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The map label for this gene is pilB [H]

Identifier: 124265702

GI number: 124265702

Start: 547297

End: 549015

Strand: Reverse

Name: pilB [H]

Synonym: Mpe_A0509

Alternate gene names: 124265702

Gene position: 549015-547297 (Counterclockwise)

Preceding gene: 124265703

Following gene: 124265701

Centisome position: 13.58

GC content: 63.06

Gene sequence:

>1719_bases
GTGGATACCCTCGCCGATGCCCCGCAGAGTTCGCTGTCCGGCGTCGCGCGCGTGCTCGTGCACGCGGGCAAGCTCAACGT
CAAGACCGCCGAAGATCTGGTCCGCAGCGCCAAGGAGCAGAAGCGCAGTTTCATCTCGGCGGTCCTGAGCGCTGGCGCCG
TCAACCCTTCGGATCTTGCCCATACGCTGTCCAGCGTGCTGGCGATGCCCTTGCTCGATCTGGCGGCGGTCGATCCGCAG
CGCCTGCCTCGCAACGTCATCGATGCCAAGCTGGCCACCCAGTATCAGGTATTGGTGCTGGGCAAGCGCGGCAACCGCCT
CTTCATCGCCGGGGCCGACCCCACCGACCAGGAGGCCGCTGAACGCATCAAGTTCGCAACGCAGCTGACGCCCGAGTGGG
TGATCGTCGAGCACGACAAGCTGTCCCGGCTCGTCGATGCAGCTACGACAACGGCCAGCGAGTCTCTCGAGTCGCTGGTC
GCCGGTGATTTCGAGTTCGACGTCACCGAGGAAGACACCAGCGCATCCGACGCCGCCGAGGTCACCACCGACGTCGAGGA
CGCACCGGTCGTTCGCTTCCTTCAGAAGATGCTGATCGATGCGATCAACGCACGAGCCTCCGATCTGCACTTCGAGCCGT
TCGAATACAACTACCGGGTCCGCTTCCGCGTCGATGGCGAACTGCGCGAGATCACGCAACCGCCGATTGCCATCAAGGAC
AAGCTCGCCTCCCGCATCAAGGTGATTTCCCGGCTCGACATTGCCGAGAAGCGCGTGCCGCAGGACGGCCGCATGAAGCT
CAAGTTCGGCAGCAAGGCGATTGATTTCCGGGTCAGCACCCTGCCCACGCTGTTCGCCGAGAAGATCGTGATCCGTATCC
TCGACCCGTCGAGTGCCAAGCTCGGCATCGAGGCGCTCGGCTACGAGAAGGTCGAGAAAGACCGCTTGCTGGAAGCCATC
AAGCGCCCCTATGGCATGGTGCTGGTCACCGGTCCCACAGGCTCCGGCAAGACGGTGTCTCTGTACACCTGCCTGAACAT
CCTGAACCAACCCGGCGTCAATATCTCCACGGTGGAGGACCCGGCCGAAATCAACCTCCCGGGCGTCAACCAGGTCAACG
TCAACGACAAGGCAGGACTGAACTTCTCGGTCGCCTTGAAGGCCTTCCTGCGGCAGGATCCGGACGTCATCATGGTCGGC
GAAATTCGCGATCTCGACACGGCGGACATCGCCATCAAGGCGGCGCAGACCGGGCACATGGTGATGAGCACGCTGCATAC
CAACGACGCGCCGACGACGCTGACCCGCCTGATGAACATGGGCGTCGCGCCGTTCAACATCGCCTCCAGCGTGATCCTGA
TCACGGCGCAGCGGCTGGCGCGCAAACTCTGCGAGAACTGCAAGGCGCCGGCCGATTACCCTCGCGAGGCGTTGCTTCGC
GCCGGCTTCGCTGAATCGGACCTGGATGGCAGTTGGAAGCCCTATCGTGCCGTCGGCTGCTCCAGTTGCAGCAATGGCTA
CCGGGGGCGTGTCGGCATCTACCAGGTCATGCCGATCAGCGAAGAGATCCAGCGCATCATCCTGACGCAGGGCAATGCGG
TCGACATCGCAAAGCAGGCACAGCAAGAGGGCGTGCGCGATTTGCGTCAGTCGGGTCTGGTCAAGGTGCGAGCGGGTGTG
ACCACCCTGGAAGAAGTCATCTCGGTCACCAACGAGTAA

Upstream 100 bases:

>100_bases
CGCGTGGTGGATGCTCACATCCGCGGACCTCGGTCGTTTACAGGGCCGCATGAATCGGCGATCATGATTCGGTTGCAGCC
CTGACTTCGCCGATTGCCCT

Downstream 100 bases:

>100_bases
GCCCATTAGGCGGCGGGGAGAGTCGGAGACACCATGGCCACAGCAGCAACGGCTTCGGCGCGAGACATCAAGGAATTCGT
CTTCGAGTGGGAAGGCAAGG

Product: pilus biogenesis ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MDTLADAPQSSLSGVARVLVHAGKLNVKTAEDLVRSAKEQKRSFISAVLSAGAVNPSDLAHTLSSVLAMPLLDLAAVDPQ
RLPRNVIDAKLATQYQVLVLGKRGNRLFIAGADPTDQEAAERIKFATQLTPEWVIVEHDKLSRLVDAATTTASESLESLV
AGDFEFDVTEEDTSASDAAEVTTDVEDAPVVRFLQKMLIDAINARASDLHFEPFEYNYRVRFRVDGELREITQPPIAIKD
KLASRIKVISRLDIAEKRVPQDGRMKLKFGSKAIDFRVSTLPTLFAEKIVIRILDPSSAKLGIEALGYEKVEKDRLLEAI
KRPYGMVLVTGPTGSGKTVSLYTCLNILNQPGVNISTVEDPAEINLPGVNQVNVNDKAGLNFSVALKAFLRQDPDVIMVG
EIRDLDTADIAIKAAQTGHMVMSTLHTNDAPTTLTRLMNMGVAPFNIASSVILITAQRLARKLCENCKAPADYPREALLR
AGFAESDLDGSWKPYRAVGCSSCSNGYRGRVGIYQVMPISEEIQRIILTQGNAVDIAKQAQQEGVRDLRQSGLVKVRAGV
TTLEEVISVTNE

Sequences:

>Translated_572_residues
MDTLADAPQSSLSGVARVLVHAGKLNVKTAEDLVRSAKEQKRSFISAVLSAGAVNPSDLAHTLSSVLAMPLLDLAAVDPQ
RLPRNVIDAKLATQYQVLVLGKRGNRLFIAGADPTDQEAAERIKFATQLTPEWVIVEHDKLSRLVDAATTTASESLESLV
AGDFEFDVTEEDTSASDAAEVTTDVEDAPVVRFLQKMLIDAINARASDLHFEPFEYNYRVRFRVDGELREITQPPIAIKD
KLASRIKVISRLDIAEKRVPQDGRMKLKFGSKAIDFRVSTLPTLFAEKIVIRILDPSSAKLGIEALGYEKVEKDRLLEAI
KRPYGMVLVTGPTGSGKTVSLYTCLNILNQPGVNISTVEDPAEINLPGVNQVNVNDKAGLNFSVALKAFLRQDPDVIMVG
EIRDLDTADIAIKAAQTGHMVMSTLHTNDAPTTLTRLMNMGVAPFNIASSVILITAQRLARKLCENCKAPADYPREALLR
AGFAESDLDGSWKPYRAVGCSSCSNGYRGRVGIYQVMPISEEIQRIILTQGNAVDIAKQAQQEGVRDLRQSGLVKVRAGV
TTLEEVISVTNE
>Mature_572_residues
MDTLADAPQSSLSGVARVLVHAGKLNVKTAEDLVRSAKEQKRSFISAVLSAGAVNPSDLAHTLSSVLAMPLLDLAAVDPQ
RLPRNVIDAKLATQYQVLVLGKRGNRLFIAGADPTDQEAAERIKFATQLTPEWVIVEHDKLSRLVDAATTTASESLESLV
AGDFEFDVTEEDTSASDAAEVTTDVEDAPVVRFLQKMLIDAINARASDLHFEPFEYNYRVRFRVDGELREITQPPIAIKD
KLASRIKVISRLDIAEKRVPQDGRMKLKFGSKAIDFRVSTLPTLFAEKIVIRILDPSSAKLGIEALGYEKVEKDRLLEAI
KRPYGMVLVTGPTGSGKTVSLYTCLNILNQPGVNISTVEDPAEINLPGVNQVNVNDKAGLNFSVALKAFLRQDPDVIMVG
EIRDLDTADIAIKAAQTGHMVMSTLHTNDAPTTLTRLMNMGVAPFNIASSVILITAQRLARKLCENCKAPADYPREALLR
AGFAESDLDGSWKPYRAVGCSSCSNGYRGRVGIYQVMPISEEIQRIILTQGNAVDIAKQAQQEGVRDLRQSGLVKVRAGV
TTLEEVISVTNE

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=387, Percent_Identity=44.9612403100775, Blast_Score=332, Evalue=6e-92,
Organism=Escherichia coli, GI1786296, Length=389, Percent_Identity=42.6735218508997, Blast_Score=329, Evalue=4e-91,
Organism=Escherichia coli, GI87082188, Length=163, Percent_Identity=38.6503067484663, Blast_Score=99, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62302; Mature: 62302

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLADAPQSSLSGVARVLVHAGKLNVKTAEDLVRSAKEQKRSFISAVLSAGAVNPSDLA
CCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
HTLSSVLAMPLLDLAAVDPQRLPRNVIDAKLATQYQVLVLGKRGNRLFIAGADPTDQEAA
HHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHCEEEEEEEECCCCEEEEECCCCCHHHHH
ERIKFATQLTPEWVIVEHDKLSRLVDAATTTASESLESLVAGDFEFDVTEEDTSASDAAE
HHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHH
VTTDVEDAPVVRFLQKMLIDAINARASDLHFEPFEYNYRVRFRVDGELREITQPPIAIKD
HHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCHHHHHCCCCHHHHH
KLASRIKVISRLDIAEKRVPQDGRMKLKFGSKAIDFRVSTLPTLFAEKIVIRILDPSSAK
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHEEEEECCCCCC
LGIEALGYEKVEKDRLLEAIKRPYGMVLVTGPTGSGKTVSLYTCLNILNQPGVNISTVED
CCHHHHCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEHHHHHHHHCCCCCEEEECCC
PAEINLPGVNQVNVNDKAGLNFSVALKAFLRQDPDVIMVGEIRDLDTADIAIKAAQTGHM
CCCCCCCCCCEECCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCHH
VMSTLHTNDAPTTLTRLMNMGVAPFNIASSVILITAQRLARKLCENCKAPADYPREALLR
HHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
AGFAESDLDGSWKPYRAVGCSSCSNGYRGRVGIYQVMPISEEIQRIILTQGNAVDIAKQA
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCHHHHHHH
QQEGVRDLRQSGLVKVRAGVTTLEEVISVTNE
HHHHHHHHHHCCCEEEECCHHHHHHHHHHCCC
>Mature Secondary Structure
MDTLADAPQSSLSGVARVLVHAGKLNVKTAEDLVRSAKEQKRSFISAVLSAGAVNPSDLA
CCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
HTLSSVLAMPLLDLAAVDPQRLPRNVIDAKLATQYQVLVLGKRGNRLFIAGADPTDQEAA
HHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHCEEEEEEEECCCCEEEEECCCCCHHHHH
ERIKFATQLTPEWVIVEHDKLSRLVDAATTTASESLESLVAGDFEFDVTEEDTSASDAAE
HHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHH
VTTDVEDAPVVRFLQKMLIDAINARASDLHFEPFEYNYRVRFRVDGELREITQPPIAIKD
HHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCHHHHHCCCCHHHHH
KLASRIKVISRLDIAEKRVPQDGRMKLKFGSKAIDFRVSTLPTLFAEKIVIRILDPSSAK
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHEEEEECCCCCC
LGIEALGYEKVEKDRLLEAIKRPYGMVLVTGPTGSGKTVSLYTCLNILNQPGVNISTVED
CCHHHHCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEHHHHHHHHCCCCCEEEECCC
PAEINLPGVNQVNVNDKAGLNFSVALKAFLRQDPDVIMVGEIRDLDTADIAIKAAQTGHM
CCCCCCCCCCEECCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCHH
VMSTLHTNDAPTTLTRLMNMGVAPFNIASSVILITAQRLARKLCENCKAPADYPREALLR
HHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
AGFAESDLDGSWKPYRAVGCSSCSNGYRGRVGIYQVMPISEEIQRIILTQGNAVDIAKQA
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCHHHHHHH
QQEGVRDLRQSGLVKVRAGVTTLEEVISVTNE
HHHHHHHHHHCCCEEEECCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]