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The map label for this gene is gldA [C]

Identifier: 123966411

GI number: 123966411

Start: 1033375

End: 1034469

Strand: Reverse

Name: gldA [C]

Synonym: P9515_11781

Alternate gene names: 123966411

Gene position: 1034469-1033375 (Counterclockwise)

Preceding gene: 123966412

Following gene: 123966410

Centisome position: 60.7

GC content: 32.79

Gene sequence:

>1095_bases
ATGCAGTCAATATCACCAGAAAAAATATTTAGGGGGAGTGGAGCTTGGATAAAAGCTATACCACATATTAAAAGTATTTC
AAAACGCCCATTACTTTTAGGAAGAAGTTTAGTAACTAATAATATTAGGCAGCAAATCTATAAAGATTTGCTTAGCGAAA
ACCTTGATACACATATTTCTAACCTGCAATTCGATTGTTGTTATGAAGATCTCAAAAGGGTAAAAAACATTGCACTAAAA
AATAATTGTGATTCTATTATTGCCATAGGAGGAGGTAAAGTCTTAGATTCCGGGAAATTTTTAGCAGATTCTCTTTCCAT
CCCAGTTATTACTATTCCCTTAAGTGCATCTACTTGTGCAGGCTGGACTGCACTATCAAACATTTATTCAACAACTGGGC
AATTCATTAAGGATATTGAGTTGAAATCATGTCCTAAAATTTTAGTATTTGATCATGCCTTTATTAAAACAGCTCCAAAG
AGGACACTTTCTAGCGGAATAGCTGACTCTTTGGCCAAATGGTATGAATCTTCTTTAACTAGTTCAAAAGTTAAAGATGG
ACTTGTCCAGCAAGCAATTCAAATATCACGAGTATTAAGAGATCAGCTTCTTATTGATGGAGAGAGTGCTTATCAGAGTC
AATCTCTTCAAAATACTTCTTGGTGCAATGTAGTTGAAGGATGTGCACTTACTGCGGGATTGATAGGAGGAATAGGAGGA
GAAAAGTGTAGAACGGCAGCTGCTCATGCTTTCCATAATGCTATCACTCAAATAGTTTCAACACAAAAGCCCTTACATGG
CGAAATTGTTGGAGTTGGAATATTATTGCAATTAAGACTAGAAGAAACCAAAAATAATAATAAATTAGCTAACCAATCTA
TTAATCAATTATTGGTATTAATGAAGAAATTAAATTTACCAACTACTATCGCTCAATTAGGTATAAATGTTTTTGAAAAT
AATAATTTACAGAGAATTGCCGATTTTACATGCAGGGATAAATCTGAGATTCATTTTTTACCTTTTAAAATCAGTCAACA
AGATATTATTGAAGTTATTTCGAATTTTGAAAGGCAAGAAATTAAGATTTCATAA

Upstream 100 bases:

>100_bases
TAGTCGATATTGATGAAAATAAGAAAGTTACAGTAAATATTTCCTCAGAAGAATCTTCTCAAGAATTAGCAGGAGCTAAT
TTTTAATAATCTAGGTGCAT

Downstream 100 bases:

>100_bases
ATTCTTTGAACAAAAATCTTTATAAAGACTTCAATAAATTTAATATTGATAATTTTGAAAAAATTAAATTTTCTATTCAA
GATCCATTAGGCCAAAAAAT

Product: putative glycerol dehydrogenase

Products: glycerone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MQSISPEKIFRGSGAWIKAIPHIKSISKRPLLLGRSLVTNNIRQQIYKDLLSENLDTHISNLQFDCCYEDLKRVKNIALK
NNCDSIIAIGGGKVLDSGKFLADSLSIPVITIPLSASTCAGWTALSNIYSTTGQFIKDIELKSCPKILVFDHAFIKTAPK
RTLSSGIADSLAKWYESSLTSSKVKDGLVQQAIQISRVLRDQLLIDGESAYQSQSLQNTSWCNVVEGCALTAGLIGGIGG
EKCRTAAAHAFHNAITQIVSTQKPLHGEIVGVGILLQLRLEETKNNNKLANQSINQLLVLMKKLNLPTTIAQLGINVFEN
NNLQRIADFTCRDKSEIHFLPFKISQQDIIEVISNFERQEIKIS

Sequences:

