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The map label for this gene is spoT [H]

Identifier: 123965455

GI number: 123965455

Start: 200279

End: 202588

Strand: Reverse

Name: spoT [H]

Synonym: P9515_02201

Alternate gene names: 123965455

Gene position: 202588-200279 (Counterclockwise)

Preceding gene: 123965457

Following gene: 123965453

Centisome position: 11.89

GC content: 33.16

Gene sequence:

>2310_bases
ATGTCTGAGGCAACTGCGGATTCAAAAGAGAGAAAAGAAATTGAAGTATCAACAATCATTGTGCCTGAAAATAAAAATTA
TGAAAGTGAATCTTTGAAGTATGAGATAAAAATTCCAAATTGGCTGCTTGAAAATATTCAAAACTATGAACTATCAAATA
ATAAAAATGATTCTAATCAAGATCTTATAGTCAAAGCTTTCAAGCTTGCTTACCAAGCTCATAATGGACAACTTAGGGCA
AGTGGTGAGCCATATATAATTCATCCAATTGCTGTTGCAGATCTTCTTAAAGAAATAGGGGCAAGTTCATCCGTTATTGC
TGCTGGATTATTACATGATGTAGTTGAAGATACAGGAATAGCTCTTTCGGAGATAGAAATTAATTTTGGCTTAGAAGTAA
AAGTACTTGTAGAGGGTGTAACTAAATTAGGTGGTATTCACTTTAATAATAGAACTGAAGCGCAAGCTGAAAATCTTAGA
AAAATGTTCTTAGCCATGGCAAGTGACATCAGAGTTGTTTTAGTTAAACTTGCAGATCGACTTCATAATATGAGAACAAT
TCAATGGCTTAATGAAGAGAGAAAAGAGAGAATTGCAAGAGAAACGAGAGAAATATATGCACCTTTAGCAAACAGGTTAG
GAATAAATAGATTCAAATGGGAATTAGAAGATTTGGCATTTAAATTTCTTGAACCAGATGAATATAAAAATTTAAAAGAT
CAAATTGTAGTTAAAAGAAGTGATAGAGAAAAAAGATTAGATAATACTCTAAATTTAATGAAAGAAAATTTAGTCTCATC
AGGTTTAAAAAATTTTGAAATTACAGGAAGACCAAAACATCTTTACGGTATCTGGAGCAAAATGGAAAGACAGCAAAAAC
AATTTAGCGAGATTTATGACGTTGCTGCTTTAAGAATTATTGTTAGCAATTTAGATAGCTGTTATAAAGCTTTAGCAGTC
GTTCATGATACTTTTAGACCAATCCCTGGAAGATTTAAAGATTACATAGGCTTACCAAAACCTAATGGCTACCAGTCCTT
ACATACTTCTGTTATTGGCAAACATAGGCCTATTGAAGTTCAAATCAGAACAACTTCAATGCATCAAATTGCAGAATTTG
GAATAGCTGCACATTGGCAATATAAAGAAGGTGGTTCGCCTGCATCTAGTAATGCAGAGAGATTTAATTGGCTTAGACAA
CTAGTAGAATGGCAACAAGAAGGAAATGAGAAAGATCATAATGACTATTTAGCATCAATAAAAGAAGATTTATTCGATGA
AGAAGTTTTTGTGATTACACCAAAAGGTGACGTTGTTGGTCTAAGGAAAGGATCTACTGCAATAGATTTTGCTTACAGAA
TACATTCAGAAATAGGTAACCATTGTAATGGGATAAGAATCAATGAAAAGCTATCTCCTCTATCTACATCACTACAAAAT
GGGGACTTTATAGAAATTTTGACTAATAATAATTCAACTCCAAGCCTAGACTGGTTGAATTTTGTAGTTACTCCAACCGC
CAAAAATAGAATTCGGCAGTGGTATAAGAAAAGCCATAGAGATGAGACTATCAAGCGAGGGAAAGACCTACTTGAGAAAG
AGATAGGTCGTAATGGATTTGAATCTTTACTTTCAAGTGATGCAATGCAAAAAGTTGCTCATCGATGCAATTTAAAGACA
ACAGAAGATCTTCTCGCATCATTAGGATTTGGTGGATTAACTTTGCATCAAGTATTAAATCGACTAAGAGAAGAAATTAA
AATACAAACAGAAGAAATTAAAAATGAATCCAATGCTGAAATAGCAAAAACACTTGTCAATAAAAATAATTCAATAACAA
CTAAATCTCATGCAACTAATAAATCACCAATCTCTGGGGTCGAAGGCCTAGATTATAGAATCGGAAAATGCTGCAGTCCA
CTTCCCGGGGAGGAAATAATTGGAACTGTCTCTTTAGGTAATCATGGCATCACTATACATAGAAGAGATTGCGAGAACGT
TCTCCCAATACCAATTGAGAGAAGATTACCTGTTGCATGGAACCAAGAGAATAAAGTTCTTGACAATAAGTTTCCAATTC
AACTCAGAATAGAAGTTATAGATAGAGTAGGTGTCCTAAAAGATATTCTTATGAGATTATCTGATAAAGGAATAAATGTT
AGTGATGCAAATGTAAAAACCGCTTTTGGTAAACCTGCCATAATTAATTTATGTGTTGGTCTAGAAAGTTATAGCCAACT
CCACAAAACTATTGATCAAATTAAATCAATGGCTGATGTATTAGATATTGCGAGAGTAGGAATAAGTTAA

Upstream 100 bases:

>100_bases
ATTTAGATTCCTGACTTCAAGAATTTTGTTTTTATTTATTTTCATAATTTTATAGCAAATTTCTCTGGTCCTAAATAAAA
TAAATCTAAAGCAATATTAT

Downstream 100 bases:

>100_bases
TTTAAACTCAAAATAACTTTACAATACTCTTTTTTTAATTTTTAATTTATATAGCAAAAAAATAATCATCCCAGAAATAG
AAGCTAATAGTAATCCAACT

Product: guanosine-3',5'-bis(diphosphate) 3'-diphosphatase, (ppGpp)ase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 769; Mature: 768

Protein sequence:

>769_residues
MSEATADSKERKEIEVSTIIVPENKNYESESLKYEIKIPNWLLENIQNYELSNNKNDSNQDLIVKAFKLAYQAHNGQLRA
SGEPYIIHPIAVADLLKEIGASSSVIAAGLLHDVVEDTGIALSEIEINFGLEVKVLVEGVTKLGGIHFNNRTEAQAENLR
KMFLAMASDIRVVLVKLADRLHNMRTIQWLNEERKERIARETREIYAPLANRLGINRFKWELEDLAFKFLEPDEYKNLKD
QIVVKRSDREKRLDNTLNLMKENLVSSGLKNFEITGRPKHLYGIWSKMERQQKQFSEIYDVAALRIIVSNLDSCYKALAV
VHDTFRPIPGRFKDYIGLPKPNGYQSLHTSVIGKHRPIEVQIRTTSMHQIAEFGIAAHWQYKEGGSPASSNAERFNWLRQ
LVEWQQEGNEKDHNDYLASIKEDLFDEEVFVITPKGDVVGLRKGSTAIDFAYRIHSEIGNHCNGIRINEKLSPLSTSLQN
GDFIEILTNNNSTPSLDWLNFVVTPTAKNRIRQWYKKSHRDETIKRGKDLLEKEIGRNGFESLLSSDAMQKVAHRCNLKT
TEDLLASLGFGGLTLHQVLNRLREEIKIQTEEIKNESNAEIAKTLVNKNNSITTKSHATNKSPISGVEGLDYRIGKCCSP
LPGEEIIGTVSLGNHGITIHRRDCENVLPIPIERRLPVAWNQENKVLDNKFPIQLRIEVIDRVGVLKDILMRLSDKGINV
SDANVKTAFGKPAIINLCVGLESYSQLHKTIDQIKSMADVLDIARVGIS

Sequences:

>Translated_769_residues
MSEATADSKERKEIEVSTIIVPENKNYESESLKYEIKIPNWLLENIQNYELSNNKNDSNQDLIVKAFKLAYQAHNGQLRA
SGEPYIIHPIAVADLLKEIGASSSVIAAGLLHDVVEDTGIALSEIEINFGLEVKVLVEGVTKLGGIHFNNRTEAQAENLR
KMFLAMASDIRVVLVKLADRLHNMRTIQWLNEERKERIARETREIYAPLANRLGINRFKWELEDLAFKFLEPDEYKNLKD
QIVVKRSDREKRLDNTLNLMKENLVSSGLKNFEITGRPKHLYGIWSKMERQQKQFSEIYDVAALRIIVSNLDSCYKALAV
VHDTFRPIPGRFKDYIGLPKPNGYQSLHTSVIGKHRPIEVQIRTTSMHQIAEFGIAAHWQYKEGGSPASSNAERFNWLRQ
LVEWQQEGNEKDHNDYLASIKEDLFDEEVFVITPKGDVVGLRKGSTAIDFAYRIHSEIGNHCNGIRINEKLSPLSTSLQN
GDFIEILTNNNSTPSLDWLNFVVTPTAKNRIRQWYKKSHRDETIKRGKDLLEKEIGRNGFESLLSSDAMQKVAHRCNLKT
TEDLLASLGFGGLTLHQVLNRLREEIKIQTEEIKNESNAEIAKTLVNKNNSITTKSHATNKSPISGVEGLDYRIGKCCSP
LPGEEIIGTVSLGNHGITIHRRDCENVLPIPIERRLPVAWNQENKVLDNKFPIQLRIEVIDRVGVLKDILMRLSDKGINV
SDANVKTAFGKPAIINLCVGLESYSQLHKTIDQIKSMADVLDIARVGIS
>Mature_768_residues
SEATADSKERKEIEVSTIIVPENKNYESESLKYEIKIPNWLLENIQNYELSNNKNDSNQDLIVKAFKLAYQAHNGQLRAS
GEPYIIHPIAVADLLKEIGASSSVIAAGLLHDVVEDTGIALSEIEINFGLEVKVLVEGVTKLGGIHFNNRTEAQAENLRK
MFLAMASDIRVVLVKLADRLHNMRTIQWLNEERKERIARETREIYAPLANRLGINRFKWELEDLAFKFLEPDEYKNLKDQ
IVVKRSDREKRLDNTLNLMKENLVSSGLKNFEITGRPKHLYGIWSKMERQQKQFSEIYDVAALRIIVSNLDSCYKALAVV
HDTFRPIPGRFKDYIGLPKPNGYQSLHTSVIGKHRPIEVQIRTTSMHQIAEFGIAAHWQYKEGGSPASSNAERFNWLRQL
VEWQQEGNEKDHNDYLASIKEDLFDEEVFVITPKGDVVGLRKGSTAIDFAYRIHSEIGNHCNGIRINEKLSPLSTSLQNG
DFIEILTNNNSTPSLDWLNFVVTPTAKNRIRQWYKKSHRDETIKRGKDLLEKEIGRNGFESLLSSDAMQKVAHRCNLKTT
EDLLASLGFGGLTLHQVLNRLREEIKIQTEEIKNESNAEIAKTLVNKNNSITTKSHATNKSPISGVEGLDYRIGKCCSPL
PGEEIIGTVSLGNHGITIHRRDCENVLPIPIERRLPVAWNQENKVLDNKFPIQLRIEVIDRVGVLKDILMRLSDKGINVS
DANVKTAFGKPAIINLCVGLESYSQLHKTIDQIKSMADVLDIARVGIS

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=137, Percent_Identity=39.4160583941606, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1790082, Length=707, Percent_Identity=38.4724186704385, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI1789147, Length=698, Percent_Identity=35.1002865329513, Blast_Score=449, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 87132; Mature: 87000

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEATADSKERKEIEVSTIIVPENKNYESESLKYEIKIPNWLLENIQNYELSNNKNDSNQ
CCCCCCCCHHHCEEEEEEEEEECCCCCCCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCH
DLIVKAFKLAYQAHNGQLRASGEPYIIHPIAVADLLKEIGASSSVIAAGLLHDVVEDTGI
HHHHHHHHHHHHHCCCEEEECCCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
ALSEIEINFGLEVKVLVEGVTKLGGIHFNNRTEAQAENLRKMFLAMASDIRVVLVKLADR
EEEEEEEECCCEEHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHNMRTIQWLNEERKERIARETREIYAPLANRLGINRFKWELEDLAFKFLEPDEYKNLKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHCCCHH
QIVVKRSDREKRLDNTLNLMKENLVSSGLKNFEITGRPKHLYGIWSKMERQQKQFSEIYD
HEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
VAALRIIVSNLDSCYKALAVVHDTFRPIPGRFKDYIGLPKPNGYQSLHTSVIGKHRPIEV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCEEE
QIRTTSMHQIAEFGIAAHWQYKEGGSPASSNAERFNWLRQLVEWQQEGNEKDHNDYLASI
EEECCHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KEDLFDEEVFVITPKGDVVGLRKGSTAIDFAYRIHSEIGNHCNGIRINEKLSPLSTSLQN
HHHHCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHCCC
GDFIEILTNNNSTPSLDWLNFVVTPTAKNRIRQWYKKSHRDETIKRGKDLLEKEIGRNGF
CCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHH
ESLLSSDAMQKVAHRCNLKTTEDLLASLGFGGLTLHQVLNRLREEIKIQTEEIKNESNAE
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHEHHHHHCCCCCHH
IAKTLVNKNNSITTKSHATNKSPISGVEGLDYRIGKCCSPLPGEEIIGTVSLGNHGITIH
HHHHHHCCCCCEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHEEEEEECCCCEEEE
RRDCENVLPIPIERRLPVAWNQENKVLDNKFPIQLRIEVIDRVGVLKDILMRLSDKGINV
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCC
SDANVKTAFGKPAIINLCVGLESYSQLHKTIDQIKSMADVLDIARVGIS
CCCCCEECCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEATADSKERKEIEVSTIIVPENKNYESESLKYEIKIPNWLLENIQNYELSNNKNDSNQ
CCCCCCCHHHCEEEEEEEEEECCCCCCCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCH
DLIVKAFKLAYQAHNGQLRASGEPYIIHPIAVADLLKEIGASSSVIAAGLLHDVVEDTGI
HHHHHHHHHHHHHCCCEEEECCCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
ALSEIEINFGLEVKVLVEGVTKLGGIHFNNRTEAQAENLRKMFLAMASDIRVVLVKLADR
EEEEEEEECCCEEHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHNMRTIQWLNEERKERIARETREIYAPLANRLGINRFKWELEDLAFKFLEPDEYKNLKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHCCCHH
QIVVKRSDREKRLDNTLNLMKENLVSSGLKNFEITGRPKHLYGIWSKMERQQKQFSEIYD
HEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
VAALRIIVSNLDSCYKALAVVHDTFRPIPGRFKDYIGLPKPNGYQSLHTSVIGKHRPIEV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCEEE
QIRTTSMHQIAEFGIAAHWQYKEGGSPASSNAERFNWLRQLVEWQQEGNEKDHNDYLASI
EEECCHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KEDLFDEEVFVITPKGDVVGLRKGSTAIDFAYRIHSEIGNHCNGIRINEKLSPLSTSLQN
HHHHCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHCCC
GDFIEILTNNNSTPSLDWLNFVVTPTAKNRIRQWYKKSHRDETIKRGKDLLEKEIGRNGF
CCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHH
ESLLSSDAMQKVAHRCNLKTTEDLLASLGFGGLTLHQVLNRLREEIKIQTEEIKNESNAE
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHEHHHHHCCCCCHH
IAKTLVNKNNSITTKSHATNKSPISGVEGLDYRIGKCCSPLPGEEIIGTVSLGNHGITIH
HHHHHHCCCCCEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHEEEEEECCCCEEEE
RRDCENVLPIPIERRLPVAWNQENKVLDNKFPIQLRIEVIDRVGVLKDILMRLSDKGINV
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCC
SDANVKTAFGKPAIINLCVGLESYSQLHKTIDQIKSMADVLDIARVGIS
CCCCCEECCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]