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The map label for this gene is trmE

Identifier: 124024977

GI number: 124024977

Start: 242683

End: 244077

Strand: Reverse

Name: trmE

Synonym: NATL1_02641

Alternate gene names: 124024977

Gene position: 244077-242683 (Counterclockwise)

Preceding gene: 124024980

Following gene: 124024976

Centisome position: 13.09

GC content: 35.34

Gene sequence:

>1395_bases
ATGAATTCGTTTTCCCCTACTGAAGATACTATTGCTGCAATTGCAACTGCCGTTTCTCCCGGCCAAGGAAGTATTGCTGC
CATTAGGATATCTGGCTCCTCTGCAATTGAGACCAGTAAGAATATTGTTGATGTTCCGGGAATTCAGGATTGGAGCACTC
ACAAAGTGCTTTACGGTCATGTAACTGAAGAAAATCGAAAAAAATATATTGATGAAGTTTTAATTCTCGTAATGAAAGGG
CCGAGAAGCTTTACAGGTGAAGATGTAGTAGAAATTCATTGCCATGGTGGAATTATTCCAGTCCAGAAGATCCTTGAAAG
AATACTAGCTTTCCCCAGCGTGCGAAGAGCAGAACCAGGTGAGTTTAGTCAACGAGCAGTGCTTAATGGTCGTCTTAGCC
TGACTCAAGCAGAATCAATTAGTGAGCTGGTGTCAGCAAGGAGTCGAAAAGCAGCAGAACTCGCAATTAATGGAATTGAA
GGAAATATTCAAACTACGATTCAATCAATCAGAAAGCGATTAATAGAGCAACTAACGGAAATTGAAGCAAGAATAGATTT
TGAAGAAGATCTACCACTTTTAGATGAAAAACATGTAAAGAATGAAATTGTCGCAATTAAAAAAGATCTTAACGAACTAA
TTGATAATGCTAAAAGAGGATCTTGGGTTCGTTCTGGCTTAAAAGTTGCACTAGCTGGGAAGCCTAATGTAGGGAAAAGC
TCCTTAATGAATAGGCTCTCCAAACAAGAGAAGGCCATTGTGACTGACCTACCTGGCACCACTAGAGATATTTTGGAAAG
TGAGATTGTCTTAGAGGGAATACCAGTAACTTTTATTGATACAGCAGGTCTAAGAGACACCAAAGACATCATCGAAAAAA
TTGGTATATCTAGAACTAAAAAAACTTTAATTCATGCTGATCTTATTATATTAATCTTTGATTATTCAAGCGGATGGACT
AACGAGGATGAATCAATACTAAAACAATTACCCGTAAATATTCCACTCTTGATTGTTGGTAATAAATCTGATTTAATGAA
TGATCAATCTTTTGAAAAAGTTCCAAAATATATATTAAAAAAAGAAAATCTAGTTATTTTAAGTGCAAAAACAGGAAATG
GAGAAGACGATCTAATTAATTACCTACTAAAAAAATGTGGTTCTTCTCAAACGCATGGATTGGATATTGCTCTAAATGAA
AGACAACTTGATCTAGCAAAATCAACGACGGAATCCCTAGAAAATATTAATAAAGTTTTTGACGAAAAGCTACCATGGGA
TTTCTGGACGATAGATTTAAGACAAGCTATTAATTATTTAGGAGAACTAACAGGAGAGGATTTAACAGAAAATTTACTTG
ATAATATATTTTCAAAATTCTGTATTGGTAAGTAA

Upstream 100 bases:

>100_bases
TTGTATTAGATTTAGAAAGATTTTTTTCATATTTTAAAAAATCTTGGTCCAAAAAGAAAACTAAATTTGATCTTAGTGAA
GATTGCGTGATTATTGATTA

Downstream 100 bases:

>100_bases
TCAAGCAATTTTTATCTACTAGTTATTGTCGATTGTAAAAATATAATATAAGTAATAACAAACCCCTCCAATTAACATCG
CCACCATAATATTCACACTA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MNSFSPTEDTIAAIATAVSPGQGSIAAIRISGSSAIETSKNIVDVPGIQDWSTHKVLYGHVTEENRKKYIDEVLILVMKG
PRSFTGEDVVEIHCHGGIIPVQKILERILAFPSVRRAEPGEFSQRAVLNGRLSLTQAESISELVSARSRKAAELAINGIE
GNIQTTIQSIRKRLIEQLTEIEARIDFEEDLPLLDEKHVKNEIVAIKKDLNELIDNAKRGSWVRSGLKVALAGKPNVGKS
SLMNRLSKQEKAIVTDLPGTTRDILESEIVLEGIPVTFIDTAGLRDTKDIIEKIGISRTKKTLIHADLIILIFDYSSGWT
NEDESILKQLPVNIPLLIVGNKSDLMNDQSFEKVPKYILKKENLVILSAKTGNGEDDLINYLLKKCGSSQTHGLDIALNE
RQLDLAKSTTESLENINKVFDEKLPWDFWTIDLRQAINYLGELTGEDLTENLLDNIFSKFCIGK

Sequences:

>Translated_464_residues
MNSFSPTEDTIAAIATAVSPGQGSIAAIRISGSSAIETSKNIVDVPGIQDWSTHKVLYGHVTEENRKKYIDEVLILVMKG
PRSFTGEDVVEIHCHGGIIPVQKILERILAFPSVRRAEPGEFSQRAVLNGRLSLTQAESISELVSARSRKAAELAINGIE
GNIQTTIQSIRKRLIEQLTEIEARIDFEEDLPLLDEKHVKNEIVAIKKDLNELIDNAKRGSWVRSGLKVALAGKPNVGKS
SLMNRLSKQEKAIVTDLPGTTRDILESEIVLEGIPVTFIDTAGLRDTKDIIEKIGISRTKKTLIHADLIILIFDYSSGWT
NEDESILKQLPVNIPLLIVGNKSDLMNDQSFEKVPKYILKKENLVILSAKTGNGEDDLINYLLKKCGSSQTHGLDIALNE
RQLDLAKSTTESLENINKVFDEKLPWDFWTIDLRQAINYLGELTGEDLTENLLDNIFSKFCIGK
>Mature_464_residues
MNSFSPTEDTIAAIATAVSPGQGSIAAIRISGSSAIETSKNIVDVPGIQDWSTHKVLYGHVTEENRKKYIDEVLILVMKG
PRSFTGEDVVEIHCHGGIIPVQKILERILAFPSVRRAEPGEFSQRAVLNGRLSLTQAESISELVSARSRKAAELAINGIE
GNIQTTIQSIRKRLIEQLTEIEARIDFEEDLPLLDEKHVKNEIVAIKKDLNELIDNAKRGSWVRSGLKVALAGKPNVGKS
SLMNRLSKQEKAIVTDLPGTTRDILESEIVLEGIPVTFIDTAGLRDTKDIIEKIGISRTKKTLIHADLIILIFDYSSGWT
NEDESILKQLPVNIPLLIVGNKSDLMNDQSFEKVPKYILKKENLVILSAKTGNGEDDLINYLLKKCGSSQTHGLDIALNE
RQLDLAKSTTESLENINKVFDEKLPWDFWTIDLRQAINYLGELTGEDLTENLLDNIFSKFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=483, Percent_Identity=33.9544513457557, Blast_Score=224, Evalue=2e-58,
Organism=Homo sapiens, GI193082988, Length=513, Percent_Identity=31.7738791423002, Blast_Score=212, Evalue=5e-55,
Organism=Homo sapiens, GI193082993, Length=473, Percent_Identity=33.4038054968288, Blast_Score=211, Evalue=8e-55,
Organism=Escherichia coli, GI2367268, Length=464, Percent_Identity=35.7758620689655, Blast_Score=271, Evalue=5e-74,
Organism=Escherichia coli, GI87082120, Length=157, Percent_Identity=35.031847133758, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17507259, Length=466, Percent_Identity=32.4034334763949, Blast_Score=198, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6323665, Length=501, Percent_Identity=31.936127744511, Blast_Score=209, Evalue=6e-55,
Organism=Drosophila melanogaster, GI45550813, Length=466, Percent_Identity=30.2575107296137, Blast_Score=195, Evalue=5e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_PROM1 (A2C018)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014093.1
- ProteinModelPortal:   A2C018
- SMR:   A2C018
- STRING:   A2C018
- GeneID:   4779880
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_02641
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   PMAR167555:NATL1_02641-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00449
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51474; Mature: 51474

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: BINDING 29-29 BINDING 91-91 BINDING 131-131 BINDING 464-464

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSFSPTEDTIAAIATAVSPGQGSIAAIRISGSSAIETSKNIVDVPGIQDWSTHKVLYGH
CCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHCCCCEEECCCCCCCCCCEEEEEE
VTEENRKKYIDEVLILVMKGPRSFTGEDVVEIHCHGGIIPVQKILERILAFPSVRRAEPG
CCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCC
EFSQRAVLNGRLSLTQAESISELVSARSRKAAELAINGIEGNIQTTIQSIRKRLIEQLTE
CCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHH
IEARIDFEEDLPLLDEKHVKNEIVAIKKDLNELIDNAKRGSWVRSGLKVALAGKPNVGKS
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCCCCCHH
SLMNRLSKQEKAIVTDLPGTTRDILESEIVLEGIPVTFIDTAGLRDTKDIIEKIGISRTK
HHHHHHHHHHCCEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCHHH
KTLIHADLIILIFDYSSGWTNEDESILKQLPVNIPLLIVGNKSDLMNDQSFEKVPKYILK
HHHHHEEEEEEEEECCCCCCCCHHHHHHHCCCCCCEEEECCCHHHCCCCHHHHHHHHHHC
KENLVILSAKTGNGEDDLINYLLKKCGSSQTHGLDIALNERQLDLAKSTTESLENINKVF
CCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
DEKLPWDFWTIDLRQAINYLGELTGEDLTENLLDNIFSKFCIGK
HHCCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNSFSPTEDTIAAIATAVSPGQGSIAAIRISGSSAIETSKNIVDVPGIQDWSTHKVLYGH
CCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHCCCCEEECCCCCCCCCCEEEEEE
VTEENRKKYIDEVLILVMKGPRSFTGEDVVEIHCHGGIIPVQKILERILAFPSVRRAEPG
CCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCC
EFSQRAVLNGRLSLTQAESISELVSARSRKAAELAINGIEGNIQTTIQSIRKRLIEQLTE
CCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHH
IEARIDFEEDLPLLDEKHVKNEIVAIKKDLNELIDNAKRGSWVRSGLKVALAGKPNVGKS
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCCCCCHH
SLMNRLSKQEKAIVTDLPGTTRDILESEIVLEGIPVTFIDTAGLRDTKDIIEKIGISRTK
HHHHHHHHHHCCEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCHHH
KTLIHADLIILIFDYSSGWTNEDESILKQLPVNIPLLIVGNKSDLMNDQSFEKVPKYILK
HHHHHEEEEEEEEECCCCCCCCHHHHHHHCCCCCCEEEECCCHHHCCCCHHHHHHHHHHC
KENLVILSAKTGNGEDDLINYLLKKCGSSQTHGLDIALNERQLDLAKSTTESLENINKVF
CCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
DEKLPWDFWTIDLRQAINYLGELTGEDLTENLLDNIFSKFCIGK
HHCCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA