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The map label for this gene is ispB [H]

Identifier: 124265703

GI number: 124265703

Start: 549277

End: 550269

Strand: Reverse

Name: ispB [H]

Synonym: Mpe_A0510

Alternate gene names: 124265703

Gene position: 550269-549277 (Counterclockwise)

Preceding gene: 124265709

Following gene: 124265702

Centisome position: 13.61

GC content: 64.45

Gene sequence:

>993_bases
GTGACTGCCGTCTCGAACGTCTCCGGGTCGACCCTTTCCTTCGCAGACCTGGTCGCCGCCGACATGGGCGAGGTCGATGC
GTTGATCCGGCGCCGACTGGCTTCGGATGTCGCGCTGATCAACCAGATATCGCACTACATCATCAGCGCCGGTGGCAAGC
GGTTACGTCCAATGCTGGTGCTGTTGTTCGCGCAGGCACTCGGCTTCCGGGGTCCAGAGCGCTTCGAACTGGCGGCGACG
GTCGAGTTCATCCACACCGCGACCTTGCTGCACGACGACGTCGTCGACGAGTCCGCGCTGCGGCGCGGACGTGACACGGC
CAATGCGCGTTTCGGCAATGCAGCGAGCGTGCTGGTTGGCGACTTCCTTTATTCGCGCGCCTTCCAGATGATGGTCTCCG
TGAACCGCATGAGGGTTCTGGAAGTGCTGGCCGACGCCACGAATGTGATCGCCGAGGGCGAAGTCCTGCAGTTGATGAAC
ATGCACGATCCCGACCTGTCCATCGACGACTATCTCCGGGTGATCCGGTTCAAAACGGCCAAGCTGTTCGAGGCCAGCGC
TCGGCTCGGCGCCATGCTGGCCGACGCCTCCCCCGGCGTCGAAACCGCGTGCGCCGATTACGGGAGGGCGCTGGGCACCG
CATTCCAGTTGGTCGACGACCTGCTGGACTATGAAGGCGCCACGTCAGCACTGGGAAAGAACATCGGCGACGACCTGCGG
GAAGGCAAGCCGACGCTGCCCTTGCTGGTGGCCATGACGCGAGGGACGCCGGCGCAAAGGGACGTCGTGCGTGCGGCCAT
CGAGCAAGGCGAGGTGCAGCGCCTGCCGGAGATCATCGAGATCGTCCGATCCACCGGCGCCTTGGAAGCCACCAGGGAAG
TGGCGCGCGCCGAGGTCGCCCGAGCCTGCGAAGCCCTGTCGGTCTTGCCAGACTCGCAGTACCGCGAAGCTCTGCTACAC
TTATGTGCTCAGTCGACAGAGCGTTCCTCGTGA

Upstream 100 bases:

>100_bases
ATGCACCGGGTACGCTGGGCGCCCTGAGCCGCAGGTCGCGCGCGATCCCCCTCCCTATAATCGAAACGCTCGAAATTCCT
CGAGTTCCTCCTCCTTCAGC

Downstream 100 bases:

>100_bases
ACCGTTGACCTGAGCAGTCAGCATCGATCTGACCCAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGT
AGAGGCCGGAGGTTCGAATC

Product: dimethylallyltransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTAVSNVSGSTLSFADLVAADMGEVDALIRRRLASDVALINQISHYIISAGGKRLRPMLVLLFAQALGFRGPERFELAAT
VEFIHTATLLHDDVVDESALRRGRDTANARFGNAASVLVGDFLYSRAFQMMVSVNRMRVLEVLADATNVIAEGEVLQLMN
MHDPDLSIDDYLRVIRFKTAKLFEASARLGAMLADASPGVETACADYGRALGTAFQLVDDLLDYEGATSALGKNIGDDLR
EGKPTLPLLVAMTRGTPAQRDVVRAAIEQGEVQRLPEIIEIVRSTGALEATREVARAEVARACEALSVLPDSQYREALLH
LCAQSTERSS

Sequences:

>Translated_330_residues
MTAVSNVSGSTLSFADLVAADMGEVDALIRRRLASDVALINQISHYIISAGGKRLRPMLVLLFAQALGFRGPERFELAAT
VEFIHTATLLHDDVVDESALRRGRDTANARFGNAASVLVGDFLYSRAFQMMVSVNRMRVLEVLADATNVIAEGEVLQLMN
MHDPDLSIDDYLRVIRFKTAKLFEASARLGAMLADASPGVETACADYGRALGTAFQLVDDLLDYEGATSALGKNIGDDLR
EGKPTLPLLVAMTRGTPAQRDVVRAAIEQGEVQRLPEIIEIVRSTGALEATREVARAEVARACEALSVLPDSQYREALLH
LCAQSTERSS
>Mature_329_residues
TAVSNVSGSTLSFADLVAADMGEVDALIRRRLASDVALINQISHYIISAGGKRLRPMLVLLFAQALGFRGPERFELAATV
EFIHTATLLHDDVVDESALRRGRDTANARFGNAASVLVGDFLYSRAFQMMVSVNRMRVLEVLADATNVIAEGEVLQLMNM
HDPDLSIDDYLRVIRFKTAKLFEASARLGAMLADASPGVETACADYGRALGTAFQLVDDLLDYEGATSALGKNIGDDLRE
GKPTLPLLVAMTRGTPAQRDVVRAAIEQGEVQRLPEIIEIVRSTGALEATREVARAEVARACEALSVLPDSQYREALLHL
CAQSTERSS

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=300, Percent_Identity=30, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI4758430, Length=268, Percent_Identity=22.7611940298507, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI83700220, Length=268, Percent_Identity=22.7611940298507, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1789578, Length=314, Percent_Identity=57.9617834394904, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1786623, Length=253, Percent_Identity=33.9920948616601, Blast_Score=104, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17505681, Length=320, Percent_Identity=32.5, Blast_Score=154, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6319475, Length=295, Percent_Identity=35.2542372881356, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6325188, Length=259, Percent_Identity=24.3243243243243, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24651612, Length=287, Percent_Identity=31.3588850174216, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI281365769, Length=271, Percent_Identity=25.830258302583, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24660002, Length=271, Percent_Identity=25.830258302583, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35735; Mature: 35604

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVSNVSGSTLSFADLVAADMGEVDALIRRRLASDVALINQISHYIISAGGKRLRPMLV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LLFAQALGFRGPERFELAATVEFIHTATLLHDDVVDESALRRGRDTANARFGNAASVLVG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
DFLYSRAFQMMVSVNRMRVLEVLADATNVIAEGEVLQLMNMHDPDLSIDDYLRVIRFKTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHH
KLFEASARLGAMLADASPGVETACADYGRALGTAFQLVDDLLDYEGATSALGKNIGDDLR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EGKPTLPLLVAMTRGTPAQRDVVRAAIEQGEVQRLPEIIEIVRSTGALEATREVARAEVA
CCCCCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RACEALSVLPDSQYREALLHLCAQSTERSS
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TAVSNVSGSTLSFADLVAADMGEVDALIRRRLASDVALINQISHYIISAGGKRLRPMLV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LLFAQALGFRGPERFELAATVEFIHTATLLHDDVVDESALRRGRDTANARFGNAASVLVG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
DFLYSRAFQMMVSVNRMRVLEVLADATNVIAEGEVLQLMNMHDPDLSIDDYLRVIRFKTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHH
KLFEASARLGAMLADASPGVETACADYGRALGTAFQLVDDLLDYEGATSALGKNIGDDLR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EGKPTLPLLVAMTRGTPAQRDVVRAAIEQGEVQRLPEIIEIVRSTGALEATREVARAEVA
CCCCCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RACEALSVLPDSQYREALLHLCAQSTERSS
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]