGCGATC
GTTCTCGCGTTCCTTCTGGAAATCGTCATCGATAATGTCTTCCCGCGTGTGACCTGGAAGATGACGGTCAAGAGTCTGTG
GATCATCACCCTCGTCTTTGGTGTTGGAAACATTGTCATACTCGGTATCTTTGGAGGTATGTTATTATGA

Upstream 100 bases:

>100_bases
AAGAATACTGCGGTGAGAACGTGCTTCTCAAACCCTCCTGGGTTATCTCGGGAATCCTTCTCATCGTGATGCTTGTTCTC
GGATTTATGGGGGTGATGAT

Downstream 100 bases:

>100_bases
GTTCGGCATCAAAATCCCCCTGGCTTCTCCATTACAATGCGTCCAGCTGTAATGGATGCGATATCGAGATCCTTGCCGCC
TTAACGCCGGTGTACGACGT

Product: ech hydrogenase subunit B

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MDIMFLVGAVAFLILAPIFGGLLTGCDRVLTARFQSRVGPPFLQPFYDVAKLWHKEKAFSNPVEIIFTTAYLLLIVFTGM
MYATGGSFLFVVFSLALAHTFLILAAYAANAPFSNIGAERELLGVMIAEPILILLAVGFYMVSGSFETFHMLTLDVPGIV
YLPGVFIALIAVLTLKLRKSPFDISTSHHAHQELVKGVTTSLSGRSLAKVEIAHWYEMMIFLSMILIFFANYPGLSAIAI
VLAFLLEIVIDNVFPRVTWKMTVKSLWIITLVFGVGNIVILGIFGGMLL

Sequences:

>Translated_289_residues
MDIMFLVGAVAFLILAPIFGGLLTGCDRVLTARFQSRVGPPFLQPFYDVAKLWHKEKAFSNPVEIIFTTAYLLLIVFTGM
MYATGGSFLFVVFSLALAHTFLILAAYAANAPFSNIGAERELLGVMIAEPILILLAVGFYMVSGSFETFHMLTLDVPGIV
YLPGVFIALIAVLTLKLRKSPFDISTSHHAHQELVKGVTTSLSGRSLAKVEIAHWYEMMIFLSMILIFFANYPGLSAIAI
VLAFLLEIVIDNVFPRVTWKMTVKSLWIITLVFGVGNIVILGIFGGMLL
>Mature_289_residues
MDIMFLVGAVAFLILAPIFGGLLTGCDRVLTARFQSRVGPPFLQPFYDVAKLWHKEKAFSNPVEIIFTTAYLLLIVFTGM
MYATGGSFLFVVFSLALAHTFLILAAYAANAPFSNIGAERELLGVMIAEPILILLAVGFYMVSGSFETFHMLTLDVPGIV
YLPGVFIALIAVLTLKLRKSPFDISTSHHAHQELVKGVTTSLSGRSLAKVEIAHWYEMMIFLSMILIFFANYPGLSAIAI
VLAFLLEIVIDNVFPRVTWKMTVKSLWIITLVFGVGNIVILGIFGGMLL

Specific function: Unknown

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI87082115, Length=229, Percent_Identity=30.1310043668122, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789077, Length=303, Percent_Identity=24.4224422442244, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 31779; Mature: 31779

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIMFLVGAVAFLILAPIFGGLLTGCDRVLTARFQSRVGPPFLQPFYDVAKLWHKEKAFS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
NPVEIIFTTAYLLLIVFTGMMYATGGSFLFVVFSLALAHTFLILAAYAANAPFSNIGAER
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHH
ELLGVMIAEPILILLAVGFYMVSGSFETFHMLTLDVPGIVYLPGVFIALIAVLTLKLRKS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
PFDISTSHHAHQELVKGVTTSLSGRSLAKVEIAHWYEMMIFLSMILIFFANYPGLSAIAI
CCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VLAFLLEIVIDNVFPRVTWKMTVKSLWIITLVFGVGNIVILGIFGGMLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDIMFLVGAVAFLILAPIFGGLLTGCDRVLTARFQSRVGPPFLQPFYDVAKLWHKEKAFS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
NPVEIIFTTAYLLLIVFTGMMYATGGSFLFVVFSLALAHTFLILAAYAANAPFSNIGAER
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHH
ELLGVMIAEPILILLAVGFYMVSGSFETFHMLTLDVPGIVYLPGVFIALIAVLTLKLRKS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
PFDISTSHHAHQELVKGVTTSLSGRSLAKVEIAHWYEMMIFLSMILIFFANYPGLSAIAI
CCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VLAFLLEIVIDNVFPRVTWKMTVKSLWIITLVFGVGNIVILGIFGGMLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]