>Translated_364_residues
MQSISPEKIFRGSGAWIKAIPHIKSISKRPLLLGRSLVTNNIRQQIYKDLLSENLDTHISNLQFDCCYEDLKRVKNIALK
NNCDSIIAIGGGKVLDSGKFLADSLSIPVITIPLSASTCAGWTALSNIYSTTGQFIKDIELKSCPKILVFDHAFIKTAPK
RTLSSGIADSLAKWYESSLTSSKVKDGLVQQAIQISRVLRDQLLIDGESAYQSQSLQNTSWCNVVEGCALTAGLIGGIGG
EKCRTAAAHAFHNAITQIVSTQKPLHGEIVGVGILLQLRLEETKNNNKLANQSINQLLVLMKKLNLPTTIAQLGINVFEN
NNLQRIADFTCRDKSEIHFLPFKISQQDIIEVISNFERQEIKIS
>Mature_364_residues
MQSISPEKIFRGSGAWIKAIPHIKSISKRPLLLGRSLVTNNIRQQIYKDLLSENLDTHISNLQFDCCYEDLKRVKNIALK
NNCDSIIAIGGGKVLDSGKFLADSLSIPVITIPLSASTCAGWTALSNIYSTTGQFIKDIELKSCPKILVFDHAFIKTAPK
RTLSSGIADSLAKWYESSLTSSKVKDGLVQQAIQISRVLRDQLLIDGESAYQSQSLQNTSWCNVVEGCALTAGLIGGIGG
EKCRTAAAHAFHNAITQIVSTQKPLHGEIVGVGILLQLRLEETKNNNKLANQSINQLLVLMKKLNLPTTIAQLGINVFEN
NNLQRIADFTCRDKSEIHFLPFKISQQDIIEVISNFERQEIKIS

Specific function: Glycerol utilization. [C]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=344, Percent_Identity=29.9418604651163, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1786815, Length=278, Percent_Identity=33.4532374100719, Blast_Score=136, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.6

Molecular weight: Translated: 40103; Mature: 40103

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSISPEKIFRGSGAWIKAIPHIKSISKRPLLLGRSLVTNNIRQQIYKDLLSENLDTHIS
CCCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
NLQFDCCYEDLKRVKNIALKNNCDSIIAIGGGKVLDSGKFLADSLSIPVITIPLSASTCA
CCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEECCCCHHHHCCCCCEEEEECCCCHHH
GWTALSNIYSTTGQFIKDIELKSCPKILVFDHAFIKTAPKRTLSSGIADSLAKWYESSLT
HHHHHHHHHHHHHHHHHHCHHHCCCEEEEECHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
SSKVKDGLVQQAIQISRVLRDQLLIDGESAYQSQSLQNTSWCNVVEGCALTAGLIGGIGG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
EKCRTAAAHAFHNAITQIVSTQKPLHGEIVGVGILLQLRLEETKNNNKLANQSINQLLVL
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEHHCCCCCHHHHHHHHHHHHH
MKKLNLPTTIAQLGINVFENNNLQRIADFTCRDKSEIHFLPFKISQQDIIEVISNFERQE
HHHCCCCHHHHHHCCHHHCCCCHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHCCCHHC
IKIS
EECC
>Mature Secondary Structure
MQSISPEKIFRGSGAWIKAIPHIKSISKRPLLLGRSLVTNNIRQQIYKDLLSENLDTHIS
CCCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
NLQFDCCYEDLKRVKNIALKNNCDSIIAIGGGKVLDSGKFLADSLSIPVITIPLSASTCA
CCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEECCCCHHHHCCCCCEEEEECCCCHHH
GWTALSNIYSTTGQFIKDIELKSCPKILVFDHAFIKTAPKRTLSSGIADSLAKWYESSLT
HHHHHHHHHHHHHHHHHHCHHHCCCEEEEECHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
SSKVKDGLVQQAIQISRVLRDQLLIDGESAYQSQSLQNTSWCNVVEGCALTAGLIGGIGG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
EKCRTAAAHAFHNAITQIVSTQKPLHGEIVGVGILLQLRLEETKNNNKLANQSINQLLVL
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEHHCCCCCHHHHHHHHHHHHH
MKKLNLPTTIAQLGINVFENNNLQRIADFTCRDKSEIHFLPFKISQQDIIEVISNFERQE
HHHCCCCHHHHHHCCHHHCCCCHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHCCCHHC
IKIS
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.13 {NAD+}} 0.12 {NADH}} 0.06 {dihydroxyacetone}} 0.5 {glycerol}} [C]

Substrates: glycerol; NAD+

Specific reaction: glycerol + NAD+ = glycerone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Chelatingagents; Li+; Na+; Thiolcompounds [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]