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The map label for this gene is queA [H]

Identifier: 121998492

GI number: 121998492

Start: 1874079

End: 1875116

Strand: Reverse

Name: queA [H]

Synonym: Hhal_1713

Alternate gene names: 121998492

Gene position: 1875116-1874079 (Counterclockwise)

Preceding gene: 121998493

Following gene: 121998488

Centisome position: 70.01

GC content: 70.33

Gene sequence:

>1038_bases
GTGACCGAGTGGCGCCGCAGTGACTTTGACTACGCCTTGCCCGAGCGGCTGATCGCCCATCGGCCGGCGGCGCAACGCGC
CGCTAGCCGGATGCTGGTGCTGCGCACCGATAGCGGGGCGCTGGCCGATCGGCGCTTCACCGACCTGCCGGCCTATCTGC
GCGCCGGGGACCTGCTGGTCCTCAACGACACACGTGTGATCCCGGCCCGGCTCGAGGCGGCCAAGCCCACCGGGGCGCGG
GTCGAGGTTTTGATCGAGCGGGTGGAAGATCCGGACGCGGGGCTGATCACTTGTCAGCTGCGTGCCAACCGGACCCCGAA
GCCCGGCGGCCGGCTGGGGTTGGCCGACGGGGTGCAGGCCGAGGTGCTCGAGCGCGAGGGCCGCTTCTTCCGTTTGCAGC
TGGCTGGGCTGGTCGAAAGCCTGTGGGACTACCTGGAGCGGGTCGGCCACGTGCCGTTGCCGCCGTATATCCGCCGGCCG
GACGACGACGAGGATCGCACCCGCTACCAGACCGTTTTCGCCGCCCGCCCGGGGGCCGTCGCCGCGCCGACGGCGGGGCT
GCACTTCGATGACGCCCTGCTCGAGCGGTTACGCGCCGACGGGGTGGGGACGGCTCGGGTGACGCTGCACGTGGGTGCCG
GGACGTTCGCCCCGGTGGAGAGCGATGATCTGAGTGCCCACACCATGCACGCCGAGTGGCTGGAGGTCAGCGCCGAGACG
GTGGCCGCTGTCAACGCTACCCGAGAGCGCGGCGGCCGGGTGGTGGCCGTGGGGACTACGGTGGTGCGGGCCCTGGAGAG
TGCCGCGGCGGACGGCCAGCTGGCACCTTACAGTGGCGAGACGCGGCTCTTCATCACTCCGGGGTACTGTTTCCGGGCCG
TGGACGGACTGCTCACCAACTTCCACCTGCCCGAGTCGACGCTGCTGATGCTCGTCAGCGCCTTCGGCGGCTACGAATCG
GTCATGGCCGCCTACCGTCACGCCGTGGCCGAAGAGTACCGCTTCTTCAGCTACGGCGATGCCATGCTGCTGATCTGA

Upstream 100 bases:

>100_bases
TCAACCAGGCCGCGCAGACCGCCCAGCAGCTGCGGCGTGAGGCCGAGGCCCTGCAGGAGTTGGTGCAGCAGTTCAAGGTG
ACAGAGGGTGGCCCGTCATC

Downstream 100 bases:

>100_bases
TCCGTCTGGGCACGGGTGCGGCTAGTCTTCGGCGCGTTCGAGCTGCCGGTGACAGCGGGCGCAGCGGTAGATCTGCGTGC
CGCGCAGGATGCGGTTGTGA

Product: S-adenosylmethionine--tRNA-ribosyltransferase- isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTEWRRSDFDYALPERLIAHRPAAQRAASRMLVLRTDSGALADRRFTDLPAYLRAGDLLVLNDTRVIPARLEAAKPTGAR
VEVLIERVEDPDAGLITCQLRANRTPKPGGRLGLADGVQAEVLEREGRFFRLQLAGLVESLWDYLERVGHVPLPPYIRRP
DDDEDRTRYQTVFAARPGAVAAPTAGLHFDDALLERLRADGVGTARVTLHVGAGTFAPVESDDLSAHTMHAEWLEVSAET
VAAVNATRERGGRVVAVGTTVVRALESAAADGQLAPYSGETRLFITPGYCFRAVDGLLTNFHLPESTLLMLVSAFGGYES
VMAAYRHAVAEEYRFFSYGDAMLLI

Sequences:

>Translated_345_residues
MTEWRRSDFDYALPERLIAHRPAAQRAASRMLVLRTDSGALADRRFTDLPAYLRAGDLLVLNDTRVIPARLEAAKPTGAR
VEVLIERVEDPDAGLITCQLRANRTPKPGGRLGLADGVQAEVLEREGRFFRLQLAGLVESLWDYLERVGHVPLPPYIRRP
DDDEDRTRYQTVFAARPGAVAAPTAGLHFDDALLERLRADGVGTARVTLHVGAGTFAPVESDDLSAHTMHAEWLEVSAET
VAAVNATRERGGRVVAVGTTVVRALESAAADGQLAPYSGETRLFITPGYCFRAVDGLLTNFHLPESTLLMLVSAFGGYES
VMAAYRHAVAEEYRFFSYGDAMLLI
>Mature_344_residues
TEWRRSDFDYALPERLIAHRPAAQRAASRMLVLRTDSGALADRRFTDLPAYLRAGDLLVLNDTRVIPARLEAAKPTGARV
EVLIERVEDPDAGLITCQLRANRTPKPGGRLGLADGVQAEVLEREGRFFRLQLAGLVESLWDYLERVGHVPLPPYIRRPD
DDEDRTRYQTVFAARPGAVAAPTAGLHFDDALLERLRADGVGTARVTLHVGAGTFAPVESDDLSAHTMHAEWLEVSAETV
AAVNATRERGGRVVAVGTTVVRALESAAADGQLAPYSGETRLFITPGYCFRAVDGLLTNFHLPESTLLMLVSAFGGYESV
MAAYRHAVAEEYRFFSYGDAMLLI

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=53.4682080924855, Blast_Score=359, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 37816; Mature: 37685

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEWRRSDFDYALPERLIAHRPAAQRAASRMLVLRTDSGALADRRFTDLPAYLRAGDLLV
CCCCCCCCCCHHHHHHHHHCCCHHHHHHCEEEEEECCCCCCCCCCHHCCCHHHCCCCEEE
LNDTRVIPARLEAAKPTGARVEVLIERVEDPDAGLITCQLRANRTPKPGGRLGLADGVQA
EECCEECHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHH
EVLEREGRFFRLQLAGLVESLWDYLERVGHVPLPPYIRRPDDDEDRTRYQTVFAARPGAV
HHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHEEEEECCCCC
AAPTAGLHFDDALLERLRADGVGTARVTLHVGAGTFAPVESDDLSAHTMHAEWLEVSAET
CCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHEEHHHHHHHHHHH
VAAVNATRERGGRVVAVGTTVVRALESAAADGQLAPYSGETRLFITPGYCFRAVDGLLTN
HHHHHHHHHHCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHH
FHLPESTLLMLVSAFGGYESVMAAYRHAVAEEYRFFSYGDAMLLI
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCEEEEC
>Mature Secondary Structure 
TEWRRSDFDYALPERLIAHRPAAQRAASRMLVLRTDSGALADRRFTDLPAYLRAGDLLV
CCCCCCCCCHHHHHHHHHCCCHHHHHHCEEEEEECCCCCCCCCCHHCCCHHHCCCCEEE
LNDTRVIPARLEAAKPTGARVEVLIERVEDPDAGLITCQLRANRTPKPGGRLGLADGVQA
EECCEECHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHH
EVLEREGRFFRLQLAGLVESLWDYLERVGHVPLPPYIRRPDDDEDRTRYQTVFAARPGAV
HHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHEEEEECCCCC
AAPTAGLHFDDALLERLRADGVGTARVTLHVGAGTFAPVESDDLSAHTMHAEWLEVSAET
CCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHEEHHHHHHHHHHH
VAAVNATRERGGRVVAVGTTVVRALESAAADGQLAPYSGETRLFITPGYCFRAVDGLLTN
HHHHHHHHHHCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHH
FHLPESTLLMLVSAFGGYESVMAAYRHAVAEEYRFFSYGDAMLLI
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA