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The map label for this gene is bioB

Identifier: 121997754

GI number: 121997754

Start: 1034916

End: 1035896

Strand: Reverse

Name: bioB

Synonym: Hhal_0963

Alternate gene names: 121997754

Gene position: 1035896-1034916 (Counterclockwise)

Preceding gene: 121997756

Following gene: 121997753

Centisome position: 38.68

GC content: 71.36

Gene sequence:

>981_bases
ATGACTGAGACCGACACCTGGCACTGGGATCAGCCGCAGATCCACGCCTTGTTCGAGCTGCCCCTGCCGGAGCTGCTGTT
CCGCGCCCAGGAAGTGCATCGCCGTCACTTTAACCCGGGGCAGGTCCAGGCCTGCACCCTGGTCTCGATCAAGACCGGCG
CCTGCGCCGAGGACTGCACCTACTGCTCCCAGAGCGCGCGGTACGACACCGGCCTGGAGCGCGAGGCGCTGATCGATGTC
GAGACCGTCCGCGAGGCGGCCCAGCGGGCTCGGGCCAGCGGGGCCACCCGGCTGTGCATGGGTGCGGCCTGGCGCGGCCC
CAAAGACCGCGACCTGGAGACGCTGGTAGCCATGGTCCGGGCGGTTAAGGCCGAAGGCCTGGAGGCCTGCCTCTCTGCCG
GCCTGCTCGCCGAGGGGCAGGCCGAGCGGCTGGCCGAGGCCGGGCTCGACTACTTCAACCACAACCTCGACACCTCGCCG
TCGTACTACGACCAGGTGGTGACCACCCGCAGCTACGAGCAGCGGCTGCAGACGCTTGAGCGCATCCGCGACGCCGGGAT
GCGGGTCTGCTGCGGTGGCATCGTCGGCCTGGGGGAGGCGCGGGCGGACCGCGTCGAGATGCTCGCCACCCTGGCCAACC
TGCCGGTGCCGCCGCAGAGCGTGCCGATCAACCGGCTGATCCCCATCCCCGGGACCCCGCTGGAGGCGGCCGAGCCGGTG
GATCCGTTCGAGATCGTGCGCACCATCGCCGCCACGCGCCTGGTCCTGCCGCGCTCCTACGTCCGCCTGGCGGCCGGTCG
CGAGCAGATGAGCGACGAGCTCCAGGCGCTGTGCCTGAGCGCCGGTGCCAACAGTCTGTTCCTCGGCGAGCGGCTGCTGA
CCACCGACAACCCGGACGCCGACGCCGACCATCGGCTGCTCCACCGCCTGGGCATGACCCTGGAGCCGCGCACCCACAGC
TGCGCCGAGCTGGAGCCGTGA

Upstream 100 bases:

>100_bases
GACTGAGTGCATAGCGTCATAGTTGACCATGGTCGCCTCCTTGCTACCATAAGCGCCTCCTGCGCGGCACGCCCCGCGGT
CAACCTGCAGTGAGCCGATC

Downstream 100 bases:

>100_bases
ACCACCCGGCGGCCCGACTCGATGCGCGGTTGGCCCCGCGTCTTGCCGAGCGCCGCCAGGCCGGGCTCTATCGCTGCGCC
GAAGCCCTAGAGGACTACGA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTETDTWHWDQPQIHALFELPLPELLFRAQEVHRRHFNPGQVQACTLVSIKTGACAEDCTYCSQSARYDTGLEREALIDV
ETVREAAQRARASGATRLCMGAAWRGPKDRDLETLVAMVRAVKAEGLEACLSAGLLAEGQAERLAEAGLDYFNHNLDTSP
SYYDQVVTTRSYEQRLQTLERIRDAGMRVCCGGIVGLGEARADRVEMLATLANLPVPPQSVPINRLIPIPGTPLEAAEPV
DPFEIVRTIAATRLVLPRSYVRLAAGREQMSDELQALCLSAGANSLFLGERLLTTDNPDADADHRLLHRLGMTLEPRTHS
CAELEP

Sequences:

>Translated_326_residues
MTETDTWHWDQPQIHALFELPLPELLFRAQEVHRRHFNPGQVQACTLVSIKTGACAEDCTYCSQSARYDTGLEREALIDV
ETVREAAQRARASGATRLCMGAAWRGPKDRDLETLVAMVRAVKAEGLEACLSAGLLAEGQAERLAEAGLDYFNHNLDTSP
SYYDQVVTTRSYEQRLQTLERIRDAGMRVCCGGIVGLGEARADRVEMLATLANLPVPPQSVPINRLIPIPGTPLEAAEPV
DPFEIVRTIAATRLVLPRSYVRLAAGREQMSDELQALCLSAGANSLFLGERLLTTDNPDADADHRLLHRLGMTLEPRTHS
CAELEP
>Mature_325_residues
TETDTWHWDQPQIHALFELPLPELLFRAQEVHRRHFNPGQVQACTLVSIKTGACAEDCTYCSQSARYDTGLEREALIDVE
TVREAAQRARASGATRLCMGAAWRGPKDRDLETLVAMVRAVKAEGLEACLSAGLLAEGQAERLAEAGLDYFNHNLDTSPS
YYDQVVTTRSYEQRLQTLERIRDAGMRVCCGGIVGLGEARADRVEMLATLANLPVPPQSVPINRLIPIPGTPLEAAEPVD
PFEIVRTIAATRLVLPRSYVRLAAGREQMSDELQALCLSAGANSLFLGERLLTTDNPDADADHRLLHRLGMTLEPRTHSC
AELEP

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=305, Percent_Identity=59.344262295082, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321725, Length=313, Percent_Identity=43.7699680511182, Blast_Score=292, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_HALHL (A1WVM7)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002541.1
- ProteinModelPortal:   A1WVM7
- SMR:   A1WVM7
- STRING:   A1WVM7
- GeneID:   4709464
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_0963
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   A1WVM7
- BioCyc:   HHAL349124:HHAL_0963-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35926; Mature: 35795

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETDTWHWDQPQIHALFELPLPELLFRAQEVHRRHFNPGQVQACTLVSIKTGACAEDCT
CCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHH
YCSQSARYDTGLEREALIDVETVREAAQRARASGATRLCMGAAWRGPKDRDLETLVAMVR
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHH
AVKAEGLEACLSAGLLAEGQAERLAEAGLDYFNHNLDTSPSYYDQVVTTRSYEQRLQTLE
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RIRDAGMRVCCGGIVGLGEARADRVEMLATLANLPVPPQSVPINRLIPIPGTPLEAAEPV
HHHHCCCEEHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCC
DPFEIVRTIAATRLVLPRSYVRLAAGREQMSDELQALCLSAGANSLFLGERLLTTDNPDA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCC
DADHRLLHRLGMTLEPRTHSCAELEP
CHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TETDTWHWDQPQIHALFELPLPELLFRAQEVHRRHFNPGQVQACTLVSIKTGACAEDCT
CCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHH
YCSQSARYDTGLEREALIDVETVREAAQRARASGATRLCMGAAWRGPKDRDLETLVAMVR
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHH
AVKAEGLEACLSAGLLAEGQAERLAEAGLDYFNHNLDTSPSYYDQVVTTRSYEQRLQTLE
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RIRDAGMRVCCGGIVGLGEARADRVEMLATLANLPVPPQSVPINRLIPIPGTPLEAAEPV
HHHHCCCEEHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCC
DPFEIVRTIAATRLVLPRSYVRLAAGREQMSDELQALCLSAGANSLFLGERLLTTDNPDA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCC
DADHRLLHRLGMTLEPRTHSCAELEP
CHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA