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The map label for this gene is ybiK [C]

Identifier: 124027291

GI number: 124027291

Start: 384655

End: 385611

Strand: Direct

Name: ybiK [C]

Synonym: Hbut_0399

Alternate gene names: 124027291

Gene position: 384655-385611 (Clockwise)

Preceding gene: 124027290

Following gene: 124027292

Centisome position: 23.07

GC content: 58.1

Gene sequence:

>957_bases
GTGGCTATAACCGTCTATGGCCCTGTAAGGCTTGGCGACCCCGCCATTATAACTCATGGCGGTGCTGGTAGCTGGAAGGC
CCTTGAAAGGATAGACCCGGCAGTAGAGGCTACAAGATCAGCAGCCATGGCTGGCCTTAGAGCTGCTGCTAAGGGCGTCC
TAGAAGCTCTTGTAGAGGCCATCGCATCGCTAGAGGATAGTGGTGTGCTTAATGCCGGGCTTGGAAGCGTATTAACCTAC
GACGGCCGCGTAGAGATGGATGCTGGGCTCATGCTGGACGATATGCGGGCCGGCGCAGTGGCTGTAGTATCATACCCTCG
TAATCCTATAAGACTCGCTGCATACGTGGCGGAAAACCTTGACCACATAATAATTGCCGGTCCCGCCGCTGATAGGCTAG
CAGAGAGGCTAGGTCTTGAGAAGCATCCAGGCCCGAGCCAGGCTGCCCTGGAGAGGTGGAGAAAGCTTAGGGAGCAGCTA
GAACGAGGCGAGGGCCCTAGGTGGGCCCGGAAAATAGCCGAACTCTACGGCGATACTGTGGGCGCGGTAGTTCTCGTCTC
GGGCCGACTCGCAGCCGGCACAAGCACCGGGGGTATCGCGTTAAAGCATCCAGGCCGCGTTGGTGACTCCCCCATTCCCG
GTGCGGGGTTCTACGTTGAGCACGGTGTTGGTGGCTGTGCTGCAACCGGGATAGGCGAGACGATAATACTCAGCAGACCT
TGCATACACGCCGTCAAGCTGCTAGCTGAGGGCATGCCCGTAGAGGAGGCTGCAAGGGCAGCTGTTGCCAGACACACAAG
ACTATTCGGCAGCGATAACCTCGGCATAATACTGCTAGACGCCAAGGGCAATGCCGCTGCCGCAATAAACACTGAGGGCA
TGCCCGTAGGCCTCGCAGGGGCTGAGCTAGCAGAACCTCTAGGAGTGATGCTCCGAAGAGGAGAGGAGAGAGCATAA

Upstream 100 bases:

>100_bases
CATGGTATAGCCTAGACTACCGGAGTAGAGCACGCCAGCTTGCCGAAGAAGCCAAAAAGACCGCTATGAATAACCGCGAA
GCTGCACGGAGGGATAGGCC

Downstream 100 bases:

>100_bases
AGATAAAGAAGCCCCTGTGTCTAGCCAGCGGCCGGGTAGGGAAGGAAGAGCTGAGTGGAGGCTGGCAGGCATGGAGTACA
TATATGCTAGCCTCGTGCTC

Product: L asparaginase

Products: NA

Alternate protein names: L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MAITVYGPVRLGDPAIITHGGAGSWKALERIDPAVEATRSAAMAGLRAAAKGVLEALVEAIASLEDSGVLNAGLGSVLTY
DGRVEMDAGLMLDDMRAGAVAVVSYPRNPIRLAAYVAENLDHIIIAGPAADRLAERLGLEKHPGPSQAALERWRKLREQL
ERGEGPRWARKIAELYGDTVGAVVLVSGRLAAGTSTGGIALKHPGRVGDSPIPGAGFYVEHGVGGCAATGIGETIILSRP
CIHAVKLLAEGMPVEEAARAAVARHTRLFGSDNLGIILLDAKGNAAAAINTEGMPVGLAGAELAEPLGVMLRRGEERA

Sequences:

>Translated_318_residues
MAITVYGPVRLGDPAIITHGGAGSWKALERIDPAVEATRSAAMAGLRAAAKGVLEALVEAIASLEDSGVLNAGLGSVLTY
DGRVEMDAGLMLDDMRAGAVAVVSYPRNPIRLAAYVAENLDHIIIAGPAADRLAERLGLEKHPGPSQAALERWRKLREQL
ERGEGPRWARKIAELYGDTVGAVVLVSGRLAAGTSTGGIALKHPGRVGDSPIPGAGFYVEHGVGGCAATGIGETIILSRP
CIHAVKLLAEGMPVEEAARAAVARHTRLFGSDNLGIILLDAKGNAAAAINTEGMPVGLAGAELAEPLGVMLRRGEERA
>Mature_317_residues
AITVYGPVRLGDPAIITHGGAGSWKALERIDPAVEATRSAAMAGLRAAAKGVLEALVEAIASLEDSGVLNAGLGSVLTYD
GRVEMDAGLMLDDMRAGAVAVVSYPRNPIRLAAYVAENLDHIIIAGPAADRLAERLGLEKHPGPSQAALERWRKLREQLE
RGEGPRWARKIAELYGDTVGAVVLVSGRLAAGTSTGGIALKHPGRVGDSPIPGAGFYVEHGVGGCAATGIGETIILSRPC
IHAVKLLAEGMPVEEAARAAVARHTRLFGSDNLGIILLDAKGNAAAAINTEGMPVGLAGAELAEPLGVMLRRGEERA

Specific function: Unknown

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=289, Percent_Identity=34.9480968858132, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI145275200, Length=289, Percent_Identity=34.9480968858132, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI98991769, Length=269, Percent_Identity=30.1115241635688, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI285002251, Length=288, Percent_Identity=29.5138888888889, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1787050, Length=300, Percent_Identity=34, Blast_Score=120, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17569239, Length=327, Percent_Identity=27.5229357798165, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI18921183, Length=309, Percent_Identity=32.0388349514563, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24652135, Length=310, Percent_Identity=28.0645161290323, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI221330102, Length=310, Percent_Identity=28.0645161290323, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24655553, Length=245, Percent_Identity=27.7551020408163, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24657358, Length=284, Percent_Identity=25, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 32854; Mature: 32723

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITVYGPVRLGDPAIITHGGAGSWKALERIDPAVEATRSAAMAGLRAAAKGVLEALVEA
CEEEEEECEEECCCEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IASLEDSGVLNAGLGSVLTYDGRVEMDAGLMLDDMRAGAVAVVSYPRNPIRLAAYVAENL
HHCCCCCCCEECCCCCEEEECCEEEECCCCEEECCCCCEEEEEECCCCHHHHHHHHHCCC
DHIIIAGPAADRLAERLGLEKHPGPSQAALERWRKLREQLERGEGPRWARKIAELYGDTV
CEEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
GAVVLVSGRLAAGTSTGGIALKHPGRVGDSPIPGAGFYVEHGVGGCAATGIGETIILSRP
CEEEEEECCEECCCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCHHHCCCCCEEEECCH
CIHAVKLLAEGMPVEEAARAAVARHTRLFGSDNLGIILLDAKGNAAAAINTEGMPVGLAG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCEECCC
AELAEPLGVMLRRGEERA
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AITVYGPVRLGDPAIITHGGAGSWKALERIDPAVEATRSAAMAGLRAAAKGVLEALVEA
EEEEEECEEECCCEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IASLEDSGVLNAGLGSVLTYDGRVEMDAGLMLDDMRAGAVAVVSYPRNPIRLAAYVAENL
HHCCCCCCCEECCCCCEEEECCEEEECCCCEEECCCCCEEEEEECCCCHHHHHHHHHCCC
DHIIIAGPAADRLAERLGLEKHPGPSQAALERWRKLREQLERGEGPRWARKIAELYGDTV
CEEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
GAVVLVSGRLAAGTSTGGIALKHPGRVGDSPIPGAGFYVEHGVGGCAATGIGETIILSRP
CEEEEEECCEECCCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCHHHCCCCCEEEECCH
CIHAVKLLAEGMPVEEAARAAVARHTRLFGSDNLGIILLDAKGNAAAAINTEGMPVGLAG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCEECCC
AELAEPLGVMLRRGEERA
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]