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The map label for this gene is hisH [H]

Identifier: 124022620

GI number: 124022620

Start: 825706

End: 826347

Strand: Reverse

Name: hisH [H]

Synonym: P9303_09111

Alternate gene names: 124022620

Gene position: 826347-825706 (Counterclockwise)

Preceding gene: 124022621

Following gene: 124022619

Centisome position: 30.8

GC content: 55.92

Gene sequence:

>642_bases
TTGAACACACGTCTAGGCTTAATTGATTACGGCATGGGCAATCTGCACTCTGTGCAGATTGCCTTTGAAAGGCTCCACAA
GGCTCTTCACATAGTGAGGCAGCCCGCCGACCTCAGCAGCTGTGATGCATTGATCTTGCCTGGAGTCGGTGCCTTTGATC
CCGCCATGGTGCACCTTGAGCAGACCAGATTGGTTCCAGACCTCAAGAGTTGGGTGAAAGGAGGACGACCTCTTCTAGGC
ATCTGTCTGGGCCTGCAGCTGTTGTTCGAATCGAGTGACGAAGGAAATGCCACCGGCCTAGGGTTGCTTAAGGGCCACGT
TCAACGACTGCCCTCCAATCAAGGCGAACGAATTCCCCACATGGGCTGGGCTGCTCTACAACATCGAAACGACTGCCCGC
TGCTTGACAAAGAAGATCCAGACAGCTGGATGTATTTCGTGCATTCCTATGCTGCAGTCCCCAGCCAAAACAGTGATCTT
GCGGCCATAGCGCCGTTTGGCCGAGACAACATCACAGCGATGGTCTGGAAAGGCCGACTAGGGGCCTGTCAGTTTCACCC
AGAGAAATCCGCCGCAGCCGGCGAGCGCATGCTGCGGCGCTGGCTGAAGTGGCTGGAGACCGGCGCAAAACCTGTGCCAT
GA

Upstream 100 bases:

>100_bases
CTCTGAAGCTTGATCCAACTTCGATTCCAGCCCTCCTAGATCACCATTGGCCTGATTCAATGCCACACATGGCTAAAAGG
CCAGATTGCTGAGGGTTTCT

Downstream 100 bases:

>100_bases
GTAGCCAGCTAAGGCTCTCCGGAGGGCGCAAGCTGCAGAGTCCCGCTGGCCAAGGCACTAGACCCACGACGAGTCGTGTT
CGTGAAGCAGTGATGAACGT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit 2; IGP synthase subunit hisH 2; ImGP synthase subunit hisH 2; IGPS subunit hisH 2 [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MNTRLGLIDYGMGNLHSVQIAFERLHKALHIVRQPADLSSCDALILPGVGAFDPAMVHLEQTRLVPDLKSWVKGGRPLLG
ICLGLQLLFESSDEGNATGLGLLKGHVQRLPSNQGERIPHMGWAALQHRNDCPLLDKEDPDSWMYFVHSYAAVPSQNSDL
AAIAPFGRDNITAMVWKGRLGACQFHPEKSAAAGERMLRRWLKWLETGAKPVP

Sequences:

>Translated_213_residues
MNTRLGLIDYGMGNLHSVQIAFERLHKALHIVRQPADLSSCDALILPGVGAFDPAMVHLEQTRLVPDLKSWVKGGRPLLG
ICLGLQLLFESSDEGNATGLGLLKGHVQRLPSNQGERIPHMGWAALQHRNDCPLLDKEDPDSWMYFVHSYAAVPSQNSDL
AAIAPFGRDNITAMVWKGRLGACQFHPEKSAAAGERMLRRWLKWLETGAKPVP
>Mature_213_residues
MNTRLGLIDYGMGNLHSVQIAFERLHKALHIVRQPADLSSCDALILPGVGAFDPAMVHLEQTRLVPDLKSWVKGGRPLLG
ICLGLQLLFESSDEGNATGLGLLKGHVQRLPSNQGERIPHMGWAALQHRNDCPLLDKEDPDSWMYFVHSYAAVPSQNSDL
AAIAPFGRDNITAMVWKGRLGACQFHPEKSAAAGERMLRRWLKWLETGAKPVP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=198, Percent_Identity=34.8484848484849, Blast_Score=101, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6319725, Length=212, Percent_Identity=33.4905660377358, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23502; Mature: 23502

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTRLGLIDYGMGNLHSVQIAFERLHKALHIVRQPADLSSCDALILPGVGAFDPAMVHLE
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHH
QTRLVPDLKSWVKGGRPLLGICLGLQLLFESSDEGNATGLGLLKGHVQRLPSNQGERIPH
HHHCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
MGWAALQHRNDCPLLDKEDPDSWMYFVHSYAAVPSQNSDLAAIAPFGRDNITAMVWKGRL
CCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEEECCCC
GACQFHPEKSAAAGERMLRRWLKWLETGAKPVP
CCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNTRLGLIDYGMGNLHSVQIAFERLHKALHIVRQPADLSSCDALILPGVGAFDPAMVHLE
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHH
QTRLVPDLKSWVKGGRPLLGICLGLQLLFESSDEGNATGLGLLKGHVQRLPSNQGERIPH
HHHCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
MGWAALQHRNDCPLLDKEDPDSWMYFVHSYAAVPSQNSDLAAIAPFGRDNITAMVWKGRL
CCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEEECCCC
GACQFHPEKSAAAGERMLRRWLKWLETGAKPVP
CCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]