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The map label for this gene is trmE

Identifier: 123965453

GI number: 123965453

Start: 198356

End: 199741

Strand: Reverse

Name: trmE

Synonym: P9515_02181

Alternate gene names: 123965453

Gene position: 199741-198356 (Counterclockwise)

Preceding gene: 123965455

Following gene: 123965452

Centisome position: 11.72

GC content: 27.71

Gene sequence:

>1386_bases
ATGGAATCAATTGTCGATACTGAAGATACAATAGCAGCAATAGCTTCGGCTATAAGTCTAGGTAAAGGAGGAATTGCAGT
TATAAGAGTAACAGGGGAAGAAGCAATAAATTCCTGTAAAAATATCGTCGAAACAAAATCTAAATATGCTTGGGAGTCTC
ATAGAGTTTTTCATGGCTACGTTAAAGATAATTTAGAAAATAAATATATAGACGAAATTTTAATTTCAGTAATGCATTCT
CCTAATAGTTTCACAGGAGAAGATGTTGTTGAGTTACATTGTCATGGGGGAGTAATTGTTGTGAATAAAGTAATGGATAC
ATTATTATCAAACAATAATAAAGTGAGGATTGCGAATCCAGGAGAATTTAGTCAAAGAGCTTTTCTTAACGGCAAAATAG
ATTTAACTCAAGCAGAATCAATTAATCAATTAATTAATGCAAAAAATCTTAAATCAGCAGAGATAGCTTTTAATGGAGTA
AAGGGGGAAATCAAGAAAAAGATTGATGTTATTAAAAATGATTTAATCGAACAATTATCAGAAATAGAAGCAAGAGTCGA
TTTTGAAGAGGATTTTTCAGATTTTAATTATCATGATTTTTCTAGGAGTATTAAAAATATAAAAGAAAAAATAGAAATTC
TTATCGAAAATACAAAAAGGGGGGCATCAATTCATAATGGGATATCGATTGCTCTTATAGGCAAAACCAATGTTGGGAAA
AGTTCTCTTTTAAATCTTCTCTCAAAAAAAGAAAAAGCTATAGTTACTAATATCCCAGGAACAACTAGAGATATGATTGA
AGTCAATTTGACCATTAAAGATATCCCAATAAAATTAGTAGATACCGCTGGGATTAGAGACACAGATGAATATATAGAAA
ATATTGGAATAGAGAAAACCTTTGAAATGATTCAAAAATCAGATTATATTATTTATTTATACAGCCTAGAAGATGGATTT
AATAAAGATGATGAGGAAATTATAAGCAAAATTCCACGAGAAAAATTAATAACTATTTTGGGAAATAAAAAAGATTTAAT
TGATTCAAAAAAAAATGATCAAAAAACTTTTTCATCAGACATCGTTTTAATGAGTATCAAAAATAATGAAGGTGAAAAAG
ATTTAGTCGACAAAATAGTTAAAAAATGTGGATCAAAAGAATTTGAAAATATAGATATATTTTTAAATGAAAGACAAATA
TCAAACCTGAGGGATTGCCTAAAAAATTTAAATGATACTGATCCAATAATTGCTAATCAATTACCTTTCGATTTATTATC
GATTGAAGTTAGAGATGGGATAAAAAACTTATCTAGAATTACAGGTCAAGAATTAACTGAAGATCTACTAAATAATATTT
TTTCTAAATTTTGCATAGGTAAATAA

Upstream 100 bases:

>100_bases
TTTTTTTAAAGTTAAGATTTTTTATCAACTTCATTCTGAGATCTCAATCAAAAAAGTTTTCTTTGATAAGCCTACTTAAT
TATCCATAACATAGTGAGAG

Downstream 100 bases:

>100_bases
ATTTTTAATTAAATGACATAAATATATAAAGTAAACTTTAAAAGTTAAAATAAAATTCATTATCTATTACAAGTTTTGTG
GACTTTTACTCTCCAAACAT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MESIVDTEDTIAAIASAISLGKGGIAVIRVTGEEAINSCKNIVETKSKYAWESHRVFHGYVKDNLENKYIDEILISVMHS
PNSFTGEDVVELHCHGGVIVVNKVMDTLLSNNNKVRIANPGEFSQRAFLNGKIDLTQAESINQLINAKNLKSAEIAFNGV
KGEIKKKIDVIKNDLIEQLSEIEARVDFEEDFSDFNYHDFSRSIKNIKEKIEILIENTKRGASIHNGISIALIGKTNVGK
SSLLNLLSKKEKAIVTNIPGTTRDMIEVNLTIKDIPIKLVDTAGIRDTDEYIENIGIEKTFEMIQKSDYIIYLYSLEDGF
NKDDEEIISKIPREKLITILGNKKDLIDSKKNDQKTFSSDIVLMSIKNNEGEKDLVDKIVKKCGSKEFENIDIFLNERQI
SNLRDCLKNLNDTDPIIANQLPFDLLSIEVRDGIKNLSRITGQELTEDLLNNIFSKFCIGK

Sequences:

>Translated_461_residues
MESIVDTEDTIAAIASAISLGKGGIAVIRVTGEEAINSCKNIVETKSKYAWESHRVFHGYVKDNLENKYIDEILISVMHS
PNSFTGEDVVELHCHGGVIVVNKVMDTLLSNNNKVRIANPGEFSQRAFLNGKIDLTQAESINQLINAKNLKSAEIAFNGV
KGEIKKKIDVIKNDLIEQLSEIEARVDFEEDFSDFNYHDFSRSIKNIKEKIEILIENTKRGASIHNGISIALIGKTNVGK
SSLLNLLSKKEKAIVTNIPGTTRDMIEVNLTIKDIPIKLVDTAGIRDTDEYIENIGIEKTFEMIQKSDYIIYLYSLEDGF
NKDDEEIISKIPREKLITILGNKKDLIDSKKNDQKTFSSDIVLMSIKNNEGEKDLVDKIVKKCGSKEFENIDIFLNERQI
SNLRDCLKNLNDTDPIIANQLPFDLLSIEVRDGIKNLSRITGQELTEDLLNNIFSKFCIGK
>Mature_461_residues
MESIVDTEDTIAAIASAISLGKGGIAVIRVTGEEAINSCKNIVETKSKYAWESHRVFHGYVKDNLENKYIDEILISVMHS
PNSFTGEDVVELHCHGGVIVVNKVMDTLLSNNNKVRIANPGEFSQRAFLNGKIDLTQAESINQLINAKNLKSAEIAFNGV
KGEIKKKIDVIKNDLIEQLSEIEARVDFEEDFSDFNYHDFSRSIKNIKEKIEILIENTKRGASIHNGISIALIGKTNVGK
SSLLNLLSKKEKAIVTNIPGTTRDMIEVNLTIKDIPIKLVDTAGIRDTDEYIENIGIEKTFEMIQKSDYIIYLYSLEDGF
NKDDEEIISKIPREKLITILGNKKDLIDSKKNDQKTFSSDIVLMSIKNNEGEKDLVDKIVKKCGSKEFENIDIFLNERQI
SNLRDCLKNLNDTDPIIANQLPFDLLSIEVRDGIKNLSRITGQELTEDLLNNIFSKFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=464, Percent_Identity=28.8793103448276, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI193082993, Length=453, Percent_Identity=29.1390728476821, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI193082988, Length=496, Percent_Identity=28.0241935483871, Blast_Score=191, Evalue=1e-48,
Organism=Escherichia coli, GI2367268, Length=465, Percent_Identity=31.1827956989247, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI87082120, Length=121, Percent_Identity=32.2314049586777, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17507259, Length=466, Percent_Identity=29.8283261802575, Blast_Score=164, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6323665, Length=507, Percent_Identity=29.1913214990138, Blast_Score=180, Evalue=3e-46,
Organism=Drosophila melanogaster, GI45550813, Length=465, Percent_Identity=29.0322580645161, Blast_Score=191, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_PROM5 (A2BUG6)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010534.1
- ProteinModelPortal:   A2BUG6
- SMR:   A2BUG6
- STRING:   A2BUG6
- GeneID:   4720354
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_02181
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   PMAR167542:P9515ORF_0225-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00449
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51787; Mature: 51787

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: BINDING 29-29 BINDING 91-91 BINDING 132-132 BINDING 461-461

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIVDTEDTIAAIASAISLGKGGIAVIRVTGEEAINSCKNIVETKSKYAWESHRVFHGY
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCEEEHH
VKDNLENKYIDEILISVMHSPNSFTGEDVVELHCHGGVIVVNKVMDTLLSNNNKVRIANP
HHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEHHHHHHHHHCCCCEEEEECC
GEFSQRAFLNGKIDLTQAESINQLINAKNLKSAEIAFNGVKGEIKKKIDVIKNDLIEQLS
CCCCHHHEECCCEEEHHHHHHHHHHHHCCCCCHHEEECCCHHHHHHHHHHHHHHHHHHHH
EIEARVDFEEDFSDFNYHDFSRSIKNIKEKIEILIENTKRGASIHNGISIALIGKTNVGK
HHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCH
SSLLNLLSKKEKAIVTNIPGTTRDMIEVNLTIKDIPIKLVDTAGIRDTDEYIENIGIEKT
HHHHHHHHHCCCEEEECCCCCCCCEEEEEEEEEECCEEEEECCCCCCHHHHHHHCCHHHH
FEMIQKSDYIIYLYSLEDGFNKDDEEIISKIPREKLITILGNKKDLIDSKKNDQKTFSSD
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHCCHHHHHHHCCCCHHHHCCCCCCCHHHCCC
IVLMSIKNNEGEKDLVDKIVKKCGSKEFENIDIFLNERQISNLRDCLKNLNDTDPIIANQ
EEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCC
LPFDLLSIEVRDGIKNLSRITGQELTEDLLNNIFSKFCIGK
CCHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MESIVDTEDTIAAIASAISLGKGGIAVIRVTGEEAINSCKNIVETKSKYAWESHRVFHGY
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCEEEHH
VKDNLENKYIDEILISVMHSPNSFTGEDVVELHCHGGVIVVNKVMDTLLSNNNKVRIANP
HHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEHHHHHHHHHCCCCEEEEECC
GEFSQRAFLNGKIDLTQAESINQLINAKNLKSAEIAFNGVKGEIKKKIDVIKNDLIEQLS
CCCCHHHEECCCEEEHHHHHHHHHHHHCCCCCHHEEECCCHHHHHHHHHHHHHHHHHHHH
EIEARVDFEEDFSDFNYHDFSRSIKNIKEKIEILIENTKRGASIHNGISIALIGKTNVGK
HHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCH
SSLLNLLSKKEKAIVTNIPGTTRDMIEVNLTIKDIPIKLVDTAGIRDTDEYIENIGIEKT
HHHHHHHHHCCCEEEECCCCCCCCEEEEEEEEEECCEEEEECCCCCCHHHHHHHCCHHHH
FEMIQKSDYIIYLYSLEDGFNKDDEEIISKIPREKLITILGNKKDLIDSKKNDQKTFSSD
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHCCHHHHHHHCCCCHHHHCCCCCCCHHHCCC
IVLMSIKNNEGEKDLVDKIVKKCGSKEFENIDIFLNERQISNLRDCLKNLNDTDPIIANQ
EEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCC
LPFDLLSIEVRDGIKNLSRITGQELTEDLLNNIFSKFCIGK
CCHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA