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The map label for this gene is argH [H]

Identifier: 124027201

GI number: 124027201

Start: 291936

End: 293333

Strand: Reverse

Name: argH [H]

Synonym: Hbut_0304

Alternate gene names: 124027201

Gene position: 293333-291936 (Counterclockwise)

Preceding gene: 124027202

Following gene: 124027200

Centisome position: 17.59

GC content: 57.37

Gene sequence:

>1398_bases
TTGCTTAGGTACCGAGAGACCGTTGGCCTCAGCGGAGACTCTGCAATAGTCTACGACTACACCTCGAGCATAGGCCACGA
CAATGAAATATACACTTATGTAGTATACATCCTTATCGCCCATACGCTCCACCTGTACCGCCGCGGGATAATCCCCCGTA
GGGCTGCATGCCTAACACTACGTGTCCTCCTGGAGGCCCTCGAAAAGAAGCCTGGTGAACTGCTAGGGCCCGGATACGAG
GATGTGTTTGAAGCGCTTGAGGATTGGCTTGCACAGAAAACCGGGGGCGAATCAGCCTACATTTGGATTGGCAGGAGCCG
AAACGACCACGTCTCAGCCGCTCTCCGCCTCTACACGATCAATAAGATTATCGACACTCTATACTGGCTTCTCCAGGCAC
GTCTCCGCCTCATCGAGCTAGCAGAGAAGCATCGCGGTGCCCCCGTTCTATTCCATACGCATCAGCAGCCGAGCCAGCTT
GCAACCCTTGACTGTCTCCTTCTCGCCTGGGAGGAGGCGCTCGCCTCCGCCTACAAACTACTCTACAGCGTCATCGGTCT
CGTCGATAGAAGCCCCCTTGGCGCCAGCGCTGGAGCCGGGACACTAGCACCAATAGACCCCGAGGAGCTCGCCCGCCTCA
CCGGGTTTAGTGGCGTCCTCGGGAATAGCCTCTACGCTACTGGCAGCAGGTTGGACGTTACGGCCGCCGCGCTAGCAGCA
ACAGCACTGCTTGTCGAGGCTTCACGCATAGCGGAGAACCTTGTGCTACTTTCCACCCCGTACGTATCAGCTGTCCGCAT
ACCAGAGAGCCACGTTGCAACCAGCAGTATTATGCCGCACAAGAGGAACCCTGTCACCCTAGAAGTGCTTCGTGCGCGTG
CAGCCCGAGCAGCAGGCCTTGCAGCTGGCCTCCTAGCCATACAGAAGGGGCTCCCCTACGGCTATAACCTCGACCTGCAG
GAGGCAAACCCGCTCCTCTACACGATCCTGCGCGACGCAGCGGAGTCCAGCCGCATACTCGCAGACCTATTCTCCAGCAT
GGAGTTCGACCTGGAGAGACTCAGCAAGCTTGCAGAAAGCTACATTGCATGGGGCGCTGAGCTGGCCGAACTCCTCGCGC
TTAGGACTGGCATGCCTCTCCGCGAAGCCTACCGCGAAGTCGCTATGAAGCTCCGAGACGGTAAGATCAGCGAGCTCTTC
CAGAAGCTAGGCGTAACCAGTCCATCAGAGCTAACCTCCATGAGGCACACCGGGTGCAGGGCTGACCTAGACATGGCTAA
GCCGCGTGAACGAGTAGAACATGACATGAAGCTCCTCCACTCGATTAGAACCAGCTTCGTGAACACGTGGGAAAACCTCG
TAAAAACAGCCAGAGAGGTGCTTGACGAGTGCAAATAG

Upstream 100 bases:

>100_bases
CGCGCAGCCTCTGGGGCTAACCTGGCTGCAATGCCTGCTGGTGGTCTGCGGGTTCAGTAAGATTGATAATAGGACGGTAC
GCCGCGAGGGTGGTGGGTTA

Downstream 100 bases:

>100_bases
GCGATATAAAAGTCTATAGAATGCCCCTCCTTAGGTGTAAGACGAGTCTGCGTGCAAGGCTGCTACTAGCCGATGGTGTT
GCTGTTGAGGGCTGCGGCTT

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL; Arginosuccinase [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MLRYRETVGLSGDSAIVYDYTSSIGHDNEIYTYVVYILIAHTLHLYRRGIIPRRAACLTLRVLLEALEKKPGELLGPGYE
DVFEALEDWLAQKTGGESAYIWIGRSRNDHVSAALRLYTINKIIDTLYWLLQARLRLIELAEKHRGAPVLFHTHQQPSQL
ATLDCLLLAWEEALASAYKLLYSVIGLVDRSPLGASAGAGTLAPIDPEELARLTGFSGVLGNSLYATGSRLDVTAAALAA
TALLVEASRIAENLVLLSTPYVSAVRIPESHVATSSIMPHKRNPVTLEVLRARAARAAGLAAGLLAIQKGLPYGYNLDLQ
EANPLLYTILRDAAESSRILADLFSSMEFDLERLSKLAESYIAWGAELAELLALRTGMPLREAYREVAMKLRDGKISELF
QKLGVTSPSELTSMRHTGCRADLDMAKPRERVEHDMKLLHSIRTSFVNTWENLVKTAREVLDECK

Sequences:

>Translated_465_residues
MLRYRETVGLSGDSAIVYDYTSSIGHDNEIYTYVVYILIAHTLHLYRRGIIPRRAACLTLRVLLEALEKKPGELLGPGYE
DVFEALEDWLAQKTGGESAYIWIGRSRNDHVSAALRLYTINKIIDTLYWLLQARLRLIELAEKHRGAPVLFHTHQQPSQL
ATLDCLLLAWEEALASAYKLLYSVIGLVDRSPLGASAGAGTLAPIDPEELARLTGFSGVLGNSLYATGSRLDVTAAALAA
TALLVEASRIAENLVLLSTPYVSAVRIPESHVATSSIMPHKRNPVTLEVLRARAARAAGLAAGLLAIQKGLPYGYNLDLQ
EANPLLYTILRDAAESSRILADLFSSMEFDLERLSKLAESYIAWGAELAELLALRTGMPLREAYREVAMKLRDGKISELF
QKLGVTSPSELTSMRHTGCRADLDMAKPRERVEHDMKLLHSIRTSFVNTWENLVKTAREVLDECK
>Mature_465_residues
MLRYRETVGLSGDSAIVYDYTSSIGHDNEIYTYVVYILIAHTLHLYRRGIIPRRAACLTLRVLLEALEKKPGELLGPGYE
DVFEALEDWLAQKTGGESAYIWIGRSRNDHVSAALRLYTINKIIDTLYWLLQARLRLIELAEKHRGAPVLFHTHQQPSQL
ATLDCLLLAWEEALASAYKLLYSVIGLVDRSPLGASAGAGTLAPIDPEELARLTGFSGVLGNSLYATGSRLDVTAAALAA
TALLVEASRIAENLVLLSTPYVSAVRIPESHVATSSIMPHKRNPVTLEVLRARAARAAGLAAGLLAIQKGLPYGYNLDLQ
EANPLLYTILRDAAESSRILADLFSSMEFDLERLSKLAESYIAWGAELAELLALRTGMPLREAYREVAMKLRDGKISELF
QKLGVTSPSELTSMRHTGCRADLDMAKPRERVEHDMKLLHSIRTSFVNTWENLVKTAREVLDECK

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI31541964, Length=377, Percent_Identity=29.7082228116711, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI68303542, Length=377, Percent_Identity=29.7082228116711, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI68303549, Length=377, Percent_Identity=28.3819628647215, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI68303547, Length=377, Percent_Identity=26.2599469496021, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1790398, Length=340, Percent_Identity=32.6470588235294, Blast_Score=156, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6321806, Length=310, Percent_Identity=31.6129032258064, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI221473854, Length=311, Percent_Identity=29.5819935691318, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI78706858, Length=311, Percent_Identity=29.5819935691318, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF00206 Lyase_1 [H]

EC number: =4.3.2.1 [H]

Molecular weight: Translated: 51454; Mature: 51454

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRYRETVGLSGDSAIVYDYTSSIGHDNEIYTYVVYILIAHTLHLYRRGIIPRRAACLTL
CCCCCCCCCCCCCCEEEEECHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RVLLEALEKKPGELLGPGYEDVFEALEDWLAQKTGGESAYIWIGRSRNDHVSAALRLYTI
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHH
NKIIDTLYWLLQARLRLIELAEKHRGAPVLFHTHQQPSQLATLDCLLLAWEEALASAYKL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
LYSVIGLVDRSPLGASAGAGTLAPIDPEELARLTGFSGVLGNSLYATGSRLDVTAAALAA
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHH
TALLVEASRIAENLVLLSTPYVSAVRIPESHVATSSIMPHKRNPVTLEVLRARAARAAGL
HHHHHHHHHHHHCEEEEECCCHHEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
AAGLLAIQKGLPYGYNLDLQEANPLLYTILRDAAESSRILADLFSSMEFDLERLSKLAES
HHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIAWGAELAELLALRTGMPLREAYREVAMKLRDGKISELFQKLGVTSPSELTSMRHTGCR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCC
ADLDMAKPRERVEHDMKLLHSIRTSFVNTWENLVKTAREVLDECK
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLRYRETVGLSGDSAIVYDYTSSIGHDNEIYTYVVYILIAHTLHLYRRGIIPRRAACLTL
CCCCCCCCCCCCCCEEEEECHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RVLLEALEKKPGELLGPGYEDVFEALEDWLAQKTGGESAYIWIGRSRNDHVSAALRLYTI
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHH
NKIIDTLYWLLQARLRLIELAEKHRGAPVLFHTHQQPSQLATLDCLLLAWEEALASAYKL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
LYSVIGLVDRSPLGASAGAGTLAPIDPEELARLTGFSGVLGNSLYATGSRLDVTAAALAA
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHH
TALLVEASRIAENLVLLSTPYVSAVRIPESHVATSSIMPHKRNPVTLEVLRARAARAAGL
HHHHHHHHHHHHCEEEEECCCHHEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
AAGLLAIQKGLPYGYNLDLQEANPLLYTILRDAAESSRILADLFSSMEFDLERLSKLAES
HHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIAWGAELAELLALRTGMPLREAYREVAMKLRDGKISELFQKLGVTSPSELTSMRHTGCR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCC
ADLDMAKPRERVEHDMKLLHSIRTSFVNTWENLVKTAREVLDECK
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10701121; 11427726 [H]