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The map label for this gene is vsr [H]

Identifier: 123965673

GI number: 123965673

Start: 388795

End: 389220

Strand: Direct

Name: vsr [H]

Synonym: P9515_04381

Alternate gene names: 123965673

Gene position: 388795-389220 (Clockwise)

Preceding gene: 123965671

Following gene: 123965684

Centisome position: 22.81

GC content: 28.87

Gene sequence:

>426_bases
ATGAGTAGTGAAGTAATACATAAGGTAAGTGAGCAGAGATCAAGGAATATGTCTGCCATTAAATCTAAAAATACAAAGCC
TGAAATCGCGGTAAGAAAACTTTTACATTCAATGGGATATAGATTCAGATTACATAGAAAAGATTTACCAGGTTCTCCTG
ATATTGTTCTGCCAAAATATAAAACAGCAATTTTTGTACATGGATGTTTTTGGCATAGGCATGCGAATTGTAAGTATGCA
TCAACTCCAAAAACAAGAAAAGAATTTTGGGAAAATAAATTTAGAGCAAATGTAAAAAGAGATTTGGAAATTCAGGAAAA
AATAAAAATTATGGGTTGGAAATCTGTCGTTATTTGGGAATGCGAAATTAAAAATAAATCTATCTTTGAAAAGAATTTTA
ATAATCTTTTTTTAGATGATTTTTAA

Upstream 100 bases:

>100_bases
TATAAATTACGTGGGTATGTGTGTAAACACATAATGCACGTACTGTATATAGGTACCTTTAGGGTGATGGTGATTATATT
GTTTAAAACTGATTGGTTAG

Downstream 100 bases:

>100_bases
ATAGTATCATTTTCAATCTTTTTATTTGGGATATATTTTTGTAAATTTAGAATTCTCTCCAGTAGGTTTTCTGGACTCTC
TTTATGAGCGCTAACTGATC

Product: hypothetical protein

Products: DNA [C]

Alternate protein names: V.XorII [H]

Number of amino acids: Translated: 141; Mature: 140

Protein sequence:

>141_residues
MSSEVIHKVSEQRSRNMSAIKSKNTKPEIAVRKLLHSMGYRFRLHRKDLPGSPDIVLPKYKTAIFVHGCFWHRHANCKYA
STPKTRKEFWENKFRANVKRDLEIQEKIKIMGWKSVVIWECEIKNKSIFEKNFNNLFLDDF

Sequences:

>Translated_141_residues
MSSEVIHKVSEQRSRNMSAIKSKNTKPEIAVRKLLHSMGYRFRLHRKDLPGSPDIVLPKYKTAIFVHGCFWHRHANCKYA
STPKTRKEFWENKFRANVKRDLEIQEKIKIMGWKSVVIWECEIKNKSIFEKNFNNLFLDDF
>Mature_140_residues
SSEVIHKVSEQRSRNMSAIKSKNTKPEIAVRKLLHSMGYRFRLHRKDLPGSPDIVLPKYKTAIFVHGCFWHRHANCKYAS
TPKTRKEFWENKFRANVKRDLEIQEKIKIMGWKSVVIWECEIKNKSIFEKNFNNLFLDDF

Specific function: May nick XorII sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=114, Percent_Identity=40.3508771929825, Blast_Score=107, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 16827; Mature: 16696

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEVIHKVSEQRSRNMSAIKSKNTKPEIAVRKLLHSMGYRFRLHRKDLPGSPDIVLPKY
CCHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEECCCC
KTAIFVHGCFWHRHANCKYASTPKTRKEFWENKFRANVKRDLEIQEKIKIMGWKSVVIWE
CEEEEEEEHEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHEEECCCEEEEEE
CEIKNKSIFEKNFNNLFLDDF
EECCCCHHHHCCCCCEECCCC
>Mature Secondary Structure 
SSEVIHKVSEQRSRNMSAIKSKNTKPEIAVRKLLHSMGYRFRLHRKDLPGSPDIVLPKY
CHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEECCCC
KTAIFVHGCFWHRHANCKYASTPKTRKEFWENKFRANVKRDLEIQEKIKIMGWKSVVIWE
CEEEEEEEHEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHEEECCCEEEEEE
CEIKNKSIFEKNFNNLFLDDF
EECCCCHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8078464 [H]