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The map label for this gene is ilvE [H]

Identifier: 124485301

GI number: 124485301

Start: 451955

End: 452866

Strand: Reverse

Name: ilvE [H]

Synonym: Mlab_0476

Alternate gene names: 124485301

Gene position: 452866-451955 (Counterclockwise)

Preceding gene: 124485305

Following gene: 124485300

Centisome position: 25.09

GC content: 51.32

Gene sequence:

>912_bases
ATGCATAGACCAAAATCCTATATCATACCACATCACATATTATTTATCATGTTAGTTTACATAAACGGGAAATTTGTGCC
TGAAGAACAGGCAACCGTGTCAATTTTCGACCACGGATTCCTTTACGGAGACGGTGTGTTTGAAGGAATCAGAGCATACA
ATGGTCGTGTGTTCCGCCTCAAAGAACACGTTGACCGTCTCTTCGACTCGGCTAAAGCCTTAGATCTTGATCCCGGGATC
ACCCGCGCCGAGATGGCCGAGATCATCAAAGAGACTCTGCGCCAGAACAACCTGAAGGATGCCTACATCCGTCCGATCAT
TACGCGTGGTGTCGGAACTATGGGACTCGACCCGCGTCACTGCGCAAACCCCTCGATCATCTGTGCTGCTTCAGCCTGGG
GAGCAATGTACGGCGACCTCTATGAAACCGGTCTTACCGCGGTGACGGTCTGTGTGAGAAGAAACCCGCCGGACACTCTC
CCGCCGAACATCAAATCCCTCAACTACCTGAACAACATTCTTGGTAAGATCGAGGCAAACTACAAGGGCGGCGACGAAGC
CATCTTCCTTGATCGAACCGGAAAACTTGCAGAAGGTTCCGGCGACAACATCTATCTCATAAAAGACGGCGTTCTCTACA
CTCCTCCGACCATCAACAACCTCAAAGGCATCACCCGCCTTGTCCTTCTCGACATCTGCGCGAAAATCGGGATCCGCTAC
GAGATCTGCGAGCTCGGACTCTTTGATCTTTACACCGCCGACGAAGTCATGGTCACAGGAACGGCCGCAGAAATCTGCGC
AATCACCAAAATAGACGGCAGAACGATCGGAACCGGCGTTCCAGGCCCAGTCTACAAAAAACTTCTTGCATCATTCCAGA
CGGAGACTCAGAACTCCGGCGATGCATACTGA

Upstream 100 bases:

>100_bases
TATTTGGCGGGGCCGGGATATCCTGAAAAATCACCCTGCATGAACCCTCGTTTTTTCCGACACTATCACCGAAATAAACG
CATGAACGCCGGATTTTATC

Downstream 100 bases:

>100_bases
TCCTGAAATCAAAAGGGATCAATCAAAATCTTTTTATAAATCAGCGTAGACTATTATAGGCAGTTTGAGCTGATATAGTG
TAGAGGCCAATCATGCCGGC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MHRPKSYIIPHHILFIMLVYINGKFVPEEQATVSIFDHGFLYGDGVFEGIRAYNGRVFRLKEHVDRLFDSAKALDLDPGI
TRAEMAEIIKETLRQNNLKDAYIRPIITRGVGTMGLDPRHCANPSIICAASAWGAMYGDLYETGLTAVTVCVRRNPPDTL
PPNIKSLNYLNNILGKIEANYKGGDEAIFLDRTGKLAEGSGDNIYLIKDGVLYTPPTINNLKGITRLVLLDICAKIGIRY
EICELGLFDLYTADEVMVTGTAAEICAITKIDGRTIGTGVPGPVYKKLLASFQTETQNSGDAY

Sequences:

>Translated_303_residues
MHRPKSYIIPHHILFIMLVYINGKFVPEEQATVSIFDHGFLYGDGVFEGIRAYNGRVFRLKEHVDRLFDSAKALDLDPGI
TRAEMAEIIKETLRQNNLKDAYIRPIITRGVGTMGLDPRHCANPSIICAASAWGAMYGDLYETGLTAVTVCVRRNPPDTL
PPNIKSLNYLNNILGKIEANYKGGDEAIFLDRTGKLAEGSGDNIYLIKDGVLYTPPTINNLKGITRLVLLDICAKIGIRY
EICELGLFDLYTADEVMVTGTAAEICAITKIDGRTIGTGVPGPVYKKLLASFQTETQNSGDAY
>Mature_303_residues
MHRPKSYIIPHHILFIMLVYINGKFVPEEQATVSIFDHGFLYGDGVFEGIRAYNGRVFRLKEHVDRLFDSAKALDLDPGI
TRAEMAEIIKETLRQNNLKDAYIRPIITRGVGTMGLDPRHCANPSIICAASAWGAMYGDLYETGLTAVTVCVRRNPPDTL
PPNIKSLNYLNNILGKIEANYKGGDEAIFLDRTGKLAEGSGDNIYLIKDGVLYTPPTINNLKGITRLVLLDICAKIGIRY
EICELGLFDLYTADEVMVTGTAAEICAITKIDGRTIGTGVPGPVYKKLLASFQTETQNSGDAY

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=281, Percent_Identity=39.8576512455516, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1787338, Length=248, Percent_Identity=27.0161290322581, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 33408; Mature: 33408

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRPKSYIIPHHILFIMLVYINGKFVPEEQATVSIFDHGFLYGDGVFEGIRAYNGRVFRL
CCCCCCCCCHHHHHHHHHHHHCCEECCCCCCEEEEEECCEEECCHHHHHHHHHCCEEEEH
KEHVDRLFDSAKALDLDPGITRAEMAEIIKETLRQNNLKDAYIRPIITRGVGTMGLDPRH
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHH
CANPSIICAASAWGAMYGDLYETGLTAVTVCVRRNPPDTLPPNIKSLNYLNNILGKIEAN
CCCCCEEEEEHHHHHHHHHHHHHCHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHEECC
YKGGDEAIFLDRTGKLAEGSGDNIYLIKDGVLYTPPTINNLKGITRLVLLDICAKIGIRY
CCCCCCEEEEECCCCCCCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHCCEE
EICELGLFDLYTADEVMVTGTAAEICAITKIDGRTIGTGVPGPVYKKLLASFQTETQNSG
EHHHCCHHHEECCCCEEEECCHHHEEEEEEECCEEECCCCCCHHHHHHHHHHHHCCCCCC
DAY
CCC
>Mature Secondary Structure
MHRPKSYIIPHHILFIMLVYINGKFVPEEQATVSIFDHGFLYGDGVFEGIRAYNGRVFRL
CCCCCCCCCHHHHHHHHHHHHCCEECCCCCCEEEEEECCEEECCHHHHHHHHHCCEEEEH
KEHVDRLFDSAKALDLDPGITRAEMAEIIKETLRQNNLKDAYIRPIITRGVGTMGLDPRH
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHH
CANPSIICAASAWGAMYGDLYETGLTAVTVCVRRNPPDTLPPNIKSLNYLNNILGKIEAN
CCCCCEEEEEHHHHHHHHHHHHHCHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHEECC
YKGGDEAIFLDRTGKLAEGSGDNIYLIKDGVLYTPPTINNLKGITRLVLLDICAKIGIRY
CCCCCCEEEEECCCCCCCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHCCEE
EICELGLFDLYTADEVMVTGTAAEICAITKIDGRTIGTGVPGPVYKKLLASFQTETQNSG
EHHHCCHHHEECCCCEEEECCHHHEEEEEEECCEEECCCCCCHHHHHHHHHHHHCCCCCC
DAY
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]