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The map label for this gene is ndhF [H]

Identifier: 124024934

GI number: 124024934

Start: 205104

End: 207110

Strand: Direct

Name: ndhF [H]

Synonym: NATL1_02211

Alternate gene names: 124024934

Gene position: 205104-207110 (Clockwise)

Preceding gene: 124024933

Following gene: 124024935

Centisome position: 11.0

GC content: 40.41

Gene sequence:

>2007_bases
ATGCATTCGGCTGCAGATTTTGCTTGGTTAATCCCACTTCTTCCACTTTGTGGGGCAGTTTTAATTGGTTTGGGATTAAT
TAGTTTTAACGATCTTTTTAATCGTTCAAGGAAACCCGTGGCAATAACCTTGTTGACTTCCGTAGGTGCTTCGGCATTTA
TTAGCTATGCAGTTTTAGCCGAGCAACTCTCAGGTAAACCTCCAGTTGAGCACTTGTTTATTTGGGCTAGTGCAGGCTCA
TTTGAGTTGCCAATGGGTTATGTGATTGATCCTCTTGCGGCAGTCATGCTCGCTCTTGTAACTACAATCGCTTTCTTGGT
AATGATTTATTCCCATGGTTATATGGCACATGATCCTGGGTATGTTCGTTTTTTTACTTATCTAGCCTTATTCAGCAGCT
CAATGCTTGGTCTAATTGTTAGTCCAAATTTGCTTGAAATATATGTTTTTTGGGAATTGGTTGGTATGTGTTCATATTTA
TTAGTTGGCTTTTGGTATGACAGAGACGGTGCTGCACATGCAGCTCAAAAGGCTTTTATCGTAAATAGAGTGGGTGACTT
TGGCTTATTGCTTGGAATTTTGGGTCTTTTTTGGGCGACAGGTAGCTTTGATTTTCATGGCATAGCTGATGGTTTATCAG
TTGCAGTTGGTTCTGGGAATGTACCAATGTGGGCGGCTTTGACTCTTTGCATACTTGTCTTTATGGGGCCAATGGCTAAG
TCTGCACAATTTCCATTGCATGTTTGGTTGCCCGATGCAATGGAAGGTCCAACTCCCATATCTGCTCTTATCCATGCAGC
AACAATGGTTGCAGCAGGAGTTTTTTTAGTAGCAAGGCTTGATCCTTTGTTTAGCCAGTTCCCTTTCGTTGGATTATTTA
TCGCACTTATTGGAACTATCACTTGTTTCCTAGGTGCATCTATCGCTTTGACTCAAATGGACTTGAAAAAAGGGTTGGCT
TACAGCACAGTTTCTCAACTTGGATACATGATGCTTGCGATGGGATGTGGTGCACCAGTTGCAGGAATGTTCCACCTTGT
TACTCATGCTTGTTTTAAGGCCATGTTATTCCTTGGTTCAGGTTCAGTGATTCATGCCATGGAGGAGGTCGTTGGTCATG
AACCAATTCTTGCTCAAGACATGCGATTGATGGGAGGTCTACGTAAAAAAATGCCTATTACAGCAATAACTTTTTTCATT
GGCTGCATTGCTATTAGTGGCATACCTCCTCTCGCAGGTTTTTGGAGTAAAGATGAAATTCTAGGTCAAGCTTTTAACAG
TTTTCCAATTCTTTGGTTTATTGGTTTTTTAACGGCAGGAATGACAGCTTTCTACATGTTTAGGCTTTATTTTCTAACTT
TTGAGGGCGACTTTCGCGGCGAAAATAAAGAGATGCAGCTTTCTTTGCTTTCTTTAGCAGGCAAAGACAAAGATGAAGAG
CATGAAGATCATGAAATTGGCAAAATACATGAATCTGCGTGGCCCATGACATTTCCCTTGGCAGTTTTGGCAATTCCTTC
TGTCCTGATTGGATTTATAGGTGTTCCTTGGAACAGCATTTTTGCAAACTTGTTAGATCCTGTTGAGGCTATTGAAGCTG
CAGAAAAGTTTAGTTGGGGAGAATTTCTTCCACTTGCAACTGCCTCAGTAGCAATCTCATCCGCAGGGATATTATTGGCT
GTCTTGACTTATTATTTAAAAAGATTTGATCTTGGTGTTTCTCTATCCAAGAAGTACCCTCAAATCAATTCATTTCTTCA
GAACAAATGGTATTTAGATGATATAAATGAGAAGATTTTTGTAAAAGGTAGTAGAAAACTCGCAAGAGAAGTACTAGAAG
TCGACGCAAAAGTAGTTGATGGAGTTGTCAATCTGACTGGATTATTGACTCTTGGTAGTGGAGAAGGGCTTAAATATTTT
GAGACAGGAAGAGCTCAGTTTTATGCATTGATTGTTTTTGGAGGAGTAATTGCTCTGGTTGCACTTTTTGGAGTTGTTGG
TGCATGA

Upstream 100 bases:

>100_bases
CATAAATTTATTTCCGTTGGAGCTCAGAGATTTCTCTCTTTTGATTTAACTGAATTACTAGCAAGAATTGCCTAAACTTA
TCTCAATATGATTTAGCTGG

Downstream 100 bases:

>100_bases
TTTTTACATAATTTTTTTAGGTGTGTGTTTCATCCCCTATTTAGAGGGTGCCAATAATTTCATAATTTCTAAACTCAAAA
TGTGTAATTAATTTCGAGTC

Product: NAD(P)H-quinone oxidoreductase subunit F

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MHSAADFAWLIPLLPLCGAVLIGLGLISFNDLFNRSRKPVAITLLTSVGASAFISYAVLAEQLSGKPPVEHLFIWASAGS
FELPMGYVIDPLAAVMLALVTTIAFLVMIYSHGYMAHDPGYVRFFTYLALFSSSMLGLIVSPNLLEIYVFWELVGMCSYL
LVGFWYDRDGAAHAAQKAFIVNRVGDFGLLLGILGLFWATGSFDFHGIADGLSVAVGSGNVPMWAALTLCILVFMGPMAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLVARLDPLFSQFPFVGLFIALIGTITCFLGASIALTQMDLKKGLA
YSTVSQLGYMMLAMGCGAPVAGMFHLVTHACFKAMLFLGSGSVIHAMEEVVGHEPILAQDMRLMGGLRKKMPITAITFFI
GCIAISGIPPLAGFWSKDEILGQAFNSFPILWFIGFLTAGMTAFYMFRLYFLTFEGDFRGENKEMQLSLLSLAGKDKDEE
HEDHEIGKIHESAWPMTFPLAVLAIPSVLIGFIGVPWNSIFANLLDPVEAIEAAEKFSWGEFLPLATASVAISSAGILLA
VLTYYLKRFDLGVSLSKKYPQINSFLQNKWYLDDINEKIFVKGSRKLAREVLEVDAKVVDGVVNLTGLLTLGSGEGLKYF
ETGRAQFYALIVFGGVIALVALFGVVGA

Sequences:

>Translated_668_residues
MHSAADFAWLIPLLPLCGAVLIGLGLISFNDLFNRSRKPVAITLLTSVGASAFISYAVLAEQLSGKPPVEHLFIWASAGS
FELPMGYVIDPLAAVMLALVTTIAFLVMIYSHGYMAHDPGYVRFFTYLALFSSSMLGLIVSPNLLEIYVFWELVGMCSYL
LVGFWYDRDGAAHAAQKAFIVNRVGDFGLLLGILGLFWATGSFDFHGIADGLSVAVGSGNVPMWAALTLCILVFMGPMAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLVARLDPLFSQFPFVGLFIALIGTITCFLGASIALTQMDLKKGLA
YSTVSQLGYMMLAMGCGAPVAGMFHLVTHACFKAMLFLGSGSVIHAMEEVVGHEPILAQDMRLMGGLRKKMPITAITFFI
GCIAISGIPPLAGFWSKDEILGQAFNSFPILWFIGFLTAGMTAFYMFRLYFLTFEGDFRGENKEMQLSLLSLAGKDKDEE
HEDHEIGKIHESAWPMTFPLAVLAIPSVLIGFIGVPWNSIFANLLDPVEAIEAAEKFSWGEFLPLATASVAISSAGILLA
VLTYYLKRFDLGVSLSKKYPQINSFLQNKWYLDDINEKIFVKGSRKLAREVLEVDAKVVDGVVNLTGLLTLGSGEGLKYF
ETGRAQFYALIVFGGVIALVALFGVVGA
>Mature_668_residues
MHSAADFAWLIPLLPLCGAVLIGLGLISFNDLFNRSRKPVAITLLTSVGASAFISYAVLAEQLSGKPPVEHLFIWASAGS
FELPMGYVIDPLAAVMLALVTTIAFLVMIYSHGYMAHDPGYVRFFTYLALFSSSMLGLIVSPNLLEIYVFWELVGMCSYL
LVGFWYDRDGAAHAAQKAFIVNRVGDFGLLLGILGLFWATGSFDFHGIADGLSVAVGSGNVPMWAALTLCILVFMGPMAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLVARLDPLFSQFPFVGLFIALIGTITCFLGASIALTQMDLKKGLA
YSTVSQLGYMMLAMGCGAPVAGMFHLVTHACFKAMLFLGSGSVIHAMEEVVGHEPILAQDMRLMGGLRKKMPITAITFFI
GCIAISGIPPLAGFWSKDEILGQAFNSFPILWFIGFLTAGMTAFYMFRLYFLTFEGDFRGENKEMQLSLLSLAGKDKDEE
HEDHEIGKIHESAWPMTFPLAVLAIPSVLIGFIGVPWNSIFANLLDPVEAIEAAEKFSWGEFLPLATASVAISSAGILLA
VLTYYLKRFDLGVSLSKKYPQINSFLQNKWYLDDINEKIFVKGSRKLAREVLEVDAKVVDGVVNLTGLLTLGSGEGLKYF
ETGRAQFYALIVFGGVIALVALFGVVGA

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=388, Percent_Identity=39.1752577319588, Blast_Score=265, Evalue=2e-70,
Organism=Escherichia coli, GI1788614, Length=654, Percent_Identity=37.3088685015291, Blast_Score=335, Evalue=7e-93,
Organism=Escherichia coli, GI1788829, Length=365, Percent_Identity=32.8767123287671, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1788827, Length=408, Percent_Identity=29.656862745098, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1788831, Length=353, Percent_Identity=31.1614730878187, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI1788613, Length=428, Percent_Identity=25.4672897196262, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI2367154, Length=403, Percent_Identity=24.5657568238213, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI145693160, Length=293, Percent_Identity=25.2559726962457, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 72400; Mature: 72400

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSAADFAWLIPLLPLCGAVLIGLGLISFNDLFNRSRKPVAITLLTSVGASAFISYAVLA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCHHHHHHHHHHH
EQLSGKPPVEHLFIWASAGSFELPMGYVIDPLAAVMLALVTTIAFLVMIYSHGYMAHDPG
HHHCCCCCHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YVRFFTYLALFSSSMLGLIVSPNLLEIYVFWELVGMCSYLLVGFWYDRDGAAHAAQKAFI
HHHHHHHHHHHHCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHH
VNRVGDFGLLLGILGLFWATGSFDFHGIADGLSVAVGSGNVPMWAALTLCILVFMGPMAK
HHHCCHHHHHHHHHHHHHHCCCCCCCCHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCC
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLVARLDPLFSQFPFVGLFIALIGTI
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TCFLGASIALTQMDLKKGLAYSTVSQLGYMMLAMGCGAPVAGMFHLVTHACFKAMLFLGS
HHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
GSVIHAMEEVVGHEPILAQDMRLMGGLRKKMPITAITFFIGCIAISGIPPLAGFWSKDEI
CHHHHHHHHHHCCCCHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHH
LGQAFNSFPILWFIGFLTAGMTAFYMFRLYFLTFEGDFRGENKEMQLSLLSLAGKDKDEE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHCCCCCCCC
HEDHEIGKIHESAWPMTFPLAVLAIPSVLIGFIGVPWNSIFANLLDPVEAIEAAEKFSWG
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
EFLPLATASVAISSAGILLAVLTYYLKRFDLGVSLSKKYPQINSFLQNKWYLDDINEKIF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEE
VKGSRKLAREVLEVDAKVVDGVVNLTGLLTLGSGEGLKYFETGRAQFYALIVFGGVIALV
EECCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHCCCCHHHHHHHHHHHHHHHH
ALFGVVGA
HHHHHHCC
>Mature Secondary Structure
MHSAADFAWLIPLLPLCGAVLIGLGLISFNDLFNRSRKPVAITLLTSVGASAFISYAVLA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCHHHHHHHHHHH
EQLSGKPPVEHLFIWASAGSFELPMGYVIDPLAAVMLALVTTIAFLVMIYSHGYMAHDPG
HHHCCCCCHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YVRFFTYLALFSSSMLGLIVSPNLLEIYVFWELVGMCSYLLVGFWYDRDGAAHAAQKAFI
HHHHHHHHHHHHCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHH
VNRVGDFGLLLGILGLFWATGSFDFHGIADGLSVAVGSGNVPMWAALTLCILVFMGPMAK
HHHCCHHHHHHHHHHHHHHCCCCCCCCHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCC
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLVARLDPLFSQFPFVGLFIALIGTI
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TCFLGASIALTQMDLKKGLAYSTVSQLGYMMLAMGCGAPVAGMFHLVTHACFKAMLFLGS
HHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
GSVIHAMEEVVGHEPILAQDMRLMGGLRKKMPITAITFFIGCIAISGIPPLAGFWSKDEI
CHHHHHHHHHHCCCCHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHH
LGQAFNSFPILWFIGFLTAGMTAFYMFRLYFLTFEGDFRGENKEMQLSLLSLAGKDKDEE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHCCCCCCCC
HEDHEIGKIHESAWPMTFPLAVLAIPSVLIGFIGVPWNSIFANLLDPVEAIEAAEKFSWG
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
EFLPLATASVAISSAGILLAVLTYYLKRFDLGVSLSKKYPQINSFLQNKWYLDDINEKIF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEE
VKGSRKLAREVLEVDAKVVDGVVNLTGLLTLGSGEGLKYFETGRAQFYALIVFGGVIALV
EECCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHCCCCHHHHHHHHHHHHHHHH
ALFGVVGA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]