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The map label for this gene is ogt [H]

Identifier: 124485923

GI number: 124485923

Start: 1115036

End: 1115482

Strand: Reverse

Name: ogt [H]

Synonym: Mlab_1103

Alternate gene names: 124485923

Gene position: 1115482-1115036 (Counterclockwise)

Preceding gene: 124485924

Following gene: 124485922

Centisome position: 61.8

GC content: 53.24

Gene sequence:

>447_bases
ATGCGTCAGGGCGCCTGTAGTTTCGGACTCTGGAAGGTCGCTGTCGATTATGACGATATGGTAGTCCACCGGGTCAGGTT
CATGAGAACGGCACCGGACGGGCCGGTTCCGATAGAGTTCACCCGATTTCTTGCGGGAAAAGGGACCGGCTTTGCTCCGC
TGACCTCTGCGGCTCTGGGTGGTGATGGGACATATGCAGAAATCTATCGTGCCGTATCAAGGATCCCATACGGAGAAACG
CAAAGTTACGGCGAGATTGCCAAGGCAGCAGAAACACACCCGCGGGTGGTCGGAAATGCTATGGCACGAAACCCCACACC
TTTAATTGTCCCGTGCCATCGGGTCATCTCATCCGACGGAAGTCTTGGCGGTTTCTCACCGGACCTTGAAATCAAAAAAT
CTCTTTTACAGCTCGAAAAAAGCTCGTTACGACGGCATATTTCGTAA

Upstream 100 bases:

>100_bases
GAAGGCGAGATTTTCGGCGGTTTATCTCCCTGGATGCAGGATGAACTTATGGAATGCGATGTGGACACGGTAAAAAGTAA
GCTTGAAAAGTGGGGACGTG

Downstream 100 bases:

>100_bases
TTATTTTGCATCACCTGCTGGTTTAATCCATGTGAACAGCAAATAATAAGGGACTGAAATGGACCTTCGTCTGCTCATAT
TTCCCCTCCTTGTCTTCCTT

Product: hypothetical protein

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MRQGACSFGLWKVAVDYDDMVVHRVRFMRTAPDGPVPIEFTRFLAGKGTGFAPLTSAALGGDGTYAEIYRAVSRIPYGET
QSYGEIAKAAETHPRVVGNAMARNPTPLIVPCHRVISSDGSLGGFSPDLEIKKSLLQLEKSSLRRHIS

Sequences:

>Translated_148_residues
MRQGACSFGLWKVAVDYDDMVVHRVRFMRTAPDGPVPIEFTRFLAGKGTGFAPLTSAALGGDGTYAEIYRAVSRIPYGET
QSYGEIAKAAETHPRVVGNAMARNPTPLIVPCHRVISSDGSLGGFSPDLEIKKSLLQLEKSSLRRHIS
>Mature_148_residues
MRQGACSFGLWKVAVDYDDMVVHRVRFMRTAPDGPVPIEFTRFLAGKGTGFAPLTSAALGGDGTYAEIYRAVSRIPYGET
QSYGEIAKAAETHPRVVGNAMARNPTPLIVPCHRVISSDGSLGGFSPDLEIKKSLLQLEKSSLRRHIS

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Caenorhabditis elegans, GI115533070, Length=92, Percent_Identity=42.3913043478261, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115533068, Length=93, Percent_Identity=41.9354838709677, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1 [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 16065; Mature: 16065

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQGACSFGLWKVAVDYDDMVVHRVRFMRTAPDGPVPIEFTRFLAGKGTGFAPLTSAALG
CCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHCC
GDGTYAEIYRAVSRIPYGETQSYGEIAKAAETHPRVVGNAMARNPTPLIVPCHRVISSDG
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCEEEECHHHHCCCC
SLGGFSPDLEIKKSLLQLEKSSLRRHIS
CCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRQGACSFGLWKVAVDYDDMVVHRVRFMRTAPDGPVPIEFTRFLAGKGTGFAPLTSAALG
CCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHCC
GDGTYAEIYRAVSRIPYGETQSYGEIAKAAETHPRVVGNAMARNPTPLIVPCHRVISSDG
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCEEEECHHHHCCCC
SLGGFSPDLEIKKSLLQLEKSSLRRHIS
CCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]