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The map label for this gene is panB1

Identifier: 124265976

GI number: 124265976

Start: 819147

End: 820031

Strand: Direct

Name: panB1

Synonym: Mpe_A0783

Alternate gene names: 124265976

Gene position: 819147-820031 (Clockwise)

Preceding gene: 124265975

Following gene: 124265989

Centisome position: 20.25

GC content: 67.12

Gene sequence:

>885_bases
ATGTCCGATGCCGCCGCTGCCCCGCCCGAGCGCAAGAAGCGCACCATCACCGAGATCCGCGACTCGAAGAAGAGCGGCGA
GAAGATGGTCTACACCTCGGTGCCGGACTACACCTCGGCCAAGTGGGCCGAGCTGGCCGGCGTCGACGTGGCCGTGGTCG
GCGACAGCCTGGCGATGGTCGCGCACGGGCACAGCAGCACCGTGCCGGCGACGATGGACATGATGGTGATGCACGCGCAG
GCGGTGCGGCGCGGCGCGCCGCGCACCTTCACGCTCGGCTGCATGCCCTACCAGAGCTACAACACGGTGGACCGCGCGCT
GCTCAACGCCACCCGCTTCATGCAGGACGGCGGCTGCGACGCGGTCAAGCCGCAGGGCGGCAGATCGCAGGCGCACATCC
TGAAGGCATTGGTCGACTCGGGCATCCCGACCGCCTCGCACATCGGGCTCACACCGCACACCATCGCGATGTTCGGCGGC
TTCAAGATCCAGGGCCGCACCGCCGAGGCGGCGATGAAGATCCTGGAGGACGCGTTCGCGATCCAGGACGCCGGCTGCTT
CATGCTGGAGTTCGAGGCGGTGCCGGCGAAGATCGCGACGCTGATCTCGAAGCAGCTCGAGATCCCCACCATCGGCATCG
GTGCCGGGGCCGGCTGCGATGGCCAGATCCTGCTGTCCTACGACCTGCTGGGCGTGTTCACCGACTTCAAGCCCAAGTTC
ACGAAGCGCTATGCCAACCTGACCGAGGTGGCGGTGCAGGGCCTGAAGGCTTACGTCGACGAGGTGAAGACCGGCCGCTT
CCCCGACGACGACCACAGCTATGGCGTCGACGACCGCGAGTACGAGCAGTTCATGAACCTGGTGGAGAAGCGGCGGCACG
TGTGA

Upstream 100 bases:

>100_bases
CTCGGCCGCCGACCGCCGCGCAGCGCGGCGTTTCCCCGCATTGGCGCCCTGTCGAACATTGATATGCTAGTGACATATCA
AACGCTCGAAGGAGACCGCC

Downstream 100 bases:

>100_bases
CGCTAGGGCCTGTTCACGCTATCGGAGGGCTCGCGCCGGCGCCATCCTCGAGCGCCTTCTTCTTCGCGCCGTCGAAGTCG
GTGCTTCAGCGCACCAGCGC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase 1; KPHMT 1

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSDAAAAPPERKKRTITEIRDSKKSGEKMVYTSVPDYTSAKWAELAGVDVAVVGDSLAMVAHGHSSTVPATMDMMVMHAQ
AVRRGAPRTFTLGCMPYQSYNTVDRALLNATRFMQDGGCDAVKPQGGRSQAHILKALVDSGIPTASHIGLTPHTIAMFGG
FKIQGRTAEAAMKILEDAFAIQDAGCFMLEFEAVPAKIATLISKQLEIPTIGIGAGAGCDGQILLSYDLLGVFTDFKPKF
TKRYANLTEVAVQGLKAYVDEVKTGRFPDDDHSYGVDDREYEQFMNLVEKRRHV

Sequences:

>Translated_294_residues
MSDAAAAPPERKKRTITEIRDSKKSGEKMVYTSVPDYTSAKWAELAGVDVAVVGDSLAMVAHGHSSTVPATMDMMVMHAQ
AVRRGAPRTFTLGCMPYQSYNTVDRALLNATRFMQDGGCDAVKPQGGRSQAHILKALVDSGIPTASHIGLTPHTIAMFGG
FKIQGRTAEAAMKILEDAFAIQDAGCFMLEFEAVPAKIATLISKQLEIPTIGIGAGAGCDGQILLSYDLLGVFTDFKPKF
TKRYANLTEVAVQGLKAYVDEVKTGRFPDDDHSYGVDDREYEQFMNLVEKRRHV
>Mature_293_residues
SDAAAAPPERKKRTITEIRDSKKSGEKMVYTSVPDYTSAKWAELAGVDVAVVGDSLAMVAHGHSSTVPATMDMMVMHAQA
VRRGAPRTFTLGCMPYQSYNTVDRALLNATRFMQDGGCDAVKPQGGRSQAHILKALVDSGIPTASHIGLTPHTIAMFGGF
KIQGRTAEAAMKILEDAFAIQDAGCFMLEFEAVPAKIATLISKQLEIPTIGIGAGAGCDGQILLSYDLLGVFTDFKPKFT
KRYANLTEVAVQGLKAYVDEVKTGRFPDDDHSYGVDDREYEQFMNLVEKRRHV

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=263, Percent_Identity=37.6425855513308, Blast_Score=192, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6319653, Length=289, Percent_Identity=33.5640138408304, Blast_Score=178, Evalue=1e-45,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB1_METPP (A2SDV6)

Other databases:

- EMBL:   CP000555
- RefSeq:   YP_001019980.1
- ProteinModelPortal:   A2SDV6
- SMR:   A2SDV6
- STRING:   A2SDV6
- GeneID:   4784171
- GenomeReviews:   CP000555_GR
- KEGG:   mpt:Mpe_A0783
- NMPDR:   fig|279263.3.peg.4102
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   PIAQKIT
- PhylomeDB:   A2SDV6
- ProtClustDB:   CLSK2471488
- BioCyc:   MPET420662:MPE_A0783-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 31928; Mature: 31797

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: ACT_SITE 192-192 BINDING 123-123

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAAAAPPERKKRTITEIRDSKKSGEKMVYTSVPDYTSAKWAELAGVDVAVVGDSLAMV
CCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCEEEEECCCEEEE
AHGHSSTVPATMDMMVMHAQAVRRGAPRTFTLGCMPYQSYNTVDRALLNATRFMQDGGCD
ECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCC
AVKPQGGRSQAHILKALVDSGIPTASHIGLTPHTIAMFGGFKIQGRTAEAAMKILEDAFA
CCCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCHHHHHCCCEEEECCCHHHHHHHHHHHHH
IQDAGCFMLEFEAVPAKIATLISKQLEIPTIGIGAGAGCDGQILLSYDLLGVFTDFKPKF
HHCCCEEEEEECHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEHHHHHHHCCCHHH
TKRYANLTEVAVQGLKAYVDEVKTGRFPDDDHSYGVDDREYEQFMNLVEKRRHV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDAAAAPPERKKRTITEIRDSKKSGEKMVYTSVPDYTSAKWAELAGVDVAVVGDSLAMV
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCEEEEECCCEEEE
AHGHSSTVPATMDMMVMHAQAVRRGAPRTFTLGCMPYQSYNTVDRALLNATRFMQDGGCD
ECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCC
AVKPQGGRSQAHILKALVDSGIPTASHIGLTPHTIAMFGGFKIQGRTAEAAMKILEDAFA
CCCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCHHHHHCCCEEEECCCHHHHHHHHHHHHH
IQDAGCFMLEFEAVPAKIATLISKQLEIPTIGIGAGAGCDGQILLSYDLLGVFTDFKPKF
HHCCCEEEEEECHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEHHHHHHHCCCHHH
TKRYANLTEVAVQGLKAYVDEVKTGRFPDDDHSYGVDDREYEQFMNLVEKRRHV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA