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The map label for this gene is opuD [H]

Identifier: 121998166

GI number: 121998166

Start: 1491265

End: 1492839

Strand: Reverse

Name: opuD [H]

Synonym: Hhal_1384

Alternate gene names: 121998166

Gene position: 1492839-1491265 (Counterclockwise)

Preceding gene: 121998167

Following gene: 121998165

Centisome position: 55.74

GC content: 65.27

Gene sequence:

>1575_bases
GTGCGTGCACAGAAAGGCCCGCTGAAAGGGCTGAATATTCCGTTGACGGGCACGGCCACGCTGATCGTGCTCGCTTTCCT
CATCTTCGGTGCCTGGGATCCGGAATACGCCGAGACGGTCTTCGAGGGCATCAGCGGCTGGGTCATCGAGACCTTCAAGT
GGTACTACATCGGGGTCGTCGCGTTCTTCCTGCTCTTCGCGCTGTTCCTGATGTTCAGCCGCTTCGGTGACCTCAAGCTT
GGGGACGACGACCGGCCACCGGAGTTCAGTTACTTCGCCTGGTTCTCCATGCTCTTCGGTGCCGGTATGGGCATCGGCCT
GTTGTTCTGGAGCATCGCCGAGCCGGTCTGGCACTTCCAGGGCAACCCCTTCATCGACGAGGGTGAGACGGCCGCCGCGG
CTGACTCGGCCATGCGCCTGACCTACTTCCACTGGGGTATGCACCCGTGGGCCATCTACGCCATCGTGGCGCTTTCGCTG
GCCTTCTTCTGCTACCGCAAGAAGCTGCCGCTGGCCATCCGTTCGGCGCTCTATCCGCTCATCGGCAACCGCATCTACGG
CCCGATCGGCCACGCGGCGGACGTCCTGGCGGTCTTCGGCACCATCTTCGGCGTGGCCACCTCCCTGGGGTTCGGTGCAA
TCCAGATCAACACCGGCCTCAACGAACTCACCGGGCTTGAGCTCAGTGTCACCAACCAGCTGCTGATCGTCGCCGTGGTC
ACCCTGATTGCCGTGGGTTCGGTGATCTCCGGCGTGGGGCGGGGCGTGAAGGTCCTCTCGCAGCTGAACCTGATCCTCAG
CGCCGTGATCCTGCTCTTCTTCCTGAGCTTCGGGCCGACCCTTTATCTGCTCTCGAGCTTCGTCCAGGGCATCGGCGACT
ACCTGCAGAACGTGGTCTACCTCAGCTTCTGGACCGACGCCAGCGGGGCCCGTGAGGCCGGCGACTGGCAGCTCTCGTGG
ACGGCCTTCTATTGGGGCTGGTGGATCGCCTGGGCCCCCTTCGTGGGTATGTTCATCGCCCGCATCTCCCGCGGTCGCAC
CATCCGCGAGTTTCTGGGCGGCGTGCTGCTGGTGCCGACCCTGCTCGCCCTGGGCTGGCTGACCGTCTTCGGTGGCACCG
GCCTGTACCAGGAGCTCTTCGGTGCCGGGGGGCTGGTCGAGGCGGTGTCCGAGGATGAGACCATCGCCCTTTACTACACC
ATCGAAGCGGTGGCCCCCGGGGTGATTGCCACCATCTTTGCGGCGATCGCCACGGTCCTCATCGCCACCTACTTCATCAC
CTCGTCGGACTCGGCGACGCTGGTGGTGACCATGCTGCTGTCTGTCGGCAACACCGAGCCGCCGACCTACCAGCGGGCCT
TCTGGGGCGTGGCCGAAGGCTGCGTGGCCGCGGTACTGCTGGTGGCTGGTGGCTTGGTTGCCCTGCAGGCGGCGGCCATC
GTCGCGGCGTTGCCGTTCTCGCTGCTGATGCTGCTGATGTGCTACGCCCTGATCCGCGGGCTGCAGGAGGAGAAGCGGCG
TATGCAGCTCTCCTGGCAGCCGGGGCAGGGGCCGCCGGCGGCACCGCATCTGTGA

Upstream 100 bases:

>100_bases
ACCGCGAAAGTTCCCGCGGTGATGTGGTCTACCGGGCCGTGGGTGGCTACGGCGATCACGTCGGCGAAGAGTACTTCTAG
AGCACCAGGGGGGGCGAATC

Downstream 100 bases:

>100_bases
GCGGTTGTGAGCGGTGACCCGGACCCCCGTCGCGCGCCCTGGGAGGCGCGCTGGCGGGAAGGGCGCACCGGCTGGGACCG
GGGCGGGGTAAGCCCCACCC

Product: choline/carnitine/betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MRAQKGPLKGLNIPLTGTATLIVLAFLIFGAWDPEYAETVFEGISGWVIETFKWYYIGVVAFFLLFALFLMFSRFGDLKL
GDDDRPPEFSYFAWFSMLFGAGMGIGLLFWSIAEPVWHFQGNPFIDEGETAAAADSAMRLTYFHWGMHPWAIYAIVALSL
AFFCYRKKLPLAIRSALYPLIGNRIYGPIGHAADVLAVFGTIFGVATSLGFGAIQINTGLNELTGLELSVTNQLLIVAVV
TLIAVGSVISGVGRGVKVLSQLNLILSAVILLFFLSFGPTLYLLSSFVQGIGDYLQNVVYLSFWTDASGAREAGDWQLSW
TAFYWGWWIAWAPFVGMFIARISRGRTIREFLGGVLLVPTLLALGWLTVFGGTGLYQELFGAGGLVEAVSEDETIALYYT
IEAVAPGVIATIFAAIATVLIATYFITSSDSATLVVTMLLSVGNTEPPTYQRAFWGVAEGCVAAVLLVAGGLVALQAAAI
VAALPFSLLMLLMCYALIRGLQEEKRRMQLSWQPGQGPPAAPHL

Sequences:

>Translated_524_residues
MRAQKGPLKGLNIPLTGTATLIVLAFLIFGAWDPEYAETVFEGISGWVIETFKWYYIGVVAFFLLFALFLMFSRFGDLKL
GDDDRPPEFSYFAWFSMLFGAGMGIGLLFWSIAEPVWHFQGNPFIDEGETAAAADSAMRLTYFHWGMHPWAIYAIVALSL
AFFCYRKKLPLAIRSALYPLIGNRIYGPIGHAADVLAVFGTIFGVATSLGFGAIQINTGLNELTGLELSVTNQLLIVAVV
TLIAVGSVISGVGRGVKVLSQLNLILSAVILLFFLSFGPTLYLLSSFVQGIGDYLQNVVYLSFWTDASGAREAGDWQLSW
TAFYWGWWIAWAPFVGMFIARISRGRTIREFLGGVLLVPTLLALGWLTVFGGTGLYQELFGAGGLVEAVSEDETIALYYT
IEAVAPGVIATIFAAIATVLIATYFITSSDSATLVVTMLLSVGNTEPPTYQRAFWGVAEGCVAAVLLVAGGLVALQAAAI
VAALPFSLLMLLMCYALIRGLQEEKRRMQLSWQPGQGPPAAPHL
>Mature_524_residues
MRAQKGPLKGLNIPLTGTATLIVLAFLIFGAWDPEYAETVFEGISGWVIETFKWYYIGVVAFFLLFALFLMFSRFGDLKL
GDDDRPPEFSYFAWFSMLFGAGMGIGLLFWSIAEPVWHFQGNPFIDEGETAAAADSAMRLTYFHWGMHPWAIYAIVALSL
AFFCYRKKLPLAIRSALYPLIGNRIYGPIGHAADVLAVFGTIFGVATSLGFGAIQINTGLNELTGLELSVTNQLLIVAVV
TLIAVGSVISGVGRGVKVLSQLNLILSAVILLFFLSFGPTLYLLSSFVQGIGDYLQNVVYLSFWTDASGAREAGDWQLSW
TAFYWGWWIAWAPFVGMFIARISRGRTIREFLGGVLLVPTLLALGWLTVFGGTGLYQELFGAGGLVEAVSEDETIALYYT
IEAVAPGVIATIFAAIATVLIATYFITSSDSATLVVTMLLSVGNTEPPTYQRAFWGVAEGCVAAVLLVAGGLVALQAAAI
VAALPFSLLMLLMCYALIRGLQEEKRRMQLSWQPGQGPPAAPHL

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=466, Percent_Identity=40.5579399141631, Blast_Score=354, Evalue=8e-99,
Organism=Escherichia coli, GI1786224, Length=483, Percent_Identity=26.9151138716356, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1788102, Length=441, Percent_Identity=26.7573696145125, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 56890; Mature: 56890

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAQKGPLKGLNIPLTGTATLIVLAFLIFGAWDPEYAETVFEGISGWVIETFKWYYIGVV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
AFFLLFALFLMFSRFGDLKLGDDDRPPEFSYFAWFSMLFGAGMGIGLLFWSIAEPVWHFQ
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
GNPFIDEGETAAAADSAMRLTYFHWGMHPWAIYAIVALSLAFFCYRKKLPLAIRSALYPL
CCCCCCCCCCHHHHHHHHEEHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IGNRIYGPIGHAADVLAVFGTIFGVATSLGFGAIQINTGLNELTGLELSVTNQLLIVAVV
HCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCEEHHHHHHHHHHHH
TLIAVGSVISGVGRGVKVLSQLNLILSAVILLFFLSFGPTLYLLSSFVQGIGDYLQNVVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHEE
LSFWTDASGAREAGDWQLSWTAFYWGWWIAWAPFVGMFIARISRGRTIREFLGGVLLVPT
EEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHH
LLALGWLTVFGGTGLYQELFGAGGLVEAVSEDETIALYYTIEAVAPGVIATIFAAIATVL
HHHHHHHHHHCCCHHHHHHHCCCCHHHHCCCCCEEEEEEEHHHHCCHHHHHHHHHHHHHH
IATYFITSSDSATLVVTMLLSVGNTEPPTYQRAFWGVAEGCVAAVLLVAGGLVALQAAAI
HHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAALPFSLLMLLMCYALIRGLQEEKRRMQLSWQPGQGPPAAPHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]