Click here to switch to the map view.

The map label for this gene is spoT [H]

Identifier: 121997760

GI number: 121997760

Start: 1040683

End: 1042872

Strand: Reverse

Name: spoT [H]

Synonym: Hhal_0969

Alternate gene names: 121997760

Gene position: 1042872-1040683 (Counterclockwise)

Preceding gene: 121997761

Following gene: 121997759

Centisome position: 38.94

GC content: 68.26

Gene sequence:

>2190_bases
ATGGCGCGCGCCGCGAGTGTCATCAACGCCGGTGAATCGGCGTCGGCTTCCGACCCGGAGCGGCGCGTTCAGGAGGTTTG
TGCGCTACTCGAGCGCTACCTCGAGCCCCACCAGATCGAGCAGGTCTACGACGCCTACCGTTTCAGCGCCGCCGCCCACG
AGGGGCAGCGGCGCCGCTCCGGCGAGCCCTACATCACCCACCCGATCGCCGTGGCCCGCATCCTCGCCGAGATGCGCCTG
GATGCCGAGGCCGTTGTAGCCGCGCTGCTCCACGACGTCATCGAGGATACGCCCACCGCCAAGGAGCGGATCGCCGAGCG
CTTCGGCGAGCAGGTGGCCGAGCTGGTGGACGGAGTCTCCAAGCTCGGCGCCATCGACTTCCAGAGCAAGGAAGAGGCCC
AGGCGGAGAGCTTCCGCAAGATGATCCTGGCCATGGCCAAGGACATCCGCGTCATCCTCATCAAGCTCGCCGACCGCCTC
CACAACATGCGCACGATCTGGATCATGCGTCCGGAGAAGCGCCGCCGCATCGCCCGCGAGACCCTCGACATCTACGCCCC
CATCGCCCAGCGGCTGGGCATCAACAGCCTGCGCACCGAGCTCGAGGACCTGGGCTTTGCCGCGCACTACCCGCTGCGCT
ACCGGGCGCTCAAGGAGAAGCTCGCCCGCCAGCGGGGGCGGCGCGGGGTGATCCTCGACACCGTCTGCGACCAGGTGCGC
ACCCGCCTGGAGCAGGCTGGGATCCAGGCCGAGGTGGTCGGGCGTGAGAAGCATCTGTGGTCCATCTACTGCAAGATGGC
CCAGAAACAGCTCAACTTCCGCGACGTCTTCGACGTCTACGGCTTCCGGGTGGTGGTGGACACGGTGGACGAGTGCTACC
GCGTCCTCGGCATCCTGCACGGGCTCTACAAGCCGCGCCCGGGGCGCATCAAGGACTACATCGCCATCCCCAAGGCCAAC
GGCTACCAGTCGCTGCACACCACGCTGGTCGGGCCGCATCGCATCCGCCTCGAGGCGCAGATCCGCACCCGGGATATGCA
CGCCGTGGCCGAGTCGGGCATCGCCGCCCACTGGTTCTATAAGGATGCCGGCGAGAGCGGTAACACGGCCCAGGTCCGTG
CCCGGCAGTGGGTCAAGGACCTGCTTGAGATGCAGCGCAGTGTCGGCACGTCCGAGGAGTTCATCGAGAGCGTCAAGATC
GACCTGGTCCCCGACGAGATCTACGTCTTCACCCCCAAGGGCGACATCCTCGAGCTGCCGAGCAATGCCACCCCGGTGGA
TCTGGCCTACGCCATCCACTCTGACGTGGGTAACTCCTGCATCGCCGCCAAGGTCGACCACCGCCTCACGCCACTGCGTA
CGCCGCTGCAGAGCGGGCAGATGGTCGAGATCATCACCGCCCCGGTGGGGCGGCCCAATCCGGTCTGGCTGGATTTCGTG
GTGACCGCCAAGGCGCGCACCGCCATCCGTCACGCCCTCAAGCGGCTGCGCGACGACGAGGCCATCGAGCTCGGCCGGCG
CATGGTCGAGCGCGCCCTCTCGGCGTTGTCGGTGGATCTCGACGCCCTCGACCCGGCGCGCGTCGACGAGGCCCTGGCCG
GCACGGGCCTCGCCAGTCTCGACGACCTGTTCCGCGATGTCGGTCTGGGCAACCGGGTGCCGTGGCTGGTGGCGCAGCAG
CTCAGTGGTCTCGAGCAGGGCGAGGCCGAGCTGGAAGAGCGCCCCGAGGAGCCCGAGCCCCACGGGACCCTGACCATCCG
CGGCAACGAGGGCACGGTGGTCTCCTTCGCGCGCTGCTGCCGGCCGATCCCCGGCGACCCGATCGTCGGCTTCGTCAGCA
CCGGTCGTGGCGTGGTCATCCACCACCGTGATTGCAAGAACGTGGCCAGTCAGCGCAAGCGTTCCGAGAGCTGGGTGGAC
GTGGACTGGGAGGCCGACGTCGATGCCGAGTTCCCCACCCTGATCGGCATCGACGTGGTCAACGAGCGTGGCGCCCTGGC
TCAGCTCGCCCACGGGATCTCGGCGCAAGGGGCGAGCATCGAGAACGTGACCATCGAGGAGCGCGACGGCATGATCGCCA
CCGTCCGCTTCCTGATCCTGGTTCGTGACCGCCGCCACCTGGCGGCCGTGGTGCGCCATCTGCGGGCGATGCCCCCGGTG
CAGCGCATCGTGCGCAAGAAAGACGGCTAA

Upstream 100 bases:

>100_bases
GCGCCAGCCAGGAGCGCGCCTTCGACGAGGCGGCCGACGGCACCGCCCAGGGGTCTGGCGATCCCGATCCGACAACCTGA
GAGACCGGTCCGGGAAATGT

Downstream 100 bases:

>100_bases
GGAGAGAGATCGCGCATGTCCCGAGAAATCATCCACACCGACGCCGCCCCGGCCGCCATTGGTCCCTACAGCCAGGCGGT
GCGCGCCGGCGACTTCGTCT

Product: (p)ppGpp synthetase I SpoT/RelA

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 729; Mature: 728

Protein sequence:

>729_residues
MARAASVINAGESASASDPERRVQEVCALLERYLEPHQIEQVYDAYRFSAAAHEGQRRRSGEPYITHPIAVARILAEMRL
DAEAVVAALLHDVIEDTPTAKERIAERFGEQVAELVDGVSKLGAIDFQSKEEAQAESFRKMILAMAKDIRVILIKLADRL
HNMRTIWIMRPEKRRRIARETLDIYAPIAQRLGINSLRTELEDLGFAAHYPLRYRALKEKLARQRGRRGVILDTVCDQVR
TRLEQAGIQAEVVGREKHLWSIYCKMAQKQLNFRDVFDVYGFRVVVDTVDECYRVLGILHGLYKPRPGRIKDYIAIPKAN
GYQSLHTTLVGPHRIRLEAQIRTRDMHAVAESGIAAHWFYKDAGESGNTAQVRARQWVKDLLEMQRSVGTSEEFIESVKI
DLVPDEIYVFTPKGDILELPSNATPVDLAYAIHSDVGNSCIAAKVDHRLTPLRTPLQSGQMVEIITAPVGRPNPVWLDFV
VTAKARTAIRHALKRLRDDEAIELGRRMVERALSALSVDLDALDPARVDEALAGTGLASLDDLFRDVGLGNRVPWLVAQQ
LSGLEQGEAELEERPEEPEPHGTLTIRGNEGTVVSFARCCRPIPGDPIVGFVSTGRGVVIHHRDCKNVASQRKRSESWVD
VDWEADVDAEFPTLIGIDVVNERGALAQLAHGISAQGASIENVTIEERDGMIATVRFLILVRDRRHLAAVVRHLRAMPPV
QRIVRKKDG

Sequences:

>Translated_729_residues
MARAASVINAGESASASDPERRVQEVCALLERYLEPHQIEQVYDAYRFSAAAHEGQRRRSGEPYITHPIAVARILAEMRL
DAEAVVAALLHDVIEDTPTAKERIAERFGEQVAELVDGVSKLGAIDFQSKEEAQAESFRKMILAMAKDIRVILIKLADRL
HNMRTIWIMRPEKRRRIARETLDIYAPIAQRLGINSLRTELEDLGFAAHYPLRYRALKEKLARQRGRRGVILDTVCDQVR
TRLEQAGIQAEVVGREKHLWSIYCKMAQKQLNFRDVFDVYGFRVVVDTVDECYRVLGILHGLYKPRPGRIKDYIAIPKAN
GYQSLHTTLVGPHRIRLEAQIRTRDMHAVAESGIAAHWFYKDAGESGNTAQVRARQWVKDLLEMQRSVGTSEEFIESVKI
DLVPDEIYVFTPKGDILELPSNATPVDLAYAIHSDVGNSCIAAKVDHRLTPLRTPLQSGQMVEIITAPVGRPNPVWLDFV
VTAKARTAIRHALKRLRDDEAIELGRRMVERALSALSVDLDALDPARVDEALAGTGLASLDDLFRDVGLGNRVPWLVAQQ
LSGLEQGEAELEERPEEPEPHGTLTIRGNEGTVVSFARCCRPIPGDPIVGFVSTGRGVVIHHRDCKNVASQRKRSESWVD
VDWEADVDAEFPTLIGIDVVNERGALAQLAHGISAQGASIENVTIEERDGMIATVRFLILVRDRRHLAAVVRHLRAMPPV
QRIVRKKDG
>Mature_728_residues
ARAASVINAGESASASDPERRVQEVCALLERYLEPHQIEQVYDAYRFSAAAHEGQRRRSGEPYITHPIAVARILAEMRLD
AEAVVAALLHDVIEDTPTAKERIAERFGEQVAELVDGVSKLGAIDFQSKEEAQAESFRKMILAMAKDIRVILIKLADRLH
NMRTIWIMRPEKRRRIARETLDIYAPIAQRLGINSLRTELEDLGFAAHYPLRYRALKEKLARQRGRRGVILDTVCDQVRT
RLEQAGIQAEVVGREKHLWSIYCKMAQKQLNFRDVFDVYGFRVVVDTVDECYRVLGILHGLYKPRPGRIKDYIAIPKANG
YQSLHTTLVGPHRIRLEAQIRTRDMHAVAESGIAAHWFYKDAGESGNTAQVRARQWVKDLLEMQRSVGTSEEFIESVKID
LVPDEIYVFTPKGDILELPSNATPVDLAYAIHSDVGNSCIAAKVDHRLTPLRTPLQSGQMVEIITAPVGRPNPVWLDFVV
TAKARTAIRHALKRLRDDEAIELGRRMVERALSALSVDLDALDPARVDEALAGTGLASLDDLFRDVGLGNRVPWLVAQQL
SGLEQGEAELEERPEEPEPHGTLTIRGNEGTVVSFARCCRPIPGDPIVGFVSTGRGVVIHHRDCKNVASQRKRSESWVDV
DWEADVDAEFPTLIGIDVVNERGALAQLAHGISAQGASIENVTIEERDGMIATVRFLILVRDRRHLAAVVRHLRAMPPVQ
RIVRKKDG

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=136, Percent_Identity=38.2352941176471, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1790082, Length=700, Percent_Identity=53.2857142857143, Blast_Score=789, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=686, Percent_Identity=32.798833819242, Blast_Score=373, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25143118, Length=128, Percent_Identity=34.375, Blast_Score=66, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24650996, Length=190, Percent_Identity=31.5789473684211, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 81627; Mature: 81495

