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The map label for this gene is argG

Identifier: 121997522

GI number: 121997522

Start: 810528

End: 811739

Strand: Reverse

Name: argG

Synonym: Hhal_0725

Alternate gene names: 121997522

Gene position: 811739-810528 (Counterclockwise)

Preceding gene: 121997523

Following gene: 121997515

Centisome position: 30.31

GC content: 69.47

Gene sequence:

>1212_bases
ATGTCGGACGTCAAGAAGGTCGTACTCGCCTACTCCGGCGGTCTCGACACCTCCGTGATCCTCAAGTGGCTCGAGGAGAC
CTACGGCTGCGAGGTGGTGACATTCACCGCCGATCTGGGCCAGGGCGAGGAGCTGGAGCCCGCCCGGCGCAAGGCGGAGG
CGTTCGGGATCCGCGAGATCTACGTCGATGATCTGCGCGAGGAGTTCGCCCGCGACTTCGTCTTCCCGATGTTCCGCGCC
AACGCCGTCTACGAGGGCGAGTACCTGCTCGGCACCTCCATCGCCCGGCCGCTGATCGCCAAGCGGCAGGTGGAGATCGC
CCGCGAGACCGGCGCCGACGCCGTCGCCCACGGCGCCACCGGCAAGGGCAATGATCAGGTCCGCTTCGAGCTCGGCTACT
ACGGCCTCGAGCCGGGGATCAAGGTCATCGCCCCGTGGCGGGAGTGGGACCTCAACTCCCGCGAGCGGCTCCTGCAGTAC
GCCGAGCGCCACGGCATCGACATCGAGGGCAAGCGCGAGGAGGGCGGCGCGCCGTACTCCATGGACGCCAACCTGCTGCA
CATCTCCTACGAGGGCGGGGTCCTGGAGGACACCTGGACCGAGGCCGAGGAGGCGATGTGGCTGTGGACCAACGCGCCGG
AGGCGGCGCCGGACGAGCCGCAGTACATCGACATCGCCTTCGCCGGTGGCGATCCGGTGGCCATCGACGGCGAGCGGCTC
TCGCCGGCGGCGCTGCTCGGCCGGCTCAACGACCTGGGGGCGCGCCACGGCATCGGCCGGATCGACATCGTCGAGAACCG
CTACGTGGGCATGAAGTCCCGCGGCTGCTACGAGACCCCCGGCGGCACCATCCTGCTGCGCGCCCACCGTGCCATGGAGT
CGATCACCCTGGATCGCGATGCGGCGCACCTGAAGGACGAGCTGATGCCGCGCTACGCCGAGCTGGTCTACAACGGCTAC
TGGTTCGCCCCGGAGCGCGAGGCGCTGCAGGCGATGATCGACCAGACCCAGCAGGACGTCGAGGGCACCGTGCGCCTGAA
GCTCTACAAGGGCAACGTCATCGTCGTCGGCCGCCAGTCGCCGAAGAGCCTCTTCGACGCCTCGGTGGCCACCTTCGAGG
ACGACGCCGGCGCCTACGACCAGAAGGACGCCGAGGGCTTCATCCGCCTCAACGCCCTGCGGCTGCGCCTCGGCGCCAAG
CGCCGCGGCTGA

Upstream 100 bases:

>100_bases
CTGGGACGAGGCGGAGAATCGCCTGCACGCCCAGAAGGCGCTGCTTGAATTCCTGATGCTGGGATACTGACCCCTGAACC
TGAATACCGAGGAGCTCCCC

Downstream 100 bases:

>100_bases
CCCCGGCGCGGAGGCACCGCTGCCGGCGCCCCGTCGGTAGCGGTGCCGGTGGGCTGCGCCCCCTCATGGCGCCGTGGCCG
GTGCCCCCACCTCTTAGGTC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSDVKKVVLAYSGGLDTSVILKWLEETYGCEVVTFTADLGQGEELEPARRKAEAFGIREIYVDDLREEFARDFVFPMFRA
NAVYEGEYLLGTSIARPLIAKRQVEIARETGADAVAHGATGKGNDQVRFELGYYGLEPGIKVIAPWREWDLNSRERLLQY
AERHGIDIEGKREEGGAPYSMDANLLHISYEGGVLEDTWTEAEEAMWLWTNAPEAAPDEPQYIDIAFAGGDPVAIDGERL
SPAALLGRLNDLGARHGIGRIDIVENRYVGMKSRGCYETPGGTILLRAHRAMESITLDRDAAHLKDELMPRYAELVYNGY
WFAPEREALQAMIDQTQQDVEGTVRLKLYKGNVIVVGRQSPKSLFDASVATFEDDAGAYDQKDAEGFIRLNALRLRLGAK
RRG

Sequences:

>Translated_403_residues
MSDVKKVVLAYSGGLDTSVILKWLEETYGCEVVTFTADLGQGEELEPARRKAEAFGIREIYVDDLREEFARDFVFPMFRA
NAVYEGEYLLGTSIARPLIAKRQVEIARETGADAVAHGATGKGNDQVRFELGYYGLEPGIKVIAPWREWDLNSRERLLQY
AERHGIDIEGKREEGGAPYSMDANLLHISYEGGVLEDTWTEAEEAMWLWTNAPEAAPDEPQYIDIAFAGGDPVAIDGERL
SPAALLGRLNDLGARHGIGRIDIVENRYVGMKSRGCYETPGGTILLRAHRAMESITLDRDAAHLKDELMPRYAELVYNGY
WFAPEREALQAMIDQTQQDVEGTVRLKLYKGNVIVVGRQSPKSLFDASVATFEDDAGAYDQKDAEGFIRLNALRLRLGAK
RRG
>Mature_402_residues
SDVKKVVLAYSGGLDTSVILKWLEETYGCEVVTFTADLGQGEELEPARRKAEAFGIREIYVDDLREEFARDFVFPMFRAN
AVYEGEYLLGTSIARPLIAKRQVEIARETGADAVAHGATGKGNDQVRFELGYYGLEPGIKVIAPWREWDLNSRERLLQYA
ERHGIDIEGKREEGGAPYSMDANLLHISYEGGVLEDTWTEAEEAMWLWTNAPEAAPDEPQYIDIAFAGGDPVAIDGERLS
PAALLGRLNDLGARHGIGRIDIVENRYVGMKSRGCYETPGGTILLRAHRAMESITLDRDAAHLKDELMPRYAELVYNGYW
FAPEREALQAMIDQTQQDVEGTVRLKLYKGNVIVVGRQSPKSLFDASVATFEDDAGAYDQKDAEGFIRLNALRLRLGAKR
RG

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=405, Percent_Identity=47.1604938271605, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI16950633, Length=405, Percent_Identity=47.1604938271605, Blast_Score=367, Evalue=1e-102,
Organism=Escherichia coli, GI1789563, Length=370, Percent_Identity=27.027027027027, Blast_Score=112, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324514, Length=406, Percent_Identity=43.8423645320197, Blast_Score=340, Evalue=3e-94,
Organism=Drosophila melanogaster, GI21358151, Length=404, Percent_Identity=45.7920792079208, Blast_Score=335, Evalue=2e-92,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_HALHL (A1WUZ5)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002309.1
- ProteinModelPortal:   A1WUZ5
- SMR:   A1WUZ5
- STRING:   A1WUZ5
- GeneID:   4711348
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_0725
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- PhylomeDB:   A1WUZ5
- ProtClustDB:   PRK00509
- BioCyc:   HHAL349124:HHAL_0725-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44974; Mature: 44843

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 37-37 BINDING 88-88 BINDING 93-93 BINDING 118-118 BINDING 120-120 BINDING 124-124 BINDING 124-124 BINDING 125-125 BINDING 128-128 BINDING 180-180 BINDING 189-189 BINDING 265-265 BINDING 277-277

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVKKVVLAYSGGLDTSVILKWLEETYGCEVVTFTADLGQGEELEPARRKAEAFGIREI
CCCHHEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHCCHHHH
YVDDLREEFARDFVFPMFRANAVYEGEYLLGTSIARPLIAKRQVEIARETGADAVAHGAT
HHHHHHHHHHHHHHHHHHHCCEEECCCEEECHHHHHHHHHHHHHHHHHHCCCCHHCCCCC
GKGNDQVRFELGYYGLEPGIKVIAPWREWDLNSRERLLQYAERHGIDIEGKREEGGAPYS
CCCCCEEEEEEEEEECCCCCEEEECCHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEE
MDANLLHISYEGGVLEDTWTEAEEAMWLWTNAPEAAPDEPQYIDIAFAGGDPVAIDGERL
ECCCEEEEEECCCCCCCCCCCHHCEEEEEECCCCCCCCCCCEEEEEEECCCEEEECCCCC
SPAALLGRLNDLGARHGIGRIDIVENRYVGMKSRGCYETPGGTILLRAHRAMESITLDRD
CHHHHHHHHHHHHHHCCCCEEEEECCCEECCCCCCCEECCCCEEEEEEHHHHHHHCCCCH
AAHLKDELMPRYAELVYNGYWFAPEREALQAMIDQTQQDVEGTVRLKLYKGNVIVVGRQS
HHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHCCCCEEEEEEEECCEEEEECCC
PKSLFDASVATFEDDAGAYDQKDAEGFIRLNALRLRLGAKRRG
CHHHHHHHHHEECCCCCCCCCCCCCCEEEEEEEEEEECCCCCC
>Mature Secondary Structure 
SDVKKVVLAYSGGLDTSVILKWLEETYGCEVVTFTADLGQGEELEPARRKAEAFGIREI
CCHHEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHCCHHHH
YVDDLREEFARDFVFPMFRANAVYEGEYLLGTSIARPLIAKRQVEIARETGADAVAHGAT
HHHHHHHHHHHHHHHHHHHCCEEECCCEEECHHHHHHHHHHHHHHHHHHCCCCHHCCCCC
GKGNDQVRFELGYYGLEPGIKVIAPWREWDLNSRERLLQYAERHGIDIEGKREEGGAPYS
CCCCCEEEEEEEEEECCCCCEEEECCHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEE
MDANLLHISYEGGVLEDTWTEAEEAMWLWTNAPEAAPDEPQYIDIAFAGGDPVAIDGERL
ECCCEEEEEECCCCCCCCCCCHHCEEEEEECCCCCCCCCCCEEEEEEECCCEEEECCCCC
SPAALLGRLNDLGARHGIGRIDIVENRYVGMKSRGCYETPGGTILLRAHRAMESITLDRD
CHHHHHHHHHHHHHHCCCCEEEEECCCEECCCCCCCEECCCCEEEEEEHHHHHHHCCCCH
AAHLKDELMPRYAELVYNGYWFAPEREALQAMIDQTQQDVEGTVRLKLYKGNVIVVGRQS
HHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHCCCCEEEEEEEECCEEEEECCC
PKSLFDASVATFEDDAGAYDQKDAEGFIRLNALRLRLGAKRRG
CHHHHHHHHHEECCCCCCCCCCCCCCEEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA