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The map label for this gene is dctA2 [H]

Identifier: 124265989

GI number: 124265989

Start: 830811

End: 832124

Strand: Direct

Name: dctA2 [H]

Synonym: Mpe_A0796

Alternate gene names: 124265989

Gene position: 830811-832124 (Clockwise)

Preceding gene: 124265976

Following gene: 124265993

Centisome position: 20.54

GC content: 67.66

Gene sequence:

>1314_bases
ATGCCCAAGTTCTTGCGTTCCCTGTTCGGCCAGGTGGTGCTGGCCCTCGTGCTGGGCGTGCTGCTGGGCCTGCTGTGGCC
CGAGACGGCGGTGAAGCTCAAGCCGCTGGGCGATGCCTTCATCAAGCTCATCAAGATGATCATTCCGGTGCTGGTGTTCT
GCGTCGTGGTGCATGGCATCGCCGGCGCGGGCGACCTGAAGCGCGTGGGACGGGTTGGCGTGAAGGCGCTGGTGTACTTC
GAGGTCGTCACCGCGGTCGCGCTGGCGCTCGGGCTCGCGCTAGGCTACCTGTTTCAGCCGGGGGTCGGCATGAACGTGGA
TCCCACCACGCTGGACGCCAAGGCCATGAGCGCCTATGCGGACAACGCCAGCAAGCTCACCGGCGGCGGCACCGTCGAGT
TCCTGCTGAAGCTGATCCCGACCACCGTGGTCGCCGCCTTCGCGACCGGCGACGTGCTGCAGGTGCTGCTGTTCGCGGTG
CTGTTCGGCTGCGCGCTGGCGCTCGTGGGCGAGAAGGGCCGTGCCGTGGCCGGGCTGATCGACGAACTGTCGTTGGTGCT
GTTCAAGATCATGGGGCTGATCATCAAGCTGGCGCCGCTGGGGGTGCTGGGCGCGATCGCCTTCACGGTCGGCAAGTACG
GTGTCGGCTCGCTGAAGCAACTGGGCCTGCTGGTCCTGCTGTTCTACGGGGCGGTGGTGCTGTTCGTCGCGGTGGTGCTG
GGCGGCATCATGCGAGCCTCGGGCTTCAGCCTGTGGAAGCTGCTGCGCTACCTGCGCGAGGAACTCGCGGTGGTGTTCGC
CACCACCTCGTCCGACAGCGTGCTGCCGCAGATCATGGCCAAGCTGCGCCACATGGGCATCCGCGATTCGACCGTGGGCC
TGGTGATCCCGACCGGCTACTCCTTCAACCTGGACGCGTTCTCGATCTACATCACGCTCGCCGCGGTGTTCATCGCCCAG
GCGACCAACACGCCGATCACGATGACCGACCTGCTGACCATCCTGGCGATCTCGCTGGTGACCAGCAAGGGCGCGCATGG
CGTGCCCGGTTCGGCCATCGTGGTGCTGGCCGCCACGCTGCAGGCCATCCCGGCGATTCCCGCGATCGGGCTGGTGCTGG
TGCTTTCCGTCGACTGGTTCATGGGCATCGCGCGTGCGCTCGGCAACCTGATCGGCAACTGCGTGGCGACGGTGGCGGTG
GCCGCCTGGGAGGGCGACATCGACCGCGAGCGGGCGCACAAGGTGCTGGACGGTGAGGCGGTGCCGGCGCACGACGTGGC
GGGCGCGCCGGGTGTGGCGGTAACGGCGTCCTGA

Upstream 100 bases:

>100_bases
CGGCGGGCCCGCCGGGGCGGCGCGCCGCGATGCGGGCACCGAGCTTGCATGACCCGCCGGGCGCTTCGCCCCTCCACCGT
TCGCTGCCCGGGAGCCTGCC

Downstream 100 bases:

>100_bases
GTTGATACGGAGCACAGGCGGTCCGGCCTCGGCGTGTGCGCCGCGCCGGTCCGCTGTGTTGCGTTCGTGTCCCCCGGCGT
CGGCCTGATCGGCCGCCGCC

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MPKFLRSLFGQVVLALVLGVLLGLLWPETAVKLKPLGDAFIKLIKMIIPVLVFCVVVHGIAGAGDLKRVGRVGVKALVYF
EVVTAVALALGLALGYLFQPGVGMNVDPTTLDAKAMSAYADNASKLTGGGTVEFLLKLIPTTVVAAFATGDVLQVLLFAV
LFGCALALVGEKGRAVAGLIDELSLVLFKIMGLIIKLAPLGVLGAIAFTVGKYGVGSLKQLGLLVLLFYGAVVLFVAVVL
GGIMRASGFSLWKLLRYLREELAVVFATTSSDSVLPQIMAKLRHMGIRDSTVGLVIPTGYSFNLDAFSIYITLAAVFIAQ
ATNTPITMTDLLTILAISLVTSKGAHGVPGSAIVVLAATLQAIPAIPAIGLVLVLSVDWFMGIARALGNLIGNCVATVAV
AAWEGDIDRERAHKVLDGEAVPAHDVAGAPGVAVTAS

Sequences:

>Translated_437_residues
MPKFLRSLFGQVVLALVLGVLLGLLWPETAVKLKPLGDAFIKLIKMIIPVLVFCVVVHGIAGAGDLKRVGRVGVKALVYF
EVVTAVALALGLALGYLFQPGVGMNVDPTTLDAKAMSAYADNASKLTGGGTVEFLLKLIPTTVVAAFATGDVLQVLLFAV
LFGCALALVGEKGRAVAGLIDELSLVLFKIMGLIIKLAPLGVLGAIAFTVGKYGVGSLKQLGLLVLLFYGAVVLFVAVVL
GGIMRASGFSLWKLLRYLREELAVVFATTSSDSVLPQIMAKLRHMGIRDSTVGLVIPTGYSFNLDAFSIYITLAAVFIAQ
ATNTPITMTDLLTILAISLVTSKGAHGVPGSAIVVLAATLQAIPAIPAIGLVLVLSVDWFMGIARALGNLIGNCVATVAV
AAWEGDIDRERAHKVLDGEAVPAHDVAGAPGVAVTAS
>Mature_436_residues
PKFLRSLFGQVVLALVLGVLLGLLWPETAVKLKPLGDAFIKLIKMIIPVLVFCVVVHGIAGAGDLKRVGRVGVKALVYFE
VVTAVALALGLALGYLFQPGVGMNVDPTTLDAKAMSAYADNASKLTGGGTVEFLLKLIPTTVVAAFATGDVLQVLLFAVL
FGCALALVGEKGRAVAGLIDELSLVLFKIMGLIIKLAPLGVLGAIAFTVGKYGVGSLKQLGLLVLLFYGAVVLFVAVVLG
GIMRASGFSLWKLLRYLREELAVVFATTSSDSVLPQIMAKLRHMGIRDSTVGLVIPTGYSFNLDAFSIYITLAAVFIAQA
TNTPITMTDLLTILAISLVTSKGAHGVPGSAIVVLAATLQAIPAIPAIGLVLVLSVDWFMGIARALGNLIGNCVATVAVA
AWEGDIDRERAHKVLDGEAVPAHDVAGAPGVAVTAS

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI4827012, Length=376, Percent_Identity=24.7340425531915, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI21314632, Length=404, Percent_Identity=28.4653465346535, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI169790839, Length=446, Percent_Identity=22.8699551569507, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI66773030, Length=455, Percent_Identity=24.3956043956044, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI194239697, Length=484, Percent_Identity=23.5537190082645, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI5032093, Length=418, Percent_Identity=26.3157894736842, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI223468566, Length=294, Percent_Identity=25.8503401360544, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI223468564, Length=294, Percent_Identity=25.8503401360544, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI40254478, Length=446, Percent_Identity=23.3183856502242, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI301601644, Length=165, Percent_Identity=30.9090909090909, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI262359914, Length=385, Percent_Identity=22.3376623376623, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1789947, Length=416, Percent_Identity=53.125, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1790514, Length=423, Percent_Identity=39.9527186761229, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1789473, Length=333, Percent_Identity=26.1261261261261, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788024, Length=398, Percent_Identity=23.3668341708543, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17537407, Length=424, Percent_Identity=24.5283018867925, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI193206654, Length=437, Percent_Identity=21.5102974828375, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71996953, Length=458, Percent_Identity=23.3624454148472, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193206505, Length=443, Percent_Identity=22.1218961625282, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71983099, Length=476, Percent_Identity=22.6890756302521, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71983106, Length=476, Percent_Identity=22.6890756302521, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17541374, Length=411, Percent_Identity=22.8710462287105, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583025, Length=427, Percent_Identity=23.8875878220141, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI17137668, Length=427, Percent_Identity=23.8875878220141, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24583023, Length=427, Percent_Identity=23.8875878220141, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI281360483, Length=482, Percent_Identity=25.7261410788382, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17137666, Length=482, Percent_Identity=25.7261410788382, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281360481, Length=482, Percent_Identity=25.7261410788382, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45364; Mature: 45233

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCEEECC
>Mature Secondary Structure 
PKFLRSLFGQVVLALVLGVLLGLLWPETAVKLKPLGDAFIKLIKMIIPVLVFCVVVHGI
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
AGAGDLKRVGRVGVKALVYFEVVTAVALALGLALGYLFQPGVGMNVDPTTLDAKAMSAYA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
DNASKLTGGGTVEFLLKLIPTTVVAAFATGDVLQVLLFAVLFGCALALVGEKGRAVAGLI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
DELSLVLFKIMGLIIKLAPLGVLGAIAFTVGKYGVGSLKQLGLLVLLFYGAVVLFVAVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GGIMRASGFSLWKLLRYLREELAVVFATTSSDSVLPQIMAKLRHMGIRDSTVGLVIPTGY
HHHHHHCCHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEECCC
SFNLDAFSIYITLAAVFIAQATNTPITMTDLLTILAISLVTSKGAHGVPGSAIVVLAATL
CCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
QAIPAIPAIGLVLVLSVDWFMGIARALGNLIGNCVATVAVAAWEGDIDRERAHKVLDGEA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
VPAHDVAGAPGVAVTAS
CCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]