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The map label for this gene is hisF

Identifier: 121997882

GI number: 121997882

Start: 1184968

End: 1185732

Strand: Reverse

Name: hisF

Synonym: Hhal_1091

Alternate gene names: 121997882

Gene position: 1185732-1184968 (Counterclockwise)

Preceding gene: 121997883

Following gene: 121997881

Centisome position: 44.27

GC content: 69.28

Gene sequence:

>765_bases
ATGACCGCTAAGCGCATCATCCCGTGTTTGGACGTCGACGCCGGCCGAGTGGTCAAGGGGGTGCGCTTCGTGGATATTCG
CGATGCCGGCGACCCGGTGGAGATCGCCCGGCGCTACGACGCCATGGGGGCCGACGAACTGACGTTTCTCGACATCACGG
CCAGCCACGAGGAGCGTGAGACCATCTACGATGTAGTGGAGGCCGTGGCCGGCCAGGTCTTCATCCCGCTGACCGTAGGC
GGCGGCGTGCGCAGCGTGGCCGATGTACGTCGCCTGCTCAATGCCGGTGCCGACAAGGTGGCCATCAACACCGCGGCCGT
CCATCGCCCCGACTTCGTCCGCGAGGCTGCCGAGCGCTTCGGCTCCCAGTGCATCGTGGTGGCGGTGGACGCCAAGCAGG
TGGAGGACGATCCGCCGCGTTGGGAGGTCTTCACCCACGGCGGGCGTAACCCCACTGGGCTCGAGGTGGTCAGCTGGTGT
CAGCGCCTGGTGGCGCTGGGGGCCGGCGAGATCCTGCTGACCAGCATGGACCGGGACGGCACCCGCGCCGGGTTCGACCT
GGGGCTGACGCGGGCCGTGGCCGATGCGGTCTCCGTCCCGGTGATCGCATCGGGCGGTGTGGGCGAGCTGGAGCACCTGG
CTGACGGGGTCGACGACGGGGGCGCCGACGCGGTGCTGGCGGCGAGCATCTTCCATTTCGGCGATCACACCGTCGGCGAG
GCCAAGGCGCACATGGCGGAACGGGGGATCGAGGTCCGACGATGA

Upstream 100 bases:

>100_bases
GGTAGCCCCGAAGGCATCGCCGGGGCGATCGTGGGCCGCGCCATCTATGACGGTGGCCTCGATCTGGCCACCGCGATCCG
CACCGCGCAGGAGGTCGGCG

Downstream 100 bases:

>100_bases
GCAATGCCGCGAGCGAGCGAACGGCAGCCGACACCCTGCGGGCGCTGGCCGAGACCATCGAACAGCGCCGCGGCGCGTCG
GCGGATACCTCCTACGTGGC

Product: imidazoleglycerol phosphate synthase, cyclase subunit

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTAKRIIPCLDVDAGRVVKGVRFVDIRDAGDPVEIARRYDAMGADELTFLDITASHEERETIYDVVEAVAGQVFIPLTVG
GGVRSVADVRRLLNAGADKVAINTAAVHRPDFVREAAERFGSQCIVVAVDAKQVEDDPPRWEVFTHGGRNPTGLEVVSWC
QRLVALGAGEILLTSMDRDGTRAGFDLGLTRAVADAVSVPVIASGGVGELEHLADGVDDGGADAVLAASIFHFGDHTVGE
AKAHMAERGIEVRR

Sequences:

>Translated_254_residues
MTAKRIIPCLDVDAGRVVKGVRFVDIRDAGDPVEIARRYDAMGADELTFLDITASHEERETIYDVVEAVAGQVFIPLTVG
GGVRSVADVRRLLNAGADKVAINTAAVHRPDFVREAAERFGSQCIVVAVDAKQVEDDPPRWEVFTHGGRNPTGLEVVSWC
QRLVALGAGEILLTSMDRDGTRAGFDLGLTRAVADAVSVPVIASGGVGELEHLADGVDDGGADAVLAASIFHFGDHTVGE
AKAHMAERGIEVRR
>Mature_253_residues
TAKRIIPCLDVDAGRVVKGVRFVDIRDAGDPVEIARRYDAMGADELTFLDITASHEERETIYDVVEAVAGQVFIPLTVGG
GVRSVADVRRLLNAGADKVAINTAAVHRPDFVREAAERFGSQCIVVAVDAKQVEDDPPRWEVFTHGGRNPTGLEVVSWCQ
RLVALGAGEILLTSMDRDGTRAGFDLGLTRAVADAVSVPVIASGGVGELEHLADGVDDGGADAVLAASIFHFGDHTVGEA
KAHMAERGIEVRR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=43.1906614785992, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI87082028, Length=242, Percent_Identity=31.8181818181818, Blast_Score=97, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6319725, Length=312, Percent_Identity=34.9358974358974, Blast_Score=158, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_HALHL (A1WW05)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002669.1
- ProteinModelPortal:   A1WW05
- SMR:   A1WW05
- STRING:   A1WW05
- GeneID:   4709917
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1091
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   A1WW05
- BioCyc:   HHAL349124:HHAL_1091-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27021; Mature: 26890

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKRIIPCLDVDAGRVVKGVRFVDIRDAGDPVEIARRYDAMGADELTFLDITASHEERE
CCCHHCCCCCCCCCHHHHCCEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEECCCHHHH
TIYDVVEAVAGQVFIPLTVGGGVRSVADVRRLLNAGADKVAINTAAVHRPDFVREAAERF
HHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHHH
GSQCIVVAVDAKQVEDDPPRWEVFTHGGRNPTGLEVVSWCQRLVALGAGEILLTSMDRDG
CCCEEEEEEEHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCC
TRAGFDLGLTRAVADAVSVPVIASGGVGELEHLADGVDDGGADAVLAASIFHFGDHTVGE
CCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHH
AKAHMAERGIEVRR
HHHHHHHCCCCCCC
>Mature Secondary Structure 
TAKRIIPCLDVDAGRVVKGVRFVDIRDAGDPVEIARRYDAMGADELTFLDITASHEERE
CCHHCCCCCCCCCHHHHCCEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEECCCHHHH
TIYDVVEAVAGQVFIPLTVGGGVRSVADVRRLLNAGADKVAINTAAVHRPDFVREAAERF
HHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHHH
GSQCIVVAVDAKQVEDDPPRWEVFTHGGRNPTGLEVVSWCQRLVALGAGEILLTSMDRDG
CCCEEEEEEEHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCC
TRAGFDLGLTRAVADAVSVPVIASGGVGELEHLADGVDDGGADAVLAASIFHFGDHTVGE
CCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHH
AKAHMAERGIEVRR
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA