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The map label for this gene is mrpA [H]

Identifier: 124486436

GI number: 124486436

Start: 1650073

End: 1652064

Strand: Reverse

Name: mrpA [H]

Synonym: Mlab_1624

Alternate gene names: 124486436

Gene position: 1652064-1650073 (Counterclockwise)

Preceding gene: 124486437

Following gene: 124486435

Centisome position: 91.53

GC content: 51.71

Gene sequence:

>1992_bases
ATGATTAATAATGATGAATGCCCATACTTTCAACAAGTGATATCAATGTACCCGCCGGAACTCATTGCCATACTGATATT
GCTGCTCGTTCCTGTTTGCGCCGCTGCACTCTTAGCGGTCTTCAAACCGGATCTTGTCCGCAATATCCTCGTAGGCATAA
CTTCGGTCATTATTATCGGGACGTCCATCTTCCTCGCTTGGAAAGCATTCCTTGCTCCCGTATCTGTGACCCTTGAGAAT
ACCGAATGGATCGGCATTCTGTTATTCATCGTCGACCTGGCAATTGCCCTCATAATTCTGGGACTGTCCATAAAATATCG
GAAAATACTTCCGCTCATTCTTTCCGGGATCCAGCTCATCCTTATCGTGATCCTCGAACTCTTCGGTGTTACCGGACCCC
ATGCAATAAAAACGTTCACGGTCAGCAGTCTTTCCGCGATCATGGTTCTTATCATCGGAATAATCGGAACGCTGATCTGC
GTCTATGCTCTTGGCTACATGAAGGATTATCATGAACACCAGATCGGTGTTCCGGTGAGGAAACGCTACTTCTTTGCCGT
GATGTTCATCTTCCTCTCGGCAATGTTCGGGCTGGTTCTCTCGAACAATCTGATGTGGGTTTTGTGCGCCTGGGAAATAA
CCACGCTCTGCTCCTTCCTTCTGATCGGGTACTCCCGCACCCCCGAAGCAGTGAAAAATGCGTTCCGTGCCGTGTGGATG
AACCTGATCGGCGGGATATGCTTTACCCTGGCGATATTCTTCCTGCTTCGCATCAATTCGCCGGTAAGTCTTCTCTCGGT
AAACAACCTGCTCAACTTCCCGAACGTCTCTCTTCTCACGATACCTCTGGCATTGATCGCCGTCGCGGGATTGACCAAGG
CAGCTCAGATGCCGTTTTCCACCTGGCTTACGGGGGCAATGGTCGCTCCGACACCGGTCTCCGCCCTGCTCCACTCAAGT
ACCATGGTCAAGGCCGGCGTCTACCTTCTGGTCCTTTTCGCCCCGCTCTTCTCGCAGACCTGGGTTGGTATCTTCCTTGC
TTTGATCGGGGCATTCACCTTCCTTGTGACCTCGGCTCTGGCAATCTCGCAGAGTAATGCGAAGAAAGTTCTTGCCTACT
CCACGATCGCCAATCTCGGTCTGATCACCGCATGTGCCGGTATAGGCACGGCAGAGGCTATCGAAGCGGCTATCCTTTTG
ATCATCTTCCATGCGGTCGCCAAGGCGCTGCTCTTCCTTTGCGTCGGCGCAGTCGAACACAAGATCGGCAGCCGGGACAT
CGAGGATATGGACGGACTTCTGGTCCGTCTCCCGCTCTTGGCAACGATGATGGTCATTGGTATCTGCGGCATGTATCTTG
CGCCGTTTGGTATGCTCATTTCCAAGTGGATAGCTCTGGAAGCCTTCATCACCGCTCCGTTTGGATGCGTCTTCGCATCT
CTTCTTGCCTTTGGAAGTGCGTTTACCATATTCTTCTGGACCAAATGGCTCGGAAAACTGTTCATGAGAATGAACAGACC
GCCGGCAGAAAAGATCACCCTGCACTTCTCCGAAAAAATTTCGGTCATCGTCATGGGGATTCTCGTGGTTGCCTGCTGTC
TCGGGTTCCCGGCGATCATCTCCGGCGTGATCATTCCGTATCTCGACAGGATTCACCCGTACTTCTATCAGCCGAATAAC
GGATTATTCTTCTTCGATACCCTGGTCATGCTTGGTCTCATGGCGGCAATTCTCGTCTTCCTTGTGATCGGGGCTTTCCT
CGGATCCAAAGAAGGCAGACCCATTCCGCCTTATCTTGGCGGCCGTGCGGTCGATACGGAAGGCAGGTTCCTCGGCTCCC
TTGGCGTCTACAAAGAGGCGAAGGCCTCGAACTATTATCTCGAAGAATACTGCGGTGAGAACGTGCTTCTCAAACCCTCC
TGGGTTATCTCGGGAATCCTTCTCATCGTGATGCTTGTTCTCGGATTTATGGGGGTGATGATATGGATATAA

Upstream 100 bases:

>100_bases
CGAATATTTTGGAGGTATTCTTGAATATTTCCTCATGTTTTTCTGTATCGGGGCCTAGTATCCGCACCGAAAATGTTGAA
TCATCCCCGGGATGATTAGA

Downstream 100 bases:

>100_bases
TGTTTCTTGTAGGGGCGGTGGCCTTTCTGATACTTGCCCCGATCTTCGGCGGCCTTCTTACCGGATGCGACCGGGTACTG
ACTGCCCGTTTCCAGTCAAG

Product: ech hydrogenase subunit A

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 663; Mature: 663

Protein sequence:

>663_residues
MINNDECPYFQQVISMYPPELIAILILLLVPVCAAALLAVFKPDLVRNILVGITSVIIIGTSIFLAWKAFLAPVSVTLEN
TEWIGILLFIVDLAIALIILGLSIKYRKILPLILSGIQLILIVILELFGVTGPHAIKTFTVSSLSAIMVLIIGIIGTLIC
VYALGYMKDYHEHQIGVPVRKRYFFAVMFIFLSAMFGLVLSNNLMWVLCAWEITTLCSFLLIGYSRTPEAVKNAFRAVWM
NLIGGICFTLAIFFLLRINSPVSLLSVNNLLNFPNVSLLTIPLALIAVAGLTKAAQMPFSTWLTGAMVAPTPVSALLHSS
TMVKAGVYLLVLFAPLFSQTWVGIFLALIGAFTFLVTSALAISQSNAKKVLAYSTIANLGLITACAGIGTAEAIEAAILL
IIFHAVAKALLFLCVGAVEHKIGSRDIEDMDGLLVRLPLLATMMVIGICGMYLAPFGMLISKWIALEAFITAPFGCVFAS
LLAFGSAFTIFFWTKWLGKLFMRMNRPPAEKITLHFSEKISVIVMGILVVACCLGFPAIISGVIIPYLDRIHPYFYQPNN
GLFFFDTLVMLGLMAAILVFLVIGAFLGSKEGRPIPPYLGGRAVDTEGRFLGSLGVYKEAKASNYYLEEYCGENVLLKPS
WVISGILLIVMLVLGFMGVMIWI

Sequences:

>Translated_663_residues
MINNDECPYFQQVISMYPPELIAILILLLVPVCAAALLAVFKPDLVRNILVGITSVIIIGTSIFLAWKAFLAPVSVTLEN
TEWIGILLFIVDLAIALIILGLSIKYRKILPLILSGIQLILIVILELFGVTGPHAIKTFTVSSLSAIMVLIIGIIGTLIC
VYALGYMKDYHEHQIGVPVRKRYFFAVMFIFLSAMFGLVLSNNLMWVLCAWEITTLCSFLLIGYSRTPEAVKNAFRAVWM
NLIGGICFTLAIFFLLRINSPVSLLSVNNLLNFPNVSLLTIPLALIAVAGLTKAAQMPFSTWLTGAMVAPTPVSALLHSS
TMVKAGVYLLVLFAPLFSQTWVGIFLALIGAFTFLVTSALAISQSNAKKVLAYSTIANLGLITACAGIGTAEAIEAAILL
IIFHAVAKALLFLCVGAVEHKIGSRDIEDMDGLLVRLPLLATMMVIGICGMYLAPFGMLISKWIALEAFITAPFGCVFAS
LLAFGSAFTIFFWTKWLGKLFMRMNRPPAEKITLHFSEKISVIVMGILVVACCLGFPAIISGVIIPYLDRIHPYFYQPNN
GLFFFDTLVMLGLMAAILVFLVIGAFLGSKEGRPIPPYLGGRAVDTEGRFLGSLGVYKEAKASNYYLEEYCGENVLLKPS
WVISGILLIVMLVLGFMGVMIWI
>Mature_663_residues
MINNDECPYFQQVISMYPPELIAILILLLVPVCAAALLAVFKPDLVRNILVGITSVIIIGTSIFLAWKAFLAPVSVTLEN
TEWIGILLFIVDLAIALIILGLSIKYRKILPLILSGIQLILIVILELFGVTGPHAIKTFTVSSLSAIMVLIIGIIGTLIC
VYALGYMKDYHEHQIGVPVRKRYFFAVMFIFLSAMFGLVLSNNLMWVLCAWEITTLCSFLLIGYSRTPEAVKNAFRAVWM
NLIGGICFTLAIFFLLRINSPVSLLSVNNLLNFPNVSLLTIPLALIAVAGLTKAAQMPFSTWLTGAMVAPTPVSALLHSS
TMVKAGVYLLVLFAPLFSQTWVGIFLALIGAFTFLVTSALAISQSNAKKVLAYSTIANLGLITACAGIGTAEAIEAAILL
IIFHAVAKALLFLCVGAVEHKIGSRDIEDMDGLLVRLPLLATMMVIGICGMYLAPFGMLISKWIALEAFITAPFGCVFAS
LLAFGSAFTIFFWTKWLGKLFMRMNRPPAEKITLHFSEKISVIVMGILVVACCLGFPAIISGVIIPYLDRIHPYFYQPNN
GLFFFDTLVMLGLMAAILVFLVIGAFLGSKEGRPIPPYLGGRAVDTEGRFLGSLGVYKEAKASNYYLEEYCGENVLLKPS
WVISGILLIVMLVLGFMGVMIWI

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=284, Percent_Identity=39.4366197183099, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI1788614, Length=324, Percent_Identity=33.9506172839506, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788829, Length=396, Percent_Identity=30.050505050505, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788831, Length=348, Percent_Identity=29.5977011494253, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1788827, Length=352, Percent_Identity=27.8409090909091, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI2367154, Length=459, Percent_Identity=25.7080610021786, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788613, Length=373, Percent_Identity=23.8605898123324, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 72320; Mature: 72320

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINNDECPYFQQVISMYPPELIAILILLLVPVCAAALLAVFKPDLVRNILVGITSVIIIG
CCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
TSIFLAWKAFLAPVSVTLENTEWIGILLFIVDLAIALIILGLSIKYRKILPLILSGIQLI
HHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LIVILELFGVTGPHAIKTFTVSSLSAIMVLIIGIIGTLICVYALGYMKDYHEHQIGVPVR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KRYFFAVMFIFLSAMFGLVLSNNLMWVLCAWEITTLCSFLLIGYSRTPEAVKNAFRAVWM
HHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
NLIGGICFTLAIFFLLRINSPVSLLSVNNLLNFPNVSLLTIPLALIAVAGLTKAAQMPFS
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
TWLTGAMVAPTPVSALLHSSTMVKAGVYLLVLFAPLFSQTWVGIFLALIGAFTFLVTSAL
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AISQSNAKKVLAYSTIANLGLITACAGIGTAEAIEAAILLIIFHAVAKALLFLCVGAVEH
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIGSRDIEDMDGLLVRLPLLATMMVIGICGMYLAPFGMLISKWIALEAFITAPFGCVFAS
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LLAFGSAFTIFFWTKWLGKLFMRMNRPPAEKITLHFSEKISVIVMGILVVACCLGFPAII
HHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHEEEEHHHHHHHHHHHHHHHHHHHCHHHHH
SGVIIPYLDRIHPYFYQPNNGLFFFDTLVMLGLMAAILVFLVIGAFLGSKEGRPIPPYLG
HHHHHHHHHHCCCCEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GRAVDTEGRFLGSLGVYKEAKASNYYLEEYCGENVLLKPSWVISGILLIVMLVLGFMGVM
CEEECCCCCHHHCCCCHHHHHCCCCHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHH
IWI
HCC
>Mature Secondary Structure
MINNDECPYFQQVISMYPPELIAILILLLVPVCAAALLAVFKPDLVRNILVGITSVIIIG
CCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
TSIFLAWKAFLAPVSVTLENTEWIGILLFIVDLAIALIILGLSIKYRKILPLILSGIQLI
HHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LIVILELFGVTGPHAIKTFTVSSLSAIMVLIIGIIGTLICVYALGYMKDYHEHQIGVPVR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KRYFFAVMFIFLSAMFGLVLSNNLMWVLCAWEITTLCSFLLIGYSRTPEAVKNAFRAVWM
HHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
NLIGGICFTLAIFFLLRINSPVSLLSVNNLLNFPNVSLLTIPLALIAVAGLTKAAQMPFS
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
TWLTGAMVAPTPVSALLHSSTMVKAGVYLLVLFAPLFSQTWVGIFLALIGAFTFLVTSAL
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AISQSNAKKVLAYSTIANLGLITACAGIGTAEAIEAAILLIIFHAVAKALLFLCVGAVEH
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIGSRDIEDMDGLLVRLPLLATMMVIGICGMYLAPFGMLISKWIALEAFITAPFGCVFAS
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LLAFGSAFTIFFWTKWLGKLFMRMNRPPAEKITLHFSEKISVIVMGILVVACCLGFPAII
HHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHEEEEHHHHHHHHHHHHHHHHHHHCHHHHH
SGVIIPYLDRIHPYFYQPNNGLFFFDTLVMLGLMAAILVFLVIGAFLGSKEGRPIPPYLG
HHHHHHHHHHCCCCEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GRAVDTEGRFLGSLGVYKEAKASNYYLEEYCGENVLLKPSWVISGILLIVMLVLGFMGVM
CEEECCCCCHHHCCCCHHHHHCCCCHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHH
IWI
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194 [H]