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The map label for this gene is glnG [H]

Identifier: 121998005

GI number: 121998005

Start: 1318758

End: 1320173

Strand: Reverse

Name: glnG [H]

Synonym: Hhal_1215

Alternate gene names: 121998005

Gene position: 1320173-1318758 (Counterclockwise)

Preceding gene: 121998006

Following gene: 121998001

Centisome position: 49.29

GC content: 68.15

Gene sequence:

>1416_bases
ATGACTGAGGCCGAATCGCAGGTATGGGTGGTGGACGATGACCGGTCCATCCGCTGGGTGCTGGAGAAGGCGCTGCGCCG
CTCCGGCCACGGTGTCCGCACCTTCGAGAGCGGCGATGCGGCGCTGGAGGCGCTGCGCCGGGAAGAGCCCGATGTACTGA
TCACCGATCTGCGCATGCCGGGGCTGGGCGGTCACGATCTGCTCCAGCAAGTCCAGGCGTCCACGCCGCAACTGCCGGTG
ATCGTGATCACCGCCTATTCGGATCTCGATGCGGCGGTTAGCTCCTACGAGGGCGGCGCCTTCGAGTACCTCCCCAAGCC
CTTCGATGTGGACGAGGCGGTGGATCTGGCGGGCCGGGCCATCGCCAGTCGGCGCAAGGGCGCCGCGGCGGAAGCCGCGG
GGGGAGGCTCCGCCGATATCCTCGGTGAGGCGCCGGCCATGCAGGAGGTGTTTCGCGCCATCGGTCGGTTGTCGCGTTCC
AGCGTCACGGTGTTGCTCACCGGCGAGTCCGGTAGCGGCAAGGAGTTGGTGGCCCGCGCGCTGCACCGCCACAGCCCGCG
TTCCGACGGTCCCTTCGTCGCTCTGAACATGGCCGCCATCCCTCACGACCTGATGGAATCAGAGCTTTTCGGGCACGAAA
AGGGGGCCTTCACCGGGGCGCAGCAGACTCGCCGCGGGCGTTTCGAGCAGGCCGACGGTGGTACGCTCTTCCTCGACGAG
ATCGGCGATATGCCCGCCGACCTGCAGACCCGCTTACTGCGGGTGCTCGCCGACGGTGAGTTCTATCGGGTGGGTGCCCA
TACCGCCATGAAGGTGGATGTACGCATTATCGCTGCCACCCACCAGGATCTGGAGCAGCGGGTGGTCGAGGGGCGTTTCC
GCGAGGATCTCTACCACCGCCTGAACGTGATCCGCATCCAGCTGCCGCCACTGCGTGAGCGGCGTGAGGATATCCCGGCC
CTGGCCCGCCGTTTCCTGGCGGCCGCCGCCCAGGAGCTCAACGTCGACCCGAAGGCGCTGCATCCCGATGTGGAGCGCGA
ACTGATGCGGCTCGATTGGCCCGGCAATGTGCGTCAGCTGGAGAATACCTGCCGCTGGCTGACGGTGATGGCCAGCGGTC
AGGAGATCGTCCCCGAAGATCTGCCAGCCGACCTGCGCCGAGGCAGTCCCGGCGGCAGCCCGGGTGGCACGGACAGCGGC
AGCGGCGACTGGGAGGCGGTCTTGGCGCGGTGGGCCGAACAGGCCCTCGACGGTGGCGAGGATCACCTGCTCGACGACGC
CTTGCCACGCTTCGAACGGGTCCTGATTCGCGCCGCGCTGAGCCGCAGCGGCGGCCACCGCCAGGAGGCCGCGCGGTTAC
TCGGCTGGGGTCGGAATACGCTGACGCGCAAGATCAAGGAGCTGGGCATGGACTGA

Upstream 100 bases:

>100_bases
ATTGCGCAGAGCCTGGTCAGCCGCCTCGGTGGCCTGATTGAGTGCGTCAGCGAACCGGGGCGCACGGTGTTCACCATCTG
GTTACCCATGGAGACGGAAA

Downstream 100 bases:

>100_bases
GCCGCCGACGGTTCGCTTCCCCGCGGTCAGTCGTCGAATTCGGCCTTCGGTGTCCGCTCCATGAGCTCGCGGACCGCCTG
CTCGAGTGGGGTGTCGCCGC

Product: nitrogen metabolism transcriptional regulator NtrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTEAESQVWVVDDDRSIRWVLEKALRRSGHGVRTFESGDAALEALRREEPDVLITDLRMPGLGGHDLLQQVQASTPQLPV
IVITAYSDLDAAVSSYEGGAFEYLPKPFDVDEAVDLAGRAIASRRKGAAAEAAGGGSADILGEAPAMQEVFRAIGRLSRS
SVTVLLTGESGSGKELVARALHRHSPRSDGPFVALNMAAIPHDLMESELFGHEKGAFTGAQQTRRGRFEQADGGTLFLDE
IGDMPADLQTRLLRVLADGEFYRVGAHTAMKVDVRIIAATHQDLEQRVVEGRFREDLYHRLNVIRIQLPPLRERREDIPA
LARRFLAAAAQELNVDPKALHPDVERELMRLDWPGNVRQLENTCRWLTVMASGQEIVPEDLPADLRRGSPGGSPGGTDSG
SGDWEAVLARWAEQALDGGEDHLLDDALPRFERVLIRAALSRSGGHRQEAARLLGWGRNTLTRKIKELGMD

Sequences:

>Translated_471_residues
MTEAESQVWVVDDDRSIRWVLEKALRRSGHGVRTFESGDAALEALRREEPDVLITDLRMPGLGGHDLLQQVQASTPQLPV
IVITAYSDLDAAVSSYEGGAFEYLPKPFDVDEAVDLAGRAIASRRKGAAAEAAGGGSADILGEAPAMQEVFRAIGRLSRS
SVTVLLTGESGSGKELVARALHRHSPRSDGPFVALNMAAIPHDLMESELFGHEKGAFTGAQQTRRGRFEQADGGTLFLDE
IGDMPADLQTRLLRVLADGEFYRVGAHTAMKVDVRIIAATHQDLEQRVVEGRFREDLYHRLNVIRIQLPPLRERREDIPA
LARRFLAAAAQELNVDPKALHPDVERELMRLDWPGNVRQLENTCRWLTVMASGQEIVPEDLPADLRRGSPGGSPGGTDSG
SGDWEAVLARWAEQALDGGEDHLLDDALPRFERVLIRAALSRSGGHRQEAARLLGWGRNTLTRKIKELGMD
>Mature_470_residues
TEAESQVWVVDDDRSIRWVLEKALRRSGHGVRTFESGDAALEALRREEPDVLITDLRMPGLGGHDLLQQVQASTPQLPVI
VITAYSDLDAAVSSYEGGAFEYLPKPFDVDEAVDLAGRAIASRRKGAAAEAAGGGSADILGEAPAMQEVFRAIGRLSRSS
VTVLLTGESGSGKELVARALHRHSPRSDGPFVALNMAAIPHDLMESELFGHEKGAFTGAQQTRRGRFEQADGGTLFLDEI
GDMPADLQTRLLRVLADGEFYRVGAHTAMKVDVRIIAATHQDLEQRVVEGRFREDLYHRLNVIRIQLPPLRERREDIPAL
ARRFLAAAAQELNVDPKALHPDVERELMRLDWPGNVRQLENTCRWLTVMASGQEIVPEDLPADLRRGSPGGSPGGTDSGS
GDWEAVLARWAEQALDGGEDHLLDDALPRFERVLIRAALSRSGGHRQEAARLLGWGRNTLTRKIKELGMD

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=466, Percent_Identity=66.0944206008584, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI1788550, Length=474, Percent_Identity=40.7172995780591, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI1790437, Length=466, Percent_Identity=42.274678111588, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1788905, Length=480, Percent_Identity=37.0833333333333, Blast_Score=295, Evalue=5e-81,
Organism=Escherichia coli, GI87082117, Length=241, Percent_Identity=51.4522821576763, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1789233, Length=335, Percent_Identity=45.3731343283582, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1789087, Length=335, Percent_Identity=41.1940298507463, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1786524, Length=341, Percent_Identity=41.3489736070381, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1787583, Length=335, Percent_Identity=38.8059701492537, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI87082152, Length=335, Percent_Identity=39.4029850746269, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI87081872, Length=226, Percent_Identity=47.3451327433628, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI87081858, Length=316, Percent_Identity=33.5443037974684, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1789828, Length=279, Percent_Identity=34.4086021505376, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI1790860, Length=107, Percent_Identity=36.4485981308411, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789809, Length=152, Percent_Identity=28.9473684210526, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51677; Mature: 51546

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAESQVWVVDDDRSIRWVLEKALRRSGHGVRTFESGDAALEALRREEPDVLITDLRMP
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCCEEEEECCCC
GLGGHDLLQQVQASTPQLPVIVITAYSDLDAAVSSYEGGAFEYLPKPFDVDEAVDLAGRA
CCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHH
IASRRKGAAAEAAGGGSADILGEAPAMQEVFRAIGRLSRSSVTVLLTGESGSGKELVARA
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
LHRHSPRSDGPFVALNMAAIPHDLMESELFGHEKGAFTGAQQTRRGRFEQADGGTLFLDE
HHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEHHH
IGDMPADLQTRLLRVLADGEFYRVGAHTAMKVDVRIIAATHQDLEQRVVEGRFREDLYHR
HCCCCHHHHHHHHHHHHCCCEEEECCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHH
LNVIRIQLPPLRERREDIPALARRFLAAAAQELNVDPKALHPDVERELMRLDWPGNVRQL
HCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHCCCCCCHHHH
ENTCRWLTVMASGQEIVPEDLPADLRRGSPGGSPGGTDSGSGDWEAVLARWAEQALDGGE
HHHHHEEEEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
DHLLDDALPRFERVLIRAALSRSGGHRQEAARLLGWGRNTLTRKIKELGMD
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEAESQVWVVDDDRSIRWVLEKALRRSGHGVRTFESGDAALEALRREEPDVLITDLRMP
CCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCCEEEEECCCC
GLGGHDLLQQVQASTPQLPVIVITAYSDLDAAVSSYEGGAFEYLPKPFDVDEAVDLAGRA
CCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHH
IASRRKGAAAEAAGGGSADILGEAPAMQEVFRAIGRLSRSSVTVLLTGESGSGKELVARA
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
LHRHSPRSDGPFVALNMAAIPHDLMESELFGHEKGAFTGAQQTRRGRFEQADGGTLFLDE
HHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEHHH
IGDMPADLQTRLLRVLADGEFYRVGAHTAMKVDVRIIAATHQDLEQRVVEGRFREDLYHR
HCCCCHHHHHHHHHHHHCCCEEEECCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHH
LNVIRIQLPPLRERREDIPALARRFLAAAAQELNVDPKALHPDVERELMRLDWPGNVRQL
HCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHCCCCCCHHHH
ENTCRWLTVMASGQEIVPEDLPADLRRGSPGGSPGGTDSGSGDWEAVLARWAEQALDGGE
HHHHHEEEEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
DHLLDDALPRFERVLIRAALSRSGGHRQEAARLLGWGRNTLTRKIKELGMD
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]