Click here to switch to the map view.

The map label for this gene is ntcA [H]

Identifier: 123967805

GI number: 123967805

Start: 248828

End: 249562

Strand: Direct

Name: ntcA [H]

Synonym: A9601_02681

Alternate gene names: 123967805

Gene position: 248828-249562 (Clockwise)

Preceding gene: 123967801

Following gene: 123967806

Centisome position: 14.9

GC content: 36.6

Gene sequence:

>735_bases
ATGATACCTGCTTCACGAGGATTCAATCGCTTTAGTTCGCAACAGTCAGGACAAAGTAAAATAAATTCTGTTGGAGAACG
TTTTCCAATTAATAGAACTTTAATGGAAGTTATTAAAGGTCTAGATGGTGCAAGCACAGAAATGATTGAGAGATCGAAAA
CAATATTCTTCCCTGGTGACCCTGCTGAAAGGGTTTATCTAATAAGAAGAGGAGCAGTAAGACTGTCAAGAGTTTATGAG
TCAGGTGAAGAAATAACTGTTGCTCTTTTAAGAGAGAATAGTCTCTTTGGTGTTTTATCTCTTCTAACAGGCCATAGATC
TGATCGTTTCTATCATGCAATAGCATTCACAAGAGTAGAAATGATAACGGCACCGGCAAATTCGGTTTTAAGAGCTATAG
AGGAAGATGCATCAGTCGGACTATTACTTTTACAGGGGCTATCAAGTAGGATACTGCAAACTGAAACAATGATAGAAACT
CTTACACATAGAGATATGTCTTCTCGTTTAGTAAGTTTTTTAATGGTTCTTTGTAGAGACTTTGGAGTTGCAGGTGAAAA
AGGGATTACAATAGACTTAAGGCTTTCACATCAGGCAATTGCTGAAGCTATTGGTTCGACAAGGGTAACCATTACAAGAT
TATTGGGAGATTTAAAGGATTCAGGGCTTCTTAATATTGAAAGAAAAAAGATCACAGTTTTCGATCCAATTGCTCTTGCA
AAAAGATTTAATTGA

Upstream 100 bases:

>100_bases
AAAGAGGCTTATTGATAATCTAATAAGAATTGAAAACTGTTACTGTTGATACAAGATATTTTAGAGTGTCTTCTTAAATT
TTTTAATAGTCAAGATATTT

Downstream 100 bases:

>100_bases
TTCATCATAAATTATGATGTACTGAGTTTATGCAAAAGTGTGGCTTGGATTTTAATTGTTTTTTTAATATTACTCGGGGG
ATTAATTGCACCATTTGGGG

Product: CRP family global nitrogen regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MIPASRGFNRFSSQQSGQSKINSVGERFPINRTLMEVIKGLDGASTEMIERSKTIFFPGDPAERVYLIRRGAVRLSRVYE
SGEEITVALLRENSLFGVLSLLTGHRSDRFYHAIAFTRVEMITAPANSVLRAIEEDASVGLLLLQGLSSRILQTETMIET
LTHRDMSSRLVSFLMVLCRDFGVAGEKGITIDLRLSHQAIAEAIGSTRVTITRLLGDLKDSGLLNIERKKITVFDPIALA
KRFN

Sequences:

>Translated_244_residues
MIPASRGFNRFSSQQSGQSKINSVGERFPINRTLMEVIKGLDGASTEMIERSKTIFFPGDPAERVYLIRRGAVRLSRVYE
SGEEITVALLRENSLFGVLSLLTGHRSDRFYHAIAFTRVEMITAPANSVLRAIEEDASVGLLLLQGLSSRILQTETMIET
LTHRDMSSRLVSFLMVLCRDFGVAGEKGITIDLRLSHQAIAEAIGSTRVTITRLLGDLKDSGLLNIERKKITVFDPIALA
KRFN
>Mature_244_residues
MIPASRGFNRFSSQQSGQSKINSVGERFPINRTLMEVIKGLDGASTEMIERSKTIFFPGDPAERVYLIRRGAVRLSRVYE
SGEEITVALLRENSLFGVLSLLTGHRSDRFYHAIAFTRVEMITAPANSVLRAIEEDASVGLLLLQGLSSRILQTETMIET
LTHRDMSSRLVSFLMVLCRDFGVAGEKGITIDLRLSHQAIAEAIGSTRVTITRLLGDLKDSGLLNIERKKITVFDPIALA
KRFN

Specific function: Required for full expression of proteins subject to ammonium repression. Transcriptional activator of genes subject to nitrogen control [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=193, Percent_Identity=26.9430051813472, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 27115; Mature: 27115

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPASRGFNRFSSQQSGQSKINSVGERFPINRTLMEVIKGLDGASTEMIERSKTIFFPGD
CCCCCCCCHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHCCCEEEECCC
PAERVYLIRRGAVRLSRVYESGEEITVALLRENSLFGVLSLLTGHRSDRFYHAIAFTRVE
CHHHEEEHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHH
MITAPANSVLRAIEEDASVGLLLLQGLSSRILQTETMIETLTHRDMSSRLVSFLMVLCRD
HHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FGVAGEKGITIDLRLSHQAIAEAIGSTRVTITRLLGDLKDSGLLNIERKKITVFDPIALA
CCCCCCCCCEEEEEECHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCEEEEECHHHHH
KRFN
HCCC
>Mature Secondary Structure
MIPASRGFNRFSSQQSGQSKINSVGERFPINRTLMEVIKGLDGASTEMIERSKTIFFPGD
CCCCCCCCHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHCCCEEEECCC
PAERVYLIRRGAVRLSRVYESGEEITVALLRENSLFGVLSLLTGHRSDRFYHAIAFTRVE
CHHHEEEHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHH
MITAPANSVLRAIEEDASVGLLLLQGLSSRILQTETMIETLTHRDMSSRLVSFLMVLCRD
HHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FGVAGEKGITIDLRLSHQAIAEAIGSTRVTITRLLGDLKDSGLLNIERKKITVFDPIALA
CCCCCCCCCEEEEEECHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCEEEEECHHHHH
KRFN
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8366058; 8905231 [H]