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The map label for this gene is mesJ [H]

Identifier: 123969392

GI number: 123969392

Start: 1592893

End: 1593903

Strand: Direct

Name: mesJ [H]

Synonym: A9601_18601

Alternate gene names: 123969392

Gene position: 1592893-1593903 (Clockwise)

Preceding gene: 123969389

Following gene: 123969396

Centisome position: 95.39

GC content: 28.19

Gene sequence:

>1011_bases
ATGACTGATAAAAAATTAAGTCAGAAAAATTGGTCATCATGGCATCATCAGCTTCATAAGGAGATTCTTGCCAAAAAAAT
ATTAATTCCCAAAGGATCCAATATTTTAATAAGTGTTTCAGGGGGTCAAGACTCAATGGCCTTATTAACCCTAATTAATG
ACCTAAAAAAACTACATAATTGGTCTATTAGTGTTTGGCATGGTGATCATCAGTGGCACGAAAAATCATCACTATATGCT
CTTGAATTAAAAGATTATTGCGAAGATAAAAATATTTCATTCTCTTTTGATCAAGCAAATAAAGAAGATATTTCTTCAGA
AGAAAAAGCACGAGAATGGAGATATAAAAAATTATGTGAAAGAGCCAAAACTTTATTAAGTACAAACCAGGAAAAACATA
ATATTTATTTGCTGACTGGTCACACGAGTAGTGATAATGCAGAAACATTTATTCTCAATTTATCTAGAGGAAGCAATTTT
GCAGGTCTGAGTAATATTGAGAGTAAAAGATTAATAGAAAATCAAATTTATTTAATAAGACCAATATTAATTTTCAGTAG
GGAAGATACAAAACAATTTTGCAATGAAATGAAGATTCCTGTCTGGGAAGATCCTACAAATTCAGATCTTAAATTAAAAA
GAAATTTAGTAAGGAAAAAAATTATTCCTATCTTGGAAGTCATCTATCCTGGTTGTTCTGAAAGGATAAATAATTTTTCC
CAAAAAATGAGCAAATACAATAATGAACGTAATGATCTAAGTGAACTTGCGTATTTTTATTGTAAAGATATTAAAGGTAT
CGATAGGAATCTCCTAAATGGTATGTGTATTGAAGCGAGATGCACAATTTTAAATAGATTTTTAAAAGAAATATCTCCAA
AGCAATGTAGTTCTAAAAATCTGACAAAATTAGCAACTTCAATTTATGAAAAAAATAAAGGTCAAATTAATCTGCAAGAG
TTTTTAAAAATTGTTTGGGATAAAAACTATATAAATTTTGAAAAAAGTTAA

Upstream 100 bases:

>100_bases
ATTAATCAGACTCATTGACTTAATGAATTTATTACCCGATTGTGACATTGTTTTAGTAAAACAGTGCATTTTTTAAAAAA
TATTATCTGAGCAACACAAA

Downstream 100 bases:

>100_bases
GATGTAACAGCAGATCTTCTTCTTCTTGTTCTACCTTCAAATGAATCTTCTGTAGCAGTCTCAGCTGATGGATTCTGTGA
TACTTTTGGACTTTTTTGGG

Product: ATPase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTDKKLSQKNWSSWHHQLHKEILAKKILIPKGSNILISVSGGQDSMALLTLINDLKKLHNWSISVWHGDHQWHEKSSLYA
LELKDYCEDKNISFSFDQANKEDISSEEKAREWRYKKLCERAKTLLSTNQEKHNIYLLTGHTSSDNAETFILNLSRGSNF
AGLSNIESKRLIENQIYLIRPILIFSREDTKQFCNEMKIPVWEDPTNSDLKLKRNLVRKKIIPILEVIYPGCSERINNFS
QKMSKYNNERNDLSELAYFYCKDIKGIDRNLLNGMCIEARCTILNRFLKEISPKQCSSKNLTKLATSIYEKNKGQINLQE
FLKIVWDKNYINFEKS

Sequences:

>Translated_336_residues
MTDKKLSQKNWSSWHHQLHKEILAKKILIPKGSNILISVSGGQDSMALLTLINDLKKLHNWSISVWHGDHQWHEKSSLYA
LELKDYCEDKNISFSFDQANKEDISSEEKAREWRYKKLCERAKTLLSTNQEKHNIYLLTGHTSSDNAETFILNLSRGSNF
AGLSNIESKRLIENQIYLIRPILIFSREDTKQFCNEMKIPVWEDPTNSDLKLKRNLVRKKIIPILEVIYPGCSERINNFS
QKMSKYNNERNDLSELAYFYCKDIKGIDRNLLNGMCIEARCTILNRFLKEISPKQCSSKNLTKLATSIYEKNKGQINLQE
FLKIVWDKNYINFEKS
>Mature_335_residues
TDKKLSQKNWSSWHHQLHKEILAKKILIPKGSNILISVSGGQDSMALLTLINDLKKLHNWSISVWHGDHQWHEKSSLYAL
ELKDYCEDKNISFSFDQANKEDISSEEKAREWRYKKLCERAKTLLSTNQEKHNIYLLTGHTSSDNAETFILNLSRGSNFA
GLSNIESKRLIENQIYLIRPILIFSREDTKQFCNEMKIPVWEDPTNSDLKLKRNLVRKKIIPILEVIYPGCSERINNFSQ
KMSKYNNERNDLSELAYFYCKDIKGIDRNLLNGMCIEARCTILNRFLKEISPKQCSSKNLTKLATSIYEKNKGQINLQEF
LKIVWDKNYINFEKS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=275, Percent_Identity=24.3636363636364, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 39303; Mature: 39171

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKKLSQKNWSSWHHQLHKEILAKKILIPKGSNILISVSGGQDSMALLTLINDLKKLHN
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHC
WSISVWHGDHQWHEKSSLYALELKDYCEDKNISFSFDQANKEDISSEEKAREWRYKKLCE
CEEEEEECCCHHCCCCCEEEEEEHHHCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHH
RAKTLLSTNQEKHNIYLLTGHTSSDNAETFILNLSRGSNFAGLSNIESKRLIENQIYLIR
HHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHCCHHEEE
PILIFSREDTKQFCNEMKIPVWEDPTNSDLKLKRNLVRKKIIPILEVIYPGCSERINNFS
HEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QKMSKYNNERNDLSELAYFYCKDIKGIDRNLLNGMCIEARCTILNRFLKEISPKQCSSKN
HHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHCCEEHHHHHHHHHHHHHHCCCCCCCCCH
LTKLATSIYEKNKGQINLQEFLKIVWDKNYINFEKS
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TDKKLSQKNWSSWHHQLHKEILAKKILIPKGSNILISVSGGQDSMALLTLINDLKKLHN
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHC
WSISVWHGDHQWHEKSSLYALELKDYCEDKNISFSFDQANKEDISSEEKAREWRYKKLCE
CEEEEEECCCHHCCCCCEEEEEEHHHCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHH
RAKTLLSTNQEKHNIYLLTGHTSSDNAETFILNLSRGSNFAGLSNIESKRLIENQIYLIR
HHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHCCHHEEE
PILIFSREDTKQFCNEMKIPVWEDPTNSDLKLKRNLVRKKIIPILEVIYPGCSERINNFS
HEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QKMSKYNNERNDLSELAYFYCKDIKGIDRNLLNGMCIEARCTILNRFLKEISPKQCSSKN
HHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHCCEEHHHHHHHHHHHHHHCCCCCCCCCH
LTKLATSIYEKNKGQINLQEFLKIVWDKNYINFEKS
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]