Click here to switch to the map view.

The map label for this gene is amtB [C]

Identifier: 124025054

GI number: 124025054

Start: 315399

End: 316889

Strand: Direct

Name: amtB [C]

Synonym: NATL1_03411

Alternate gene names: 124025054

Gene position: 315399-316889 (Clockwise)

Preceding gene: 124025053

Following gene: 124025055

Centisome position: 16.91

GC content: 45.07

Gene sequence:

>1491_bases
ATGACCACTGCTTTGCAATCGCCTCCAAGGCGAACTACTTCTCGTCTCCAAGATGCAAGTCTCTTGAACGGGCCAATGCT
TCTTCTCAGAAGTATTAAAGGCTTTAGAAGGAGTCAGTCTTGGGCATGGCTAGCCTCTATCCCATTGGCCCTTTTGGGAT
TAGGTGTTTTCACTTTCTCTGCAAGAGCTGAGGTTGCTCTTTCAGATTTAACAGGACCACAAGCCGCTTCTTTCTTGGCT
GACAACCTTTGGTTATTTATAGCCACAATCCTCGTTATTTTTATGAACGCGGGATTCGCAATGGTTGAAGCTGGTATGTG
CCGTCAAAAAAATGCGGTCAACATTCTAGCTAAAAATTTATTTGTTTTTGCTCTTGCTGTTACTGCCTACTGGGTAATTG
GTTATTCATTAATGTATGGCGGTTCAGTTATTGATGGATGGCTTTATTTCCAAGGCTTATTCGTTGATCCTGACCCTTCA
GGTGCACTTGAGTGTGCAGCCGCAGGAGATACAGGTTGTCTTGTCCCAGCAGTTGATTTCCTTTTCCAATCTGCTTTTGC
CGGCACTGCTGCAACGATCGTTTCGGGATTGGTTGCTGAGAGAGTCAAATTCGGTGAATTTGTAGTTTTCTCTATAGTTT
TGACTGCTTTTATCTATCCAATTGCTGGAAGTTGGCAATGGAACGGTGGTTGGCTTGCTGAACTTGGTTTTATTGATTTC
GCTGGTTCATCCATTGTCCACTCTGTTGGAGGATGGGCAGGTCTTGTTGGAGCAATGCTTCTTGGACCTCGTATTGGCAA
ATTTGTTGATGGCAAAGCTCAAGCGATGCCTGGACACAATATGGCCATTGCAACTTTAGGAGCACTAATCCTTTGGATTG
GTTGGTATGGATTCAACCCTGGCTCAGAATTAGCTATGGATCAATATGTTGCTTACGTTGCAGTTACAACAACTTTGGCA
GCAGCTGGTGGTGCAATCGCCGCTACAGTTCTATCTACCATTACTTCTGGTAAGCCTGATCTAACCATGATCATTAATGG
CATTCTTGCTGGATTAGTAAGTATCACTGCTGGTTGTGGCAATATGACTTTTGCTGGATCTTGGCTTGCTGGTGCTGTAG
GTGGATTAATCGTCGTAGTTGCAGTAGCTGCTCTGGATTCTGCAGGAATTGATGATCCAGTTGGTGCTTTCTCAGTTCAC
GGTGTTTGTGGAGTTTGGGGAACTGTTGTTATCGGCCTTTGGGGCGTTGATGGCATGGATCCTGGAGCTGCAGGAATTGG
TCTTCTCAATGGAGGAGGAATTAACCAATTCTTCATTCAAGCATTAGGTGCAGCTGCTTATGGCATATGGACTGTTGTGA
CATGCTGGATTGCTTGGCAAATTATTGGTGGCTTCTTCGGAGGTATCAGAGTTAGCGAAGCAGAAGAGACACAGGGACTT
GATATTGGCGAGCATGGAATGGAGGCTTATCCTGACTTTGCTTCTAGCTAG

Upstream 100 bases:

>100_bases
TCAACACTGACATCTTGTAAGTATAAGTTGCGTACATATTTAATATTGTATAAATTTTGGATTAACTATCCATTTCTTTT
TCTCGAAGTAAGCATCATTT

Downstream 100 bases:

>100_bases
TTAACTTGTTTAATTGTCTTACATTAAATCTAAAAAAGCCCAGAATATATCTGGGCTTTTTTATTGTGGTTTAATAGTGA
TGAGATTCCTATCAATTTGT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MTTALQSPPRRTTSRLQDASLLNGPMLLLRSIKGFRRSQSWAWLASIPLALLGLGVFTFSARAEVALSDLTGPQAASFLA
DNLWLFIATILVIFMNAGFAMVEAGMCRQKNAVNILAKNLFVFALAVTAYWVIGYSLMYGGSVIDGWLYFQGLFVDPDPS
GALECAAAGDTGCLVPAVDFLFQSAFAGTAATIVSGLVAERVKFGEFVVFSIVLTAFIYPIAGSWQWNGGWLAELGFIDF
AGSSIVHSVGGWAGLVGAMLLGPRIGKFVDGKAQAMPGHNMAIATLGALILWIGWYGFNPGSELAMDQYVAYVAVTTTLA
AAGGAIAATVLSTITSGKPDLTMIINGILAGLVSITAGCGNMTFAGSWLAGAVGGLIVVVAVAALDSAGIDDPVGAFSVH
GVCGVWGTVVIGLWGVDGMDPGAAGIGLLNGGGINQFFIQALGAAAYGIWTVVTCWIAWQIIGGFFGGIRVSEAEETQGL
DIGEHGMEAYPDFASS

Sequences:

>Translated_496_residues
MTTALQSPPRRTTSRLQDASLLNGPMLLLRSIKGFRRSQSWAWLASIPLALLGLGVFTFSARAEVALSDLTGPQAASFLA
DNLWLFIATILVIFMNAGFAMVEAGMCRQKNAVNILAKNLFVFALAVTAYWVIGYSLMYGGSVIDGWLYFQGLFVDPDPS
GALECAAAGDTGCLVPAVDFLFQSAFAGTAATIVSGLVAERVKFGEFVVFSIVLTAFIYPIAGSWQWNGGWLAELGFIDF
AGSSIVHSVGGWAGLVGAMLLGPRIGKFVDGKAQAMPGHNMAIATLGALILWIGWYGFNPGSELAMDQYVAYVAVTTTLA
AAGGAIAATVLSTITSGKPDLTMIINGILAGLVSITAGCGNMTFAGSWLAGAVGGLIVVVAVAALDSAGIDDPVGAFSVH
GVCGVWGTVVIGLWGVDGMDPGAAGIGLLNGGGINQFFIQALGAAAYGIWTVVTCWIAWQIIGGFFGGIRVSEAEETQGL
DIGEHGMEAYPDFASS
>Mature_495_residues
TTALQSPPRRTTSRLQDASLLNGPMLLLRSIKGFRRSQSWAWLASIPLALLGLGVFTFSARAEVALSDLTGPQAASFLAD
NLWLFIATILVIFMNAGFAMVEAGMCRQKNAVNILAKNLFVFALAVTAYWVIGYSLMYGGSVIDGWLYFQGLFVDPDPSG
ALECAAAGDTGCLVPAVDFLFQSAFAGTAATIVSGLVAERVKFGEFVVFSIVLTAFIYPIAGSWQWNGGWLAELGFIDFA
GSSIVHSVGGWAGLVGAMLLGPRIGKFVDGKAQAMPGHNMAIATLGALILWIGWYGFNPGSELAMDQYVAYVAVTTTLAA
AGGAIAATVLSTITSGKPDLTMIINGILAGLVSITAGCGNMTFAGSWLAGAVGGLIVVVAVAALDSAGIDDPVGAFSVHG
VCGVWGTVVIGLWGVDGMDPGAAGIGLLNGGGINQFFIQALGAAAYGIWTVVTCWIAWQIIGGFFGGIRVSEAEETQGLD
IGEHGMEAYPDFASS

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=419, Percent_Identity=34.1288782816229, Blast_Score=182, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17550182, Length=436, Percent_Identity=35.5504587155963, Blast_Score=194, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI133931100, Length=428, Percent_Identity=32.0093457943925, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI32564042, Length=425, Percent_Identity=31.5294117647059, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17550180, Length=467, Percent_Identity=32.3340471092077, Blast_Score=181, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6324187, Length=424, Percent_Identity=28.7735849056604, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6321559, Length=408, Percent_Identity=28.4313725490196, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325396, Length=409, Percent_Identity=27.8728606356968, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161078314, Length=419, Percent_Identity=36.2768496420048, Blast_Score=219, Evalue=4e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 51432; Mature: 51301

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTALQSPPRRTTSRLQDASLLNGPMLLLRSIKGFRRSQSWAWLASIPLALLGLGVFTFS
CCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
ARAEVALSDLTGPQAASFLADNLWLFIATILVIFMNAGFAMVEAGMCRQKNAVNILAKNL
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHH
FVFALAVTAYWVIGYSLMYGGSVIDGWLYFQGLFVDPDPSGALECAAAGDTGCLVPAVDF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEEEECCCCCCCEEEECCCCCCCHHHHHHH
LFQSAFAGTAATIVSGLVAERVKFGEFVVFSIVLTAFIYPIAGSWQWNGGWLAELGFIDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEECCHHH
AGSSIVHSVGGWAGLVGAMLLGPRIGKFVDGKAQAMPGHNMAIATLGALILWIGWYGFNP
CCHHHHHHHCHHHHHHHHHHHCCHHCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
GSELAMDQYVAYVAVTTTLAAAGGAIAATVLSTITSGKPDLTMIINGILAGLVSITAGCG
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
NMTFAGSWLAGAVGGLIVVVAVAALDSAGIDDPVGAFSVHGVCGVWGTVVIGLWGVDGMD
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCC
PGAAGIGLLNGGGINQFFIQALGAAAYGIWTVVTCWIAWQIIGGFFGGIRVSEAEETQGL
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHCCCC
DIGEHGMEAYPDFASS
CCCCCCHHHCCCCCCC
>Mature Secondary Structure 
TTALQSPPRRTTSRLQDASLLNGPMLLLRSIKGFRRSQSWAWLASIPLALLGLGVFTFS
CCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
ARAEVALSDLTGPQAASFLADNLWLFIATILVIFMNAGFAMVEAGMCRQKNAVNILAKNL
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHH
FVFALAVTAYWVIGYSLMYGGSVIDGWLYFQGLFVDPDPSGALECAAAGDTGCLVPAVDF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEEEECCCCCCCEEEECCCCCCCHHHHHHH
LFQSAFAGTAATIVSGLVAERVKFGEFVVFSIVLTAFIYPIAGSWQWNGGWLAELGFIDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEECCHHH
AGSSIVHSVGGWAGLVGAMLLGPRIGKFVDGKAQAMPGHNMAIATLGALILWIGWYGFNP
CCHHHHHHHCHHHHHHHHHHHCCHHCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
GSELAMDQYVAYVAVTTTLAAAGGAIAATVLSTITSGKPDLTMIINGILAGLVSITAGCG
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
NMTFAGSWLAGAVGGLIVVVAVAALDSAGIDDPVGAFSVHGVCGVWGTVVIGLWGVDGMD
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCC
PGAAGIGLLNGGGINQFFIQALGAAAYGIWTVVTCWIAWQIIGGFFGGIRVSEAEETQGL
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHCCCC
DIGEHGMEAYPDFASS
CCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]