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The map label for this gene is dacB [C]

Identifier: 123966200

GI number: 123966200

Start: 861497

End: 862717

Strand: Reverse

Name: dacB [C]

Synonym: P9515_09671

Alternate gene names: 123966200

Gene position: 862717-861497 (Counterclockwise)

Preceding gene: 123966201

Following gene: 123966198

Centisome position: 50.62

GC content: 22.19

Gene sequence:

>1221_bases
GTGATAAGAAAATTTTACTTTTTAATTGTTGTTATATCTTCTTTATTAAGTTTTCCATTTATATTAAGGTATCTAATAAT
TACAAAAAAAAGTAATTTTATTAATAATATTTATTTTAATTTGCCAGTAATTATTAATAATAAAAAAAAATGTCGAAATT
ATGATTTTTTATACAAATCACTTGATGAGACATATAGTGTATCAATTATTAACGAGAATGGAACTTTAATAAGTTCATAT
AATGATAAAATATTAAGGATTCCAGCTTCTAATTTAAAATTATTTTCCACTGCATATTTATTAAATAAATATAAATCATC
AAAAAATTTTAAAACATCAATATTTAGAAGAAATAGTAATAAATATTATTTATTAGGTAGCGGAGATCCAGATCTTAGTT
ATTCAGATATATATAAATTACTATCTAATATTTCTTATAATAAGAAAATTAATCTAAACATATTTGAAATAAATTCTAAA
TATTATTGGCCAAAAGGTTGGACATCTGGAGATAAAAAGTTTGAATATGGAGCTCCAATTACAACTTTGGCATTAAATAG
TAATCAGAGTAAATATGAAAATATAGAAACCTTAAAAAGAAGTATTGAAAAATATTTATTTAATAAATATCCATCCTCCT
CAATAAAAGTAAAAATTGTAGAAAATTCAAAAAAATTTTATATGAAACCTTCAATTGAATTGGATTCAATCAGATCTAAT
CCATTATTATCTTTAATAACATTAGCTAATTCTGAAAGTCATAATTTTACATCTGAATCACTATTTAAAAATGCATCAAA
TACCTGGAATGATAATAATTATTTTAAATTAAAAAATTGGTTGAGAAATATAGGGTTAAATGTAGCAAATTCATCATTTG
CAGATGCTAGTGGACTTTCTAGAAATAATAGAGTAAACACCAAATTAATAGCTGAATTTTTGAATAAGATGAAATATTCA
AAAGATTTTAGTACCTACCAATCCTCACTATCAATTATTGGAGTAAGAGGAACATTGGCTAAGAGATACTCAAACTCTGA
ATTACAAGGTAAATTTGTTGGAAAAACTGGGACTTTATCAAATGTATTTGCCTTATCAGGATACCTATTTAAAGATAACA
AATCTTTTGTTGTAAGTATTATTCAAAATTCAGAAAACATCGATAGAGATACTACATTTAACTTATTAAAAAATATTTAT
AAATTAGAAGGATGTTTTTAA

Upstream 100 bases:

>100_bases
TGGTTACTTTTTAAAGAAATTAACAACAAATATTTATTTAAAAAAGAATTTGAGGAGAAACCAGACAATGAAACAATTTT
TGTAAATTTATAGATTCTAA

Downstream 100 bases:

>100_bases
TTTTTTTTGAAATATTCCTTTAGATCTCTTTCCACAGCTTCTGGAACCATATGATTTATTTCTCCTCCAAATTTAGCAAC
TTCTTTAACCAAAGAACTAC

Product: D-Ala-D-Ala carboxypeptidase 3

Products: NA

Alternate protein names: Peptidase; Serine-Type D-Ala-D-Ala Carboxypeptidase; D-Ala-D-Ala Carboxypeptidase 3 Family Protein

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MIRKFYFLIVVISSLLSFPFILRYLIITKKSNFINNIYFNLPVIINNKKKCRNYDFLYKSLDETYSVSIINENGTLISSY
NDKILRIPASNLKLFSTAYLLNKYKSSKNFKTSIFRRNSNKYYLLGSGDPDLSYSDIYKLLSNISYNKKINLNIFEINSK
YYWPKGWTSGDKKFEYGAPITTLALNSNQSKYENIETLKRSIEKYLFNKYPSSSIKVKIVENSKKFYMKPSIELDSIRSN
PLLSLITLANSESHNFTSESLFKNASNTWNDNNYFKLKNWLRNIGLNVANSSFADASGLSRNNRVNTKLIAEFLNKMKYS
KDFSTYQSSLSIIGVRGTLAKRYSNSELQGKFVGKTGTLSNVFALSGYLFKDNKSFVVSIIQNSENIDRDTTFNLLKNIY
KLEGCF

Sequences:

>Translated_406_residues
MIRKFYFLIVVISSLLSFPFILRYLIITKKSNFINNIYFNLPVIINNKKKCRNYDFLYKSLDETYSVSIINENGTLISSY
NDKILRIPASNLKLFSTAYLLNKYKSSKNFKTSIFRRNSNKYYLLGSGDPDLSYSDIYKLLSNISYNKKINLNIFEINSK
YYWPKGWTSGDKKFEYGAPITTLALNSNQSKYENIETLKRSIEKYLFNKYPSSSIKVKIVENSKKFYMKPSIELDSIRSN
PLLSLITLANSESHNFTSESLFKNASNTWNDNNYFKLKNWLRNIGLNVANSSFADASGLSRNNRVNTKLIAEFLNKMKYS
KDFSTYQSSLSIIGVRGTLAKRYSNSELQGKFVGKTGTLSNVFALSGYLFKDNKSFVVSIIQNSENIDRDTTFNLLKNIY
KLEGCF
>Mature_406_residues
MIRKFYFLIVVISSLLSFPFILRYLIITKKSNFINNIYFNLPVIINNKKKCRNYDFLYKSLDETYSVSIINENGTLISSY
NDKILRIPASNLKLFSTAYLLNKYKSSKNFKTSIFRRNSNKYYLLGSGDPDLSYSDIYKLLSNISYNKKINLNIFEINSK
YYWPKGWTSGDKKFEYGAPITTLALNSNQSKYENIETLKRSIEKYLFNKYPSSSIKVKIVENSKKFYMKPSIELDSIRSN
PLLSLITLANSESHNFTSESLFKNASNTWNDNNYFKLKNWLRNIGLNVANSSFADASGLSRNNRVNTKLIAEFLNKMKYS
KDFSTYQSSLSIIGVRGTLAKRYSNSELQGKFVGKTGTLSNVFALSGYLFKDNKSFVVSIIQNSENIDRDTTFNLLKNIY
KLEGCF

Specific function: Not Involved In Transpeptidation But Exclusively Catalyzes A Dd-Carboxypeptidase And Dd-Endopeptidase Reaction. [C]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.4.16.4; 3.4.99.- [C]

Molecular weight: Translated: 46870; Mature: 46870

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKFYFLIVVISSLLSFPFILRYLIITKKSNFINNIYFNLPVIINNKKKCRNYDFLYKS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCHHHCCHHHHHHH
LDETYSVSIINENGTLISSYNDKILRIPASNLKLFSTAYLLNKYKSSKNFKTSIFRRNSN
CCCEEEEEEECCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCC
KYYLLGSGDPDLSYSDIYKLLSNISYNKKINLNIFEINSKYYWPKGWTSGDKKFEYGAPI
EEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEEEEECCEEECCCCCCCCCCCEECCCCE
TTLALNSNQSKYENIETLKRSIEKYLFNKYPSSSIKVKIVENSKKFYMKPSIELDSIRSN
EEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEECCCCCHHCCCCC
PLLSLITLANSESHNFTSESLFKNASNTWNDNNYFKLKNWLRNIGLNVANSSFADASGLS
CCEEEEEEECCCCCCCCHHHHHHCCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCC
RNNRVNTKLIAEFLNKMKYSKDFSTYQSSLSIIGVRGTLAKRYSNSELQGKFVGKTGTLS
CCCCCCHHHHHHHHHHHHCCCCHHHHHCCEEEEEECHHHHHHCCCCCCCEEEECCCCCHH
NVFALSGYLFKDNKSFVVSIIQNSENIDRDTTFNLLKNIYKLEGCF
HHHHHEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRKFYFLIVVISSLLSFPFILRYLIITKKSNFINNIYFNLPVIINNKKKCRNYDFLYKS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCHHHCCHHHHHHH
LDETYSVSIINENGTLISSYNDKILRIPASNLKLFSTAYLLNKYKSSKNFKTSIFRRNSN
CCCEEEEEEECCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCC
KYYLLGSGDPDLSYSDIYKLLSNISYNKKINLNIFEINSKYYWPKGWTSGDKKFEYGAPI
EEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEEEEECCEEECCCCCCCCCCCEECCCCE
TTLALNSNQSKYENIETLKRSIEKYLFNKYPSSSIKVKIVENSKKFYMKPSIELDSIRSN
EEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEECCCCCHHCCCCC
PLLSLITLANSESHNFTSESLFKNASNTWNDNNYFKLKNWLRNIGLNVANSSFADASGLS
CCEEEEEEECCCCCCCCHHHHHHCCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCC
RNNRVNTKLIAEFLNKMKYSKDFSTYQSSLSIIGVRGTLAKRYSNSELQGKFVGKTGTLS
CCCCCCHHHHHHHHHHHHCCCCHHHHHCCEEEEEECHHHHHHCCCCCCCEEEECCCCCHH
NVFALSGYLFKDNKSFVVSIIQNSENIDRDTTFNLLKNIYKLEGCF
HHHHHEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: Preferential cleavage is (Ac)2-L-Lys-D-Ala D-Ala and Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine [C]

General reaction: Hydrolase; Acting on peptide bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA