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The map label for this gene is bioH [H]

Identifier: 121997752

GI number: 121997752

Start: 1033013

End: 1033714

Strand: Reverse

Name: bioH [H]

Synonym: Hhal_0961

Alternate gene names: 121997752

Gene position: 1033714-1033013 (Counterclockwise)

Preceding gene: 121997753

Following gene: 121997751

Centisome position: 38.59

GC content: 74.5

Gene sequence:

>702_bases
GTGACGCCGCTGCTGCTGGTGCCGGGGTGGTCGTTCGATGGTCGGGTCTTTGATCGCCTCCGGTCGGTGCTGGATACTCC
GGCGCAGGTGGTCGAGCTGCCCGGCCACGGCGGGGCCGCCGATCCCCGCTACGAAGACCTCGCTGGGGTCGCCACGCCGG
TGATCGATGCGGCCCCGGACGACGCCGTATGGGTGGGCTGGTCGCTGGGTGGCACCGTCGCCCTCGAGGCACTGCGCCGC
GGCAAGCCCCTGCGCGGTGTGGCCCTGATCGGCGCGACGCCACGGTTTACCATGGATATCGACTGGCCCTGCGCCGTCCC
CGCCGCGGAGCTGACGGCCATGCGAGCCGGTCTGGCGCGGAGTCCTGCCGCCACGGTGCGGCGCTTCCGTCGTCGGCTGG
GGCAGGTCTCCGCCGCCGATGCAGCCGCCGTTACCGACCCCTGCACGGCGTCGCCGGTCGGGCTGGCGGCGGGGCTCGCC
GCGTTGGCCGAGGCCGACCTGCGCCAGGCGCCGGCCGGAGCTGTGCCGTCGCTGTGGCTCGGCGGTGGCCAGGACCCGCT
GGTGCCTCCGGCGGCGCTGCAGCGCGCGGCGGAGGGCTGCGACGGCCAGCTGCGGATCCTGGAGGGGGCCGGGCATCTGC
CCCACTGCACCCACGCCACCGCAGTGGCGCAGACGATCCACGCTTTCCTTGAGGAGATCTGA

Upstream 100 bases:

>100_bases
TCAGCCTGACCGCCTGTCACAGTGAGGAGGATGTCGATCGGCTGCTGGAGGCCTTGGCCGCGGCGGCGCGCCAGGCCCCG
CCCACCCTGGAGGCGCCGTC

Downstream 100 bases:

>100_bases
CCGTGAACCCAGGCGACGCCCAGTCGATTGATCGCCGCTGCGTGCGACGCAGCTTCGACGCCTCGGCGGAGCGCTACGAC
GAGGTCGCCGTGCTCCAGCG

Product: alpha/beta hydrolase fold

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MTPLLLVPGWSFDGRVFDRLRSVLDTPAQVVELPGHGGAADPRYEDLAGVATPVIDAAPDDAVWVGWSLGGTVALEALRR
GKPLRGVALIGATPRFTMDIDWPCAVPAAELTAMRAGLARSPAATVRRFRRRLGQVSAADAAAVTDPCTASPVGLAAGLA
ALAEADLRQAPAGAVPSLWLGGGQDPLVPPAALQRAAEGCDGQLRILEGAGHLPHCTHATAVAQTIHAFLEEI

Sequences:

>Translated_233_residues
MTPLLLVPGWSFDGRVFDRLRSVLDTPAQVVELPGHGGAADPRYEDLAGVATPVIDAAPDDAVWVGWSLGGTVALEALRR
GKPLRGVALIGATPRFTMDIDWPCAVPAAELTAMRAGLARSPAATVRRFRRRLGQVSAADAAAVTDPCTASPVGLAAGLA
ALAEADLRQAPAGAVPSLWLGGGQDPLVPPAALQRAAEGCDGQLRILEGAGHLPHCTHATAVAQTIHAFLEEI
>Mature_232_residues
TPLLLVPGWSFDGRVFDRLRSVLDTPAQVVELPGHGGAADPRYEDLAGVATPVIDAAPDDAVWVGWSLGGTVALEALRRG
KPLRGVALIGATPRFTMDIDWPCAVPAAELTAMRAGLARSPAATVRRFRRRLGQVSAADAAAVTDPCTASPVGLAAGLAA
LAEADLRQAPAGAVPSLWLGGGQDPLVPPAALQRAAEGCDGQLRILEGAGHLPHCTHATAVAQTIHAFLEEI

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=242, Percent_Identity=28.9256198347107, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24046; Mature: 23915

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLLLVPGWSFDGRVFDRLRSVLDTPAQVVELPGHGGAADPRYEDLAGVATPVIDAAPD
CCCEEEECCCCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCC
DAVWVGWSLGGTVALEALRRGKPLRGVALIGATPRFTMDIDWPCAVPAAELTAMRAGLAR
CCEEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCEEEECCCCCCCCHHHHHHHHHCCCC
SPAATVRRFRRRLGQVSAADAAAVTDPCTASPVGLAAGLAALAEADLRQAPAGAVPSLWL
CHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEE
GGGQDPLVPPAALQRAAEGCDGQLRILEGAGHLPHCTHATAVAQTIHAFLEEI
CCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TPLLLVPGWSFDGRVFDRLRSVLDTPAQVVELPGHGGAADPRYEDLAGVATPVIDAAPD
CCEEEECCCCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCC
DAVWVGWSLGGTVALEALRRGKPLRGVALIGATPRFTMDIDWPCAVPAAELTAMRAGLAR
CCEEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCEEEECCCCCCCCHHHHHHHHHCCCC
SPAATVRRFRRRLGQVSAADAAAVTDPCTASPVGLAAGLAALAEADLRQAPAGAVPSLWL
CHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEE
GGGQDPLVPPAALQRAAEGCDGQLRILEGAGHLPHCTHATAVAQTIHAFLEEI
CCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]