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The map label for this gene is bioF

Identifier: 121997753

GI number: 121997753

Start: 1033711

End: 1034919

Strand: Reverse

Name: bioF

Synonym: Hhal_0962

Alternate gene names: 121997753

Gene position: 1034919-1033711 (Counterclockwise)

Preceding gene: 121997754

Following gene: 121997752

Centisome position: 38.64

GC content: 75.52

Gene sequence:

>1209_bases
GTGAACCACCCGGCGGCCCGACTCGATGCGCGGTTGGCCCCGCGTCTTGCCGAGCGCCGCCAGGCCGGGCTCTATCGCTG
CGCCGAAGCCCTAGAGGACTACGATGGCGCCACCGCCGTGCGGTCCGATGGCCAGCGCTGCACGGTCTTCTGCGGTAACG
ACTACCTGGGCCTGGCCGCCGATCCGCGCCCGGCTCGCGCCCTGGCCGAGCACGCCCGGCGCCAGGGCAGTGGTGCCGGC
GCTGCGCACCTGGTCACCGGCCACCGCCCCGAGCACGAGGCGCTAGAGGCGGAGCTGGCGGCGTTCACCGGCCGGGAGGC
GGCGCTGCTCTTCTCCACGGGCTACATGGCCAACCTCGGTGTCGTGACCACCCTGGTCCGGCGGGGCCAGGCGGTGGCCG
AGGACCGGCTTAACCACGCCTCGCTGATCGACGCCGTGCGTCTGGCCGGCGGACGGCCACGCCGCTACCGCCACGCCGAT
CCCACTCACCTGGCCCAGCGCCTCGACGATCGCGCCCAGCTCGTGGTCACCGACGGGGTATTCAGCATGGACGGCGATGT
GGCCCCCTTGCCCGAACTGGCCGCGGCGGCCGAGGCGACCGGCGCGGCGCTGGTGGTCGACGATGCCCACGGCCTGGGGG
TGATCGGCCCGGAGGGCGGCGGCAGCGTCCCCGCCACCGGCTGTGCCCCGGAACGGGTGCCGGTGCTGGTGGGGACGCTG
GGCAAGGCCTTCGGTACCTTCGGCGCCTTCGTCGCCGGCAGCCGGACCCTCATCGATGCCCTGCGCCAGTGGGCGCGCCC
GTATATCTACACCACCGCGCCCCCGCCGGCGCAGGCGGCGGCGGCCCTGGAGGCGGTGCGCATCGCCCGGGCCGAGGGGT
GGCGGCGCGAGCATCTGCAGGCGCTGATCGCCCGCTTCCGCAGCGGCGCCCGGCAGCTCGGTCTCCCGGTGCCACAGCCC
GCGGGGCCAAGGACGCCGATCCAGCCGGTGCTGGTCGGCCCTAGTGCTACGGCCACCGCCTGGAGTGCCGAACTCGACCG
CCGCGGGCTGCGCGTGGCGGCGATCCGTCCGCCGACGGTGCCGGCGGGGACAGCCCGGCTGCGCGTCAGCCTGACCGCCT
GTCACAGTGAGGAGGATGTCGATCGGCTGCTGGAGGCCTTGGCCGCGGCGGCGCGCCAGGCCCCGCCCACCCTGGAGGCG
CCGTCGTGA

Upstream 100 bases:

>100_bases
TGACCACCGACAACCCGGACGCCGACGCCGACCATCGGCTGCTCCACCGCCTGGGCATGACCCTGGAGCCGCGCACCCAC
AGCTGCGCCGAGCTGGAGCC

Downstream 100 bases:

>100_bases
CGCCGCTGCTGCTGGTGCCGGGGTGGTCGTTCGATGGTCGGGTCTTTGATCGCCTCCGGTCGGTGCTGGATACTCCGGCG
CAGGTGGTCGAGCTGCCCGG

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MNHPAARLDARLAPRLAERRQAGLYRCAEALEDYDGATAVRSDGQRCTVFCGNDYLGLAADPRPARALAEHARRQGSGAG
AAHLVTGHRPEHEALEAELAAFTGREAALLFSTGYMANLGVVTTLVRRGQAVAEDRLNHASLIDAVRLAGGRPRRYRHAD
PTHLAQRLDDRAQLVVTDGVFSMDGDVAPLPELAAAAEATGAALVVDDAHGLGVIGPEGGGSVPATGCAPERVPVLVGTL
GKAFGTFGAFVAGSRTLIDALRQWARPYIYTTAPPPAQAAAALEAVRIARAEGWRREHLQALIARFRSGARQLGLPVPQP
AGPRTPIQPVLVGPSATATAWSAELDRRGLRVAAIRPPTVPAGTARLRVSLTACHSEEDVDRLLEALAAAARQAPPTLEA
PS

Sequences:

>Translated_402_residues
MNHPAARLDARLAPRLAERRQAGLYRCAEALEDYDGATAVRSDGQRCTVFCGNDYLGLAADPRPARALAEHARRQGSGAG
AAHLVTGHRPEHEALEAELAAFTGREAALLFSTGYMANLGVVTTLVRRGQAVAEDRLNHASLIDAVRLAGGRPRRYRHAD
PTHLAQRLDDRAQLVVTDGVFSMDGDVAPLPELAAAAEATGAALVVDDAHGLGVIGPEGGGSVPATGCAPERVPVLVGTL
GKAFGTFGAFVAGSRTLIDALRQWARPYIYTTAPPPAQAAAALEAVRIARAEGWRREHLQALIARFRSGARQLGLPVPQP
AGPRTPIQPVLVGPSATATAWSAELDRRGLRVAAIRPPTVPAGTARLRVSLTACHSEEDVDRLLEALAAAARQAPPTLEA
PS
>Mature_402_residues
MNHPAARLDARLAPRLAERRQAGLYRCAEALEDYDGATAVRSDGQRCTVFCGNDYLGLAADPRPARALAEHARRQGSGAG
AAHLVTGHRPEHEALEAELAAFTGREAALLFSTGYMANLGVVTTLVRRGQAVAEDRLNHASLIDAVRLAGGRPRRYRHAD
PTHLAQRLDDRAQLVVTDGVFSMDGDVAPLPELAAAAEATGAALVVDDAHGLGVIGPEGGGSVPATGCAPERVPVLVGTL
GKAFGTFGAFVAGSRTLIDALRQWARPYIYTTAPPPAQAAAALEAVRIARAEGWRREHLQALIARFRSGARQLGLPVPQP
AGPRTPIQPVLVGPSATATAWSAELDRRGLRVAAIRPPTVPAGTARLRVSLTACHSEEDVDRLLEALAAAARQAPPTLEA
PS

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=394, Percent_Identity=35.7868020304569, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI284448556, Length=348, Percent_Identity=37.3563218390805, Blast_Score=229, Evalue=5e-60,
Organism=Homo sapiens, GI83977444, Length=403, Percent_Identity=33.0024813895782, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI83977442, Length=403, Percent_Identity=33.0024813895782, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI83977440, Length=403, Percent_Identity=33.0024813895782, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI4502025, Length=408, Percent_Identity=34.8039215686275, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI40316939, Length=408, Percent_Identity=34.8039215686275, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI4758668, Length=376, Percent_Identity=28.1914893617021, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI119220554, Length=354, Percent_Identity=27.9661016949153, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI5454084, Length=252, Percent_Identity=23.4126984126984, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI1786993, Length=351, Percent_Identity=45.2991452991453, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1790046, Length=390, Percent_Identity=34.1025641025641, Blast_Score=217, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI71994529, Length=392, Percent_Identity=32.9081632653061, Blast_Score=234, Evalue=7e-62,
Organism=Caenorhabditis elegans, GI32566772, Length=354, Percent_Identity=27.9661016949153, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17560914, Length=376, Percent_Identity=28.1914893617021, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17560912, Length=376, Percent_Identity=28.1914893617021, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71982617, Length=323, Percent_Identity=22.2910216718266, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71982625, Length=323, Percent_Identity=22.2910216718266, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320438, Length=374, Percent_Identity=33.9572192513369, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6320267, Length=358, Percent_Identity=28.4916201117318, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6323954, Length=265, Percent_Identity=23.3962264150943, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24662918, Length=397, Percent_Identity=34.0050377833753, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI17137420, Length=365, Percent_Identity=34.7945205479452, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI17136286, Length=382, Percent_Identity=29.0575916230367, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24653280, Length=276, Percent_Identity=22.1014492753623, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24653276, Length=276, Percent_Identity=22.1014492753623, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24653278, Length=276, Percent_Identity=22.1014492753623, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_HALHL (A1WVM6)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002540.1
- ProteinModelPortal:   A1WVM6
- STRING:   A1WVM6
- GeneID:   4709463
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_0962
- eggNOG:   COG0156
- HOGENOM:   HBG649839
- OMA:   ANAQFAN
- PhylomeDB:   A1WVM6
- ProtClustDB:   CLSK727636
- BioCyc:   HHAL349124:HHAL_0962-MONOMER
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 42223; Mature: 42223

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 26-26 BINDING 139-139 BINDING 182-182 BINDING 359-359

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHPAARLDARLAPRLAERRQAGLYRCAEALEDYDGATAVRSDGQRCTVFCGNDYLGLAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCEEECCC
DPRPARALAEHARRQGSGAGAAHLVTGHRPEHEALEAELAAFTGREAALLFSTGYMANLG
CCCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHH
VVTTLVRRGQAVAEDRLNHASLIDAVRLAGGRPRRYRHADPTHLAQRLDDRAQLVVTDGV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCE
FSMDGDVAPLPELAAAAEATGAALVVDDAHGLGVIGPEGGGSVPATGCAPERVPVLVGTL
EECCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHH
GKAFGTFGAFVAGSRTLIDALRQWARPYIYTTAPPPAQAAAALEAVRIARAEGWRREHLQ
HHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHH
ALIARFRSGARQLGLPVPQPAGPRTPIQPVLVGPSATATAWSAELDRRGLRVAAIRPPTV
HHHHHHHHCHHHCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEECCCCC
PAGTARLRVSLTACHSEEDVDRLLEALAAAARQAPPTLEAPS
CCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNHPAARLDARLAPRLAERRQAGLYRCAEALEDYDGATAVRSDGQRCTVFCGNDYLGLAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCEEECCC
DPRPARALAEHARRQGSGAGAAHLVTGHRPEHEALEAELAAFTGREAALLFSTGYMANLG
CCCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHH
VVTTLVRRGQAVAEDRLNHASLIDAVRLAGGRPRRYRHADPTHLAQRLDDRAQLVVTDGV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCE
FSMDGDVAPLPELAAAAEATGAALVVDDAHGLGVIGPEGGGSVPATGCAPERVPVLVGTL
EECCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHH
GKAFGTFGAFVAGSRTLIDALRQWARPYIYTTAPPPAQAAAALEAVRIARAEGWRREHLQ
HHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHH
ALIARFRSGARQLGLPVPQPAGPRTPIQPVLVGPSATATAWSAELDRRGLRVAAIRPPTV
HHHHHHHHCHHHCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEECCCCC
PAGTARLRVSLTACHSEEDVDRLLEALAAAARQAPPTLEAPS
CCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA