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The map label for this gene is hemK [C]

Identifier: 123967879

GI number: 123967879

Start: 313413

End: 314282

Strand: Direct

Name: hemK [C]

Synonym: A9601_03421

Alternate gene names: 123967879

Gene position: 313413-314282 (Clockwise)

Preceding gene: 123967878

Following gene: 123967880

Centisome position: 18.77

GC content: 30.0

Gene sequence:

>870_bases
ATGCTTTGCATTTCTGTAAAGGAATTTTTATTGTGGAAAAAAAAGCAACTTTCAAAAGGTGGTGATCAACAATCTTTTGA
AGTTTTACTTGATTGTTTAGGCGATATTTCGACTAGAGATCTAAATTTGAAAATTTTAAATCCTAAGGGAAACTTACATT
TAAAAAAAAACTTAGAATTTTTGGAGTCTGTTTGGGAAGATCATTTAATAAGATCTTGCCCAATTCAATACCTTTGCGGA
TTAACTTATTGGAGAGACTTAAAATTAAAAGTTACAAATAAAGTACTTATTCCCAGACCAGAGACAGAGCTCATAGTAGA
TATTGTCTTCAATATATTTAGAAGGAAGTCAGAGAAATTATTTTTTGCTGAATTAGGGACTGGATCAGGCGCTATAAGTA
TTGCGTTAGCATTGGCTTATCCATTTAGCAAAGGAGTGGCAACTGATGTAGATCAAGACGCATTAGAAATAGCTACAAAA
AATTATATAAATTCTTCCAAACAATCAAATTTGAAATTTTATTGTGGAAATTGGTGGTCACCTCTTGAAAATTTTAAAGG
AAAATTAGACCTTGCTATTTCAAACCCGCCATATATTCCTAAAGATACTTATGAAAAATTACCCAAAGAAGTTAAAAATT
TCGAACCTAAAGTTGCTTTATTAGGCGGTGAAGATGGTTTAAAACACATTAGGGAAATAATACAAAAAGCACCAATATTT
TTAAAAGAGAAGGGCTGGCTAATTTTAGAGAATCATTTTGATCAAGGCGAAAAAGTAAAACAACTTTTTATTAAAAATAA
ATTTACATCAATAGAAATTGTGAAAGATCTTTCAGGTATTGGTAGGTTTACCATTGGAAGATATAAATAA

Upstream 100 bases:

>100_bases
TCATTGGTTTATTTATCTCTACTCAAGATGGAGAAAAGTCAAGCTTCTACTCTGACTCTAGTAAAGGTAGACTTGGACCA
AAACGCTGATGCTTTAATAA

Downstream 100 bases:

>100_bases
ATTATTTCAAGTTCATAATCTAAATGGATTTAGTAGACTGCAAATCCGCTTTGAAGACTCTTAAAAGTGGTTTACCTATA
ATCTTCCCAACTGACACATT

Product: putative protein methyltransferase

Products: Protoporphyrin IX; H2O

Alternate protein names: M.Ssp6803HemKP [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MLCISVKEFLLWKKKQLSKGGDQQSFEVLLDCLGDISTRDLNLKILNPKGNLHLKKNLEFLESVWEDHLIRSCPIQYLCG
LTYWRDLKLKVTNKVLIPRPETELIVDIVFNIFRRKSEKLFFAELGTGSGAISIALALAYPFSKGVATDVDQDALEIATK
NYINSSKQSNLKFYCGNWWSPLENFKGKLDLAISNPPYIPKDTYEKLPKEVKNFEPKVALLGGEDGLKHIREIIQKAPIF
LKEKGWLILENHFDQGEKVKQLFIKNKFTSIEIVKDLSGIGRFTIGRYK

Sequences:

>Translated_289_residues
MLCISVKEFLLWKKKQLSKGGDQQSFEVLLDCLGDISTRDLNLKILNPKGNLHLKKNLEFLESVWEDHLIRSCPIQYLCG
LTYWRDLKLKVTNKVLIPRPETELIVDIVFNIFRRKSEKLFFAELGTGSGAISIALALAYPFSKGVATDVDQDALEIATK
NYINSSKQSNLKFYCGNWWSPLENFKGKLDLAISNPPYIPKDTYEKLPKEVKNFEPKVALLGGEDGLKHIREIIQKAPIF
LKEKGWLILENHFDQGEKVKQLFIKNKFTSIEIVKDLSGIGRFTIGRYK
>Mature_289_residues
MLCISVKEFLLWKKKQLSKGGDQQSFEVLLDCLGDISTRDLNLKILNPKGNLHLKKNLEFLESVWEDHLIRSCPIQYLCG
LTYWRDLKLKVTNKVLIPRPETELIVDIVFNIFRRKSEKLFFAELGTGSGAISIALALAYPFSKGVATDVDQDALEIATK
NYINSSKQSNLKFYCGNWWSPLENFKGKLDLAISNPPYIPKDTYEKLPKEVKNFEPKVALLGGEDGLKHIREIIQKAPIF
LKEKGWLILENHFDQGEKVKQLFIKNKFTSIEIVKDLSGIGRFTIGRYK

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=228, Percent_Identity=30.2631578947368, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1787463, Length=215, Percent_Identity=33.4883720930233, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI87082085, Length=178, Percent_Identity=32.0224719101124, Blast_Score=86, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24582226, Length=198, Percent_Identity=31.3131313131313, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 1.3.3.4

Molecular weight: Translated: 33110; Mature: 33110

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCISVKEFLLWKKKQLSKGGDQQSFEVLLDCLGDISTRDLNLKILNPKGNLHLKKNLEF
CEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEHHCHHH
LESVWEDHLIRSCPIQYLCGLTYWRDLKLKVTNKVLIPRPETELIVDIVFNIFRRKSEKL
HHHHHHHHHHHHCCHHHHHHHHHHHEEEEEEECEEECCCCCHHHHHHHHHHHHHCCCCEE
FFAELGTGSGAISIALALAYPFSKGVATDVDQDALEIATKNYINSSKQSNLKFYCGNWWS
EEEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCC
PLENFKGKLDLAISNPPYIPKDTYEKLPKEVKNFEPKVALLGGEDGLKHIREIIQKAPIF
HHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCEE
LKEKGWLILENHFDQGEKVKQLFIKNKFTSIEIVKDLSGIGRFTIGRYK
EECCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLCISVKEFLLWKKKQLSKGGDQQSFEVLLDCLGDISTRDLNLKILNPKGNLHLKKNLEF
CEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEHHCHHH
LESVWEDHLIRSCPIQYLCGLTYWRDLKLKVTNKVLIPRPETELIVDIVFNIFRRKSEKL
HHHHHHHHHHHHCCHHHHHHHHHHHEEEEEEECEEECCCCCHHHHHHHHHHHHHCCCCEE
FFAELGTGSGAISIALALAYPFSKGVATDVDQDALEIATKNYINSSKQSNLKFYCGNWWS
EEEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCC
PLENFKGKLDLAISNPPYIPKDTYEKLPKEVKNFEPKVALLGGEDGLKHIREIIQKAPIF
HHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCEE
LKEKGWLILENHFDQGEKVKQLFIKNKFTSIEIVKDLSGIGRFTIGRYK
EECCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protoporphyrinogen IX; O2

Specific reaction: protoporphyrinogen-IX + 1.5 O2 = protoporphyrin-IX + 3 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]