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The map label for this gene is cobI [H]

Identifier: 124025153

GI number: 124025153

Start: 403538

End: 404266

Strand: Direct

Name: cobI [H]

Synonym: NATL1_04401

Alternate gene names: 124025153

Gene position: 403538-404266 (Clockwise)

Preceding gene: 124025152

Following gene: 124025156

Centisome position: 21.64

GC content: 37.86

Gene sequence:

>729_bases
ATGAAAGTTTTGACTATTGCTGGCGTAGGGCCAGGTGATCCTTCTTTATTAACTTTGGCAGCAGTTGATGCCATTCAAGA
ATCAACAGTTGTTTCTTATCCAGTCTCTACGAGAGGAGGAGCTAGTCTTGCTGAAAAAATTGCTTCAAAATGGATAACCA
AAGATAAAAAAAAATTACCTTTACATTTTCCCATGGTTGACGATCAGAACACTTTAAAAAGTGCATGGCGAGTTGCTGGG
AGAGAATTAATGAAGATGGTTGACAAAGGTGAAAGAGTTGTTTTCCTTGCTCAAGGAGATATCTCGCTTTTCTCGACAGG
TTCTTATCTTTCAAAGGAGTTAGAAAAATATCATCCAGAGTGCGTTGTTAAATTAATTCCAGGTGTGACATCTTTTTCTG
CAGCCGCTGCAAAAAGTAAATTACCACTTGCTTTTCAAGAGGAAGAATTACTTGTATTACCTGTTCCAGACTCATATGAC
GAGCTAAAGTCTATTTTGTCTGATGCAGAGTCAAAGAAAAGGGTAGTTGTTTTGCTCAAGCTTGGTAAAAAATGGGAATG
GGTCAAACTTTTGCTTGAAGAACTTGATCTAATAAAAATTTCTATATTCGCAGAAAGAATAGGATTTTCAGATCAACAAA
TACTTAGGGCATCTGACCTACCCTCAGGGACTAGGCCATATTTTTCTTTACTGCTGATTCGACAAAGTTGGCCTTTGACA
ATGCCCTAA

Upstream 100 bases:

>100_bases
ATTAGCTAAGGTTGAATCTGATGCTTTATTGGAAATGCATCATCTGCCAAGGATGCATAGTTAATTATCTCTTTTAAGTA
GACAGAAGTTACCCAATCAA

Downstream 100 bases:

>100_bases
AACTTTTTAGTTGAATTTAATCAGTTCGTCTTCCAGTAGTTTAATTGCGTCGTTTGGAGTGCTTGCTCTAACTAATTTAT
GGCGAAGAGTAGACGCACCC

Product: precorrin-2 C20-methyltransferase

Products: NA

Alternate protein names: S-adenosyl-L-methionine--cobalt-precorrin-2 methyltransferase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MKVLTIAGVGPGDPSLLTLAAVDAIQESTVVSYPVSTRGGASLAEKIASKWITKDKKKLPLHFPMVDDQNTLKSAWRVAG
RELMKMVDKGERVVFLAQGDISLFSTGSYLSKELEKYHPECVVKLIPGVTSFSAAAAKSKLPLAFQEEELLVLPVPDSYD
ELKSILSDAESKKRVVVLLKLGKKWEWVKLLLEELDLIKISIFAERIGFSDQQILRASDLPSGTRPYFSLLLIRQSWPLT
MP

Sequences:

>Translated_242_residues
MKVLTIAGVGPGDPSLLTLAAVDAIQESTVVSYPVSTRGGASLAEKIASKWITKDKKKLPLHFPMVDDQNTLKSAWRVAG
RELMKMVDKGERVVFLAQGDISLFSTGSYLSKELEKYHPECVVKLIPGVTSFSAAAAKSKLPLAFQEEELLVLPVPDSYD
ELKSILSDAESKKRVVVLLKLGKKWEWVKLLLEELDLIKISIFAERIGFSDQQILRASDLPSGTRPYFSLLLIRQSWPLT
MP
>Mature_242_residues
MKVLTIAGVGPGDPSLLTLAAVDAIQESTVVSYPVSTRGGASLAEKIASKWITKDKKKLPLHFPMVDDQNTLKSAWRVAG
RELMKMVDKGERVVFLAQGDISLFSTGSYLSKELEKYHPECVVKLIPGVTSFSAAAAKSKLPLAFQEEELLVLPVPDSYD
ELKSILSDAESKKRVVVLLKLGKKWEWVKLLLEELDLIKISIFAERIGFSDQQILRASDLPSGTRPYFSLLLIRQSWPLT
MP

Specific function: Methylates cobalt-precorrin-2 at the C-20 position to produce cobalt-precorrin-3A in the anaerobic cobalamin biosynthesis pathway [H]

COG id: COG2243

COG function: function code H; Precorrin-2 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR012382
- InterPro:   IPR006364
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.151 [H]

Molecular weight: Translated: 26799; Mature: 26799

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLTIAGVGPGDPSLLTLAAVDAIQESTVVSYPVSTRGGASLAEKIASKWITKDKKKLP
CEEEEEECCCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCC
LHFPMVDDQNTLKSAWRVAGRELMKMVDKGERVVFLAQGDISLFSTGSYLSKELEKYHPE
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEECCHHHHHHHHHHCHH
CVVKLIPGVTSFSAAAAKSKLPLAFQEEELLVLPVPDSYDELKSILSDAESKKRVVVLLK
HHHHHHCCCCHHHHHHHHCCCCEEECCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEE
LGKKWEWVKLLLEELDLIKISIFAERIGFSDQQILRASDLPSGTRPYFSLLLIRQSWPLT
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHEEECCCCCCC
MP
CC
>Mature Secondary Structure
MKVLTIAGVGPGDPSLLTLAAVDAIQESTVVSYPVSTRGGASLAEKIASKWITKDKKKLP
CEEEEEECCCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCC
LHFPMVDDQNTLKSAWRVAGRELMKMVDKGERVVFLAQGDISLFSTGSYLSKELEKYHPE
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEECCHHHHHHHHHHCHH
CVVKLIPGVTSFSAAAAKSKLPLAFQEEELLVLPVPDSYDELKSILSDAESKKRVVVLLK
HHHHHHCCCCHHHHHHHHCCCCEEECCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEE
LGKKWEWVKLLLEELDLIKISIFAERIGFSDQQILRASDLPSGTRPYFSLLLIRQSWPLT
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHEEECCCCCCC
MP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]