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The map label for this gene is nifU [H]

Identifier: 124485092

GI number: 124485092

Start: 247547

End: 247978

Strand: Reverse

Name: nifU [H]

Synonym: Mlab_0265

Alternate gene names: 124485092

Gene position: 247978-247547 (Counterclockwise)

Preceding gene: 124485093

Following gene: 124485091

Centisome position: 13.74

GC content: 50.0

Gene sequence:

>432_bases
ATGTACAGTGAAAAAGTAATGGATCACTTCACTAACCCGCGAAACGTGGGTATGATCGAGGACCCAAGCGGAGTCGGCGA
AGAGGGGAACCCCTCCTGCGGCGACATTATGAAAATGTATCTGAAAGTCGAAAACGACATCATCGTGGATGCAAAATTCC
AGACATTCGGCTGTGGTGCTGCGATTGCCTCCAGCAGCATGGCTACCGAGATGATCAAAGGAAAAACCCTGGAAGAAGCA
TGGACCCTTTCAAACAAAGCAGTGGCAGAGGCGCTGGACGGATTACCGCCGAACAAAATGCATTGTTCGGTCCTAGCCGA
GGAAACAATCCACAAAGCAATCAACGATTATCTGGTAAAGTCCGGCAGAGAACCGTGGGGAGATGAAGAATGCAGCTCCT
GCGGACATGACTGTGATACGTGTGAACTCTGA

Upstream 100 bases:

>100_bases
CGGACGACGGAAGACGATGTGAAATATGTGATCACCGCACTCACAGAAACGGTGGCAAAACTCAGGGCAATGTCCCCCCT
TACTCCAAAGGAGCTGAGAA

Downstream 100 bases:

>100_bases
GATGAACCTTCCGCGTGCAGTAATCTTCGACATGGATAACACGCTGCACGATCTGCGTCGGGCACGCCATTGTGCTGCCG
ATGCTTTAATGGCATACTGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MYSEKVMDHFTNPRNVGMIEDPSGVGEEGNPSCGDIMKMYLKVENDIIVDAKFQTFGCGAAIASSSMATEMIKGKTLEEA
WTLSNKAVAEALDGLPPNKMHCSVLAEETIHKAINDYLVKSGREPWGDEECSSCGHDCDTCEL

Sequences:

>Translated_143_residues
MYSEKVMDHFTNPRNVGMIEDPSGVGEEGNPSCGDIMKMYLKVENDIIVDAKFQTFGCGAAIASSSMATEMIKGKTLEEA
WTLSNKAVAEALDGLPPNKMHCSVLAEETIHKAINDYLVKSGREPWGDEECSSCGHDCDTCEL
>Mature_143_residues
MYSEKVMDHFTNPRNVGMIEDPSGVGEEGNPSCGDIMKMYLKVENDIIVDAKFQTFGCGAAIASSSMATEMIKGKTLEEA
WTLSNKAVAEALDGLPPNKMHCSVLAEETIHKAINDYLVKSGREPWGDEECSSCGHDCDTCEL

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=133, Percent_Identity=53.3834586466165, Blast_Score=135, Evalue=9e-33,
Organism=Homo sapiens, GI24307953, Length=128, Percent_Identity=53.90625, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI1788878, Length=127, Percent_Identity=55.1181102362205, Blast_Score=134, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17543474, Length=127, Percent_Identity=51.9685039370079, Blast_Score=129, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6324800, Length=130, Percent_Identity=48.4615384615385, Blast_Score=123, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325122, Length=128, Percent_Identity=49.21875, Blast_Score=121, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21355597, Length=128, Percent_Identity=53.125, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002871 [H]

Pfam domain/function: PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 15569; Mature: 15569

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
10.5 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
10.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSEKVMDHFTNPRNVGMIEDPSGVGEEGNPSCGDIMKMYLKVENDIIVDAKFQTFGCGA
CCCHHHHHHCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCHHCCHH
AIASSSMATEMIKGKTLEEAWTLSNKAVAEALDGLPPNKMHCSVLAEETIHKAINDYLVK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SGREPWGDEECSSCGHDCDTCEL
CCCCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MYSEKVMDHFTNPRNVGMIEDPSGVGEEGNPSCGDIMKMYLKVENDIIVDAKFQTFGCGA
CCCHHHHHHCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCHHCCHH
AIASSSMATEMIKGKTLEEAWTLSNKAVAEALDGLPPNKMHCSVLAEETIHKAINDYLVK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SGREPWGDEECSSCGHDCDTCEL
CCCCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]