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The map label for this gene is rutB [H]

Identifier: 124026965

GI number: 124026965

Start: 50853

End: 51485

Strand: Direct

Name: rutB [H]

Synonym: Hbut_0063

Alternate gene names: 124026965

Gene position: 50853-51485 (Clockwise)

Preceding gene: 124026962

Following gene: 124026966

Centisome position: 3.05

GC content: 50.55

Gene sequence:

>633_bases
ATGATTATCAACAAGAAGGTTGTCGAGGTGCCTGAGATACCGGTAGAGCAGACTGTAAGACTGTCGGCAGACAGGACAGC
AGTGATAGTGGTTGACATGCAGAACGATTTTGTGAGGCCGGAGGGCAAGCTATTCGTCCCAACAGCGCCCAAGACTATCC
AGGCGATAAGGGAGCTGCTCGAAAAGGCTAGGCGCCATGCAGTAATGACTATATACACCATGGATACGCACTACTCTGAT
GATCCCGAGTTTAGGATATGGGGGGAACATGTTGTTAAGGGCTCCTGGGGCTGGCAGATAGTAGACGAGTTAAAGCCTAC
CAGGAACGAGATAGTAGTCGAGAAGACTAGGTATGATGGGTTCTACGGTACGCCGATAGATGATCTGCTACGTGTGCATG
GTATAGAGAATGTTGTTATTGTTGGGACAGTTGCAAACATATGTGTTCTCCATACAGCGGCAAGCGCAGCACTACGCTGG
TACAAGGTGTACGTGCCCATGGATGGTATCAGCGCGCTCAATGAGTTCGACTACTATCTAACACTCCGGCAGATAACCTT
CCTCTACCGCGGCGTGGTTGTGGAGAGCGTTAAAGGCATAGAGTTTGAGGGTCAGGGCAAGTGGCCCCGTTAG

Upstream 100 bases:

>100_bases
GCTACAGCCGGGCTTACCGGCAACCTATAAGCCTTAGAGGCTAGAGGGTAGACCAGAACTATACTACTCGGGAACTATAC
TACTCGGGGGCGAGTTCCAC

Downstream 100 bases:

>100_bases
CCGGATAGCTGAAGACGTGCTCCGTAAACCGGAAGCATGAGTTTAAAGCCAAGAAGCGGCTATACATCAACCATACAACC
ACGACTGCTGCAATATCAGC

Product: iisochorismatase family protein

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MIINKKVVEVPEIPVEQTVRLSADRTAVIVVDMQNDFVRPEGKLFVPTAPKTIQAIRELLEKARRHAVMTIYTMDTHYSD
DPEFRIWGEHVVKGSWGWQIVDELKPTRNEIVVEKTRYDGFYGTPIDDLLRVHGIENVVIVGTVANICVLHTAASAALRW
YKVYVPMDGISALNEFDYYLTLRQITFLYRGVVVESVKGIEFEGQGKWPR

Sequences:

>Translated_210_residues
MIINKKVVEVPEIPVEQTVRLSADRTAVIVVDMQNDFVRPEGKLFVPTAPKTIQAIRELLEKARRHAVMTIYTMDTHYSD
DPEFRIWGEHVVKGSWGWQIVDELKPTRNEIVVEKTRYDGFYGTPIDDLLRVHGIENVVIVGTVANICVLHTAASAALRW
YKVYVPMDGISALNEFDYYLTLRQITFLYRGVVVESVKGIEFEGQGKWPR
>Mature_210_residues
MIINKKVVEVPEIPVEQTVRLSADRTAVIVVDMQNDFVRPEGKLFVPTAPKTIQAIRELLEKARRHAVMTIYTMDTHYSD
DPEFRIWGEHVVKGSWGWQIVDELKPTRNEIVVEKTRYDGFYGTPIDDLLRVHGIENVVIVGTVANICVLHTAASAALRW
YKVYVPMDGISALNEFDYYLTLRQITFLYRGVVVESVKGIEFEGQGKWPR

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=162, Percent_Identity=32.7160493827161, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786811, Length=166, Percent_Identity=24.6987951807229, Blast_Score=69, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321401, Length=185, Percent_Identity=29.7297297297297, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 24064; Mature: 24064

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIINKKVVEVPEIPVEQTVRLSADRTAVIVVDMQNDFVRPEGKLFVPTAPKTIQAIRELL
CCCCCCEEECCCCCHHHEEEECCCCEEEEEEECCCCEECCCCCEEECCCCHHHHHHHHHH
EKARRHAVMTIYTMDTHYSDDPEFRIWGEHVVKGSWGWQIVDELKPTRNEIVVEKTRYDG
HHHHHCEEEEEEEEECCCCCCCCEEEECCEEEECCCCCEEHHHCCCCCCCEEEEEEECCC
FYGTPIDDLLRVHGIENVVIVGTVANICVLHTAASAALRWYKVYVPMDGISALNEFDYYL
CCCCCHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHH
TLRQITFLYRGVVVESVKGIEFEGQGKWPR
HHHHHHHHHHHHHHHHHCCCEECCCCCCCC
>Mature Secondary Structure
MIINKKVVEVPEIPVEQTVRLSADRTAVIVVDMQNDFVRPEGKLFVPTAPKTIQAIRELL
CCCCCCEEECCCCCHHHEEEECCCCEEEEEEECCCCEECCCCCEEECCCCHHHHHHHHHH
EKARRHAVMTIYTMDTHYSDDPEFRIWGEHVVKGSWGWQIVDELKPTRNEIVVEKTRYDG
HHHHHCEEEEEEEEECCCCCCCCEEEECCEEEECCCCCEEHHHCCCCCCCEEEEEEECCC
FYGTPIDDLLRVHGIENVVIVGTVANICVLHTAASAALRWYKVYVPMDGISALNEFDYYL
CCCCCHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHH
TLRQITFLYRGVVVESVKGIEFEGQGKWPR
HHHHHHHHHHHHHHHHHCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA