Click here to switch to the map view.

The map label for this gene is spoT [H]

Identifier: 124024980

GI number: 124024980

Start: 244655

End: 246991

Strand: Reverse

Name: spoT [H]

Synonym: NATL1_02671

Alternate gene names: 124024980

Gene position: 246991-244655 (Counterclockwise)

Preceding gene: 124024982

Following gene: 124024977

Centisome position: 13.25

GC content: 38.0

Gene sequence:

>2337_bases
ATGCCAAAAGTCACCTCTGCACAAAATTCAAACCAGACAAATACGGTCTGTGATGAATTTTTTGATGGCCAGCCACGACT
TAGAACTCATCAGATTAAACATATTGATGATTATGAAATTATTTTGCCAGAATGGTTAAAAAATTGCATTGAAAATATCC
CACCGGGAATAGGCGCAAGTTGCCCAAGAGATTCTGAAGCACTTTTAGTTGCTGCTTTTGATCTTGCATATCAATTACAT
AAAGGCCAATTCAGAAAAAGTGGTGAGCCCTACATAACCCATCCAGTTGCTGTAGCTGACTTATTAAGAGAAATTGGTGC
AAGTGCTAGTGTAATAGCAGCAGGTTTTTTGCATGACGTCGTCGAAGATACAGACATCACACCAGAACAACTCGAGGGTT
ATTTTGGTAGTGAAGTTAGATATTTAGTTGAAGGTGTTACTAAATTAGGCGGGATCCATTTTAACAATCGGACAGAAGCT
CAAGCAGAGAACCTACGTAAAATGTTTCTAGCTATGTCTAGCGATATTCGAGTTGTACTAGTTAAGCTTGCAGACAGACT
TCACAATATGCGCACTATCAGTTACTTAGGAGAAGAAAAGCAAATTCGAATAGCTAGAGAAACAAGAGAAATTTATGCTC
CACTCGCTAACCGCCTAGGAATAGGACGTTTAAAATGGGAATTAGAAGACTTAGCATTTAAACTTTTAGAACCCGATCCC
TTCCGCGAGATTCAACAACAGATAGCTAGTAAGCGTAGTGAAAGGGAGGAGAGATTGAATACAACTGTTCAATTACTGAA
AGATCGCTTGAACTCCGCAGGACTTCATCAATGTGAAGTAAGTGGTAGACCAAAGCATTTATATGGAATTTGGAGCAAAA
TGCAAAGACAAAAAAAAGAGTTTCATGAAATTTTTGATGTAGCTGCTCTAAGAATTATTGTCTTAGATGTCGAGACATGT
TATCGAGCTCTTGCTGTTGTACATGATACATTCAGACCAATACCTGGACGCTTCAAGGATTACATAGGCCTTCCTAAACC
AAATGGCTATCAGTCCCTGCATACAGCTGTAATTGGCAGGCACAGACCTATTGAAGTACAAATTAGAACTCCAGAAATGC
ATAGAGTTTCAGAGTTTGGGATTGCAGCTCACTGGAAATATAAGGAAGGAGGATCTCCAGCAAATCCTGATTCAGAAAAA
TTCAATTGGTTGCGTCAATTAGTTGAATGGCAACAAGAAGATGGTGTTAATGATCATAATGACTACCTAGCATCAATCAA
AGAAGATTTATTTGACGAGGAAGTATTTGTTTTCACTCCAAAAGGAGATGTCTTGGGATTAAGGAACCGTTCAACAGCCA
TCGATTTCGCTTATCGCATTCACTCTGAAGTAGGCAATCATTGCAATGGGGCTAGAATTAATGATCGTCTATGTGTGCTG
TCAACACCTCTTGAAAATGGAGATTTTGTAGAAATTCTCACTCATAAGAGTTCTCATCCAAGCTTAGATTGGTTAAATTA
TGTTGCCACACCCACAGCTAGGAATCGCATTAGGCAATGGTATAAAAAAAGTCATCGTCAAGAAACAATATCAAGAGGGA
AGGAATTATTAGAACAAGAATTTGGTCGTAAAGGAATTGACAATTTACTCAAAGGCCATGCGATAACAAAAGTTGCGGAG
AGATGTAATCTAAAGTCTACGGAAGATTTATTAGCTGCACTTGGGTTTGGGGCCTTAACCTTACATCAAGTAATTAATAG
ATTCAGAGAAGAAATAAAAATACTAAACCAAGTACCTGAGACCGAATTAAGTGATCTCGAGCTAGCAACCCAAATTGGAA
CACAAGCAAATTTAGCTTCAAACAAATCTAAAGCTTCTCAAACAAAATATTCACCCATAGTTGGACTAGAGGGACTTGAC
TATCGACTAGGGGGGTGTTGCAGTCCTTTACCAGGAGAAGCAATACTTGGAACAGTTGCGCTTGGCAATCATGGTATCAC
CATACATCGTACTAATTGTGTCAATATAGAATCAATACCTAATAGCAGAAGACTATCAGTCCAGTGGAATACAAATGCAC
GTATCAAAGAAGAAAAGTTTCCTATTCAATTAAGGATTGAAGTTATTGACCGAGTAGGTGTACTTAAAGACATATTAATG
AGACTTTCTGATAGAGGTATTAATGTAATCGACGCAAGAGTTCAGACTTCACAGGGAAAGCCTGCATGCATAGATCTGAA
AGTTGAGCTGGAAAGTGTTAATCAATTAGAAATCACTATTAACCAAATTCGTTCAATGGTTGATGTTTTAGATATTGCTA
GAACATCATTTAATTAA

Upstream 100 bases:

>100_bases
ATTAAGTTTATTTATCTTTAAAACTTCTTTTGAGCAATTATTCATAAAAAGGTAGCAGGAAATGTTGAGTTTGAATAAAA
TAAATTAAGGTTCGGTGCTT

Downstream 100 bases:

>100_bases
TTTTTTAAATAATCCAAGATTTTAAAAAAGTGATATCTAATATATAAAAATTTACATTTTATGATAAGGTTTTTTGAGCA
AGAAATATAGACCAAGTCCT

Product: guanosine-3',5'-bis(diphosphate) 3'-diphosphatase, (ppGpp)ase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 778; Mature: 777

Protein sequence:

>778_residues
MPKVTSAQNSNQTNTVCDEFFDGQPRLRTHQIKHIDDYEIILPEWLKNCIENIPPGIGASCPRDSEALLVAAFDLAYQLH
KGQFRKSGEPYITHPVAVADLLREIGASASVIAAGFLHDVVEDTDITPEQLEGYFGSEVRYLVEGVTKLGGIHFNNRTEA
QAENLRKMFLAMSSDIRVVLVKLADRLHNMRTISYLGEEKQIRIARETREIYAPLANRLGIGRLKWELEDLAFKLLEPDP
FREIQQQIASKRSEREERLNTTVQLLKDRLNSAGLHQCEVSGRPKHLYGIWSKMQRQKKEFHEIFDVAALRIIVLDVETC
YRALAVVHDTFRPIPGRFKDYIGLPKPNGYQSLHTAVIGRHRPIEVQIRTPEMHRVSEFGIAAHWKYKEGGSPANPDSEK
FNWLRQLVEWQQEDGVNDHNDYLASIKEDLFDEEVFVFTPKGDVLGLRNRSTAIDFAYRIHSEVGNHCNGARINDRLCVL
STPLENGDFVEILTHKSSHPSLDWLNYVATPTARNRIRQWYKKSHRQETISRGKELLEQEFGRKGIDNLLKGHAITKVAE
RCNLKSTEDLLAALGFGALTLHQVINRFREEIKILNQVPETELSDLELATQIGTQANLASNKSKASQTKYSPIVGLEGLD
YRLGGCCSPLPGEAILGTVALGNHGITIHRTNCVNIESIPNSRRLSVQWNTNARIKEEKFPIQLRIEVIDRVGVLKDILM
RLSDRGINVIDARVQTSQGKPACIDLKVELESVNQLEITINQIRSMVDVLDIARTSFN

Sequences:

>Translated_778_residues
MPKVTSAQNSNQTNTVCDEFFDGQPRLRTHQIKHIDDYEIILPEWLKNCIENIPPGIGASCPRDSEALLVAAFDLAYQLH
KGQFRKSGEPYITHPVAVADLLREIGASASVIAAGFLHDVVEDTDITPEQLEGYFGSEVRYLVEGVTKLGGIHFNNRTEA
QAENLRKMFLAMSSDIRVVLVKLADRLHNMRTISYLGEEKQIRIARETREIYAPLANRLGIGRLKWELEDLAFKLLEPDP
FREIQQQIASKRSEREERLNTTVQLLKDRLNSAGLHQCEVSGRPKHLYGIWSKMQRQKKEFHEIFDVAALRIIVLDVETC
YRALAVVHDTFRPIPGRFKDYIGLPKPNGYQSLHTAVIGRHRPIEVQIRTPEMHRVSEFGIAAHWKYKEGGSPANPDSEK
FNWLRQLVEWQQEDGVNDHNDYLASIKEDLFDEEVFVFTPKGDVLGLRNRSTAIDFAYRIHSEVGNHCNGARINDRLCVL
STPLENGDFVEILTHKSSHPSLDWLNYVATPTARNRIRQWYKKSHRQETISRGKELLEQEFGRKGIDNLLKGHAITKVAE
RCNLKSTEDLLAALGFGALTLHQVINRFREEIKILNQVPETELSDLELATQIGTQANLASNKSKASQTKYSPIVGLEGLD
YRLGGCCSPLPGEAILGTVALGNHGITIHRTNCVNIESIPNSRRLSVQWNTNARIKEEKFPIQLRIEVIDRVGVLKDILM
RLSDRGINVIDARVQTSQGKPACIDLKVELESVNQLEITINQIRSMVDVLDIARTSFN
>Mature_777_residues
PKVTSAQNSNQTNTVCDEFFDGQPRLRTHQIKHIDDYEIILPEWLKNCIENIPPGIGASCPRDSEALLVAAFDLAYQLHK
GQFRKSGEPYITHPVAVADLLREIGASASVIAAGFLHDVVEDTDITPEQLEGYFGSEVRYLVEGVTKLGGIHFNNRTEAQ
AENLRKMFLAMSSDIRVVLVKLADRLHNMRTISYLGEEKQIRIARETREIYAPLANRLGIGRLKWELEDLAFKLLEPDPF
REIQQQIASKRSEREERLNTTVQLLKDRLNSAGLHQCEVSGRPKHLYGIWSKMQRQKKEFHEIFDVAALRIIVLDVETCY
RALAVVHDTFRPIPGRFKDYIGLPKPNGYQSLHTAVIGRHRPIEVQIRTPEMHRVSEFGIAAHWKYKEGGSPANPDSEKF
NWLRQLVEWQQEDGVNDHNDYLASIKEDLFDEEVFVFTPKGDVLGLRNRSTAIDFAYRIHSEVGNHCNGARINDRLCVLS
TPLENGDFVEILTHKSSHPSLDWLNYVATPTARNRIRQWYKKSHRQETISRGKELLEQEFGRKGIDNLLKGHAITKVAER
CNLKSTEDLLAALGFGALTLHQVINRFREEIKILNQVPETELSDLELATQIGTQANLASNKSKASQTKYSPIVGLEGLDY
RLGGCCSPLPGEAILGTVALGNHGITIHRTNCVNIESIPNSRRLSVQWNTNARIKEEKFPIQLRIEVIDRVGVLKDILMR
LSDRGINVIDARVQTSQGKPACIDLKVELESVNQLEITINQIRSMVDVLDIARTSFN

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=141, Percent_Identity=40.4255319148936, Blast_Score=81, Evalue=4e-15,
Organism=Escherichia coli, GI1790082, Length=722, Percent_Identity=37.9501385041551, Blast_Score=503, Evalue=1e-143,
Organism=Escherichia coli, GI1789147, Length=737, Percent_Identity=34.5997286295794, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI25143118, Length=172, Percent_Identity=32.5581395348837, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 88163; Mature: 88031

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKVTSAQNSNQTNTVCDEFFDGQPRLRTHQIKHIDDYEIILPEWLKNCIENIPPGIGAS
CCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCCC
CPRDSEALLVAAFDLAYQLHKGQFRKSGEPYITHPVAVADLLREIGASASVIAAGFLHDV
CCCCCCEEEEHHHHHHHHHHHCHHCCCCCCCEECHHHHHHHHHHHCCCHHHHHHHHHHHH
VEDTDITPEQLEGYFGSEVRYLVEGVTKLGGIHFNNRTEAQAENLRKMFLAMSSDIRVVL
HHCCCCCHHHHCCCCCHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHCCCHHHHH
VKLADRLHNMRTISYLGEEKQIRIARETREIYAPLANRLGIGRLKWELEDLAFKLLEPDP
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCH
FREIQQQIASKRSEREERLNTTVQLLKDRLNSAGLHQCEVSGRPKHLYGIWSKMQRQKKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHH
FHEIFDVAALRIIVLDVETCYRALAVVHDTFRPIPGRFKDYIGLPKPNGYQSLHTAVIGR
HHHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHCC
HRPIEVQIRTPEMHRVSEFGIAAHWKYKEGGSPANPDSEKFNWLRQLVEWQQEDGVNDHN
CCCEEEEEECCCHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHH
DYLASIKEDLFDEEVFVFTPKGDVLGLRNRSTAIDFAYRIHSEVGNHCNGARINDRLCVL
HHHHHHHHHHCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEECCEEEEE
STPLENGDFVEILTHKSSHPSLDWLNYVATPTARNRIRQWYKKSHRQETISRGKELLEQE
ECCCCCCCEEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGRKGIDNLLKGHAITKVAERCNLKSTEDLLAALGFGALTLHQVINRFREEIKILNQVPE
HCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TELSDLELATQIGTQANLASNKSKASQTKYSPIVGLEGLDYRLGGCCSPLPGEAILGTVA
CCHHHHHHHHHHCCCHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEE
LGNHGITIHRTNCVNIESIPNSRRLSVQWNTNARIKEEKFPIQLRIEVIDRVGVLKDILM
ECCCCEEEEECCEEEECCCCCCCEEEEEECCCCCEECCCCCEEEEHHHHHHHHHHHHHHH
RLSDRGINVIDARVQTSQGKPACIDLKVELESVNQLEITINQIRSMVDVLDIARTSFN
HHHCCCCEEEEEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PKVTSAQNSNQTNTVCDEFFDGQPRLRTHQIKHIDDYEIILPEWLKNCIENIPPGIGAS
CCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCCC
CPRDSEALLVAAFDLAYQLHKGQFRKSGEPYITHPVAVADLLREIGASASVIAAGFLHDV
CCCCCCEEEEHHHHHHHHHHHCHHCCCCCCCEECHHHHHHHHHHHCCCHHHHHHHHHHHH
VEDTDITPEQLEGYFGSEVRYLVEGVTKLGGIHFNNRTEAQAENLRKMFLAMSSDIRVVL
HHCCCCCHHHHCCCCCHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHCCCHHHHH
VKLADRLHNMRTISYLGEEKQIRIARETREIYAPLANRLGIGRLKWELEDLAFKLLEPDP
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCH
FREIQQQIASKRSEREERLNTTVQLLKDRLNSAGLHQCEVSGRPKHLYGIWSKMQRQKKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHH
FHEIFDVAALRIIVLDVETCYRALAVVHDTFRPIPGRFKDYIGLPKPNGYQSLHTAVIGR
HHHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHCC
HRPIEVQIRTPEMHRVSEFGIAAHWKYKEGGSPANPDSEKFNWLRQLVEWQQEDGVNDHN
CCCEEEEEECCCHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHH
DYLASIKEDLFDEEVFVFTPKGDVLGLRNRSTAIDFAYRIHSEVGNHCNGARINDRLCVL
HHHHHHHHHHCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEECCEEEEE
STPLENGDFVEILTHKSSHPSLDWLNYVATPTARNRIRQWYKKSHRQETISRGKELLEQE
ECCCCCCCEEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGRKGIDNLLKGHAITKVAERCNLKSTEDLLAALGFGALTLHQVINRFREEIKILNQVPE
HCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TELSDLELATQIGTQANLASNKSKASQTKYSPIVGLEGLDYRLGGCCSPLPGEAILGTVA
CCHHHHHHHHHHCCCHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEE
LGNHGITIHRTNCVNIESIPNSRRLSVQWNTNARIKEEKFPIQLRIEVIDRVGVLKDILM
ECCCCEEEEECCEEEECCCCCCCEEEEEECCCCCEECCCCCEEEEHHHHHHHHHHHHHHH
RLSDRGINVIDARVQTSQGKPACIDLKVELESVNQLEITINQIRSMVDVLDIARTSFN
HHHCCCCEEEEEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]