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The map label for this gene is miaA

Identifier: 124026794

GI number: 124026794

Start: 1731361

End: 1732260

Strand: Direct

Name: miaA

Synonym: NATL1_20891

Alternate gene names: 124026794

Gene position: 1731361-1732260 (Clockwise)

Preceding gene: 124026789

Following gene: 124026795

Centisome position: 92.85

GC content: 34.33

Gene sequence:

>900_bases
ATGCAACCCAATAAGCCTCTTGTCATAGCCCTTATGGGCCCAACTGCAAGTGGTAAAACTGAACTTGCAATTGATATTGC
AAAAAAAATCAATTCTAATATTCACAATATCGATTCTCGCCAAATCTATATTGATATGGATATTGGCACCGCAAAGCCAA
CAGCAGTTCAACAAAGCCAAGTAAACCATTTCCTTATTGATGTTTGCTTGCCATCTAAACCAATCAATCTTCATGATTTT
CAATCCATAGCCCAAACATCCATTGAGAGAGATTTAGAAAAAAAGGGTTTAACGTTACTTGTAGGAGGAAGTGGCTTATA
TCTTCAAGCCTTAATTGGAGGACTTAATCCTCCAGCTGTACCTCCTCAGAAATTCTTAAGAGATCAACTTAAAAAAATCG
ATAAAACCGAACTACACAAATTATTAAAGCTTTGTGACCCTTTTTCAGCGCAGAGAATACATCCAGAAGACTCAATAAGA
ATCATTCGTGCTCTTGAAGTTTTTTATGCCACGGGAAAAATGTTTTCGAAGGAAAAAAACATGACGCCTATTCCTTGGAG
AGTGTTAGAACTTGGAGTAAATCCTGAAAATTTAATCAGTAGAATTCAACGTAGAACTGAAGAAATGTATAAAAAAGGTT
TAATAGAAGAAACAGAAGATTTGATCAATAAATACGGAAAAAATTTACAGTTGCTTAAAACTATTGGATATGGTGAAGCA
AGGTCTATGATTAATGGAAAGATTAATTATGAGGAGGCTTTAGAAATAACAATCAAACGAACTTGCCAATTAGCCAAAAG
ACAAAAAACTTGGTTTAGAAATAAGCATCATTCTAAATGGCTAAATAATGAGAATGCATTACCCGAAGCTTTAACTTCTA
TCTATGAGTTTCTAGGTTGA

Upstream 100 bases:

>100_bases
TTAACATTGGTATCAAGGAAAGTCTTAGGAAAAATTTAAAACCAAATTAAAAAATTTAATTTCATGCAATTACTGCTAAT
AAAACCCCTGAAAGACAATC

Downstream 100 bases:

>100_bases
TTAGATCATATATGATTAGAATTGCTCCAAAAGATTCTTTCCACGTTAATTAATTACTGAATGCCACCACGTCCCCGTTT
TGATCGTCACGCTCCAGAAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MQPNKPLVIALMGPTASGKTELAIDIAKKINSNIHNIDSRQIYIDMDIGTAKPTAVQQSQVNHFLIDVCLPSKPINLHDF
QSIAQTSIERDLEKKGLTLLVGGSGLYLQALIGGLNPPAVPPQKFLRDQLKKIDKTELHKLLKLCDPFSAQRIHPEDSIR
IIRALEVFYATGKMFSKEKNMTPIPWRVLELGVNPENLISRIQRRTEEMYKKGLIEETEDLINKYGKNLQLLKTIGYGEA
RSMINGKINYEEALEITIKRTCQLAKRQKTWFRNKHHSKWLNNENALPEALTSIYEFLG

Sequences:

>Translated_299_residues
MQPNKPLVIALMGPTASGKTELAIDIAKKINSNIHNIDSRQIYIDMDIGTAKPTAVQQSQVNHFLIDVCLPSKPINLHDF
QSIAQTSIERDLEKKGLTLLVGGSGLYLQALIGGLNPPAVPPQKFLRDQLKKIDKTELHKLLKLCDPFSAQRIHPEDSIR
IIRALEVFYATGKMFSKEKNMTPIPWRVLELGVNPENLISRIQRRTEEMYKKGLIEETEDLINKYGKNLQLLKTIGYGEA
RSMINGKINYEEALEITIKRTCQLAKRQKTWFRNKHHSKWLNNENALPEALTSIYEFLG
>Mature_299_residues
MQPNKPLVIALMGPTASGKTELAIDIAKKINSNIHNIDSRQIYIDMDIGTAKPTAVQQSQVNHFLIDVCLPSKPINLHDF
QSIAQTSIERDLEKKGLTLLVGGSGLYLQALIGGLNPPAVPPQKFLRDQLKKIDKTELHKLLKLCDPFSAQRIHPEDSIR
IIRALEVFYATGKMFSKEKNMTPIPWRVLELGVNPENLISRIQRRTEEMYKKGLIEETEDLINKYGKNLQLLKTIGYGEA
RSMINGKINYEEALEITIKRTCQLAKRQKTWFRNKHHSKWLNNENALPEALTSIYEFLG

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=325, Percent_Identity=27.0769230769231, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1790613, Length=291, Percent_Identity=36.7697594501718, Blast_Score=171, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI25144712, Length=258, Percent_Identity=25.5813953488372, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324848, Length=287, Percent_Identity=29.9651567944251, Blast_Score=123, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24649830, Length=262, Percent_Identity=33.206106870229, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_PROM1 (A2C585)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015909.1
- ProteinModelPortal:   A2C585
- STRING:   A2C585
- GeneID:   4780660
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_20891
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   PMAR167555:NATL1_20891-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33995; Mature: 33995

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPNKPLVIALMGPTASGKTELAIDIAKKINSNIHNIDSRQIYIDMDIGTAKPTAVQQSQ
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHH
VNHFLIDVCLPSKPINLHDFQSIAQTSIERDLEKKGLTLLVGGSGLYLQALIGGLNPPAV
HHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCCCCCC
PPQKFLRDQLKKIDKTELHKLLKLCDPFSAQRIHPEDSIRIIRALEVFYATGKMFSKEKN
CHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
MTPIPWRVLELGVNPENLISRIQRRTEEMYKKGLIEETEDLINKYGKNLQLLKTIGYGEA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEHHHHCCHHH
RSMINGKINYEEALEITIKRTCQLAKRQKTWFRNKHHSKWLNNENALPEALTSIYEFLG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MQPNKPLVIALMGPTASGKTELAIDIAKKINSNIHNIDSRQIYIDMDIGTAKPTAVQQSQ
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHH
VNHFLIDVCLPSKPINLHDFQSIAQTSIERDLEKKGLTLLVGGSGLYLQALIGGLNPPAV
HHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCCCCCC
PPQKFLRDQLKKIDKTELHKLLKLCDPFSAQRIHPEDSIRIIRALEVFYATGKMFSKEKN
CHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
MTPIPWRVLELGVNPENLISRIQRRTEEMYKKGLIEETEDLINKYGKNLQLLKTIGYGEA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEHHHHCCHHH
RSMINGKINYEEALEITIKRTCQLAKRQKTWFRNKHHSKWLNNENALPEALTSIYEFLG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA