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The map label for this gene is iscS [H]

Identifier: 124485093

GI number: 124485093

Start: 247968

End: 249149

Strand: Reverse

Name: iscS [H]

Synonym: Mlab_0266

Alternate gene names: 124485093

Gene position: 249149-247968 (Counterclockwise)

Preceding gene: 124485097

Following gene: 124485092

Centisome position: 13.8

GC content: 48.73

Gene sequence:

>1182_bases
ATGGGTAAAAAAATCATCTATATGGACCATGCTGCTACGACTTACACCGCAAAAGAGGTCGTAGAGGCAATGCTACCTTA
TTTCTCCGAACAATTCGGCAATCCCTCTTCAGTTTATTCTATTGGGCAGTCCAACAAGAGTGTCATTGATCTAGCCCGAA
AACAGGTGGCATCAGCGATCAATGCTCAGCCTGATGAAATTTTCTTTACCAACGGCGGGACCGAATCGGACAACTGGGCG
CTGAAAGGTGTGGCATTTGCCAATGAAAGAAAAGGAAAACATATCATCACAACAGCGATTGAGCATCATGCAATTCTGCA
TTCCTGTGAATGGCTCGCATCACGCGGATTTGAAATAACCTATCTTCCGGTAGACAAATACGGTATGGTTTCTCCAGAGG
CGGTCGAAAAAGCGATTCGCCCGGACACGATTCTCATCTCGGTTATGTATGCAAACAACGAGGTCGGAACGATTCAACCG
ATTGCCGAGATCGGAAAGATCGCGAGAGATCATGGAATATATTTCCACACCGACGCGGTTCAGGCAGTTGGCCATGTTCC
AATAGATGTGGTTGCCGAAAATATTGATCTTCTCTCGCTCTCCGGTCACAAATTCTACGGACCGAAAGGTACCGGAGCCC
TTTACATCCGGAAAGGAACGCGGATCCAGAACTTTATCCACGGCGGGGCTCAGGAGAAAAAACGCCGGGCAGGTACTGAA
AATGTTCCCGGCATCGTGGGCCTCGGCGCTGCGGTAGAACGGGCAATCAAAATGATGCCGGTGGAGACGAAACGACTTGC
ATCTCTGAGTGATGAACTTACTCGTGAACTTCTCAAAATCCCGGCAACACACTTAAACGGCCATCCGACAAAACGTCTTC
CAAACAACACGAACATTATCTTCGAGTATATTGAAGGAGAATCAATTCTTCTGTTTCTGAACATGAAGGGCATCTGTGCT
TCGACCGGAAGTGCCTGTAACTCGGCATCCCTTGAACCATCTCATGTCTTAACCGCCATGGGAATATCCCACGAGATCGC
ACACGGGTCCATCCGGCTTACCGTCGGCGAACGGACGACGGAAGACGATGTGAAATATGTGATCACCGCACTCACAGAAA
CGGTGGCAAAACTCAGGGCAATGTCCCCCCTTACTCCAAAGGAGCTGAGAAATGTACAGTGA

Upstream 100 bases:

>100_bases
AGCATTCGCTTTCACACGACATCCAACATGTTCCTGTCACGAATATTTAGATATAATAACGGCGAAATAATATAGTAAGG
AAATTCACGGCGTGAACACC

Downstream 100 bases:

>100_bases
AAAAGTAATGGATCACTTCACTAACCCGCGAAACGTGGGTATGATCGAGGACCCAAGCGGAGTCGGCGAAGAGGGGAACC
CCTCCTGCGGCGACATTATG

Product: hypothetical protein

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MGKKIIYMDHAATTYTAKEVVEAMLPYFSEQFGNPSSVYSIGQSNKSVIDLARKQVASAINAQPDEIFFTNGGTESDNWA
LKGVAFANERKGKHIITTAIEHHAILHSCEWLASRGFEITYLPVDKYGMVSPEAVEKAIRPDTILISVMYANNEVGTIQP
IAEIGKIARDHGIYFHTDAVQAVGHVPIDVVAENIDLLSLSGHKFYGPKGTGALYIRKGTRIQNFIHGGAQEKKRRAGTE
NVPGIVGLGAAVERAIKMMPVETKRLASLSDELTRELLKIPATHLNGHPTKRLPNNTNIIFEYIEGESILLFLNMKGICA
STGSACNSASLEPSHVLTAMGISHEIAHGSIRLTVGERTTEDDVKYVITALTETVAKLRAMSPLTPKELRNVQ

Sequences:

>Translated_393_residues
MGKKIIYMDHAATTYTAKEVVEAMLPYFSEQFGNPSSVYSIGQSNKSVIDLARKQVASAINAQPDEIFFTNGGTESDNWA
LKGVAFANERKGKHIITTAIEHHAILHSCEWLASRGFEITYLPVDKYGMVSPEAVEKAIRPDTILISVMYANNEVGTIQP
IAEIGKIARDHGIYFHTDAVQAVGHVPIDVVAENIDLLSLSGHKFYGPKGTGALYIRKGTRIQNFIHGGAQEKKRRAGTE
NVPGIVGLGAAVERAIKMMPVETKRLASLSDELTRELLKIPATHLNGHPTKRLPNNTNIIFEYIEGESILLFLNMKGICA
STGSACNSASLEPSHVLTAMGISHEIAHGSIRLTVGERTTEDDVKYVITALTETVAKLRAMSPLTPKELRNVQ
>Mature_392_residues
GKKIIYMDHAATTYTAKEVVEAMLPYFSEQFGNPSSVYSIGQSNKSVIDLARKQVASAINAQPDEIFFTNGGTESDNWAL
KGVAFANERKGKHIITTAIEHHAILHSCEWLASRGFEITYLPVDKYGMVSPEAVEKAIRPDTILISVMYANNEVGTIQPI
AEIGKIARDHGIYFHTDAVQAVGHVPIDVVAENIDLLSLSGHKFYGPKGTGALYIRKGTRIQNFIHGGAQEKKRRAGTEN
VPGIVGLGAAVERAIKMMPVETKRLASLSDELTRELLKIPATHLNGHPTKRLPNNTNIIFEYIEGESILLFLNMKGICAS
TGSACNSASLEPSHVLTAMGISHEIAHGSIRLTVGERTTEDDVKYVITALTETVAKLRAMSPLTPKELRNVQ

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=383, Percent_Identity=48.5639686684073, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=35.2657004830918, Blast_Score=217, Evalue=2e-56,
Organism=Escherichia coli, GI48994898, Length=384, Percent_Identity=45.0520833333333, Blast_Score=342, Evalue=4e-95,
Organism=Escherichia coli, GI1787970, Length=351, Percent_Identity=28.2051282051282, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1789175, Length=400, Percent_Identity=26.75, Blast_Score=115, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25143064, Length=384, Percent_Identity=46.6145833333333, Blast_Score=334, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI17533177, Length=329, Percent_Identity=31.306990881459, Blast_Score=167, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6319831, Length=387, Percent_Identity=46.5116279069767, Blast_Score=343, Evalue=3e-95,
Organism=Drosophila melanogaster, GI20129463, Length=383, Percent_Identity=45.6919060052219, Blast_Score=331, Evalue=5e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42887; Mature: 42755

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00595 AA_TRANSFER_CLASS_5 ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKIIYMDHAATTYTAKEVVEAMLPYFSEQFGNPSSVYSIGQSNKSVIDLARKQVASAI
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCHHHHHHHHHHHHHHH
NAQPDEIFFTNGGTESDNWALKGVAFANERKGKHIITTAIEHHAILHSCEWLASRGFEIT
CCCCCEEEEECCCCCCCCEEEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEE
YLPVDKYGMVSPEAVEKAIRPDTILISVMYANNEVGTIQPIAEIGKIARDHGIYFHTDAV
EEECCCCCCCCHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEHHH
QAVGHVPIDVVAENIDLLSLSGHKFYGPKGTGALYIRKGTRIQNFIHGGAQEKKRRAGTE
HHHCCCCHHHHHCCCCEEEECCCEEECCCCCCEEEEECCCHHHHHHHCCHHHHHHHCCCC
NVPGIVGLGAAVERAIKMMPVETKRLASLSDELTRELLKIPATHLNGHPTKRLPNNTNII
CCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCEE
FEYIEGESILLFLNMKGICASTGSACNSASLEPSHVLTAMGISHEIAHGSIRLTVGERTT
EEEECCCEEEEEEECCCEECCCCCCCCCCCCCHHHHHHHHCCCCEECCCEEEEEECCCCC
EDDVKYVITALTETVAKLRAMSPLTPKELRNVQ
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCC
>Mature Secondary Structure 
GKKIIYMDHAATTYTAKEVVEAMLPYFSEQFGNPSSVYSIGQSNKSVIDLARKQVASAI
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCHHHHHHHHHHHHHHH
NAQPDEIFFTNGGTESDNWALKGVAFANERKGKHIITTAIEHHAILHSCEWLASRGFEIT
CCCCCEEEEECCCCCCCCEEEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEE
YLPVDKYGMVSPEAVEKAIRPDTILISVMYANNEVGTIQPIAEIGKIARDHGIYFHTDAV
EEECCCCCCCCHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEHHH
QAVGHVPIDVVAENIDLLSLSGHKFYGPKGTGALYIRKGTRIQNFIHGGAQEKKRRAGTE
HHHCCCCHHHHHCCCCEEEECCCEEECCCCCCEEEEECCCHHHHHHHCCHHHHHHHCCCC
NVPGIVGLGAAVERAIKMMPVETKRLASLSDELTRELLKIPATHLNGHPTKRLPNNTNII
CCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCEE
FEYIEGESILLFLNMKGICASTGSACNSASLEPSHVLTAMGISHEIAHGSIRLTVGERTT
EEEECCCEEEEEEECCCEECCCCCCCCCCCCCHHHHHHHHCCCCEECCCEEEEEECCCCC
EDDVKYVITALTETVAKLRAMSPLTPKELRNVQ
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]