Click here to switch to the map view.

The map label for this gene is prsA [H]

Identifier: 124026209

GI number: 124026209

Start: 1214887

End: 1215882

Strand: Direct

Name: prsA [H]

Synonym: NATL1_15031

Alternate gene names: 124026209

Gene position: 1214887-1215882 (Clockwise)

Preceding gene: 124026206

Following gene: 124026213

Centisome position: 65.15

GC content: 40.96

Gene sequence:

>996_bases
GTGACCAGTTTTATTACTGCAGCGAATCCTGAGCCTACTAGTCTCGAGCATGACACTAATAGACTCAGATTAATTAGTGG
GACATCCAATACAGCATTAGCTAAAGAAATTGCGACTTATATGGGGATAACCAATGTCCCTCTAGTTTCAAAAAGGTTCG
CAGATGGAGAGCTCTATGTGCAAATCCAGCAATCAATTAGAGGATGTGATGTTTTTCTAATACAACCAACCTGTGCTCCT
GTTAATGACAGCTTAATGGAGCTAATGATCATGGTGGATGCTTGTAAAAGAGCATCTGCCAGACAAATTACAGCTGTAAT
TCCTTATTTTGGTTACGCCAGAGCAGACAGAAAGACAGCTGGTAGAGAGTCAATAACTGCGAAGCTAACCGCAAATATTC
TTGTGAAATCAGGAGTAGATAGAGTACTCGCAATGGACTTACATTCCGCACAAATCCAAGGTTATTTCGATATCCCGTGC
GATCACATATATGGTTCACCTGTTCTAGTTGATTATCTATCAACTATGAAGCTTGATGAAATAGTAGTCGTCTCGCCGGA
TGTAGGTGGAGTAGCCAGAGCCAGAGCTTTTGCTAAGCAAATGAAGGACTCACCTCTAGCCATTATTGATAAACGTCGCT
CAGGGCATAATGTTGCCGAAAGCCTTACAGTGATTGGGGAAGTCTCTGGCAAAACGGCAATATTGATTGATGATATGATT
GACACTGGAGGTACTATTTGCGCTGGAGCTGAATTACTTAGGAAGGAGGGTGCTAAAAAAGTTATTGCATGTGCATCTCA
TGCTGTTTTCTCTCCACCTGCTTACGCGAGGCTTTCAAAAGAAGGCCTTTTTGAACAAGTTATTGTTACTAATAGTATTC
CGGTGCCAAGTAATTTAGATTTCTCACAATTGAAGGTCTTATCAGTTGCAAATATGCTTGGAGAAGCTATTTGGAGAATT
CATGAAGAAAGTTCTGTTAGCTCAATGTTCAGATGA

Upstream 100 bases:

>100_bases
AATAAAGTTTTAAAAGAAAAAATATAAAAAAGCTTTTATACGATCATGTCTCAACATAACGGTAGATTAGTAAATATCAT
TTGTCGCTTGCTGTGGAACC

Downstream 100 bases:

>100_bases
ATTTTTAAATTTAATTCAAAATCAGTTCCACTACCTCTCTTGTATTAGAAGATATATTTGCTTTGTTTAATTTTAATAGT
GATTCATAAATCCCTTCCTT

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTSFITAANPEPTSLEHDTNRLRLISGTSNTALAKEIATYMGITNVPLVSKRFADGELYVQIQQSIRGCDVFLIQPTCAP
VNDSLMELMIMVDACKRASARQITAVIPYFGYARADRKTAGRESITAKLTANILVKSGVDRVLAMDLHSAQIQGYFDIPC
DHIYGSPVLVDYLSTMKLDEIVVVSPDVGGVARARAFAKQMKDSPLAIIDKRRSGHNVAESLTVIGEVSGKTAILIDDMI
DTGGTICAGAELLRKEGAKKVIACASHAVFSPPAYARLSKEGLFEQVIVTNSIPVPSNLDFSQLKVLSVANMLGEAIWRI
HEESSVSSMFR

Sequences:

>Translated_331_residues
MTSFITAANPEPTSLEHDTNRLRLISGTSNTALAKEIATYMGITNVPLVSKRFADGELYVQIQQSIRGCDVFLIQPTCAP
VNDSLMELMIMVDACKRASARQITAVIPYFGYARADRKTAGRESITAKLTANILVKSGVDRVLAMDLHSAQIQGYFDIPC
DHIYGSPVLVDYLSTMKLDEIVVVSPDVGGVARARAFAKQMKDSPLAIIDKRRSGHNVAESLTVIGEVSGKTAILIDDMI
DTGGTICAGAELLRKEGAKKVIACASHAVFSPPAYARLSKEGLFEQVIVTNSIPVPSNLDFSQLKVLSVANMLGEAIWRI
HEESSVSSMFR
>Mature_330_residues
TSFITAANPEPTSLEHDTNRLRLISGTSNTALAKEIATYMGITNVPLVSKRFADGELYVQIQQSIRGCDVFLIQPTCAPV
NDSLMELMIMVDACKRASARQITAVIPYFGYARADRKTAGRESITAKLTANILVKSGVDRVLAMDLHSAQIQGYFDIPCD
HIYGSPVLVDYLSTMKLDEIVVVSPDVGGVARARAFAKQMKDSPLAIIDKRRSGHNVAESLTVIGEVSGKTAILIDDMID
TGGTICAGAELLRKEGAKKVIACASHAVFSPPAYARLSKEGLFEQVIVTNSIPVPSNLDFSQLKVLSVANMLGEAIWRIH
EESSVSSMFR

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI28557709, Length=312, Percent_Identity=41.6666666666667, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI4506129, Length=310, Percent_Identity=41.6129032258065, Blast_Score=258, Evalue=4e-69,
Organism=Homo sapiens, GI4506127, Length=312, Percent_Identity=41.3461538461538, Blast_Score=258, Evalue=5e-69,
Organism=Homo sapiens, GI84875539, Length=313, Percent_Identity=41.5335463258786, Blast_Score=253, Evalue=1e-67,
Organism=Homo sapiens, GI4506133, Length=360, Percent_Identity=35, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI194018537, Length=347, Percent_Identity=34.8703170028818, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI310128524, Length=144, Percent_Identity=29.1666666666667, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI310115209, Length=144, Percent_Identity=29.1666666666667, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI310118259, Length=144, Percent_Identity=29.1666666666667, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI310119946, Length=144, Percent_Identity=29.1666666666667, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1787458, Length=311, Percent_Identity=48.8745980707396, Blast_Score=315, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17554702, Length=312, Percent_Identity=42.3076923076923, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI25149168, Length=312, Percent_Identity=42.3076923076923, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI71989924, Length=312, Percent_Identity=42.3076923076923, Blast_Score=248, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI17554704, Length=310, Percent_Identity=42.5806451612903, Blast_Score=247, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI17570245, Length=338, Percent_Identity=31.6568047337278, Blast_Score=161, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6320946, Length=315, Percent_Identity=40.6349206349206, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6319403, Length=315, Percent_Identity=39.6825396825397, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6321776, Length=316, Percent_Identity=38.6075949367089, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6322667, Length=197, Percent_Identity=40.1015228426396, Blast_Score=153, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6324511, Length=115, Percent_Identity=33.9130434782609, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21355239, Length=312, Percent_Identity=41.9871794871795, Blast_Score=252, Evalue=2e-67,
Organism=Drosophila melanogaster, GI45551540, Length=335, Percent_Identity=39.7014925373134, Blast_Score=239, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24651458, Length=358, Percent_Identity=32.4022346368715, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24651456, Length=358, Percent_Identity=32.4022346368715, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI281362873, Length=358, Percent_Identity=32.4022346368715, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24651454, Length=358, Percent_Identity=32.4022346368715, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24651462, Length=186, Percent_Identity=36.5591397849462, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24651464, Length=186, Percent_Identity=36.5591397849462, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI45552010, Length=186, Percent_Identity=36.5591397849462, Blast_Score=124, Evalue=6e-29,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 35838; Mature: 35707

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSFITAANPEPTSLEHDTNRLRLISGTSNTALAKEIATYMGITNVPLVSKRFADGELYV
CCCEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHCCCCEEEE
QIQQSIRGCDVFLIQPTCAPVNDSLMELMIMVDACKRASARQITAVIPYFGYARADRKTA
EEEHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHEEEEEECCCCCHHCCHHCC
GRESITAKLTANILVKSGVDRVLAMDLHSAQIQGYFDIPCDHIYGSPVLVDYLSTMKLDE
CCHHEEHHHHHHHHHHCCCCCEEHHHHHHHHCCEEEECCHHHHCCCHHHHHHHHHCCCCE
IVVVSPDVGGVARARAFAKQMKDSPLAIIDKRRSGHNVAESLTVIGEVSGKTAILIDDMI
EEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHEEECCCCEEEEEECHH
DTGGTICAGAELLRKEGAKKVIACASHAVFSPPAYARLSKEGLFEQVIVTNSIPVPSNLD
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCCCCC
FSQLKVLSVANMLGEAIWRIHEESSVSSMFR
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC
>Mature Secondary Structure 
TSFITAANPEPTSLEHDTNRLRLISGTSNTALAKEIATYMGITNVPLVSKRFADGELYV
CCEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHCCCCEEEE
QIQQSIRGCDVFLIQPTCAPVNDSLMELMIMVDACKRASARQITAVIPYFGYARADRKTA
EEEHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHEEEEEECCCCCHHCCHHCC
GRESITAKLTANILVKSGVDRVLAMDLHSAQIQGYFDIPCDHIYGSPVLVDYLSTMKLDE
CCHHEEHHHHHHHHHHCCCCCEEHHHHHHHHCCEEEECCHHHHCCCHHHHHHHHHCCCCE
IVVVSPDVGGVARARAFAKQMKDSPLAIIDKRRSGHNVAESLTVIGEVSGKTAILIDDMI
EEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHEEECCCCEEEEEECHH
DTGGTICAGAELLRKEGAKKVIACASHAVFSPPAYARLSKEGLFEQVIVTNSIPVPSNLD
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCCCCC
FSQLKVLSVANMLGEAIWRIHEESSVSSMFR
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]