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The map label for this gene is gatB

Identifier: 124024775

GI number: 124024775

Start: 68204

End: 69679

Strand: Direct

Name: gatB

Synonym: NATL1_00621

Alternate gene names: 124024775

Gene position: 68204-69679 (Clockwise)

Preceding gene: 124024773

Following gene: 124024777

Centisome position: 3.66

GC content: 35.84

Gene sequence:

>1476_bases
ATGTCAGAATCAAATGTTTCTTGGGAAGTTGTAATTGGATTAGAGACGCATGTGCAGTTGGGGACTAAGAGCAAAATATT
TACTAGTGCATCAACAACCTTTGGTGACGATCCAAATACTCATATTGACCCAGTGGTTTGCGGATTGCCAGGCACCTTGC
CAGTCCTAAATAAAAAGGTTTTGGAATATGCAGTAAAAGCAGCGATGGCTTTGAATCTAAATATTGCCTCTCACAGTAAA
TTTGATAGAAAGCAATATTTTTATCCCGACTTACCAAAAAATTATCAAATCTCTCAGTTTGATGAACCTATTGCAGAAGA
TGGTTGGATAGAAGTAGAAGTAGCTGAAAAAGGAAAAGAAACTTATGTCAAAAAAATAGGTATTGAGAGACTACATATGG
AGGAGGATGCAGGAAAACTGGTGCACGCAGGTAGTGATCAATTGTCAGGTTCTACTCATTCTTTGGTTGATTACAACAGA
GCAGGCGTAGCACTTGCAGAAATAGTAAGCAAACCTGATTTAAGAACAGGTAGAGAGGCTGCTGAGTATGCAGCTGAAGT
TAGAAGAATCATGCGTTATTTGGGAGTATCTGATGGGAATATGCAGGAAGGTTCCTTGCGATGTGATGTGAATATTTCAG
TCAGACCAACTATCAATGACCCATTTGGCACAAAAGTAGAAATAAAAAATATGAATTCATTTTCAGCTATTCAGAAAGCT
TGTGAATATGAAATTAAGCGGCAAATTAAAGCTTATGAATCTGGAGAAGAAGTAAAACAAGAAACAAGGTTATGGGATGA
AGGTAAGCAACTGACTAAAAGTATGAGATCCAAAGAAGGCAGTAGCGATTATCGTTATTTTCCCGATCCTGATCTAGGTC
CGATAGAAGTAAGTAATGAGTTAAAAGAAAAATGGCGTTCAGAATTACCAGAATTGCCAGCTGCAAAAAGAAATAGATAT
TCAACAGAACTTGGCCTTTCTATTTATGATGCAAGAGTTTTGACTGATGAATCTTCAATGGCGACATATTTTGAAAAAGT
TGTTAACGAAGGTGGAGCAGCTAAATCTTCAGCAAATTGGATAACTGGAGATCTAGCAGCCTATATAAACTCTAATAGAT
TAACATTTGATCAATTACATTTTCAGCCGAGTGAATTAGCAGAAATGTTAAAAATGATTGATACTGGAGAAATAAGCGGC
AAAATTGCAAAGGAAATATTACCTGAACTATTAAGTAAAGGTGGTTCTCCAAAGCAATTAGTTCAAGAACGTGGTTTAGG
TATGATTGGTGATCCTAAAGTTATTGAAGAAATTATCGATCAATTGATTCTTAAGCATCCAAATGAGGTTGAATCTTTTA
GATCTGGGAAGAAGAAATTGTTAGGGTTTTTTGTTGGTCAATTAATGAAGGAAACAAAAGGGAAAGCAGATCCAAAACTC
GCTAATCAAATATTAAATAAAAAATTACAAGGTTAG

Upstream 100 bases:

>100_bases
TTGCTTGGATTTGGAAAATGTTATTTTCAATGTTTTAGATTACGCTAGCTTCCATGACTGAGCTTTTCTTTCATAAGATT
CATATATCTAGGTCAAATTA

Downstream 100 bases:

>100_bases
AATTTATATAATTTTTTTAATTGTAATTTCCATGTCTCATCATCATTAGAGTTATTAATGATGTGATCTGCAAATTGTTT
TTTCAAAGAGTTTGCCCATT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSESNVSWEVVIGLETHVQLGTKSKIFTSASTTFGDDPNTHIDPVVCGLPGTLPVLNKKVLEYAVKAAMALNLNIASHSK
FDRKQYFYPDLPKNYQISQFDEPIAEDGWIEVEVAEKGKETYVKKIGIERLHMEEDAGKLVHAGSDQLSGSTHSLVDYNR
AGVALAEIVSKPDLRTGREAAEYAAEVRRIMRYLGVSDGNMQEGSLRCDVNISVRPTINDPFGTKVEIKNMNSFSAIQKA
CEYEIKRQIKAYESGEEVKQETRLWDEGKQLTKSMRSKEGSSDYRYFPDPDLGPIEVSNELKEKWRSELPELPAAKRNRY
STELGLSIYDARVLTDESSMATYFEKVVNEGGAAKSSANWITGDLAAYINSNRLTFDQLHFQPSELAEMLKMIDTGEISG
KIAKEILPELLSKGGSPKQLVQERGLGMIGDPKVIEEIIDQLILKHPNEVESFRSGKKKLLGFFVGQLMKETKGKADPKL
ANQILNKKLQG

Sequences:

>Translated_491_residues
MSESNVSWEVVIGLETHVQLGTKSKIFTSASTTFGDDPNTHIDPVVCGLPGTLPVLNKKVLEYAVKAAMALNLNIASHSK
FDRKQYFYPDLPKNYQISQFDEPIAEDGWIEVEVAEKGKETYVKKIGIERLHMEEDAGKLVHAGSDQLSGSTHSLVDYNR
AGVALAEIVSKPDLRTGREAAEYAAEVRRIMRYLGVSDGNMQEGSLRCDVNISVRPTINDPFGTKVEIKNMNSFSAIQKA
CEYEIKRQIKAYESGEEVKQETRLWDEGKQLTKSMRSKEGSSDYRYFPDPDLGPIEVSNELKEKWRSELPELPAAKRNRY
STELGLSIYDARVLTDESSMATYFEKVVNEGGAAKSSANWITGDLAAYINSNRLTFDQLHFQPSELAEMLKMIDTGEISG
KIAKEILPELLSKGGSPKQLVQERGLGMIGDPKVIEEIIDQLILKHPNEVESFRSGKKKLLGFFVGQLMKETKGKADPKL
ANQILNKKLQG
>Mature_490_residues
SESNVSWEVVIGLETHVQLGTKSKIFTSASTTFGDDPNTHIDPVVCGLPGTLPVLNKKVLEYAVKAAMALNLNIASHSKF
DRKQYFYPDLPKNYQISQFDEPIAEDGWIEVEVAEKGKETYVKKIGIERLHMEEDAGKLVHAGSDQLSGSTHSLVDYNRA
GVALAEIVSKPDLRTGREAAEYAAEVRRIMRYLGVSDGNMQEGSLRCDVNISVRPTINDPFGTKVEIKNMNSFSAIQKAC
EYEIKRQIKAYESGEEVKQETRLWDEGKQLTKSMRSKEGSSDYRYFPDPDLGPIEVSNELKEKWRSELPELPAAKRNRYS
TELGLSIYDARVLTDESSMATYFEKVVNEGGAAKSSANWITGDLAAYINSNRLTFDQLHFQPSELAEMLKMIDTGEISGK
IAKEILPELLSKGGSPKQLVQERGLGMIGDPKVIEEIIDQLILKHPNEVESFRSGKKKLLGFFVGQLMKETKGKADPKLA
NQILNKKLQG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=499, Percent_Identity=36.2725450901804, Blast_Score=310, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI115534094, Length=463, Percent_Identity=31.317494600432, Blast_Score=213, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=508, Percent_Identity=30.511811023622, Blast_Score=227, Evalue=4e-60,
Organism=Drosophila melanogaster, GI45550808, Length=499, Percent_Identity=35.0701402805611, Blast_Score=284, Evalue=8e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_PROM1 (A2BZG6)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001013891.1
- ProteinModelPortal:   A2BZG6
- STRING:   A2BZG6
- GeneID:   4779171
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_00621
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   PMAR167555:NATL1_00621-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54761; Mature: 54630

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESNVSWEVVIGLETHVQLGTKSKIFTSASTTFGDDPNTHIDPVVCGLPGTLPVLNKKV
CCCCCCCEEEEEEEECCEECCCCCCEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHHH
LEYAVKAAMALNLNIASHSKFDRKQYFYPDLPKNYQISQFDEPIAEDGWIEVEVAEKGKE
HHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHCCHH
TYVKKIGIERLHMEEDAGKLVHAGSDQLSGSTHSLVDYNRAGVALAEIVSKPDLRTGREA
HHHHHHCHHHHHCHHHCCCEEECCCCCCCCCCHHHHCCCCCHHHHHHHHCCCCCCCCHHH
AEYAAEVRRIMRYLGVSDGNMQEGSLRCDVNISVRPTINDPFGTKVEIKNMNSFSAIQKA
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHH
CEYEIKRQIKAYESGEEVKQETRLWDEGKQLTKSMRSKEGSSDYRYFPDPDLGPIEVSNE
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
LKEKWRSELPELPAAKRNRYSTELGLSIYDARVLTDESSMATYFEKVVNEGGAAKSSANW
HHHHHHHHCCCCCCHHHCCCCHHCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCC
ITGDLAAYINSNRLTFDQLHFQPSELAEMLKMIDTGEISGKIAKEILPELLSKGGSPKQL
HHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHH
VQERGLGMIGDPKVIEEIIDQLILKHPNEVESFRSGKKKLLGFFVGQLMKETKGKADPKL
HHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ANQILNKKLQG
HHHHHHHHCCC
>Mature Secondary Structure 
SESNVSWEVVIGLETHVQLGTKSKIFTSASTTFGDDPNTHIDPVVCGLPGTLPVLNKKV
CCCCCCEEEEEEEECCEECCCCCCEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHHH
LEYAVKAAMALNLNIASHSKFDRKQYFYPDLPKNYQISQFDEPIAEDGWIEVEVAEKGKE
HHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHCCHH
TYVKKIGIERLHMEEDAGKLVHAGSDQLSGSTHSLVDYNRAGVALAEIVSKPDLRTGREA
HHHHHHCHHHHHCHHHCCCEEECCCCCCCCCCHHHHCCCCCHHHHHHHHCCCCCCCCHHH
AEYAAEVRRIMRYLGVSDGNMQEGSLRCDVNISVRPTINDPFGTKVEIKNMNSFSAIQKA
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHH
CEYEIKRQIKAYESGEEVKQETRLWDEGKQLTKSMRSKEGSSDYRYFPDPDLGPIEVSNE
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
LKEKWRSELPELPAAKRNRYSTELGLSIYDARVLTDESSMATYFEKVVNEGGAAKSSANW
HHHHHHHHCCCCCCHHHCCCCHHCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCC
ITGDLAAYINSNRLTFDQLHFQPSELAEMLKMIDTGEISGKIAKEILPELLSKGGSPKQL
HHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHH
VQERGLGMIGDPKVIEEIIDQLILKHPNEVESFRSGKKKLLGFFVGQLMKETKGKADPKL
HHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ANQILNKKLQG
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA