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The map label for this gene is pgk

Identifier: 124024485

GI number: 124024485

Start: 2462969

End: 2464174

Strand: Reverse

Name: pgk

Synonym: P9303_27971

Alternate gene names: 124024485

Gene position: 2464174-2462969 (Counterclockwise)

Preceding gene: 124024491

Following gene: 124024483

Centisome position: 91.86

GC content: 57.38

Gene sequence:

>1206_bases
ATGGCGAAGCGTTCACTGTCCAGCCTCAGCGCAGCGGACCTACGTAGTAAGCGTGTGCTGGTTCGGGTTGACTTCAACGT
GCCTCTCAACGAGAACGGCGCCATTACCGATGACACCCGCATCCGCGCCGCCCTGCCCACCATCAATGACCTGATCGACA
AAGGTGCTCGGGTGATCCTCTCGGCCCACTTTGGCCGACCAAAGGGTCAAATCAATGAAGGAATGCGGCTCACACCAGTA
GCAAAACGCCTCAGCGAACTGCTGGGTAAAACTGTGATAAAAACCGAAAGCTGTGTCGGTAGCGACGCCGAAGCCAAGGT
CAATGCCATGGCCGATGGAGACGTCGTACTGCTCGAGAATGTGCGCTTTTTCTCAGAAGAAGAAAAAAACGACGCCAACT
TCGCTCAAAAGCTCGCAGCACTTGCTGACGCCTATGTGAATGATGCTTTCGGTGCCGCACACCGTGCCCACGCCTCTACA
GAAGGGGTCACCAAATTCCTCAACCCCAGTGTGGCAGGCCACCTGATGGAGAAGGAACTTCAATACCTGCAAGGTGCCAT
CGACGACCCCAAGCGACCCCTTGCAGCGATCGTGGGTGGGTCCAAGGTCAGCAGCAAGATCGGCGTGTTGGAAGCCTTGA
TCGACAAGTGCGACAAGGTGCTGATCGGCGGCGGCATGATCTTCACGTTCTACAAAGCGCGTGGCCTTTCAGTAGGCAAG
AGCCTGGTGGAAGAGGACAAGCTGGAACTCGCCAAGGAGCTAGAGGCCAAGGCCAAGGCCAAGGGGGTTCAACTGCTTCT
GCCCACTGATGTTGTTCTAGCCGATGACTTCTCCCCTGATGCCAATAGTCAGACCGTATCCATCGACGCCATTCCCGATG
GTTGGATGGGCCTGGACATTGGCCCAGACTCAATCAATCTCTTCCAAGACGCACTGGCTGACTGCCAAACAGTGATCTGG
AACGGCCCCATGGGCGTGTTTGAGTTCGACAAGTTTGCCGCCGGCACCAACGCCATCGCCACCACCTTGGCGGACCTCAG
TGGCAAGGGTTGCTGCACAATCATCGGCGGTGGTGACTCCGTTGCCGCTGTCGAGAAGGCCGGCCTCGCCGAGAAGATGT
CCCACATCTCTACTGGTGGTGGCGCCAGCCTTGAGCTGCTGGAAGGCAAGGTGTTGCCAGGTGTCGCCGCTCTCGACGAC
GCTTAA

Upstream 100 bases:

>100_bases
TAGTGCCAACCAGATCAGTTCGCCGGCCCATCAACGCAACCAGGCAAGCAAAACACGGGTTAATGTCAGCGGGTTTTTCT
TACCGCACGGCACAACCCCC

Downstream 100 bases:

>100_bases
GCAAAGGCCTCGCTTGTGTTCTCGGGCCATTGCCTTGCATAACGGCAATGGCCCCCGCATTCATTAGCTCTGATAAGAGC
TCTCGAGCAAACTTCTGGCG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MAKRSLSSLSAADLRSKRVLVRVDFNVPLNENGAITDDTRIRAALPTINDLIDKGARVILSAHFGRPKGQINEGMRLTPV
AKRLSELLGKTVIKTESCVGSDAEAKVNAMADGDVVLLENVRFFSEEEKNDANFAQKLAALADAYVNDAFGAAHRAHAST
EGVTKFLNPSVAGHLMEKELQYLQGAIDDPKRPLAAIVGGSKVSSKIGVLEALIDKCDKVLIGGGMIFTFYKARGLSVGK
SLVEEDKLELAKELEAKAKAKGVQLLLPTDVVLADDFSPDANSQTVSIDAIPDGWMGLDIGPDSINLFQDALADCQTVIW
NGPMGVFEFDKFAAGTNAIATTLADLSGKGCCTIIGGGDSVAAVEKAGLAEKMSHISTGGGASLELLEGKVLPGVAALDD
A

Sequences:

>Translated_401_residues
MAKRSLSSLSAADLRSKRVLVRVDFNVPLNENGAITDDTRIRAALPTINDLIDKGARVILSAHFGRPKGQINEGMRLTPV
AKRLSELLGKTVIKTESCVGSDAEAKVNAMADGDVVLLENVRFFSEEEKNDANFAQKLAALADAYVNDAFGAAHRAHAST
EGVTKFLNPSVAGHLMEKELQYLQGAIDDPKRPLAAIVGGSKVSSKIGVLEALIDKCDKVLIGGGMIFTFYKARGLSVGK
SLVEEDKLELAKELEAKAKAKGVQLLLPTDVVLADDFSPDANSQTVSIDAIPDGWMGLDIGPDSINLFQDALADCQTVIW
NGPMGVFEFDKFAAGTNAIATTLADLSGKGCCTIIGGGDSVAAVEKAGLAEKMSHISTGGGASLELLEGKVLPGVAALDD
A
>Mature_400_residues
AKRSLSSLSAADLRSKRVLVRVDFNVPLNENGAITDDTRIRAALPTINDLIDKGARVILSAHFGRPKGQINEGMRLTPVA
KRLSELLGKTVIKTESCVGSDAEAKVNAMADGDVVLLENVRFFSEEEKNDANFAQKLAALADAYVNDAFGAAHRAHASTE
GVTKFLNPSVAGHLMEKELQYLQGAIDDPKRPLAAIVGGSKVSSKIGVLEALIDKCDKVLIGGGMIFTFYKARGLSVGKS
LVEEDKLELAKELEAKAKAKGVQLLLPTDVVLADDFSPDANSQTVSIDAIPDGWMGLDIGPDSINLFQDALADCQTVIWN
GPMGVFEFDKFAAGTNAIATTLADLSGKGCCTIIGGGDSVAAVEKAGLAEKMSHISTGGGASLELLEGKVLPGVAALDDA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=412, Percent_Identity=45.3883495145631, Blast_Score=345, Evalue=3e-95,
Organism=Homo sapiens, GI31543397, Length=412, Percent_Identity=43.6893203883495, Blast_Score=339, Evalue=2e-93,
Organism=Escherichia coli, GI1789294, Length=398, Percent_Identity=43.21608040201, Blast_Score=299, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17508823, Length=407, Percent_Identity=48.6486486486487, Blast_Score=369, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6319857, Length=417, Percent_Identity=48.9208633093525, Blast_Score=375, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17136394, Length=412, Percent_Identity=47.0873786407767, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI24581203, Length=413, Percent_Identity=43.5835351089588, Blast_Score=320, Evalue=8e-88,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_PROM3 (A2CDG7)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001018792.1
- ProteinModelPortal:   A2CDG7
- SMR:   A2CDG7
- STRING:   A2CDG7
- GeneID:   4778764
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_27971
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42136; Mature: 42004

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 40-40 BINDING 122-122 BINDING 155-155 BINDING 206-206 BINDING 297-297 BINDING 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRSLSSLSAADLRSKRVLVRVDFNVPLNENGAITDDTRIRAALPTINDLIDKGARVIL
CCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCEECCCHHHHHHHCCCEEEE
SAHFGRPKGQINEGMRLTPVAKRLSELLGKTVIKTESCVGSDAEAKVNAMADGDVVLLEN
EECCCCCCCHHCCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEC
VRFFSEEEKNDANFAQKLAALADAYVNDAFGAAHRAHASTEGVTKFLNPSVAGHLMEKEL
CHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHH
QYLQGAIDDPKRPLAAIVGGSKVSSKIGVLEALIDKCDKVLIGGGMIFTFYKARGLSVGK
HHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCEEECCCEEEEHHHHCCCHHHH
SLVEEDKLELAKELEAKAKAKGVQLLLPTDVVLADDFSPDANSQTVSIDAIPDGWMGLDI
HHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCCCCCCCEEEEECCCCCCEEEEE
GPDSINLFQDALADCQTVIWNGPMGVFEFDKFAAGTNAIATTLADLSGKGCCTIIGGGDS
CCCHHHHHHHHHHHHHHEEECCCCCCEEHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCC
VAAVEKAGLAEKMSHISTGGGASLELLEGKVLPGVAALDDA
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHCCCC
>Mature Secondary Structure 
AKRSLSSLSAADLRSKRVLVRVDFNVPLNENGAITDDTRIRAALPTINDLIDKGARVIL
CCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCEECCCHHHHHHHCCCEEEE
SAHFGRPKGQINEGMRLTPVAKRLSELLGKTVIKTESCVGSDAEAKVNAMADGDVVLLEN
EECCCCCCCHHCCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEC
VRFFSEEEKNDANFAQKLAALADAYVNDAFGAAHRAHASTEGVTKFLNPSVAGHLMEKEL
CHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHH
QYLQGAIDDPKRPLAAIVGGSKVSSKIGVLEALIDKCDKVLIGGGMIFTFYKARGLSVGK
HHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCEEECCCEEEEHHHHCCCHHHH
SLVEEDKLELAKELEAKAKAKGVQLLLPTDVVLADDFSPDANSQTVSIDAIPDGWMGLDI
HHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCCCCCCCEEEEECCCCCCEEEEE
GPDSINLFQDALADCQTVIWNGPMGVFEFDKFAAGTNAIATTLADLSGKGCCTIIGGGDS
CCCHHHHHHHHHHHHHHEEECCCCCCEEHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCC
VAAVEKAGLAEKMSHISTGGGASLELLEGKVLPGVAALDDA
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA