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The map label for this gene is folC [H]

Identifier: 124023685

GI number: 124023685

Start: 1745460

End: 1746701

Strand: Direct

Name: folC [H]

Synonym: P9303_19851

Alternate gene names: 124023685

Gene position: 1745460-1746701 (Clockwise)

Preceding gene: 124023684

Following gene: 124023686

Centisome position: 65.06

GC content: 56.28

Gene sequence:

>1242_bases
GTGGCCAAACCCACAAGTCCAAGCAAGGAAGATTTAAGCGATCTGATTCCTCGTTTTGATCAGCGGGGGATGGATCTTGG
CCTTGAGCGCATGCAACAAGCACTGCAGGCCATGGGAAACCCCTGTGCCTCTATTCCAGCCATTCAGGTCGTAGGGACCA
ATGGCAAAGGGTCTATCGCCAGCTTCATTGCCAGCAGCCTTAAGGCTGCTGGCATTCGCGTGGGGCTGACCACCTCTCCT
CACCTCGTGAGCTGGTGTGAACGGATCAGCAGCGATGGTGAGCTGATCTCAATCGTTGAGCTACGCCAACGACTCACAGC
CCTGCAAGCCCTTGCCCAAACCCACCGACTCACCCCCTTCGAGCTGTTGATGGCCACGGCTTTTGATCACTTCAGATCCC
GCGAGGTAGAGCTGCTTGTGCTTGAGGTGGGCCTCGGAGGACGCCTCGATGCCACCACAGCTCATCCATGCAGACCAATC
ATTGCCATGGCCAACATCGGCCTCGACCACTGCGAACACCTTGGTTACAGCCTTAAAGAGATAACAGCAGAAAAAAGTGC
CGTCATCAGTCCTGGGGCTGCTGTCATCAGTGCTCGTCAACAAGCTGAAGTGGCGAGCATTCTTGAAGACAAGGCAAAAC
ATCAACAGGCCCGCTTGCAATGGGTTTCACCCCTCCCAGATGACTGGACCTTGGGCTTGCCAGGAGTCCTTCAGCGGCAG
AACGCGGCGGTAGCCAAGGGTGCTCTTGAATCCCTGGTCCCACTTGGCTGGCGGCTTAATGAAGACGTCATTCGCACAGG
GCTGGCCCACGCCTACTGGCCTGCACGGCTGCAGACCGTTCACTGGCAAAGCCAGCCAGTGCTCATTGATGGTGCCCATA
ACCCACCAGCCACCGAACGGTTGGCCCACGAGCGGCAGCAGTGGAGCAATCAAGAGTTTGGGGTTTGCTGGGTGCTTGGT
CTACAGGCCCACAAGCAAGCACCAGCCATGCTTCGCCATTTACTCAAGCCAAGCGATCTGGCTTGGATCGTGCCGGTGCC
GGAACACTGCAGCTGGACGCAGCACCAGCTCGCAGCACACTGCCCGGACCTATCAAGCCAACTCCAATCTGCTGACAATG
TTGAACAGGTCTTCTCCATCCTGCTCAAACAGAATCGATGGCCAAATCCTCCTCCAGTTGTAGCTGGTTCCCTCTATTTA
TTAGGTGATCTTCTCGCAAAGCAAACCATCAAGGCAGAGTGA

Upstream 100 bases:

>100_bases
CCCAAAGGTTGTGCGGATGGTGCCGCCGCTGATCATCAAACCAAGCGAAATCCGCCAACTGCTCCAACGTCTGGAAGCCA
CCCTGGCGCACTTCCGCTAA

Downstream 100 bases:

>100_bases
GAGGTGTTGCCGTAAAGCCGATGCCGAGCTCGTTGTCGCAACGTCAAAGCCTGATGACGTTTCTGGGCTGGATGCTATGC
CTGCTGATCGCTATCCCTGC

Product: bifunctional dihydrofolate/folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MAKPTSPSKEDLSDLIPRFDQRGMDLGLERMQQALQAMGNPCASIPAIQVVGTNGKGSIASFIASSLKAAGIRVGLTTSP
HLVSWCERISSDGELISIVELRQRLTALQALAQTHRLTPFELLMATAFDHFRSREVELLVLEVGLGGRLDATTAHPCRPI
IAMANIGLDHCEHLGYSLKEITAEKSAVISPGAAVISARQQAEVASILEDKAKHQQARLQWVSPLPDDWTLGLPGVLQRQ
NAAVAKGALESLVPLGWRLNEDVIRTGLAHAYWPARLQTVHWQSQPVLIDGAHNPPATERLAHERQQWSNQEFGVCWVLG
LQAHKQAPAMLRHLLKPSDLAWIVPVPEHCSWTQHQLAAHCPDLSSQLQSADNVEQVFSILLKQNRWPNPPPVVAGSLYL
LGDLLAKQTIKAE

Sequences:

>Translated_413_residues
MAKPTSPSKEDLSDLIPRFDQRGMDLGLERMQQALQAMGNPCASIPAIQVVGTNGKGSIASFIASSLKAAGIRVGLTTSP
HLVSWCERISSDGELISIVELRQRLTALQALAQTHRLTPFELLMATAFDHFRSREVELLVLEVGLGGRLDATTAHPCRPI
IAMANIGLDHCEHLGYSLKEITAEKSAVISPGAAVISARQQAEVASILEDKAKHQQARLQWVSPLPDDWTLGLPGVLQRQ
NAAVAKGALESLVPLGWRLNEDVIRTGLAHAYWPARLQTVHWQSQPVLIDGAHNPPATERLAHERQQWSNQEFGVCWVLG
LQAHKQAPAMLRHLLKPSDLAWIVPVPEHCSWTQHQLAAHCPDLSSQLQSADNVEQVFSILLKQNRWPNPPPVVAGSLYL
LGDLLAKQTIKAE
>Mature_412_residues
AKPTSPSKEDLSDLIPRFDQRGMDLGLERMQQALQAMGNPCASIPAIQVVGTNGKGSIASFIASSLKAAGIRVGLTTSPH
LVSWCERISSDGELISIVELRQRLTALQALAQTHRLTPFELLMATAFDHFRSREVELLVLEVGLGGRLDATTAHPCRPII
AMANIGLDHCEHLGYSLKEITAEKSAVISPGAAVISARQQAEVASILEDKAKHQQARLQWVSPLPDDWTLGLPGVLQRQN
AAVAKGALESLVPLGWRLNEDVIRTGLAHAYWPARLQTVHWQSQPVLIDGAHNPPATERLAHERQQWSNQEFGVCWVLGL
QAHKQAPAMLRHLLKPSDLAWIVPVPEHCSWTQHQLAAHCPDLSSQLQSADNVEQVFSILLKQNRWPNPPPVVAGSLYLL
GDLLAKQTIKAE

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=380, Percent_Identity=27.1052631578947, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI66932990, Length=380, Percent_Identity=27.1052631578947, Blast_Score=105, Evalue=8e-23,
Organism=Escherichia coli, GI1788654, Length=379, Percent_Identity=30.0791556728232, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71984923, Length=331, Percent_Identity=26.8882175226586, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17553150, Length=331, Percent_Identity=26.8882175226586, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17553148, Length=331, Percent_Identity=26.8882175226586, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323760, Length=423, Percent_Identity=30.4964539007092, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6324815, Length=277, Percent_Identity=29.2418772563177, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24641571, Length=274, Percent_Identity=28.8321167883212, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24581568, Length=191, Percent_Identity=32.4607329842932, Blast_Score=101, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 45218; Mature: 45087

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPTSPSKEDLSDLIPRFDQRGMDLGLERMQQALQAMGNPCASIPAIQVVGTNGKGSIA
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHH
SFIASSLKAAGIRVGLTTSPHLVSWCERISSDGELISIVELRQRLTALQALAQTHRLTPF
HHHHHHHHHCCEEEECCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCHH
ELLMATAFDHFRSREVELLVLEVGLGGRLDATTAHPCRPIIAMANIGLDHCEHLGYSLKE
HHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCHHHHHHCCCHHHH
ITAEKSAVISPGAAVISARQQAEVASILEDKAKHQQARLQWVSPLPDDWTLGLPGVLQRQ
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHC
NAAVAKGALESLVPLGWRLNEDVIRTGLAHAYWPARLQTVHWQSQPVLIDGAHNPPATER
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHEEEEECCCCEEEECCCCCCHHHH
LAHERQQWSNQEFGVCWVLGLQAHKQAPAMLRHLLKPSDLAWIVPVPEHCSWTQHQLAAH
HHHHHHHHCCCCCCEEEEEECHHHCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHH
CPDLSSQLQSADNVEQVFSILLKQNRWPNPPPVVAGSLYLLGDLLAKQTIKAE
CCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKPTSPSKEDLSDLIPRFDQRGMDLGLERMQQALQAMGNPCASIPAIQVVGTNGKGSIA
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHH
SFIASSLKAAGIRVGLTTSPHLVSWCERISSDGELISIVELRQRLTALQALAQTHRLTPF
HHHHHHHHHCCEEEECCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCHH
ELLMATAFDHFRSREVELLVLEVGLGGRLDATTAHPCRPIIAMANIGLDHCEHLGYSLKE
HHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCHHHHHHCCCHHHH
ITAEKSAVISPGAAVISARQQAEVASILEDKAKHQQARLQWVSPLPDDWTLGLPGVLQRQ
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHC
NAAVAKGALESLVPLGWRLNEDVIRTGLAHAYWPARLQTVHWQSQPVLIDGAHNPPATER
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHEEEEECCCCEEEECCCCCCHHHH
LAHERQQWSNQEFGVCWVLGLQAHKQAPAMLRHLLKPSDLAWIVPVPEHCSWTQHQLAAH
HHHHHHHHCCCCCCEEEEEECHHHCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHH
CPDLSSQLQSADNVEQVFSILLKQNRWPNPPPVVAGSLYLLGDLLAKQTIKAE
CCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]