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The map label for this gene is ntrB [H]

Identifier: 121998006

GI number: 121998006

Start: 1320166

End: 1321239

Strand: Reverse

Name: ntrB [H]

Synonym: Hhal_1216

Alternate gene names: 121998006

Gene position: 1321239-1320166 (Counterclockwise)

Preceding gene: 121998007

Following gene: 121998005

Centisome position: 49.33

GC content: 67.69

Gene sequence:

>1074_bases
ATGGAAGATCACGAGCGCGACCCATCGCAGATCCTCGAGGAGCTGACGACCGCCGTCCTGCTGGTGGATGACCGGGTGCG
TATCCTCCACGTCAACCACGCCGCCGAGACGCTGTTCCGGGTCAGTAGCCGACAGGTGGTGGGCCAGACCCTGGGGACGG
CACTGCGCGGGGCGGAACTGCTCGAAGAGCTCATCCGCCAGACCCAGCGTACCGGCGGGGCCTACACCCAGCGCGAACGT
CGCCTTCCGGTCCGCGGCGATCGCCCGGTGACCGTCGACTGCACCATCACCCCGGTCTCCGCCAAACGGGTGCTGATCGA
GATCGCCGAGGTGGACCGTCACGCCCGTATTACCCGCGAACAGCACCTGCTGTCACAGAATCGTGCCGTACAGGAGCTGA
TCCGCGGACTGGCCCACGAGATCAAGAACCCGCTCGGCGGCCTGCGCGGCGCGGCGCAGTTGCTGGAGGCCGAGCTCCCG
GAGCGCGACCAGCGCGAGTACACCCAGGTCATTATCCGCGAGGCGGACCGCCTGCAGCAGCTGGTGGACGCCCTGCTCGG
TCCCAATGCGCCGGCCCGTGAGGAGCCGGTCAACATCCATGAGGTCCTCGAGCGCGTCCGTTCGCTGGTCATCGCCGAGG
ATGCCGAAGGCCGCGCAGAGGCGCCAGCGGTGGCGCTCCAGCGCGACTACGATCCGAGCATCCCGCCGGTCACCGCCGAG
CACAATCACCTGGTCCAGGCGGTGCTCAACCTGGTGCGCAACGCCCGTCAGGCCACCGGGCCAGGGGGGACCATCACCCT
GCGTACGCGCACCCAGCGTCAGTTCACCATCGCCGACAAGCCCCACCGCCTGGTGGCCCGCATCGACATCATCGACGACG
GGCCGGGGATCCCCCTCGATCAGCAGGAGCAGATCTTCTATCCGATGGTCACCTCGCGCCCCGAGGGTACCGGTTTGGGA
CTGCCCATTGCGCAGAGCCTGGTCAGCCGCCTCGGTGGCCTGATTGAGTGCGTCAGCGAACCGGGGCGCACGGTGTTCAC
CATCTGGTTACCCATGGAGACGGAAAATGACTGA

Upstream 100 bases:

>100_bases
GCATTGCACCCGGATGGTGCAGTCACCGTACGCCTGCGCGTGCATCCGACCGCGCCCATCGGGGGTGTCGGGGTGGCCCG
GGCTTTGCACAGGGATGGAC

Downstream 100 bases:

>100_bases
GGCCGAATCGCAGGTATGGGTGGTGGACGATGACCGGTCCATCCGCTGGGTGCTGGAGAAGGCGCTGCGCCGCTCCGGCC
ACGGTGTCCGCACCTTCGAG

Product: signal transduction histidine kinase NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MEDHERDPSQILEELTTAVLLVDDRVRILHVNHAAETLFRVSSRQVVGQTLGTALRGAELLEELIRQTQRTGGAYTQRER
RLPVRGDRPVTVDCTITPVSAKRVLIEIAEVDRHARITREQHLLSQNRAVQELIRGLAHEIKNPLGGLRGAAQLLEAELP
ERDQREYTQVIIREADRLQQLVDALLGPNAPAREEPVNIHEVLERVRSLVIAEDAEGRAEAPAVALQRDYDPSIPPVTAE
HNHLVQAVLNLVRNARQATGPGGTITLRTRTQRQFTIADKPHRLVARIDIIDDGPGIPLDQQEQIFYPMVTSRPEGTGLG
LPIAQSLVSRLGGLIECVSEPGRTVFTIWLPMETEND

Sequences:

>Translated_357_residues
MEDHERDPSQILEELTTAVLLVDDRVRILHVNHAAETLFRVSSRQVVGQTLGTALRGAELLEELIRQTQRTGGAYTQRER
RLPVRGDRPVTVDCTITPVSAKRVLIEIAEVDRHARITREQHLLSQNRAVQELIRGLAHEIKNPLGGLRGAAQLLEAELP
ERDQREYTQVIIREADRLQQLVDALLGPNAPAREEPVNIHEVLERVRSLVIAEDAEGRAEAPAVALQRDYDPSIPPVTAE
HNHLVQAVLNLVRNARQATGPGGTITLRTRTQRQFTIADKPHRLVARIDIIDDGPGIPLDQQEQIFYPMVTSRPEGTGLG
LPIAQSLVSRLGGLIECVSEPGRTVFTIWLPMETEND
>Mature_357_residues
MEDHERDPSQILEELTTAVLLVDDRVRILHVNHAAETLFRVSSRQVVGQTLGTALRGAELLEELIRQTQRTGGAYTQRER
RLPVRGDRPVTVDCTITPVSAKRVLIEIAEVDRHARITREQHLLSQNRAVQELIRGLAHEIKNPLGGLRGAAQLLEAELP
ERDQREYTQVIIREADRLQQLVDALLGPNAPAREEPVNIHEVLERVRSLVIAEDAEGRAEAPAVALQRDYDPSIPPVTAE
HNHLVQAVLNLVRNARQATGPGGTITLRTRTQRQFTIADKPHRLVARIDIIDDGPGIPLDQQEQIFYPMVTSRPEGTGLG
LPIAQSLVSRLGGLIECVSEPGRTVFTIWLPMETEND

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=347, Percent_Identity=45.5331412103746, Blast_Score=270, Evalue=9e-74,
Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=32.7510917030568, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1788549, Length=355, Percent_Identity=26.1971830985915, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI87082128, Length=235, Percent_Identity=28.5106382978723, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87081816, Length=238, Percent_Identity=27.7310924369748, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994928, Length=300, Percent_Identity=24.3333333333333, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790346, Length=233, Percent_Identity=27.8969957081545, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790567, Length=356, Percent_Identity=23.5955056179775, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 39782; Mature: 39782

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDHERDPSQILEELTTAVLLVDDRVRILHVNHAAETLFRVSSRQVVGQTLGTALRGAEL
CCCCCCCHHHHHHHHHHHEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEELIRQTQRTGGAYTQRERRLPVRGDRPVTVDCTITPVSAKRVLIEIAEVDRHARITRE
HHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
QHLLSQNRAVQELIRGLAHEIKNPLGGLRGAAQLLEAELPERDQREYTQVIIREADRLQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LVDALLGPNAPAREEPVNIHEVLERVRSLVIAEDAEGRAEAPAVALQRDYDPSIPPVTAE
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHEECCCCCCCCCCCCC
HNHLVQAVLNLVRNARQATGPGGTITLRTRTQRQFTIADKPHRLVARIDIIDDGPGIPLD
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEECCCCCEEEEEEEEEECCCCCCCC
QQEQIFYPMVTSRPEGTGLGLPIAQSLVSRLGGLIECVSEPGRTVFTIWLPMETEND
CCCCEEECHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MEDHERDPSQILEELTTAVLLVDDRVRILHVNHAAETLFRVSSRQVVGQTLGTALRGAEL
CCCCCCCHHHHHHHHHHHEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEELIRQTQRTGGAYTQRERRLPVRGDRPVTVDCTITPVSAKRVLIEIAEVDRHARITRE
HHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
QHLLSQNRAVQELIRGLAHEIKNPLGGLRGAAQLLEAELPERDQREYTQVIIREADRLQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LVDALLGPNAPAREEPVNIHEVLERVRSLVIAEDAEGRAEAPAVALQRDYDPSIPPVTAE
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHEECCCCCCCCCCCCC
HNHLVQAVLNLVRNARQATGPGGTITLRTRTQRQFTIADKPHRLVARIDIIDDGPGIPLD
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEECCCCCEEEEEEEEEECCCCCCCC
QQEQIFYPMVTSRPEGTGLGLPIAQSLVSRLGGLIECVSEPGRTVFTIWLPMETEND
CCCCEEECHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]