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The map label for this gene is carA [H]

Identifier: 124027200

GI number: 124027200

Start: 290749

End: 291945

Strand: Reverse

Name: carA [H]

Synonym: Hbut_0303

Alternate gene names: 124027200

Gene position: 291945-290749 (Counterclockwise)

Preceding gene: 124027201

Following gene: 124027199

Centisome position: 17.51

GC content: 58.15

Gene sequence:

>1197_bases
GTGCAAATAGGCGATATAAAAGTCTATAGAATGCCCCTCCTTAGGTGTAAGACGAGTCTGCGTGCAAGGCTGCTACTAGC
CGATGGTGTTGCTGTTGAGGGCTGCGGCTTTGGCGCAGAGGCAACCCGTGTCGGCGAAGTTGTATTTACGACGGCCATGA
CCGGGTACCCTGAGAGCCTCACCGACCCAAGCTACCGTGGTCAGATACTCGTGGAGACACACCCGATGATAGGCAACTAT
GGTGTTCCGAGTAGAGACAGGCAACTCCACGGCGTCCCAGTCGACTACGAGTCGGACCACATACAGGTGGAAGGGTTCGT
GGTAGCAGAACTGCCGCAGCCTAGCCACTACGCATCCGTAATGAGTCTCCACGAGTGGCTGAGGAGCGAGGGCGTACCAG
GAATATACCGTGTCGATACAAGGTTTCTTGTGAAGCGGATACGCGAGTATGGCGTTGTAATGGGCATAATATCGGTTTAC
AGGGATGGCGAGGAGCCCCCGGGCTGGGATGAGCTTGCACGGAAGCTGGTATCATCGAAATCCTACGACGAGAGGATATT
TGCTCTCGAGGTCAGCCCTAGGAAGCCCATAGTACATAGGCCCAGGGGTAGGGTTAGAGCACGTGTAGCATTGCTCGACT
GTGGAGTAAAGTATGGCATACTGCGGCGCCTCCTAGAGCGCGGCATCGAGGTGACCCGCTACCCGTGTACGACTCCTGCC
TGGCAGCTCCTAGACGGCTACGACGCCGTGGTACTGAGTAACGGGCCCGGGAACCCCGCCCTCCTCCGAAGCCAGGCTAG
GACAGCAGCCGAGGTTGCCACCTCGGGTAAGCCAGTCCTAGCAATATGCCTCGGCATGCAGCTACTAGCTCTAGGCCTCG
GTGCACGCGCCTACAAGCTCCCCTACGGCCATCGCGGAGTAAACAAGCCGGTCGTCGAGCTTGGCACTGGCCGCTGCATG
GTTACCACGCACAACCACGGCTATGCTATTGACTGGGATAGCCTTGACGGAACCGGTCTCATACCGTGGTTCCGCCAGCC
CGATGACGGTACACTCGAAGGAGTACGTTCGCGTGACGGGCTAGTGGTAGCTACACAGTTCCACCCTGAGGCCGGGCCGG
GCCCCTGGGACTCCACATGGGTTTTCGACTTGTTCCTCAAGCTCATCGAGGAGACCAAGTCTCGACAAGCCGTCTAG

Upstream 100 bases:

>100_bases
AACGAGTAGAACATGACATGAAGCTCCTCCACTCGATTAGAACCAGCTTCGTGAACACGTGGGAAAACCTCGTAAAAACA
GCCAGAGAGGTGCTTGACGA

Downstream 100 bases:

>100_bases
CCCTGATGGTGCATGGCAAGGGGTGGCTGTGGAATGCCTAGGAGGATAGATGTTAGGAAGGTACTGATGCTGGGCAGCGG
CGCCATAAAGATAGCTGAGG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MQIGDIKVYRMPLLRCKTSLRARLLLADGVAVEGCGFGAEATRVGEVVFTTAMTGYPESLTDPSYRGQILVETHPMIGNY
GVPSRDRQLHGVPVDYESDHIQVEGFVVAELPQPSHYASVMSLHEWLRSEGVPGIYRVDTRFLVKRIREYGVVMGIISVY
RDGEEPPGWDELARKLVSSKSYDERIFALEVSPRKPIVHRPRGRVRARVALLDCGVKYGILRRLLERGIEVTRYPCTTPA
WQLLDGYDAVVLSNGPGNPALLRSQARTAAEVATSGKPVLAICLGMQLLALGLGARAYKLPYGHRGVNKPVVELGTGRCM
VTTHNHGYAIDWDSLDGTGLIPWFRQPDDGTLEGVRSRDGLVVATQFHPEAGPGPWDSTWVFDLFLKLIEETKSRQAV

Sequences:

>Translated_398_residues
MQIGDIKVYRMPLLRCKTSLRARLLLADGVAVEGCGFGAEATRVGEVVFTTAMTGYPESLTDPSYRGQILVETHPMIGNY
GVPSRDRQLHGVPVDYESDHIQVEGFVVAELPQPSHYASVMSLHEWLRSEGVPGIYRVDTRFLVKRIREYGVVMGIISVY
RDGEEPPGWDELARKLVSSKSYDERIFALEVSPRKPIVHRPRGRVRARVALLDCGVKYGILRRLLERGIEVTRYPCTTPA
WQLLDGYDAVVLSNGPGNPALLRSQARTAAEVATSGKPVLAICLGMQLLALGLGARAYKLPYGHRGVNKPVVELGTGRCM
VTTHNHGYAIDWDSLDGTGLIPWFRQPDDGTLEGVRSRDGLVVATQFHPEAGPGPWDSTWVFDLFLKLIEETKSRQAV
>Mature_398_residues
MQIGDIKVYRMPLLRCKTSLRARLLLADGVAVEGCGFGAEATRVGEVVFTTAMTGYPESLTDPSYRGQILVETHPMIGNY
GVPSRDRQLHGVPVDYESDHIQVEGFVVAELPQPSHYASVMSLHEWLRSEGVPGIYRVDTRFLVKRIREYGVVMGIISVY
RDGEEPPGWDELARKLVSSKSYDERIFALEVSPRKPIVHRPRGRVRARVALLDCGVKYGILRRLLERGIEVTRYPCTTPA
WQLLDGYDAVVLSNGPGNPALLRSQARTAAEVATSGKPVLAICLGMQLLALGLGARAYKLPYGHRGVNKPVVELGTGRCM
VTTHNHGYAIDWDSLDGTGLIPWFRQPDDGTLEGVRSRDGLVVATQFHPEAGPGPWDSTWVFDLFLKLIEETKSRQAV

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=382, Percent_Identity=43.1937172774869, Blast_Score=283, Evalue=1e-76,
Organism=Homo sapiens, GI21361331, Length=377, Percent_Identity=35.2785145888594, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI169790915, Length=377, Percent_Identity=35.2785145888594, Blast_Score=221, Evalue=1e-57,
Organism=Escherichia coli, GI1786215, Length=386, Percent_Identity=35.2331606217617, Blast_Score=210, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI193204318, Length=383, Percent_Identity=39.4255874673629, Blast_Score=250, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6324878, Length=378, Percent_Identity=42.3280423280423, Blast_Score=283, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6322331, Length=397, Percent_Identity=38.2871536523929, Blast_Score=270, Evalue=2e-73,
Organism=Drosophila melanogaster, GI45555749, Length=382, Percent_Identity=41.0994764397906, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24642586, Length=382, Percent_Identity=41.0994764397906, Blast_Score=252, Evalue=2e-67,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 44061; Mature: 44061

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGDIKVYRMPLLRCKTSLRARLLLADGVAVEGCGFGAEATRVGEVVFTTAMTGYPESL
CCCCCEEEEEHHHHHHHHHHHEEEEEECCEEEECCCCCCCHHHHHHEEEEEECCCCCCCC
TDPSYRGQILVETHPMIGNYGVPSRDRQLHGVPVDYESDHIQVEGFVVAELPQPSHYASV
CCCCCCCEEEEEECCCCCCCCCCCCCCEECCCCCCCCCCEEEEEEEEEEECCCCHHHHHH
MSLHEWLRSEGVPGIYRVDTRFLVKRIREYGVVMGIISVYRDGEEPPGWDELARKLVSSK
HHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
SYDERIFALEVSPRKPIVHRPRGRVRARVALLDCGVKYGILRRLLERGIEVTRYPCTTPA
CCCCEEEEEEECCCCCCCCCCCCHHEEEEEEECCCHHHHHHHHHHHCCCCEEECCCCCHH
WQLLDGYDAVVLSNGPGNPALLRSQARTAAEVATSGKPVLAICLGMQLLALGLGARAYKL
HHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEC
PYGHRGVNKPVVELGTGRCMVTTHNHGYAIDWDSLDGTGLIPWFRQPDDGTLEGVRSRDG
CCCCCCCCCCCEEECCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCC
LVVATQFHPEAGPGPWDSTWVFDLFLKLIEETKSRQAV
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQIGDIKVYRMPLLRCKTSLRARLLLADGVAVEGCGFGAEATRVGEVVFTTAMTGYPESL
CCCCCEEEEEHHHHHHHHHHHEEEEEECCEEEECCCCCCCHHHHHHEEEEEECCCCCCCC
TDPSYRGQILVETHPMIGNYGVPSRDRQLHGVPVDYESDHIQVEGFVVAELPQPSHYASV
CCCCCCCEEEEEECCCCCCCCCCCCCCEECCCCCCCCCCEEEEEEEEEEECCCCHHHHHH
MSLHEWLRSEGVPGIYRVDTRFLVKRIREYGVVMGIISVYRDGEEPPGWDELARKLVSSK
HHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
SYDERIFALEVSPRKPIVHRPRGRVRARVALLDCGVKYGILRRLLERGIEVTRYPCTTPA
CCCCEEEEEEECCCCCCCCCCCCHHEEEEEEECCCHHHHHHHHHHHCCCCEEECCCCCHH
WQLLDGYDAVVLSNGPGNPALLRSQARTAAEVATSGKPVLAICLGMQLLALGLGARAYKL
HHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEC
PYGHRGVNKPVVELGTGRCMVTTHNHGYAIDWDSLDGTGLIPWFRQPDDGTLEGVRSRDG
CCCCCCCCCCCEEECCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCC
LVVATQFHPEAGPGPWDSTWVFDLFLKLIEETKSRQAV
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA