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The map label for this gene is hisA

Identifier: 124023446

GI number: 124023446

Start: 1527066

End: 1527833

Strand: Reverse

Name: hisA

Synonym: P9303_17461

Alternate gene names: 124023446

Gene position: 1527833-1527066 (Counterclockwise)

Preceding gene: 124023448

Following gene: 124023445

Centisome position: 56.95

GC content: 55.86

Gene sequence:

>768_bases
ATGGAGATCATCCCAGCCATCGACCTACTGGACAGCGCTTGCGTGCGACTTCACCAGGGCGACTACGCGAAGGTGACTCG
CTTCAGCGAGGACCCCGTTGCTCAAGCCCTGAGCTGGCAGAAGCAGGGGGCAACTCGCCTACACCTGGTGGATCTCGATG
GCGCCAAATCTGGTGAGCCGGTCAACGATTCCTGTGTGCGAGCTATTACCTCTGCCCTCAATATTCCTGTCCAGCTCGGT
GGTGGTGTGCGCACTCTCGAACGAGCTGAAGAACTTCTCGCCTACGGCCTTGAACAAGTCATCCTTGGCACCGTGGCAAT
AGAGCAACCCCAGCTAGTGAAACAGCTTGCCCAAAGGAATCCCGGTCGGATTATCGTCGGCATCGATGCCAAAAACGGCA
AGGTGGCAACGCGAGGATGGATTAGCCAAAGCGAGGTAAATGCAACAGATTTAGCCGCCGATTTCAATGCTGCCGGCATC
GCCGCAATCATCAGCACAGACATTGCAACCGATGGAACCCTTGAAGGGCCGAACCTAGAGTCATTACGTGCAATGGCCAA
CGCCAGCAGCGTTCCATTAATCGCCTCCGGAGGCGTGGGTTGCATGGCCGATCTACTTAGTCTGCTGGCTCTTGAACCCT
ATGGAGTGAGTGGGGTGATTGTGGGAAGAGCTCTCTACGACGGCAAGGTGGATCTAAAAGAAGCAATCCGAGCCATTGGC
GATGGCCGACTTCAGGACCCACCAAGTAGCAAACCACTGATGGCATGA

Upstream 100 bases:

>100_bases
AGCTGAATCATCAAACGCGCATATTGAAGCGCGTTCGATCAGCAACTGAAGGCCCTTCGCCCTGCAGGATGGAGGTCTAA
GGCAGCGTTCTTTGCCAATC

Downstream 100 bases:

>100_bases
GGACCTTGGACTATGAATTGACCTTGCTGGAGCCGTAAATTGAATTCATTATCACTTCAGTTGTGACGCATACCGCTCCC
AAGATCAGCGCCTCAAGTGG

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MEIIPAIDLLDSACVRLHQGDYAKVTRFSEDPVAQALSWQKQGATRLHLVDLDGAKSGEPVNDSCVRAITSALNIPVQLG
GGVRTLERAEELLAYGLEQVILGTVAIEQPQLVKQLAQRNPGRIIVGIDAKNGKVATRGWISQSEVNATDLAADFNAAGI
AAIISTDIATDGTLEGPNLESLRAMANASSVPLIASGGVGCMADLLSLLALEPYGVSGVIVGRALYDGKVDLKEAIRAIG
DGRLQDPPSSKPLMA

Sequences:

>Translated_255_residues
MEIIPAIDLLDSACVRLHQGDYAKVTRFSEDPVAQALSWQKQGATRLHLVDLDGAKSGEPVNDSCVRAITSALNIPVQLG
GGVRTLERAEELLAYGLEQVILGTVAIEQPQLVKQLAQRNPGRIIVGIDAKNGKVATRGWISQSEVNATDLAADFNAAGI
AAIISTDIATDGTLEGPNLESLRAMANASSVPLIASGGVGCMADLLSLLALEPYGVSGVIVGRALYDGKVDLKEAIRAIG
DGRLQDPPSSKPLMA
>Mature_255_residues
MEIIPAIDLLDSACVRLHQGDYAKVTRFSEDPVAQALSWQKQGATRLHLVDLDGAKSGEPVNDSCVRAITSALNIPVQLG
GGVRTLERAEELLAYGLEQVILGTVAIEQPQLVKQLAQRNPGRIIVGIDAKNGKVATRGWISQSEVNATDLAADFNAAGI
AAIISTDIATDGTLEGPNLESLRAMANASSVPLIASGGVGCMADLLSLLALEPYGVSGVIVGRALYDGKVDLKEAIRAIG
DGRLQDPPSSKPLMA

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=241, Percent_Identity=38.5892116182573, Blast_Score=153, Evalue=8e-39,
Organism=Escherichia coli, GI1788336, Length=245, Percent_Identity=27.7551020408163, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_PROM3 (A2CAH8)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001017753.1
- ProteinModelPortal:   A2CAH8
- SMR:   A2CAH8
- STRING:   A2CAH8
- GeneID:   4778115
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_17461
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26672; Mature: 26672

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIIPAIDLLDSACVRLHQGDYAKVTRFSEDPVAQALSWQKQGATRLHLVDLDGAKSGEP
CCCCCHHHHHHHHHHHHCCCCCHHEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCC
VNDSCVRAITSALNIPVQLGGGVRTLERAEELLAYGLEQVILGTVAIEQPQLVKQLAQRN
CCHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
PGRIIVGIDAKNGKVATRGWISQSEVNATDLAADFNAAGIAAIISTDIATDGTLEGPNLE
CCEEEEEEECCCCEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCHH
SLRAMANASSVPLIASGGVGCMADLLSLLALEPYGVSGVIVGRALYDGKVDLKEAIRAIG
HHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHC
DGRLQDPPSSKPLMA
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MEIIPAIDLLDSACVRLHQGDYAKVTRFSEDPVAQALSWQKQGATRLHLVDLDGAKSGEP
CCCCCHHHHHHHHHHHHCCCCCHHEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCC
VNDSCVRAITSALNIPVQLGGGVRTLERAEELLAYGLEQVILGTVAIEQPQLVKQLAQRN
CCHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
PGRIIVGIDAKNGKVATRGWISQSEVNATDLAADFNAAGIAAIISTDIATDGTLEGPNLE
CCEEEEEEECCCCEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCHH
SLRAMANASSVPLIASGGVGCMADLLSLLALEPYGVSGVIVGRALYDGKVDLKEAIRAIG
HHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHC
DGRLQDPPSSKPLMA
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA