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The map label for this gene is mmsB [H]

Identifier: 124024483

GI number: 124024483

Start: 2461719

End: 2462624

Strand: Reverse

Name: mmsB [H]

Synonym: P9303_27951

Alternate gene names: 124024483

Gene position: 2462624-2461719 (Counterclockwise)

Preceding gene: 124024485

Following gene: 124024482

Centisome position: 91.8

GC content: 55.52

Gene sequence:

>906_bases
ATGAGCAAAGCTCAGCTAAGCCCAAGGTCTAGCCTTGCCTTTGTTGGCCTCGGCGCATTGGGTTTACCAATGGCTGCCAA
TCTGCTGGCTGCGGGCTATGAGCTCAAGGTTCACAGCCGCAGTCGTGCGGCTGAACTGAATTTGGCTTTAAAAGGTAGCC
GACCCTGCAGCTCACCAGCAGAAGCTGCAGCAGATAGCCAGGCTCTATTGATTTGCGTTAGCGATGATGAAGCAGTAGAA
GCGGTTCTTTTTGGTTCTCAGGGAGCAGCTTCTAAGCTCAGCTCAGGCGCCGTTGTGGTGGATTTCTCGACGATCGCCCC
AGCAACGTCGATCACCCTTGCTGAGCGACTTGCTCAGCAAGGGGTGACCTATCTGGATGCACCGGTAACCGGAGGCACTG
AAGGGGCCAGAGCAGGAACTCTTACCGTTCTAGTGGGTGGCAACACACAAGCTCTGGCAAGGGTGCAGCCACTGCTGGAG
GTGATCGGTGAGAGCATTCATCACTTCGGTTCGGTGGGACGGGGCCAGCAGGTGAAGGCGCTCAACCAAGTGCTGGTGGC
AGGTAGCTATGCCGCATTAGCAGAAGCAATCGCGCTAGGGCAGCAGCTGGGTCTCCCTATGCCGGAGGTGATCACAGCCT
TACAGCATGGTGCCGCAGGCTCATGGGCCCTTCAACACCGATCAACAGCGATGCTGGAAGATCACTATCCGCTTGGCTTC
AAGCTGGCTTTGCATCACAAGGATCTCGGCATTGCCCTGGAAACGGCAGAACGCGTGGGACTACAACTGCCAATCACAAG
CAAAGTGAAGTCCATGGAGGCAAACCTGATTGAACTCGGCCATAGCGAGGAAGATGTATCAGTGCTGAGGCGGTGGTTTG
ATCAGCAACAGGCAGAATTTCAGTAA

Upstream 100 bases:

>100_bases
TCGTCGAGCATCACGAGGCAAGATCCTTAGTTTGCAGGTTTGAAGCAACTGTCAACGTTGAGCTGAAGCAGTAAAAATCG
AAGCAGCTCTACTGACAAAC

Downstream 100 bases:

>100_bases
TTCTGATCGAGCCTTCGATAGGCTGAATCAGTTGTTGATACCACTGCAATTGAAAGCTAGTACTTCATTCAAATAAAAGC
CAATGCATTCAGCTCGAATG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MSKAQLSPRSSLAFVGLGALGLPMAANLLAAGYELKVHSRSRAAELNLALKGSRPCSSPAEAAADSQALLICVSDDEAVE
AVLFGSQGAASKLSSGAVVVDFSTIAPATSITLAERLAQQGVTYLDAPVTGGTEGARAGTLTVLVGGNTQALARVQPLLE
VIGESIHHFGSVGRGQQVKALNQVLVAGSYAALAEAIALGQQLGLPMPEVITALQHGAAGSWALQHRSTAMLEDHYPLGF
KLALHHKDLGIALETAERVGLQLPITSKVKSMEANLIELGHSEEDVSVLRRWFDQQQAEFQ

Sequences:

>Translated_301_residues
MSKAQLSPRSSLAFVGLGALGLPMAANLLAAGYELKVHSRSRAAELNLALKGSRPCSSPAEAAADSQALLICVSDDEAVE
AVLFGSQGAASKLSSGAVVVDFSTIAPATSITLAERLAQQGVTYLDAPVTGGTEGARAGTLTVLVGGNTQALARVQPLLE
VIGESIHHFGSVGRGQQVKALNQVLVAGSYAALAEAIALGQQLGLPMPEVITALQHGAAGSWALQHRSTAMLEDHYPLGF
KLALHHKDLGIALETAERVGLQLPITSKVKSMEANLIELGHSEEDVSVLRRWFDQQQAEFQ
>Mature_300_residues
SKAQLSPRSSLAFVGLGALGLPMAANLLAAGYELKVHSRSRAAELNLALKGSRPCSSPAEAAADSQALLICVSDDEAVEA
VLFGSQGAASKLSSGAVVVDFSTIAPATSITLAERLAQQGVTYLDAPVTGGTEGARAGTLTVLVGGNTQALARVQPLLEV
IGESIHHFGSVGRGQQVKALNQVLVAGSYAALAEAIALGQQLGLPMPEVITALQHGAAGSWALQHRSTAMLEDHYPLGFK
LALHHKDLGIALETAERVGLQLPITSKVKSMEANLIELGHSEEDVSVLRRWFDQQQAEFQ

Specific function: Catalyzes the conversion of 2-formylglutarate to (S)-2- hydroxymethylglutarate. Has very low activity with (S)-3- hydroxyisobutyrate [H]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=298, Percent_Identity=30.8724832214765, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI40556376, Length=283, Percent_Identity=25.0883392226148, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI1786719, Length=278, Percent_Identity=37.410071942446, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI145693186, Length=284, Percent_Identity=34.5070422535211, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1790315, Length=286, Percent_Identity=31.8181818181818, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1789092, Length=290, Percent_Identity=31.3793103448276, Blast_Score=105, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17557316, Length=262, Percent_Identity=29.3893129770992, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24655230, Length=294, Percent_Identity=29.5918367346939, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI19922568, Length=294, Percent_Identity=29.5918367346939, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI28574115, Length=280, Percent_Identity=25, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.291 [H]

Molecular weight: Translated: 31370; Mature: 31239

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAQLSPRSSLAFVGLGALGLPMAANLLAAGYELKVHSRSRAAELNLALKGSRPCSSPA
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEEECCCCCCCHH
EAAADSQALLICVSDDEAVEAVLFGSQGAASKLSSGAVVVDFSTIAPATSITLAERLAQQ
HHCCCCCEEEEEECCCCCEEEEEECCCCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHC
GVTYLDAPVTGGTEGARAGTLTVLVGGNTQALARVQPLLEVIGESIHHFGSVGRGQQVKA
CCEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LNQVLVAGSYAALAEAIALGQQLGLPMPEVITALQHGAAGSWALQHRSTAMLEDHYPLGF
HHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEHHHCCHHHCCCCCCCCE
KLALHHKDLGIALETAERVGLQLPITSKVKSMEANLIELGHSEEDVSVLRRWFDQQQAEF
EEEEEECCCCEEEEHHHHCCEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
Q
C
>Mature Secondary Structure 
SKAQLSPRSSLAFVGLGALGLPMAANLLAAGYELKVHSRSRAAELNLALKGSRPCSSPA
CCCCCCCCCCEEEEECCHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEEECCCCCCCHH
EAAADSQALLICVSDDEAVEAVLFGSQGAASKLSSGAVVVDFSTIAPATSITLAERLAQQ
HHCCCCCEEEEEECCCCCEEEEEECCCCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHC
GVTYLDAPVTGGTEGARAGTLTVLVGGNTQALARVQPLLEVIGESIHHFGSVGRGQQVKA
CCEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LNQVLVAGSYAALAEAIALGQQLGLPMPEVITALQHGAAGSWALQHRSTAMLEDHYPLGF
HHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEHHHCCHHHCCCCCCCCE
KLALHHKDLGIALETAERVGLQLPITSKVKSMEANLIELGHSEEDVSVLRRWFDQQQAEF
EEEEEECCCCEEEEHHHHCCEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA