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The map label for this gene is hyfI [H]

Identifier: 124486434

GI number: 124486434

Start: 1648782

End: 1649216

Strand: Reverse

Name: hyfI [H]

Synonym: Mlab_1622

Alternate gene names: 124486434

Gene position: 1649216-1648782 (Counterclockwise)

Preceding gene: 124486435

Following gene: 124486433

Centisome position: 91.37

GC content: 53.33

Gene sequence:

>435_bases
ATGAGTTCGGCATCAAAATCCCCCTGGCTTCTCCATTACAATGCGTCCAGCTGTAATGGATGCGATATCGAGATCCTTGC
CGCCTTAACGCCGGTGTACGACGTCGAGCGGTTTGGGATCATCAATACCGGAAACCCTGCGCACGCCGATATCTTTGTGG
TAACGGGCGGGGTGAACGAACAGAACAAAGTTGTTCTGAAAAATCTCTACGACCAGATCCCCGAACCGAAAGTAGTGGTC
GCGGTCGGAATCTGCGCATCGAGCGGCGGTGTTTTCCGCGACTGCTATAATATTTCCGGCGGCGTTGATACGATCATCCC
GGTGGATGTGTATGTTCCCGGATGTTCCGTCCGTCCCGAGGCGCTTATCGAGGGAATTGTCAAGGCTCTCGGCGTTCTGG
AAGAGAAACGTGCAGCTCTGGAGGCAAAAAAATGA

Upstream 100 bases:

>100_bases
GTGTGACCTGGAAGATGACGGTCAAGAGTCTGTGGATCATCACCCTCGTCTTTGGTGTTGGAAACATTGTCATACTCGGT
ATCTTTGGAGGTATGTTATT

Downstream 100 bases:

>100_bases
ATATGAAAATCGACCCAATACAGGCTGCCGATGTGCAGAAAACCGCCGGCACCATGAGATCCGGCGGCTACCGGCTGGTT
GTCATTACCTGCACGCCGGC

Product: ech hydrogenase subunit C

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 144; Mature: 143

Protein sequence:

>144_residues
MSSASKSPWLLHYNASSCNGCDIEILAALTPVYDVERFGIINTGNPAHADIFVVTGGVNEQNKVVLKNLYDQIPEPKVVV
AVGICASSGGVFRDCYNISGGVDTIIPVDVYVPGCSVRPEALIEGIVKALGVLEEKRAALEAKK

Sequences:

>Translated_144_residues
MSSASKSPWLLHYNASSCNGCDIEILAALTPVYDVERFGIINTGNPAHADIFVVTGGVNEQNKVVLKNLYDQIPEPKVVV
AVGICASSGGVFRDCYNISGGVDTIIPVDVYVPGCSVRPEALIEGIVKALGVLEEKRAALEAKK
>Mature_143_residues
SSASKSPWLLHYNASSCNGCDIEILAALTPVYDVERFGIINTGNPAHADIFVVTGGVNEQNKVVLKNLYDQIPEPKVVVA
VGICASSGGVFRDCYNISGGVDTIIPVDVYVPGCSVRPEALIEGIVKALGVLEEKRAALEAKK

Specific function: Unknown

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=117, Percent_Identity=36.7521367521368, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1788834, Length=143, Percent_Identity=40.5594405594406, Blast_Score=130, Evalue=4e-32,
Organism=Escherichia coli, GI1789074, Length=136, Percent_Identity=41.9117647058824, Blast_Score=129, Evalue=6e-32,
Organism=Escherichia coli, GI1788624, Length=116, Percent_Identity=44.8275862068966, Blast_Score=100, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17509685, Length=119, Percent_Identity=37.8151260504202, Blast_Score=106, Evalue=4e-24,
Organism=Drosophila melanogaster, GI18859983, Length=108, Percent_Identity=38.8888888888889, Blast_Score=106, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24642371, Length=108, Percent_Identity=38.8888888888889, Blast_Score=106, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24651058, Length=118, Percent_Identity=35.5932203389831, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 15261; Mature: 15129

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSASKSPWLLHYNASSCNGCDIEILAALTPVYDVERFGIINTGNPAHADIFVVTGGVNE
CCCCCCCCEEEEECCCCCCCCCEEEEEHHCCHHHHHHCCEEECCCCCCEEEEEEECCCCC
QNKVVLKNLYDQIPEPKVVVAVGICASSGGVFRDCYNISGGVDTIIPVDVYVPGCSVRPE
CCCHHHHHHHHHCCCCEEEEEEEEEECCCCEEEEEECCCCCCCEEEEEEEEECCCCCCHH
ALIEGIVKALGVLEEKRAALEAKK
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSASKSPWLLHYNASSCNGCDIEILAALTPVYDVERFGIINTGNPAHADIFVVTGGVNE
CCCCCCCEEEEECCCCCCCCCEEEEEHHCCHHHHHHCCEEECCCCCCEEEEEEECCCCC
QNKVVLKNLYDQIPEPKVVVAVGICASSGGVFRDCYNISGGVDTIIPVDVYVPGCSVRPE
CCCHHHHHHHHHCCCCEEEEEEEEEECCCCEEEEEECCCCCCCEEEEEEEEECCCCCCHH
ALIEGIVKALGVLEEKRAALEAKK
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]