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAASVINAGESASASDPERRVQEVCALLERYLEPHQIEQVYDAYRFSAAAHEGQRRRS
CCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
GEPYITHPIAVARILAEMRLDAEAVVAALLHDVIEDTPTAKERIAERFGEQVAELVDGVS
CCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
KLGAIDFQSKEEAQAESFRKMILAMAKDIRVILIKLADRLHNMRTIWIMRPEKRRRIARE
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHH
TLDIYAPIAQRLGINSLRTELEDLGFAAHYPLRYRALKEKLARQRGRRGVILDTVCDQVR
HHHHHHHHHHHCCHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
TRLEQAGIQAEVVGREKHLWSIYCKMAQKQLNFRDVFDVYGFRVVVDTVDECYRVLGILH
HHHHHCCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLYKPRPGRIKDYIAIPKANGYQSLHTTLVGPHRIRLEAQIRTRDMHAVAESGIAAHWFY
HHCCCCCCCCCCEEECCCCCCCHHHHHHHCCCEEEEEEEEHHHHHHHHHHHCCCEEEEEE
KDAGESGNTAQVRARQWVKDLLEMQRSVGTSEEFIESVKIDLVPDEIYVFTPKGDILELP
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCCEEEECCCCCEEECC
SNATPVDLAYAIHSDVGNSCIAAKVDHRLTPLRTPLQSGQMVEIITAPVGRPNPVWLDFV
CCCCCHHHHHHHHHCCCCCEEEHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEE
VTAKARTAIRHALKRLRDDEAIELGRRMVERALSALSVDLDALDPARVDEALAGTGLASL
EEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHH
DDLFRDVGLGNRVPWLVAQQLSGLEQGEAELEERPEEPEPHGTLTIRGNEGTVVSFARCC
HHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHC
RPIPGDPIVGFVSTGRGVVIHHRDCKNVASQRKRSESWVDVDWEADVDAEFPTLIGIDVV
CCCCCCCEEEEEECCCEEEEECCHHHHHHHHHHCCCCCEECCCCCCCCCCCCCEEEEEEE
NERGALAQLAHGISAQGASIENVTIEERDGMIATVRFLILVRDRRHLAAVVRHLRAMPPV
CCCCHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCHH
QRIVRKKDG
HHHHHCCCC
>Mature Secondary Structure 
ARAASVINAGESASASDPERRVQEVCALLERYLEPHQIEQVYDAYRFSAAAHEGQRRRS
CCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
GEPYITHPIAVARILAEMRLDAEAVVAALLHDVIEDTPTAKERIAERFGEQVAELVDGVS
CCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
KLGAIDFQSKEEAQAESFRKMILAMAKDIRVILIKLADRLHNMRTIWIMRPEKRRRIARE
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHH
TLDIYAPIAQRLGINSLRTELEDLGFAAHYPLRYRALKEKLARQRGRRGVILDTVCDQVR
HHHHHHHHHHHCCHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
TRLEQAGIQAEVVGREKHLWSIYCKMAQKQLNFRDVFDVYGFRVVVDTVDECYRVLGILH
HHHHHCCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLYKPRPGRIKDYIAIPKANGYQSLHTTLVGPHRIRLEAQIRTRDMHAVAESGIAAHWFY
HHCCCCCCCCCCEEECCCCCCCHHHHHHHCCCEEEEEEEEHHHHHHHHHHHCCCEEEEEE
KDAGESGNTAQVRARQWVKDLLEMQRSVGTSEEFIESVKIDLVPDEIYVFTPKGDILELP
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCCEEEECCCCCEEECC
SNATPVDLAYAIHSDVGNSCIAAKVDHRLTPLRTPLQSGQMVEIITAPVGRPNPVWLDFV
CCCCCHHHHHHHHHCCCCCEEEHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEE
VTAKARTAIRHALKRLRDDEAIELGRRMVERALSALSVDLDALDPARVDEALAGTGLASL
EEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHH
DDLFRDVGLGNRVPWLVAQQLSGLEQGEAELEERPEEPEPHGTLTIRGNEGTVVSFARCC
HHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHC
RPIPGDPIVGFVSTGRGVVIHHRDCKNVASQRKRSESWVDVDWEADVDAEFPTLIGIDVV
CCCCCCCEEEEEECCCEEEEECCHHHHHHHHHHCCCCCEECCCCCCCCCCCCCEEEEEEE
NERGALAQLAHGISAQGASIENVTIEERDGMIATVRFLILVRDRRHLAAVVRHLRAMPPV
CCCCHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCHH
QRIVRKKDG
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]